Structure and evolution of mitochondrial control regions in Evynnis cardinalis

We determined the complete sequences of the mitochondrial DNA control regionsin Evynnis cardinalis using long polymerase chain reaction (PCR), and aligned them by Clustal X with 9 related Sparidae sequences in GenBank. Several conserved sequence blocks were identified in the mitochondrial DNA control regions, including extended terminal associated sequences (ETAS), blocks of central domain (CSB-F, CSB-E, CSB-D, CSB-C, CSB-B, CSB-A) and conserved sequence blocks (CSB1, CSB2, CSB3). Taking Paralabrax clathratus and Verasper moseri as outgroups, the molecular phylogenetic trees of Sparidae were constructed based on neighbour-joining and maximum-parsimony methods, which reveal that Sparidae is a monophyletic group, and E.cardinalis should be grouped with Pagrus. Therefore, it is necessary to reevaluate the taxonomic status of Evynnis and Pagrus.