Volume 20 Issue 3
Jun.  2024
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WU Yuhong, LIN Duanquan, LIU Kang, CHEN Yulei, ZHANG Lingjing, HE Wenxiong, SUN Lechang. Effects of glucose glycation on structural and functional properties of limited enzymatic hydrolysates from brown-striped mackerel scad (Decapterus maruadsi) protein isolate[J]. South China Fisheries Science, 2024, 20(3): 152-163. DOI: 10.12131/20240002
Citation: WU Yuhong, LIN Duanquan, LIU Kang, CHEN Yulei, ZHANG Lingjing, HE Wenxiong, SUN Lechang. Effects of glucose glycation on structural and functional properties of limited enzymatic hydrolysates from brown-striped mackerel scad (Decapterus maruadsi) protein isolate[J]. South China Fisheries Science, 2024, 20(3): 152-163. DOI: 10.12131/20240002
shu

Effects of glucose glycation on structural and functional properties of limited enzymatic hydrolysates from brown-striped mackerel scad (Decapterus maruadsi) protein isolate

  • 1.

    College of Marine Food and Bioengineering, Jimei University, Xiamen 361021, China

  • 2.

    National and Local Joint Engineering Research Center for Deep Processing Technology of Aquatic Products, Xiamen 361021, China

  • 3.

    Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China

  • 4.

    Xiamen a1 Snacks Lab Co., Ltd., Xiamen 361021, China

More Information
  • Received Date: January 02, 2024
  • Revised Date: February 11, 2024
  • Accepted Date: March 06, 2024
  • Available Online: March 12, 2024
    Graphical Abstract
    • Abstract

  • In order to explore the extended application of fish proteins and promote the high value utilization of brown-striped mackerel scad (Decapterus maruadsi), taking the brown-striped mackerel scad protein isolate limited enzymatic hydrolysate (BPILH) as raw material, we used glucose to conduct a glycation reaction to investigate the effects of different reaction time on the structural and functional properties of BPILH. The results show that glucose underwent glycation reaction with BPILH, and the degree of glycation reaction was positively correlated with reaction time and chromaticity. Structural properties results show that the glycation reaction reduced the α-helix and irregular coil content of BPILH and decreased its endogenous fluorescence intensity. In addition, high temperature treatment with long time during glycation reaction exposed the hydrophobic groups inside the protein molecules and increased their surface hydrophobicity. The solubility of glycation BPILH firstly increased and then decreased with the reaction time under acidic and alkaline conditions, in which the glycation solubility of product reached the highest value of (90.49±0.01)% at pH of 10 after 8-hour reaction. The emulsification of glycosylated BPILH increased with the reaction time. Comparied with the original mixture before reaction, the emulsification of the glycation product after 12-hour reaction increased by 24.28%, and both emulsification stability and oil-holding capacity showed an increase-decrease tendency with the reaction time, with the highest value of emulsification stability of (17.51±0.13) min at 1st hour and oil-holding capacity of (2.19±0.21) g·g−1 at 2nd hour. The emulsification stability of glycation product increased by 24.28% with the reaction time. Therefore, long reaction time may have some negative effects on the functional properties, and this study provides theoretical references for the application of brown-striped mackerel scad protein isolate in food industry.

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  • [1]
    农业农村部渔业渔政管理局, 全国水产技术推广总站, 中国水产学会. 2023中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2023: 39-41.
    [2]
    沈月新. 水产食品学[M]. 北京: 中国农业出版社, 2001: 21.
    [3]
    王伟, 刘俊荣, 马永生, 等. 等电点沉淀法鱼分离蛋白结构与功能变化研究进展[J]. 食品科学, 2015, 36(1): 250-255.
    [4]
    KLOST M, DRUSCH S. Functionalisation of pea protein by tryptic hydrolysis: characterisation of interfacial and functional properties[J]. Food Hydrocoll, 2019, 86(1): 134-140.
    [5]
    NISOV A, ERCILI-CURA D, NORDLUND E. Limited hydrolysis of rice endosperm protein for improved techno-functional properties[J]. Food Chem, 2020, 302: 125274. doi: 10.1016/j.foodchem.2019.125274
    [6]
    刘伟峰. 限制性酶解对蓝圆鲹分离蛋白功能特性的影响[D]. 厦门: 集美大学, 2020: 19.
    [7]
    YUAN F Z, AHMED I, LV L T, et al. Impacts of glycation and transglutaminase-catalyzed glycosylation with glucosamine on the conformational structure and allergenicity of bovine β-lactoglobulin[J]. Food Funct, 2018, 9(7): 3944-3955. doi: 10.1039/C8FO00909K
    [8]
    KATO A, SASAKI Y, FURUTA R, et al. Functional protein- polysaccharide conjugate prepared by controlled dry-heating of ovalbumin-dextran mixtures[J]. Agric Biol Chem, 1990, 54(1): 107-112.
    [9]
    LI Y L, HOU F, YANG Z T, et al. The growth of N-doped carbon nanotube arrays on sintered Al2O3 substrates[J]. Mater Sci Eng B, 2009, 158(1/2/3): 69-74.
    [10]
    ZHU D, DAMODARAN S, LUCEY J A. Physicochemical and emulsifying properties of whey protein isolate (WPI)-dextran conjugates produced in aqueous solution[J]. J Agric Food Chem, 2010, 58(5): 2988-2994. doi: 10.1021/jf903643p
    [11]
    MU L X, ZHAO M M, YANG B, et al. Effect of ultrasonic treatment on the graft reaction between soy protein isolate and gum acacia and on the physicochemical properties of conjugates[J]. J Agric Food Chem, 2010, 58(7): 4494-4499. doi: 10.1021/jf904109d
    [12]
    JIANG Z, BRODKORB A. Structure and antioxidant activity of Maillard reaction products from α-lactalbumin and β-lactoglobulin with ribose in an aqueous model system[J]. Food Chem, 2012, 133(3): 960-968. doi: 10.1016/j.foodchem.2012.02.016
    [13]
    YANG S, LEE S, PYO M C, et al. Improved physicochemical properties and hepatic protection of Maillard reaction products derived from fish protein hydrolysates and ribose[J]. Food Chem, 2017, 221: 1979-1988. doi: 10.1016/j.foodchem.2016.11.145
    [14]
    DECOURCELLE N, SABOURIN C, DAUER G, et al. Effect of the Maillard reaction with xylose on the emulsifying properties of a shrimp hydrolysate (Pandalus borealis)[J]. Food Res Int, 2010, 43(8): 2155-2160. doi: 10.1016/j.foodres.2010.07.026
    [15]
    DONG S Y, WEI B B, CHEN B C, et al. Chemical and antioxidant properties of casein peptide and its glucose Maillard reaction products in fish oil-in- water emulsions[J]. J Agric Food Chem, 2011, 59(24): 13311-13317. doi: 10.1021/jf203778z
    [16]
    董烨, 张益奇, 张晓頔, 等. 鳙鱼皮水解物美拉德反应产物抗氧化活性研究[J]. 核农学报, 2022, 36(11): 2199-2209.
    [17]
    马露燕. 低聚木糖糖基化豌豆蛋白构建微囊体系及其性能研究[D]. 郑州: 河南工业大学, 2023: 26.
    [18]
    WANG L H, SUN X, HUANG G Q, et al. Conjugation of soybean protein isolate with xylose/fructose through wet-heating Maillard reaction[J]. J Food Meas Charact, 2018, 12(4): 2718-2724. doi: 10.1007/s11694-018-9889-y
    [19]
    孙乐常, 刘伟峰, 林怡晨, 等. 碱性蛋白酶限制性酶解对蓝圆鲹分离蛋白功能特性的影响[J]. 食品科学, 2021, 42 (2): 23-29.
    [20]
    ZHANG Y Y, XU J M, ZHANG T, et al. Effect of carbon numbers and structures of monosaccharides on the glycosylation and emulsion stabilization ability of gelatin[J]. Food Chem, 2022, 389: 133128. doi: 10.1016/j.foodchem.2022.133128
    [21]
    WANG W L, ZHANG L, WANG Z Q, et al. Physicochemical and sensory variables of Maillard reaction products obtained from Takifugu obscurus muscle hydrolysates[J]. Food Chem, 2019, 290: 40-46. doi: 10.1016/j.foodchem.2019.03.065
    [22]
    詹梦涛, 娄水珠, 刘仙花, 等. 3, 5-二硝基水杨酸法测定液体糖中总糖含量[J]. 云南民族大学学报(自然科学版), 2020, 29(4): 317-321.
    [23]
    LIU C, WANG Z J, JIN H, et al. Effect of enzymolysis and glycosylation on the curcumin nanoemulsions stabilized by β-conglycinin: formation, stability and in vitro digestion[J]. Int J Biol Macromol, 2020, 142: 658-667.
    [24]
    LAEMMLI U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature, 1970, 227(5259): 680-685. doi: 10.1038/227680a0
    [25]
    WU Q, CAI Q F, TAO Z P, et al. Purification and characterization of a novel angiotensin I-converting enzyme inhibitory peptide derived from abalone (Haliotis discus hannai Ino) gonads[J]. Eur Food Res Technol, 2015, 240(1): 137-145. doi: 10.1007/s00217-014-2315-8
    [26]
    WANG Y, ZHOU Y, WANG X X, et al. Origin of high-pressure induced changes in the properties of reduced-sodium chicken myofibrillar protein gels containing CaCl2: physicochemical and molecular modification perspectives[J]. Food Chem, 2020, 319: 126535. doi: 10.1016/j.foodchem.2020.126535
    [27]
    CAO L, SU S, REGENSTEIN J M, et al. Ca2+-induced conformational changes of myosin from silver carp (Hypophthalmichthys molitrix) in Gelation[J]. Food Biophys, 2015, 10(4): 447-455. doi: 10.1007/s11483-015-9408-1
    [28]
    陈思如. 低聚木糖糖基化改性酪蛋白的功能特性及应用[D]. 长春: 吉林大学, 2022: 38-39.
    [29]
    GONG K J, SHI A M, LIU H Z, et al. Emulsifying properties and structure changes of spray and freeze-dried peanut protein isolate[J]. J Food Eng, 2016, 170: 33-40. doi: 10.1016/j.jfoodeng.2015.09.011
    [30]
    TAN E S, NGOH Y Y, GAN C Y. A comparative study of physicochemical characteristics and functionalities of pinto bean protein isolate (PBPI) against the soybean protein isolate (SPI) after the extraction optimisation[J]. Food Chem, 2014, 152: 447-455.
    [31]
    冷雪冬. 稻米米糠蛋白复合多糖脂肪替代物制备及应用研究[D]. 大庆: 黑龙江八一农垦大学, 2023: 5-6.
    [32]
    MARKOWICZ B D. Maillard reaction products in processed food: pros and cons[M]. London: IntechOpen, 2012: 281-299.
    [33]
    张杨翌. 糖基化改性对明胶结构和乳化性的影响研究[D]. 上海: 上海海洋大学, 2022: 16.
    [34]
    王可. 木质化鸡胸肉分级模型构建与过氧化氢体外氧化肌原纤维蛋白劣变机制及功能特性改善[D]. 泰安: 山东农业大学, 2023: 59-60.
    [35]
    XU Y J, ZHAO Y Q, WEI Z X, et al. Modification of myofibrillar protein via glycation: physicochemical characterization, rheological behavior and solubility property[J]. Food Hydrocoll, 2020, 105(7): 105852.
    [36]
    SHANMUGAM A, ASHOKKUMAR M. Ultrasonic preparation of stable flax seed oil emulsions in dairy systems: physicochemical characterization[J]. Food Hydrocoll, 2014, 39: 151-162. doi: 10.1016/j.foodhyd.2014.01.006
    [37]
    WONG B T, DAY L, AUGUSTIN M A. Deamidated wheat protein-dextran Maillard conjugates: effect of size and location of polysaccharide conjugated on steric stabilization of emulsions at acidic pH[J]. Food Hydrocoll, 2011, 25(6): 1424-1432. doi: 10.1016/j.foodhyd.2011.01.017
    [38]
    李琳. 限制性酶解结合糖基化处理对大豆分离蛋白结构与加工特性的影响[D]. 雅安: 四川农业大学, 2021: 36-37.
    [39]
    LIU G, LI W R, QIN X G, et al. Pickering emulsions stabilized by amphiphilic anisotropic nanofibrils of glycated whey proteins[J]. Food Hydrocoll, 2020, 101(4): 105503.
    [40]
    丁俭, 黄祯秀, 杨梦竹, 等. 食源蛋白水解物/多肽与糖类物质美拉德反应产物在食品应用中的研究进展[J]. 食品科学, 2023, 44(1): 305-318.
    [41]
    常雨晴. 带鱼多肽制备及美拉德反应对其性质影响研究[D]. 大连: 大连海洋大学, 2023: 25-26.
    [42]
    JACKSON M, MANTSCH H H. The use and misuse of FTIR spectroscopy in the determination of protein structure[J]. Crit Rev Biochem Mol Biol, 1995, 30(2): 95-120. doi: 10.3109/10409239509085140
    [43]
    LIU J H, FANG C H, XU X, et al. Structural changes of silver carp myosin glycated with konjac oligo- glucomannan: effects of deacetylation[J]. Food Hydrocoll, 2019, 91: 275-282. doi: 10.1016/j.foodhyd.2019.01.038
    [44]
    王秋野. 豌豆蛋白基植物肉的研制及特性分析[D]. 哈尔滨: 东北农业大学, 2021: 44-45.
    [45]
    STANIC-VUCINIC D, PRODIC I, APOSTOLOVIC D, et al. Structure and antioxidant activity of β-lactoglobulin-glycoconjugates obtained by high-intensity-ultrasound-induced Maillard reaction in aqueous model systems under neutral conditions[J]. Food Chem, 2013, 138(1): 590-599. doi: 10.1016/j.foodchem.2012.10.087
    [46]
    张燕鹏, 张曼君, 齐玉堂, 等. 微波-超声波协同影响菜籽蛋白糖基化改性[J]. 食品科学, 2017, 38(17): 114-119.
    [47]
    齐宝坤, 李杨, 王中江, 等. 不同品种大豆分离蛋白Zeta电位和粒径分布与表面疏水性的关系[J]. 食品科学, 2017, 38(3): 114-118.
    [48]
    LILIENTHAL S, DROTLEFF A M, TERNES W. Changes in the protein secondary structure of hen's egg yolk determined by Fourier transform infrared spectroscopy during the first eight days of incubation[J]. Poultry Sci, 2015, 94(1): 68-79. doi: 10.3382/ps/peu051
    [49]
    KUCHLYAN J, KUNDU N, BANIK D, et al. Spectroscopy and fluorescence lifetime imaging microscopy to probe the interaction of bovine serum albumin with graphene oxide[J]. Langmuir, 2015, 31(51): 13793-13801. doi: 10.1021/acs.langmuir.5b03648
    [50]
    KIM J, LEE Y. Effect of reaction pH on enolization and racemization reactions of glucose and fructose on heating with amino acid enantiomers and formation of melanoidins as result of the Maillard reaction[J]. Food Chem, 2008, 108(2): 582-592. doi: 10.1016/j.foodchem.2007.11.014
    [51]
    赵新淮等. 食品蛋白质[M]. 北京: 科学出版社, 2009: 138-139.
    [52]
    闫雨洁. 超声结合糖基化反应改性卵白蛋白及其理化性质与乳化性能研究[D]. 南宁: 广西大学, 2021: 19-20.
    [53]
    张玥, 李芳, 李菊梅, 等. 葡萄糖修饰苦杏仁粕谷蛋白糖基化反应条件的优化[J]. 食品研究与开发, 2022, 43(3): 94-101.
    [54]
    XUE F, LI C, ZHU X W, et al. Comparative studies on the physicochemical properties of soy protein isolate-maltodextrin and soy protein isolate-gum acacia conjugate prepared through Maillard reaction[J]. Food Res Int, 2013, 51(2): 490-495. doi: 10.1016/j.foodres.2013.01.012
    [55]
    刘杰. 糖基化明胶的制备及其在藻油微胶囊中的应用[D]. 重庆: 西南大学, 2023: 24-25.
    [56]
    WANG Y Y, ZHANG A Q, WANG Y X, et al. Effects of irradiation on the structure and properties of glycosylated soybean proteins[J]. Food Funct, 2020, 11(2): 1635-1646. doi: 10.1039/C9FO01879D
    [57]
    刘鑫慧. 蛋白酶对面筋蛋白糖基化产物特性及饼干品质的影响[D]. 郑州: 河南工业大学, 2023: 26-28.
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