Threadfin bream (Nemipterus virgatus) is an important species in the trawl fishery of the South China Sea. In order to improve the size selectivity and exploitation pattern of single boat bottom otter trawls for N. virgatus, we tested four experimental codends with mesh sizes of 30, 35, 40 and 45 mm by applying the covered codend method in the South China Sea. We analyzed the catch data with double-bootstrapping technique to estimate the selectivity parameters, selectivity curves, delta selectivity curves and exploitation pattern indicators. The results demonstrate that with increasing mesh sizes in codends, 50% retention length (L50) increased, confidence intervals of the selectivity curves became sharper, and retention fraction of undersized individuals decreased. However, when considering the minimum conservation reference size of the target species (15.0 cm), the L50 values of codends tested were less than 15.0 cm, and their discarded percentages were all over 67%. Though the study shows that increasing mesh sizes will improve size selectivity, it should be further improved to achieve a sustainable trawl fishery for threadfin bream.

Being one of the primary fishing targets in Chinese pelagic fishing, Pacific saury (Cololabis saira) is distributed in the subtropical to temperate waters of the northwest Pacific Ocean. In order to explore its resource status, according to the catch at size and biological data of Northwest Pacific saury from 2014 to 2018, we conducted a performance test and a sensitivity analysis on length-based cohort analysis (LCA) model and biomass-based length-cohort analysis (B-LCA) model. Besides, we applied Monte Carlo method to estimate the model parameters, resource quantity, fishing mortality coefficients and maximum sustainable yield of Pacific saury. The results show that: 1) The LCA model and B-LCA model exhibited excellent fitting abilities at 5, 10 and 15 mm length intervals, with stronger fitting abilities at 5 mm interval. 2) LCA model performed better for fishery data in units of number, while B-LCA model performed better for fishery data in units of mass. 3) Both LCA and B-LCA models were sensitive to changes in growth factor ($b$) and asymptote length (${L}_{\infty }$), with higher sensitivity to b. 4) The average resource mass of Pacific saury from 2014 to 2018 estimated by LCA model was about 65.93×10⁴−171.51×10⁴ t; the fishing mortality coefficient was 0.529 2; the maximum sustainable yield was 37.73×10⁴ t. The average resource mass estimated by B-LCA model was about 47.88×10⁴−126.25×10⁴ t; the fishing mortality coefficient was 0.540 5; the maximum sustainable yield was $33.02\times {10}^{4}$ t. The maximum sustained production estimated by both models was lower than the average annual production of NPFC (North Pacific Ocean Commission) member countries ($40.98\times {10}^{4}$ t), indicating that the Pacific saury resources had been overfished from 2014 to 2018.

In order to understand the effect of fishing ban on Coilia nasus resources in the southern Duchang waters in the Poyang Lake, we conducted an electron probe microanalysis (EPMA) to study the migratory ecological characteristics of C. nasus in the waters before (2014) and after (2019) fishing ban. The results show that the otolith of all individuals had three significant fluctuation phases of Sr/Ca ratios of <3, 3–7 and <3 from the core to the edge, indicating a typical anadromous migration history. The number of samples (17 tails) after fishing ban was significantly higher than that before the ban (2 tails), and the body length of the former [(28.1±2.3) cm] was longer than that of the latter [(21.3±4.7) cm], which was also longer than that of the former reported before fishing ban. In addition, the length of low-value area on the otolith edge showed diverse characteristics [(125.0±63.6) μm, 80–170 μm in 2014, (173.5±73.6) μm, 20–290 μm in 2019], which suggests the phenomenon of mixed habitat of multiple upstream groups in the local waters. Considering that most of the individual gonads in the area were not mature, and that the southern Duchang waters (Dajishan to Hehe Township) are the only way for the population to enter and exit the central and southern waters of the Poyang Lake and the rivers connecting the lake (Such as Raohe River and Xinjiang River), it is strongly suggested that corresponding effective protection of this key habitat should be enhanced since the area mainly plays the ecological function of migration channel for C. nasus.

Johnius belangerii is an important fishery resource in the Zhanjiang Bay, and its population and distribution have a significant impact on fishery production and ecological balance. We investigated the life history traits and exploitation status of J. belangerii in the Zhanjiang Bay based on data from bottom trawl surveys conducted during 2016–2021. ELEFAN I, Pauly's empirical formula and length conversion capture curves were used to estimate growth parameters and mortality rates. Virtual population analysis (VPA) and Beverton-Holt model were used to assess the exploitation status. The results indicate that the body length of J. belangerii ranged from 27.03 mm to 182.00 mm, with mass ranging from 0.19 g to 137.34 g. The estimated asymptotic length (L_∞) was 185.85 mm, the growth coefficient (K) was 0.61, and the theoretical age at growth (t₀) was –0.27. The total mortality coefficient (Z), natural mortality coefficient (M) and fishing mortality coefficient (F) are 2.40, 0.75, and 1.65, respectively. The current catchable size (83.74 mm) was significantly smaller than the optimal size (131.95 mm), suggesting a large composition of juveniles and a trend towards population miniaturization. The exploitation rate (E) of 0.69 exceeded the maximum sustainable rate (E_max=0.63), indicating a state of overfishing and extensive exploitation. The study reveals the life history traits of J. belangerii and provides insights into its sustainable management and conservation.

Cetaceans, as apex predators in marine ecosystems, play a key role in maintaining ecological balance and stability. The Eden's whale (Balaenoptera edeni edeni) in Weizhou Island is the only baleen whales population that consistently appears in the waters near China. However, their specific distribution around Weizhou Island remains unclear. Due to their extensive range and active behavior, conducting stable tracking surveys via visual means is challenging. Thus, through environmental DNA (eDNA) technology, we assessed the distribution status of Eden's whales habitats around Weizhou Island during different periods (April 2022 and January 2023). The study reveals that in April, Eden's whales were visually observed and detected via eDNA in the hotspot distribution area (Between Weizhou Island and Xieyang Island) (n=3). They were also found in the southwest waters of Weizhou Island (n=2), with one site solely identified via eDNA. In January, Eden's whales were visually observed through eDNA in the hotspot distribution area (n=1) and detected through eDNA in the eastern waters off Weizhou Island (n=1). The findings indicate that compared with visual observation, eDNA technology exhibited higher sensitivity and could be utilized to verify the distribution of Eden's whales. Furthermore, potential hotspot distribution areas for Eden's whales were identified in the eastern and southwestern waters off Weizhou Island. In conclusion, this research validates the feasibility of utilizing eDNA technology for monitoring the distribution of Eden's whales around Weizhou Island. Besides, it provides further clarification regarding the distribution status of Eden's whales habitats around Weizhou Island, which supplies essential baseline information for the effective monitoring and scientific conservation of this population.

Multi-tissue fatty acid profile analysis reflects dietary information of organisms at different time scales, which plays an important indicative role in exploring trophic niche partitioning among large apex predators. In this study, we investigated the trophic niche partitioning of four shark species in the upper middle layer of tropical Atlantic Ocean (Prionace glauca, Isurus paucus, Pseudocarcharias kamoharai, I. oxyrinchus) by measuring the fatty acid profiles of their muscle and liver, to reveal the short-term and long-term feeding strategies and nutritional relationships. The results show that the four shark species had diverse degrees of dietary or habitat variation, and high trophic niche overlap was found between I. paucus and P. kamoharai, with intense dietary and habitat competition. However, there was trophic niche partitioning between I. paucus and I. oxyrinchus, indicating their dietary separation. P. glauca had relatively longer trophic niche width, indicating greater environmental plasticity, while the smaller one of P. kamoharai indicates dietary specialization. Comparison of liver and muscle fatty acid profiles reveals variable resource allocation patterns among sharks over time, with the highest overlap of trophic niche between the muscle of I. oxyrinchus and P. glauca, but separation of livers suggests intense competition for long-term feeding. The results help to understand the coexistence mechanisms of pelagic sharks.

Collichthys lucidus is an important small economic fish, and in order to understand its nutritional structure and dietary characteristics, we collected samples from the waters of the Pearl River Estuary in 2021 and 2022, and studied them from the perspectives of trophic niche, trophic structure index and feeding habit by on stable isotope technology. The results indicate that the δ¹³C and δ¹⁵N values of C. lucidus were (−18.74±1.41)‰ and (12.35±0.57)‰, respectively, with a trophic level of (3.25±0.17)‰. The δ¹³C value gradually increased when the body length was less than 100.00 mm, then decreased with the increase of body length. The δ¹⁵N value increased with the increase of body length when the body length was less than 110.00 mm, and the inflection point occurred at body length of 110.00–120.00 mm. The δ¹⁵N value was the lowest, and then increased with the increase of body length. When the body length was less than 100.00 mm and more than 120.00 mm, the trophic diversity was high, and the trophic niche width was large. When the body length was 100.00–120.00 mm, the redundancy and evenness were high, and the trophic niche distribution was uniform in the community, and the individuals with similar trophic characteristics accounted for the majority. Bayesian mixing model tracing reveals that the highest food contribution proportion for C. lucidus was from zooplankton. The research provides a theoretical basis for the resource conservation and management of C. lucidus.

Periodic high temperature (PHT) has a significant effect on biological function of shrimps in aquaculture. Gill, as the respiratory and metabolic organ of shrimp, plays a key role in maintaining the osmotic balance in fish body. However, its function is easily disturbed by environmental changes such as high temperature. In order to investigate effective strategies against PHT in shrimp, we investigated the effects of tributyrin (TB) on the morphology and physiological function of gill tissue of Litopenaeus vannamei under PHT [(34±1) ℃, 7 d] stress. The results show that PHT stress caused significant gill tissue damage and induced oxidative stress, which in turn caused endoplasmic reticulum stress, apoptosis and osmotic regulation dysfunction. With different feeding strategies (Feeding tributyrin for 7 d before stress, feeding tributyrin every day including 7 stressed days, and feeding tributyrin for 7 d after stress), the degree of gill tissue damage decreased significantly, so did the level of oxidative stress. In addition, tributyrin positively regulated the relative expression levels of oxidative stress, endoplasmic reticulum stress, apoptosis and osmoregulation genes which were induced by PHT. In particular, daily feeding tributyrin was effective in maintaining the morphological integrity of gill tissues and stable physiological functions.

Addition of oligosaccharide prebiotics into feeds can effectively enhance growth and immune-related performance of fish. Alginate oligosaccharide, an oligosaccharide prebiotic extracted from marine fucoidan, plays an important role in improving growth and immune performance of aquaculture animals. In order to investigate the effects of alginate oligosaccharide on the growth and immune performance of Lateolabrax maculatus juvenile, we conducted a 42-day experiment by adding alginate oligosaccharide to basic feed at doses of 0, 50, 100, and 200 mg·kg⁻¹ (alginate oligosaccharide: basic feed) to prepare experimental feed. The results show that the highest weight gain rate (WGR) [(96.50±7.95)%] and specific growth rate (SGR) [(1.73±0.09)%·d⁻¹] of juvenile were observed with alginate oligosaccharide addition of 100 mg·kg⁻¹. Expression of gh was significantly up-regulated in 100 mg·kg⁻¹ group (P<0.05). Meanwhile, the intestinal muscular thickness, villus height, villus width of juveniles, as well as the activities of trypsin (TRY), amylase (AMS) and lipase (LPS) in 100 mg·kg⁻¹ group were still higher than those of the control group (P<0.05). With increase of alginate oligosaccharide content, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and lysozyme (LZM) all increased significantly in liver of juvenile (P<0.05), while the malondialdehyde (MDA) content decreased significantly (P<0.05). Therefore, alginate oligosaccharide can improve the immune level of L. maculatus juvenile significantly. Besides, it has a certain promotion effect on its growth. The study provides theoretical references for the use of alginate oligosaccharide to promote the healthy growth of L. maculatus.

Excess carbon dioxide in atmosphere is absorbed by seawater and then form carbonic acid, which increases seawater acidity. To investigate the effects of seawater acidification on antioxidant and immunity in juvenile yellowfin tuna (Thunnus albacares), and to provide references for the protection of marine ecosystems and wild stocks of tuna, we took juvenile yellowfin tuna [Average body length of (18.21±1.09) cm and average body mass of (354.98±149.77) g] as research subjects, and set the pH gradients of 8.1, 7.6, 7.1 and 6.6 to determine the malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), peroxidase (POD), acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM) activities. The results show that after 48 h, the juveniles in pH 6.6 group showed mortality. Except for the control group, the activities of SOD, CAT, POD and LZM in gills were higher (P<0.05) than those of the other tissues, with the activities of SOD, CAT and POD peaking at pH 7.1, and the LZM activity peaking at pH 6.6; the GSH-Px activity in skin was higher than that in the other tissues (P<0.05), peaking at pH 7.1. In the liver, the MDA content was the most accumulated and the ACP activity was the highest (P<0.05), with the former peaking at pH 6.6 and the latter peaking at pH 8.1; the AKP activity in red muscle was significantly lower than that in the other tissues (P<0.05), reaching its lowest level at pH 7.1. The results show that there is some resistance of immune function and antioxidant defense system in juvenile yellowfin tuna when seawater pH is above 7.1, but has partial immune dysfunction when pH is lower than 7.1.

The extreme environmental features of low temperature and low oxygen on the Qinghai-Tibet Plateau provide a natural laboratory for the evolution of biological adaptability. The subfamily Schizothoracinae are widely distributed in the water bodies surrounding the Qinghai-Tibet Plateau, and has adapted to the unique highland environment through long-term evolutionary process. In this study, we focused on two high-altitude (>3 000 m) Schizothoracinae fish species (Schizothorax macropogon and S. waltoni) and one low-altitude (<1 000 m) ancestral Schizothoracinae fish species (S. prenanti) to conduct a transcriptome sequencing comparative analysis regarding the liver tissues, which is the main energy metabolism organ in fish species. This analysis reveals the key signaling pathways involved in the adaptation of highland Schizothoracinae to high-altitude environment. Comparing the liver transcriptomes of high-altitude with those of low-altitude Schizothoracinae, we identified 106 genes that shared differential changes in expression between the two high-altitude species. Among these genes, 66 were upregulated and 40 were downregulated. GO and KEGG enrichment analysis reveals that the most important pathways for high-altitude adaptation in Schizothoracinae include oxidation-reduction processes, pathways related to blood development, amino acid metabolism and steroid biosynthesis. Among them, genes related to oxidation-reduction and blood development (faxdc2, cpox, cyp51, and NADH-cytochrome b5 reductase 2) with high expression levels might play a crucial role in high-altitude adaptation. The study provides new insights into the molecular mechanisms underlying the adaptation of fish to high-altitude environment.

Feeding rhythms in fish show inter- and even intra-species variation. In order to detect the circadian feeding rhythm of Cyprinus carpio rubrofuscus and to provide a scientific basis for the establishment of standardized indoor aquaculture methods, we investigated the circadian feeding rhythm of C. carpio rubrofuscus and the relationship between the expression level of Agouti-related protein (AgRP) gene and circadian feeding rhythm under natural light. Besides, we measured the food intake of C. carpio rubrofuscus during the spring and autumn equinoxes in Guangzhou by segmented continuous feeding. We applied quantitative real-time PCR (qRT-PCR) to characterize the circadian expression profile of agrp genes in its brain, foregut, midgut and hindgut. The results show that the food intake of C. carpio rubrofuscus had a similar circadian rhythm, lowest at 8:00 in both spring and autumn equinoxes. Two feeding peaks appeared at 12:00 and 24:00 in spring equinox, and appeared at 12:00 and 20:00 in the autumn equinox. The qRT-PCR results show that the expressions of agrp1 and agrp2 genes had circadian rhythms, highest at 8:00 and 20:00 but lowest at 24:00 and 4:00 in brain. In foregut, midgut and hindgut, the highest agrp1 expression levels were observed at 16:00, 20:00 and 24:00, respectively, but the lowest level was observed at 8:00. The highest agrp2 expression levels were observed at 16:00, 24:00 and 24:00, but the lowest levels were observed at 12:00, 4:00 and 20:00. In conclusion, when the feeding intake level of C. carpio rubrofuscus was the lowest, the agrp1 gene had the highest expression level in brain to promote subsequent feeding behavior. Between the two feeding peaks (12:00−20:00), the expression levels of agrp1 reached the maximum in foregut, midgut and hindgut in turn. This trend was consistent with the movement of food in intestines. Therefore, the results suggest that agrp1 might be involved in the regulation of circadian feeding rhythm in C. carpio rubrofuscus. However, there was no correlation between expression level of agrp2 and feeding rhythm. It is indicated that agrp2 might have different functions in the evolutionary process.

Morphological traits reflect the diversity of different of large yellow croaker (Larimichthys crocea) of different geographic populations, so the morphological traits of L. crocea selected on the basis of different geographic populations are also slightly different. In order to provide references of phenotypic traits for the breeding of L. crocea and the selection of seedlings during artificial proliferation and release, we collected wild L. crocea from three locations (Zhanjiang, Guangdong Province; Xiamen, Fujian Province; Zhoushan, Zhejiang Province), measured their total length, body length, body height as well as caudal stalk height, as well as conducted correlation analysis, path analysis and multiple stepwise regression analysis. The coefficient of variation in body mass ranged from 17.44% to 19.79% among the three L. crocea populations. There was an extremely significant correlation among all the phenotypic traits (P<0.01). The numbers of phenotypic traits which played a major role in the populations of Zhanjiang, Xiamen and Zhoushan were 2 (Body length, body height), 3 (Total length, body length, body height), and 2 (Total length, body length), respectively. The multiple correlation coefficients (R) of phenotypic traits with significant effects on body mass for Zhanjiang, Xiamen and Zhoushan populations were 0.962, 0.915 and 0.964, respectively. After eliminating morphological traits with insignificant coefficients to body mass, we established the multiple regression equation by multiple regression analysis. The morphological traits retained by multiple regression analysis were consistent with those identified by path analysis.

Unmanned surface vessels (USVs) have autonomous navigation capacities under complex water surface environment, which is the basis for aquaculture operation, with great development prospects in intelligent aquaculture. Simultaneous localization and mapping (SLAM) technology provides real-time environmental information, playing a pivotal role in enabling autonomous navigation for USVs. However, water surfaces present low-texture scenes with insufficient distinct features, compounded by dynamic invalid features caused by surface ripples and reflections, which result in poor calculation accuracy and serious performance degradation of visual SLAM pose. To address this problem, the paper proposes a SLAM system oriented towards dynamic water surface environments based on visual semantics and point-line fusion. Firstly, the ORB-SLAM3 framework is enhanced by introducing a semantic segmentation thread to eliminate invalid water features using semantic masks, thereby mitigating dynamic water interference. Secondly, the system stability is enhanced by incorporating line features to propose a geometric-constraint-based line-matching method to improve the accuracy of waterline feature extraction and tracking. Moreover, the point-line feature fusion is utilized to enhance data association accuracy, resolving the deficiency of traditional SLAM algorithms in extracting features from low-texture water surfaces. The results of the USVInland dataset demonstrate that compared with ORB-SLAM3 and PL-SLAM algorithms, the positioning accuracy of the improved algorithm has averagely increased by average of 44.74% and 55.48% in straight-line navigation, and up to 76.60% and 70.15% in maneuvering navigation, which mitigates the impact of water surface disturbances on pose estimation effectively, and enhances the accuracy and robustness of visual SLAM systems under low-texture water environment.

Traditional freshness detection methods for aquatic products have problems such as great sample damage, trivial operation steps, low detection accuracy and efficiency. To solve these problems, in order to efficiently and accurately identify the freshness of small yellow croaker (Larimichthys polyactis), we proposed a freshness recognition model based on an improved DenseNet network. Firstly, we introduced the SENet attention mechanism module into each dense block module in the DenseNet network structure to achieve feature channel feature recalibration, enhance the network's extraction of current beneficial features, and eliminate irrelevant features. Secondly, we improved the first layer of the convolutional layer to enhance the network's non-linear ability and feature representation ability. To prevent the phenomenon of gradient vanishing during the training process, we used the PReLU activation function instead of the ReLU activation function of the original network. Finally, we conducted comparative experiments with the DenseNet network model and other classic neural network models. The experimental results show that the FishNet model based on transfer learning constructed in this paper has a recognition accuracy of 91.53% on the built L. polyactis freshness dataset. The model has high recognition accuracy and strong robustness, achieving efficient and accurate recognition of aquatic product freshness detection, and providing references for the development of intelligent freshness recognition systems.

Fenneropenaeus chinensis is nutritious with high economic value, but is easy to blacken during circulation and storage. Thus, in order to promote the standardized utilization of anti-blackening preservatives for shrimps and optimize their efficacy, we selected sodium metabisulfite and 4-hexylresorcinol (4-HR) as fundational components, both approved for use in the Standard for the Use of Food Additives (GB 2760−2014), and screened natural ingredients with anti-blackening effects, compounding them with sodium metabisulfite and 4-HR. Besides, by using phenoloxidase (PO) activity as a key indiex, we systematically determined the optimal ratio of this composite preservative through an orthogonal test. Then, we assessed its practical efficacy. The results show that the most effective formulation was: 3.0 g·L^–1 ascorbic acid, 5.0 g·L^–1 bamboo leaf antioxidant, 15.0 g·L^–1 sodium metabisulfite and 50.0 mg·L^–1 4-HR. The composite preservative demonstrated a remarkable capacity to mitigate blackening in F. chinensis compared with the control group. Moreover, application of the composite preservative resulted in significantly reduced levels of thiobarbituric acid reactants (TBARS), total volatile basic nitrogen (TVB-N) and total bacterial counts in F. chinensis during refrigeration, all significantly lower than those of the control group. Microbial diversity analysis reveales a pronounced inhibition of the dominant genus Photobacterium during the spoilage process of F. chinensis treated with the composite preservative. In conclusion, this composite preservative not only meets with the GB 2760 standards, but also exhibits exceptional anti-blackening and preservation effects, with good application prospects.

In order to explore the extended application of fish proteins and promote the high value utilization of brown-striped mackerel scad (Decapterus maruadsi), taking the brown-striped mackerel scad protein isolate limited enzymatic hydrolysate (BPILH) as raw material, we used glucose to conduct a glycation reaction to investigate the effects of different reaction time on the structural and functional properties of BPILH. The results show that glucose underwent glycation reaction with BPILH, and the degree of glycation reaction was positively correlated with reaction time and chromaticity. Structural properties results show that the glycation reaction reduced the α-helix and irregular coil content of BPILH and decreased its endogenous fluorescence intensity. In addition, high temperature treatment with long time during glycation reaction exposed the hydrophobic groups inside the protein molecules and increased their surface hydrophobicity. The solubility of glycation BPILH firstly increased and then decreased with the reaction time under acidic and alkaline conditions, in which the glycation solubility of product reached the highest value of (90.49±0.01)% at pH of 10 after 8-hour reaction. The emulsification of glycosylated BPILH increased with the reaction time. Comparied with the original mixture before reaction, the emulsification of the glycation product after 12-hour reaction increased by 24.28%, and both emulsification stability and oil-holding capacity showed an increase-decrease tendency with the reaction time, with the highest value of emulsification stability of (17.51±0.13) min at 1^st hour and oil-holding capacity of (2.19±0.21) g·g⁻¹ at 2^nd hour. The emulsification stability of glycation product increased by 24.28% with the reaction time. Therefore, long reaction time may have some negative effects on the functional properties, and this study provides theoretical references for the application of brown-striped mackerel scad protein isolate in food industry.

Development of efficient and safe extraction technology of astaxanthin from shrimp shells is crucial for transforming waste into valuable resources. In this study, astaxanthin in shrimp shells was extracted with deep eutectic solvent composed of lactic acid-choline chloride. A single-factor test was carried out to explore the influence of extraction conditions on the extraction rate, and a response surface test was adopted to optimize the extraction process. The mechanism of astaxanthin extraction with deep eutectic solvent was explored by physical and chemical analysis. The optimized process conditions were: molar ratio of 2.5∶1, water content of 9.5%, ultrasonic time of 30.5 min, and actual yield of 24.41 μg·g⁻¹. The Fourier transform infrared spectroscopy shows that there was no change in functional groups during deep eutectic solvent synthesis, but hydrogen bond might be the reason for effective extraction of astaxanthin, and viscosity would affect the extraction process of product.

The long-distance live fish transportation technology is the main method used in China's 'South-to-North Fish Transportation' program. During transportation process, fish are subjected to various environmental factors such as low temperature, salinity fluctuations, alkaline stress, etc. Using multi-omics integrated research methods, we conducted a multidimensional analysis of the stress response in fish transportation, including identification of differentially expressed genes, proteins and metabolites, as well as an analysis of regulatory networks and biomarkers associated with the stress response. These studies help to understand the health status of fish during transportation, optimization of temporary rearing and transportation conditions, as well as improvement of the quality and survival rate of live fish. The paper provides an overview of the recent advancements in omics research on various stress responses in fish during transportation, and provides a reference for the precise regulation of environmental indicators and in-depth investigation of the underlying mechanisms of stress response in live fish transportation.