Volume 7 Issue 5
Oct.  2011
Turn off MathJax
Article Contents
Abstract
References
Related Articles
RONG Chaozhen, ZU Guozhang, HU Jianhua, SUN Shouqi, SUN Tangli. Structure of mitochondrial DNA control region and genetic diversity of Misgurnus anguillicaudatus[J]. South China Fisheries Science, 2011, 7(5): 55-62. DOI: 10.3969/j.issn.2095-0780.2011.05.009
Citation: RONG Chaozhen, ZU Guozhang, HU Jianhua, SUN Shouqi, SUN Tangli. Structure of mitochondrial DNA control region and genetic diversity of Misgurnus anguillicaudatus[J]. South China Fisheries Science, 2011, 7(5): 55-62. DOI: 10.3969/j.issn.2095-0780.2011.05.009
shu

Structure of mitochondrial DNA control region and genetic diversity of Misgurnus anguillicaudatus

  • 1.

    College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

  • 2.

    Huaiyuan County Aquaculture Technology Extension Center of Anhui Province, Huaiyuan 233400, China

  • 3.

    Huaiyuan Fishery Science Technology Development Co., Ltd.of Anhui Province, Huaiyuan 233400, China

More Information
  • Received Date: March 24, 2011
  • Revised Date: May 02, 2011
    Graphical Abstract
    • Abstract

  • We compare and study the genetic diversity and structure of DNA control region of 4 Misgurnus anguillicaudatus populations by using PCR and DNA sequencing. The results show that the sequence of mitochondrial DNA control region is 918~920 bp.Among 32 sequences, a total of 56 polymorphic loci were detected, including 32 transitions, 19 transversions, 5 transitions-transversions and 36 haplotypes. The key sequences of extended terminal associated sequences (ETAS), central domains (CSB-A, B, C, D, E and F) and conserved sequence blocks (CSB-1, CSB-2 and CSB-3) were discovered. The haplotypic diversity (Hd), average genetic distance (Pi) and average number of pairwise differences (K) is 0.992, 0.012 and 10.698, respectively. The average Kimura 2-parameter distance, Fst value, Nm value and AMOVA reveal great genetic differentiation among the 4 M.anguillicaudatus populations. The NJ phylogenetic tree constructed on nucleotide sequence indicates that the 4 populations form into 2 monophyletic lineages, and cross and cluster among one another except Fanxian population.

    • FullText(HTML)
    • References (31)
  • [1]
    CANN R L, BROWN W M, WILSON A C. Polymorphic sites and the mechanism of evolution in human mitochondrial DNA[J]. Genetics, 1984, 106(2): 479-499. doi: 10.1093/genetics/106.3.479
    [2]
    ROSEL P E, DIZON A E, HAYGOOD M G. Variability of the mitochondrial control region in populations of the harbour porpoise, Phocoena phocoena, on interoceanic and regional scales[J]. Can J Fish Aquat Sci, 1995, 52(3): 1421-1429. doi: 10.1139/F95-118
    [3]
    ROSEL P E, HAYGOOD M G, PERRIN W F. Phylogenetic relationship among the true porpoise (Cetacean: Phocoenidae)[J]. Mol Phyl Evol, 1995, 4(4): 463-474. doi: 10.1006/mpev.1995.1043
    [4]
    孙玉华, 王伟, 刘思阳, 等. 中国胭脂鱼线粒体控制区遗传多样性分析[J]. 遗传学报, 2002, 29(9): 787-790. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg15Y3hiMjAwMjA5MDA3GghiazZiejkzZg%3D%3D

    SUN Yuhua, WANG Wei, LIU Siyang, et al. Genetic diversity research of mtDNA D-loop area from Chinese Myxocyprinus asiaticus[J]. Acta Genetica Sinica, 2002, 29(9): 787-790. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg15Y3hiMjAwMjA5MDA3GghiazZiejkzZg%3D%3D
    [5]
    杨金权, 胡雪莲, 唐文乔, 等. 长江口邻近水域刀鲚的线粒体控制区序列变异与遗传多样性[J]. 动物学杂志, 2008, 43(1): 8-15. doi: 10.3969/j.issn.0250-3263.2008.01.002

    YANG Jinquan, HU Xuelian, TANG Wenqiao, et al. mtDNA control region sequence variation and genetic diversity of Coilia nasus in Yangtze River estuary and its adjacent waters[J]. Chin J Zool, 2008, 43(1): 8-15. (in Chinese) doi: 10.3969/j.issn.0250-3263.2008.01.002
    [6]
    彭珊, 代应贵. 濒危鱼类稀有白甲鱼清水江种群mtDNA D-loop序列多态性[J]. 水产学报, 2009, 33(2): 196-200. doi: 10.3321/j.issn:1000-0615.2009.02.004

    PENG Shan, DAI Yinggui. Sequence polymorphism of mtDNA D-loop in the population of the endangered species Onychostom arara from the Qingshui River[J]. J Fish China, 2009, 33(2): 196-200. (in Chinese) doi: 10.3321/j.issn:1000-0615.2009.02.004
    [7]
    袁娟, 张其中, 李飞, 等. 铜鱼线粒体控制区的序列变异和遗传多样性[J]. 水生生物学报, 2010, 34(1): 9-19. doi: 10.3724/SP.J.1035.2010.00009

    YUAN Juan, ZHANG Qizhong, LI Fei, et al. mtDNA control rigion sequence variation and genetic diversity of Coreius heterodon (Bleeker) in the upper and middle sections of the Yangtz River[J]. Acta Hydaobiologica Sinica, 2010, 34(1): 9-19. (in Chinese) doi: 10.3724/SP.J.1035.2010.00009
    [8]
    刘海林, 章群, 唐优良, 等. 黄渤海松江鲈鱼线粒体控制区结构与序列多态性分析[J]. 海洋通报, 2010, 29(3): 283-288. doi: 10.3969/j.issn.1001-6392.2010.03.008

    LIU Hailin, ZHANG Qun, TANG Youliang, et al. Structure and genetic diversity of mtDNA D-Loop sequences among Trachidermus fasciatus stocks in Yellow Sea and Bohai Sea of China[J]. Mar Sci Bull, 2010, 29(3): 283-288. (in Chinese) doi: 10.3969/j.issn.1001-6392.2010.03.008
    [9]
    朱翠兰, 余为一, 陆依群, 等. 分布稻田的三种不同体色泥鳅组织LDH同工酶的研究[J]. 安徽农业大学报, 1996, 23(2): 155-159. doi: 10.1007/BF02009548

    ZHU Cuilan, YU Weiyi, LU Yiqun, et al. Study on characterization of the electrophoretic patterns of LDH isoenzymes of Migurnus anguillicaudatus distributed over bog[J]. J Anhui Agric Univ, 1996, 23(2): 155-159. (in Chinese) doi: 10.1007/BF02009548
    [10]
    常重杰, 周家荣, 余其兴. 两种泥鳅不同群体遗传变异的RAPD分析[J]. 动物学报, 2001, 47(1): 89-93. doi: 10.3969/j.issn.1674-5507.2001.01.015

    CHANG Chongjie, ZHOU Jiarong, YU Qixing. Genetic variation of two loach species revealed by RAPD analysis[J]. J Anim, 2001, 47(1): 89-93. (in Chinese) doi: 10.3969/j.issn.1674-5507.2001.01.015
    [11]
    曾柳根, 甘云飞, 王军花, 等. 鄱阳湖区泥鳅的微卫星DNA多态性分析[J]. 南昌大学学报: 理科版, 2008, 32(1): 84-88. doi: 10.3969/j.issn.1006-0464.2008.01.020

    ZENG Liugen, GAN Yunfei, WANG Junhua, et al. Genetic diversity of loaches (Misgurnus anguillicaudatus) from Poyang Lake determined by microsatellites DNA[J]. J Nanchang Univ: Science Edition, 2008, 32(1): 84-88. (in Chinese) doi: 10.3969/j.issn.1006-0464.2008.01.020
    [12]
    单磊. 长江流域二倍体泥鳅野生群体遗传多样性的微卫星分析[D]. 武汉: 华中农业大学, 2009. 10.7666/d.y1812498

    SHAN Lei. Population genetic diversity of natural diploid loach in the Yangtze River basin as revealed by microsatellite DNA analyses[D]. Wuhan: Huazhong Agricultural University, 2009. (in Chinese) 10.7666/d.y1812498
    [13]
    PEREZ-ENRIQUZE R, TANIGUCHI N. Genetic structure of red sea bream (Pagrus major) population of Japan and the southwest Pacific using microsatellite DNA marks[J]. Fish Sci, 1999, 65(1): 23-30. doi: 10.2331/fishsci.65.23
    [14]
    LIU Huanzhang, TZENG C S, TENG Huiyu. Sequence variations in the mitochondrial DNA control region and their implications for the phylogeny of the Cypriniformes[J]. Can J Zool, 2002, 80(6): 569-581 doi: 10.1139/z02-035
    [15]
    SACCONE C, PESOLE G, SBISA E. Structral elements highly preserved during the evolution of the D-loop containing region in vertebrate mitochodrial DNA[J]. Mol Evol, 1987, 26(4): 205-211.
    [16]
    郑冰蓉, 张亚平, 肖蘅. 鲤属鱼类mtDNA控制区(D-环区)序列的变异性分析[J]. 水产学报, 2002, 26(4): 289-294. doi: 10.1007/BF02099853

    ZHENG Bingrong, ZHANG Yaping, XIAO Heng. mtDNA control region of Cyprinus (D-loop region) sequence analysis of the variability[J]. J Fish China, 2002, 26(4): 289-294. (in Chinese) doi: 10.1007/BF02099853
    [17]
    刘焕章. 鱼类线粒体DNA控制区的结构与进化: 以鱼类为例[J]. 自然科学进展, 2002, 12(3): 266-270. doi: 10.3321/j.issn:1002-008X.2002.03.008

    LIU Huanzhang. The structure and evolution of mitochondrial DNA control region of fish: a case study to bitterlings[J]. Prog Natural Sci, 2002, 12(3): 266-270. (in Chinese) doi: 10.3321/j.issn:1002-008X.2002.03.008
    [18]
    BROWN G G. Structural conservation and variation in the D-loop-containing region of vertebrate mitochondrial DNA[J]. J Mol Biol, 1986, 192(2): 503-511. doi: 10.1016/0022-2836(86)90272-x
    [19]
    BROUGHTON R E, DOWLING T E. Length variation in mitochondrial DNA of the minnow Cyprinella spiloptera[J]. Genetics, 1994, 138(1): 179-190. doi: 10.1093/genetics/138.1.179
    [20]
    LEE W J, CONROY J, HOWELL W H, et al. Structure and evolution of teleost mitochondrial control regions[J]. J Mol Evol, 1995, 41(1): 54-66. doi: 10.1007/BF00174041
    [21]
    张燕, 张鹗, 何舜平. 中国鲿科鱼类线粒体DNA控制区结构及其系统发育分析[J]. 水生生物学报, 2003, 27 (5): 463-467. doi: 10.3321/j.issn:1000-3207.2003.05.004

    ZHANG Yan, ZHANG E, HE Shunping. Studies on the Structure of the control region of the Bagridae in China and its phylogentic significance[J]. Acta Hydaobiologica Sinica, 2003, 27(5): 463-467. (in Chinese) doi: 10.3321/j.issn:1000-3207.2003.05.004
    [22]
    唐琼英, 刘焕章, 杨秀平, 等. 沙鳅亚科鱼类线粒体DNA控制区结构分析及系统发育关系的研究[J]. 水生生物学报, 2009, 26(6): 645-653. http://ssswxb.ihb.ac.cn/article/id/6ab96820-95a7-44db-920c-235de6ab9a17

    TANG Qiongying, LIU Huanzhang, YANG Xiuping, et al. Studies on the struction of the mitochondrial DNA control region and phylogenetic relationship of the subfamily Botiinae[J]. Acta Hydaobiologica Sinica, 2009, 26(6): 645-653. (in Chinese) http://ssswxb.ihb.ac.cn/article/id/6ab96820-95a7-44db-920c-235de6ab9a17
    [23]
    SOUTHERN S O, SOUTHERN P J, DIZON A E. Molecular characterization of a cloned dolphin mitochondrial genome[J]. J Mol Evol, 1988/1989, 28(1/2): 32-42. doi: 10.1007/BF02143495
    [24]
    SBISA E, TANZARIELLO F, REYES A, et al. Mammalian mitochondrial D-loop region structural analysis: identification of new conserved sequences and their functional and evolutionary implications[J]. Gene, 1997, 205(1/2): 125-140. doi: 10.1016/s0378-1119(97)00404-6
    [25]
    SACCONE C, PESOLE G, SBISA E. The main regulatory region of mammalian mitochondrial DNA: structure-function model and evolutionary pattern[J]. J Mol Evol, 1991, 33(1): 83-91. doi: 10.1007/BF02100199
    [26]
    曾青兰, 刘焕章. 大口胭脂鱼线粒体DNA控制区序列的研究[J]. 湖北大学学报: 自然科学版, 2001, 23(3): 261-264. doi: 10.3969/j.issn.1000-2375.2001.03.021

    ZENG Qinglan, LIU Huanzhang. Study on mitochondrial DNA control region of the Ictiobus cyprinellus[J]. J Hubei Univ: Natural Science, 2001, 23(3): 261-264. (in Chinese) doi: 10.3969/j.issn.1000-2375.2001.03.021
    [27]
    赵凯, 李军祥, 张亚平, 等. 青海湖裸鲤mtDNA遗传多样性的初步研究[J]. 遗传, 2001, 23(5): 445-448. https://xueshu.baidu.com/usercenter/paper/show?paperid=00d28978b80365611304219818059c47&site=xueshu_se

    ZHAO Kai, LI Junxiang, ZHANG Yaping, et al. Mitochondrial DNA diversity in naked carps in Qinghai-lake (Gymnocypris prezwalskii prezwalskii)[J]. Hereditas, 2001, 23(5): 445-448. (in Chinese) https://xueshu.baidu.com/usercenter/paper/show?paperid=00d28978b80365611304219818059c47&site=xueshu_se
    [28]
    赵刚, 周剑, 杜军, 等. 长薄鳅(Leptobotia elongata)线粒体DNA控制区遗传多样性研究[J]. 西南农业学报, 2010, 23(3): 930-937. doi: 10.3969/j.issn.1001-4829.2010.03.068

    ZHAO Gang, ZHOU Jian, DU Jun, et al. Genetic diversity of elongate loach (Leptobotia elongata) inferred from mitochondrial DNA control region[J]. Southwest China J Agric Sci, 2010, 23(3): 930-937. (in Chinese) doi: 10.3969/j.issn.1001-4829.2010.03.068
    [29]
    杨博, 陈小勇, 杨君兴. 浪白鱼线粒体DNA控制区结构和种群遗传多样性分析[J]. 动物学研究, 2008, 29(4): 379-385. doi: 10.3321/j.issn:0254-5853.2008.04.005

    YANG Bo, CHEN Xiaoyong, YANG Junxing. Structure of the mitochondrial DNA control region and population genetic diversity analysis of Anabarilius grahami (Regan)[J]. Zool Res, 2008, 29(4): 379-385. (in Chinese) doi: 10.3321/j.issn:0254-5853.2008.04.005
    [30]
    代应贵, 韩雪, 张晓杰. 小口白甲鱼都柳江种群mtDNA D环的序列变异及遗传多样性[J]. 动物学杂志, 2010, 45(2): 115-120. https://xueshu.baidu.com/usercenter/paper/show?paperid=a528705686b212f0d25207fde0a875cd&site=xueshu_se&hitarticle=1

    DAI Yinggui, HAN Xue, ZHANG Xiaojie. Genetic diversity and sequence variation of mtDNA D-loop in the population of vulnerable species Onychostoma lini from the Duliu River[J]. China J Zool, 2010, 45(2): 115-120. (in Chinese) https://xueshu.baidu.com/usercenter/paper/show?paperid=a528705686b212f0d25207fde0a875cd&site=xueshu_se&hitarticle=1
    [31]
    SONG Zhaobin, SONG Jun, YUE Bisong. Population genetic diversity of Prenant's schizothoracin, Schizothorax prenanti, inferred from the mitochondrial DNA control region[J]. Environ Biol Fish, 2008, 81(3): 247-252. doi: 10.1007/s10641-007-9197-6
    • Related Articles

  • Related Articles

    [1]ZHONG Zhanyou, DENG Hong, KOU Chunni, CHEN Weitao, WU Zhi, LI Yuefei, XIA Yuguo, LI Huifeng, LI Jie, ZHU Shuli. Research on fish diversity in Xijiang Rare Fish Provincial Nature Reserve based on environmental DNA technology[J]. South China Fisheries Science. DOI: 10.12131/20240173
    [2]ZHENG Deyu, GUO Yijia, YANG Tianyan, GAO Tianxiang, ZHENG Yao, YUAN Donghao, SI Shujin. Genetic diversity analysis of Sillago japonica based on mitochondrial DNA ND2 gene[J]. South China Fisheries Science, 2019, 15(5): 84-91. DOI: 10.12131/20190042
    [3]KUANG Tianxu, SHUAI Fangmin, CHEN Weitao, LI Xinhui. Genetic diversity and population structure of Carassius auratus in Xijiang River[J]. South China Fisheries Science, 2018, 14(5): 29-35. DOI: 10.3969/j.issn.2095-0780.2018.05.004
    [4]LI Min, LI Yufang, ZHANG Peng, CHEN Zuozhi. Analysis of population genetic structure of bullet tuna(Auxis rochei) in the South China Sea based on mitochondrial control region sequences[J]. South China Fisheries Science, 2016, 12(4): 88-95. DOI: 10.3969/j.issn.2095-0780.2016.04.011
    [5]MO Yanxiu, WANG Xiaoqing, MO Yongliang. RAPD analysis of genetic diversity of Leiocassis longirostris[J]. South China Fisheries Science, 2010, 6(6): 77-80. DOI: 10.3969/j.issn.1673-2227.2010.06.014
    [6]ZHU Caiyan, JIANG Shigui, ZHANG Dianchang, XIA Junhong, SU Tianfeng. Polymorphism study of the mitochondrial DNA D-loop sequences of original population of mud carp[J]. South China Fisheries Science, 2008, 4(1): 32-35.
    [7]LI Lihao, YU Dahui, HUANG Guiju, DU Bo, FU Yun, TONG Xin, GUO Yihui, YE Wei. Genetic diversity of selected GIFT strains of Oreochromis niloticus[J]. South China Fisheries Science, 2007, 3(5): 40-48.
    [8]ZHOU Fa-lin, JIANG Shi-gui, JIANG Yong-jie, HUANG Jian-hua, MA Zhi-ming. Polymorphism of mtDNA 16S rRNA gene and control region sequence in Penaeus monodon of Sanya, Hainan[J]. South China Fisheries Science, 2006, 2(6): 13-18.
    [9]GONG Jinbo, SU Tianfeng, XIA Junhong, GONG Shiyuan, JIANG Shigui. Polymorphism study of the mitochondrial DNA D-loop control region sequences from black porgy Acanthopagrus schlegeli, in the costal waters of China[J]. South China Fisheries Science, 2006, 2(4): 24-30.
    [10]JIANG Yongjie, ZHOU Falin, HUANG Jianhua, MA Zhiming, JIANG Shigui. Analysis on genetic diversity of mtDNA control region sequences of the wild population of Peneanus monodon from Shenzhen′s sea area[J]. South China Fisheries Science, 2006, 2(1): 54-57.

  • Cited by

    Periodical cited type(9)

    1. 蒲艳，田辉伍，陈大庆，段辛斌，刘绍平，汪登强. 长江中上游圆筒吻鮈群体线粒体Cyt b遗传多样性分析. 淡水渔业. 2019(01): 14-19 .
    2. 白晓慧，杨华，孟一耕，王娜，姜巨峰，吴会民，冯守明. 大鳞副泥鳅群体线粒体DNA D-loop序列遗传变异分析. 水产科学. 2018(05): 658-664 .
    3. 邱楚雯，王韩信. 泥鳅属鱼类遗传多样性的研究进展. 水产科技情报. 2017(02): 78-82+86 .
    4. 刘伟，代应贵，袁振兴，孙际佳，刘丽. 都柳江粗唇种群线粒体DNA Cyt b基因和D-loop序列组成及遗传多样性. 淡水渔业. 2016(03): 10-15+51 .
    5. 董坡，代应贵，尹邦一. 长脂拟鲿种群Cyt b基因序列的变异及遗传多样性. 贵州农业科学. 2015(06): 138-142 .
    6. 沈静轶，薛良义，沈伟良，何萌萌. 3个养殖大黄鱼群体线粒体DNA控制区遗传多样性分析. 生物学杂志. 2015(06): 30-33+59 .
    7. 董新培，穆淑梅，周楠，康现江，罗青，白俊杰. 不同地理群体乌鳢线粒体DNA控制区结构分析及遗传多样性. 水产学报. 2014(09): 1277-1285 .
    8. 李鹏飞，贺舟挺，徐开达，朱文斌，薛利建，张洪亮. 基于COⅠ基因和D-loop序列的东海鳓鱼种质资源遗传变异研究. 浙江海洋学院学报(自然科学版). 2013(02): 93-98 .
    9. 苏天凤，江世贵，马之明，李夏. 二长棘犁齿鲷线粒体DNA控制区结构和进化. 南方水产科学. 2012(06): 1-8 . 本站查看

    Other cited types(11)

Catalog

    Get Citation
    PDF
    XML
    Article views (4695) PDF downloads (2727) Cited by(20)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return