Volume 11 Issue 2
Apr.  2015
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HU Yuting, HU Wang, LING Jun, JIANG He, DUAN Guoqing, PAN Tingshuang. Comparative and phylogenetic analysis of mitochondrial Cytochrome b gene and control region from natural triploid (Carassius auratus gibelio) in Chuzhou[J]. South China Fisheries Science, 2015, 11(2): 101-108. DOI: 10.3969/j.issn.2095-0780.2015.02.015
Citation: HU Yuting, HU Wang, LING Jun, JIANG He, DUAN Guoqing, PAN Tingshuang. Comparative and phylogenetic analysis of mitochondrial Cytochrome b gene and control region from natural triploid (Carassius auratus gibelio) in Chuzhou[J]. South China Fisheries Science, 2015, 11(2): 101-108. DOI: 10.3969/j.issn.2095-0780.2015.02.015
shu

Comparative and phylogenetic analysis of mitochondrial Cytochrome b gene and control region from natural triploid (Carassius auratus gibelio) in Chuzhou

  • Fishery Research Institute of Anhui Academy of Agricultural Sciences, Hefei 230031, China

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  • Received Date: June 04, 2014
  • Revised Date: June 26, 2014
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    • Abstract

  • The complete sequences of mitochondrial cytochrome b (Cyt b) gene and control region (D-loop) were amplified and sequenced in Carassius auratus gibelio, a natural triploid crucian carp in Chuzhou City of Anhui Province. No nucleotide variation sites were found in all sequences among 14 samples. The 1 141 bp Cyt b gene contained 29.2%, 27.8%, 28.5% and 14.5% of T, C, A and G, respectively; while 924 bp D-loop sequence contained 33.2%, 20.1%, 32.6% and 14.1% of T, C, A and G, respectively. The contents of A+T (57.5%, 65.8%) were significantly greater than those of G+C (42.3%, 34.2%) and G was the lowest among four nucleotides, the similar case as other aquatic animals. The key sequences of extended terminal associated sequences (ETAS), central domains (CSB-D, E and F) and conserved sequence blocks (CSB-1, CSB-2 and CSB-3) were identified. There was high sequence similarity between C.auratus gibelio in Chuzhou and other Carassius species recorded in GenBank based on Cyt b (93%~100%) and D-loop (94%~99%) sequences, respectively. The phylogenetic trees constructed by NJ and MP methods based on the two sequences indicated that the closest phylogenetic relationship was between C.auratus gibelio in Chuzhou and that in Fangzheng, while the farthest one was between C.auratus gibelio in Chuzhou and crucian carps in Japan.

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  • [1]
    张克俭, 万全, 李公行, 等. 滁州鲫的形态学和血清蛋白电泳谱型[J]. 水产学报, 1996, 20(4): 352-356. https://xueshu.baidu.com/usercenter/paper/show?paperid=be53c9802dddecec1d656391de70e631&site=xueshu_se
    [2]
    张克俭, 万全, 李公行, 等. 滁州鲫染色体组型的研究[J]. 中国水产科学, 1995, 2(4): 8-15. doi: 10.3321/j.issn:1005-8737.1995.04.006
    [3]
    徐广友, 万全, 袁兆祥, 等. 滁州鲫雌核发育的细胞学研究[J]. 安徽农业大学学报, 2009, 36(4): 533-537.
    [4]
    凌武海, 任信林. 天然滁州鲫氨基酸成分分析与营养价值评价[J]. 水产养殖, 2011, 32(1): 50-52. doi: 10.3969/j.issn.1004-2091.2011.01.013
    [5]
    姚继花, 楼允东, 江涌. 我国六个地区银鲫种群线粒体DNA多态性研究[J]. 水产学报, 1998, 22(4): 289-295. doi: 10.1017/S0266078400010713
    [6]
    凌武海, 马明发, 袁兆祥, 等. 滁州鲫、方正银鲫和彭泽鲫分子遗传差异的分析[J]. 安徽农业科学, 2009, 37(28): 13482-13483, 13485. doi: 10.3969/j.issn.0517-6611.2009.28.023
    [7]
    肖武汉, 张亚平. 鱼类线粒体DNA的遗传与进化[J]. 水生生物学报, 2000, 24(4): 384-391. doi: 10.3321/j.issn:1000-3207.2000.04.014
    [8]
    郭新红, 刘少军, 刘巧, 等. 鱼类线粒体DNA研究新进展[J]. 遗传学报, 2004, 31(9): 983-1000. https://xueshu.baidu.com/usercenter/paper/show?paperid=25713e1ff4e884760e4e4364b31966b8&site=xueshu_se&hitarticle=1
    [9]
    XIAO Wuhan, ZHANG Yaping, LIU Huanzhang. Molecular systematics of Xenocyprinae (Teleostei: Cyprinidae): taxonomy, biogeography, and coevolution of a special group restricted in East Asia[J]. Mol Phylogenet Evol, 2001, 18(2): 163-173. doi: 10.1006/mpev.2000.0879
    [10]
    PERDICES A, DOADRIO I. The molecular systematics and biogeography of the European cobitids based on mitochondrial DNA sequences[J]. Mol Phylogenet Evol, 2001, 19(3): 468-478. doi: 10.1006/mpev.2000.0900
    [11]
    TANG Qiongying, LIU Huanzhang, MAYDEN R, et al. Comparison of evolutionary rates in the mitochondrial DNA cytochrome b gene and control region and their implications for phylogeny of the Cobitoidea (Teleostei: Cypriniformes)[J]. Mol Phylogenet Evol, 2006, 39(2): 347-357. doi: 10.1016/j.ympev.2005.08.007
    [12]
    YU Meiling, HE Shunping. Phylogenetic relationships and estimation of divergence times among Sisoridae catfishes[J]. Sci China Life Sci, 2012, 55(4): 312-320. doi: 10.1007/s11427-012-4305-z
    [13]
    ANG Jinqun, TANG Wenqiao, LIAO Teyu, et al. Phylogeographical analysis on Squalidus argentatus recapitulates historical landscapes and drainage evolution on the island of Taiwan and mainland China[J]. Int J Mol Sci, 2012, 13(12): 1405-1425. doi: 10.3390/ijms13021405
    [14]
    ZHAO Kai, DUAN Ziyuan, PENG Zuogang, et al. Phylogeography of the endemic Gymnocypris chilianensis (Cyprinidae): sequential westward colonization followed by allopatric evolution in response to cyclical Pleistocene glaciations on the Tibetan Plateau[J]. Mol Phylogenet Evol, 2011, 59(2): 303-310. doi: 10.1016/j.ympev.2011.02.001
    [15]
    AVISE J C. Gene trees and organismal histories: a phylogenetic approach to population biology[J]. Evolution, 1989, 43(6): 1192-1208. doi: 10.2307/2409356
    [16]
    YANG Guang, XIAO Mingsong, YU Yanyan, et al. Genetic variation at mtDNA and microsatellite loci in Chinese longsnout catfish (Leiocassis longirostris)[J]. Mol Biol Rep, 2012, 39(5): 4605-4617. doi: 10.1007/s11033-011-1252-x
    [17]
    ALTER S E, PALUMBI S R. Comparing evolutionary patterns and variability in the mitochondrial control region and Cytochrome b in three species of baleen whales[J]. J Mol Evol, 2009, 68(1): 97-111. doi: 10.1007/s00239-008-9193-2
    [18]
    LUNT D H, WHIPPLE L E, HYMAN B C. Mitochondrial DNA variable number tandem repeats (VNTRs): utility and problems in molecular ecology[J]. Mol Ecol, 1998, 7(11): 1441-1455. doi: 10.1046/j.1365-294x.1998.00495.x
    [19]
    PEREIRA S L, GRAU E T, WAJNTAL A. Molecular architecture and rates of DNA substitutions of the mitochondrial control region of cracid birds[J]. Genome, 2004, 47(3): 535-545. doi: 10.1139/g04-009
    [20]
    LIU Guangxun, ZHOU Jian, ZHOU Dinggang. Mitochondrial DNA reveals low population differentiation in elongate loach, Leptobotia elongata (Bleeker): implications for conservation[J]. Environ Biol Fish, 2012, 93(1): 393-402. doi: 10.1007/s10641-011-9927-7
    [21]
    李娜, 陈少波, 谢起浪, 等. 闽浙地区香鱼线粒体Cyt b基因和D-loop区序列多态性分析[J]. 遗传, 2008, 30(7): 919-925. doi: 10.3321/j.issn:0253-9772.2008.07.018
    [22]
    赵亮, 谢本贵, 刘志瑾, 等. 太湖新银鱼线粒体D-loop和Cyt b片段序列结构与进化速率比较[J]. 动物学杂志, 2010, 45(2): 27-38. https://www.doc88.com/p-9971835339647.html
    [23]
    GALTIER N, GOUY M, GAUTIER C. Seaview and Phylo_ win: two graphic tools for sequence alignment and molecular phylogeny[J]. Comput Appl Biosci, 1996, 12(6): 543-548. doi: 10.1093/bioinformatics/12.6.543
    [24]
    THOMPSON J D, GIBSON T J, PLEWNIAK F. The Clustal X windows interface: flexible strategies for multiple sequences alignment aided by quality analysis tools[J]. Nucleic Acids Res, 1997, 25(4): 4876-4882. doi: 10.1093/nar/25.24.4876
    [25]
    KUMAR S, DUDLEY J, NEI M, et al. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences[J]. Brief Bioinform, 2008, 9(4): 299-306. doi: 10.1093/bib/bbn017
    [26]
    HOCHACHKA P, MOMMSEN T P. Biochemistry and molecular biology of fish[M]. London: Elsevier Science Publishers, 1993: 1-38. https://www.sciencedirect.com/bookseries/biochemistry-and-molecular-biology-of-fishes
    [27]
    CANTATORE P, ROBERTI M, PESOLE G, et al. Evolutionary analysis of cytochrome b sequences in some perciformes: evidence for a slower rate of evolution than in mammals[J]. J Mol Evol, 1994, 39(6): 589-597. doi: 10.1007/BF00160404
    [28]
    刘焕章. 鱼类DNA控制区的结构和进化: 以鳑鲏为例[J]. 自然科学进展, 2002, 12(3): 266-270. doi: 10.3321/j.issn:1002-008X.2002.03.008
    [29]
    唐琼英, 刘焕章, 杨秀平. 沙鳅亚科鱼类线粒体DNA控制区结构分析及系统发育关系的研究[J]. 水生生物学报, 2005, 29(6): 645-653. doi: 10.3321/j.issn:1000-3207.2005.06.008
    [30]
    刘良国, 杨品红, 王文彬, 等. 洞庭青鲫与其他六个鲫鱼品系线粒体DNA控制区的比较分析[J]. 水生生物学报, 2010, 34(2): 378-387. doi: 10.3724/SP.J.1035.2010.00378
    [31]
    苏天凤, 江世贵, 马之明, 等. 二长棘犁齿鲷线粒体DNA控制区结构和进化[J]. 南方水产科学, 2012, 8(6): 1-8. doi: 10.3969/j.issn.2095-0780.2012.06.001
    [32]
    马明发, 张克俭, 凌武海, 等. 三种群银鲫形态特征的比较研究[J]. 河北渔业, 2009(4): 17-19. doi: 10.3969/j.issn.1004-6755.2009.04.005
    [33]
    程磊, 常玉梅, 鲁翠云, 等. 鲫属鱼类DNA条码及种与亚种划分[J]. 动物学研究, 2012, 33(5): 463-472. doi: 10.3724/SP.J.1141.2012.05463
    [34]
    王姝妍. 古环境的变化和人工选择对鲫属鱼类遗传结构的影响[D]. 合肥: 中国科学技术大学, 2013. http://www.51papers.com/lw/70/8/wz1915427.htm
    [35]
    乐佩琦. 硬骨鱼纲· 鲤形目· 下卷[M]. 北京: 科学技术出版社, 2000: 427-434. https://www.las.ac.cn/front/book/detail?id=91264d2d652fd346c5ed4c5ceb712232
    [36]
    HOSOYA K. Cyprinidae[M]//NAKABO T. Fishes of Japan with pictorial keys to the species. Tokyo: Tokai University Press, 2002: 253-254.
    [37]
    MURAKAMI M, MATSUBA C, FUJITANI H. The maternal origins of the triploid ginbuna (Carassius auratus langsdorfi): phylogenetic relationships within the C. auratus taxa by partial mitochondrial D-loop sequencing[J]. Genes Genet Syst, 2001, 76(1): 25-32. doi: 10.1266/ggs.76.25
    [38]
    付小全, 曾柳根, 洪一江, 等. 天然三倍体鲫鱼突变体萍乡肉红鲫线粒体细胞色素b基因序列分析[J]. 动物学杂志, 2009, 44(2): 97-101. http://dwxzz.ioz.ac.cn/dwxb/article/abstract/20090217?st=article_issue
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