Volume 7 Issue 5
Oct.  2011
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RONG Chaozhen, ZU Guozhang, HU Jianhua, SUN Shouqi, SUN Tangli. Structure of mitochondrial DNA control region and genetic diversity of Misgurnus anguillicaudatus[J]. South China Fisheries Science, 2011, 7(5): 55-62. DOI: 10.3969/j.issn.2095-0780.2011.05.009
Citation: RONG Chaozhen, ZU Guozhang, HU Jianhua, SUN Shouqi, SUN Tangli. Structure of mitochondrial DNA control region and genetic diversity of Misgurnus anguillicaudatus[J]. South China Fisheries Science, 2011, 7(5): 55-62. DOI: 10.3969/j.issn.2095-0780.2011.05.009
shu

Structure of mitochondrial DNA control region and genetic diversity of Misgurnus anguillicaudatus

  • 1.

    College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

  • 2.

    Huaiyuan County Aquaculture Technology Extension Center of Anhui Province, Huaiyuan 233400, China

  • 3.

    Huaiyuan Fishery Science Technology Development Co., Ltd.of Anhui Province, Huaiyuan 233400, China

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  • Received Date: March 24, 2011
  • Revised Date: May 02, 2011
    Graphical Abstract
    • Abstract

  • We compare and study the genetic diversity and structure of DNA control region of 4 Misgurnus anguillicaudatus populations by using PCR and DNA sequencing. The results show that the sequence of mitochondrial DNA control region is 918~920 bp.Among 32 sequences, a total of 56 polymorphic loci were detected, including 32 transitions, 19 transversions, 5 transitions-transversions and 36 haplotypes. The key sequences of extended terminal associated sequences (ETAS), central domains (CSB-A, B, C, D, E and F) and conserved sequence blocks (CSB-1, CSB-2 and CSB-3) were discovered. The haplotypic diversity (Hd), average genetic distance (Pi) and average number of pairwise differences (K) is 0.992, 0.012 and 10.698, respectively. The average Kimura 2-parameter distance, Fst value, Nm value and AMOVA reveal great genetic differentiation among the 4 M.anguillicaudatus populations. The NJ phylogenetic tree constructed on nucleotide sequence indicates that the 4 populations form into 2 monophyletic lineages, and cross and cluster among one another except Fanxian population.

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