WANG Wenhao, DONG Hongbiao, SUN Yongxu, CAO Ming, DUAN Yafei, LI Hua, LIU Qingsong, ZHANG Jiasong. Anesthetic effects of MS-222 and eugenol in simulated transportation of juvenile largemouth bass (Micropterus salmoides)[J]. South China Fisheries Science, 2018, 14(6): 52-58. DOI: 10.12131/20180100
Citation: WANG Wenhao, DONG Hongbiao, SUN Yongxu, CAO Ming, DUAN Yafei, LI Hua, LIU Qingsong, ZHANG Jiasong. Anesthetic effects of MS-222 and eugenol in simulated transportation of juvenile largemouth bass (Micropterus salmoides)[J]. South China Fisheries Science, 2018, 14(6): 52-58. DOI: 10.12131/20180100

Anesthetic effects of MS-222 and eugenol in simulated transportation of juvenile largemouth bass (Micropterus salmoides)

More Information
  • Received Date: May 14, 2018
  • Revised Date: June 06, 2018
  • Accepted Date: June 25, 2018
  • Available Online: December 05, 2018
  • To determine the appropriate anesthetic method and dose for juvenile Micropterus salmoides in laboratory, we compared the hydrostatic anesthesia effects of two common fish anesthetics (MS-222 and eugenol) on the juveniles with different concentrations (40 mg·L–1, 50 mg·L–1, 60 mg·L–1, 70 mg·L–1, 80 mg·L–1, 90 mg·L–1, 100 mg·L–1 and 8 mg·L–1, 10 mg·L–1, 12 mg·L–1, 14 mg·L–1, 16 mg·L–1, 18 mg·L–1, 20 mg·L–1). The results show: 1. With increasing concentrations of the two anesthetics, the anesthetic time shortened but the recovery time prolonged. 2. The optimal anesthetic concentrations of MS-222 and eugenol were 70 mg·L–1 and 16 mg·L–1, respectively, while the optimal anesthetic transportation concentrations of the two anesthetics were 50 mg·L–1 and 10 mg·L–1, respectively. Then we carried out an experiment of 10-hour anesthesia transport under the optimal anesthetic concentrations of MS-222 and eugenol , and the survival rate during the transportation and after 24 h of recovery for the anesthesia group were both 100%, significantly higher than those for the non-anesthetic transport group (P<0.05). The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels increased significantly in the anesthesia transport group compared with those in the non-anesthetic transport group and hydrostatic control group (P<0.05). The ALT and AST levels in the MS-222 anesthesia group were significantly higher than those in the eugenol anesthesia group (P<0.05).
  • [1]
    魏涯, 钱茜茜, 吴燕燕, 等. 栅栏技术在淡腌半干鲈鱼加工工艺中的应用[J]. 南方水产科学, 2017, 13(2): 109-120.
    [2]
    刘长琳, 何力, 陈四清, 等. 鱼类麻醉研究综述[J]. 渔业现代化, 2007, 34(5): 21-25.
    [3]
    BOLASINA S N, AZEVEDO A D, PETRY A C. Comparative efficacy of benzocaine, tricaine methanesulfonate and eugenol as anesthetic agents in the guppy Poecilia vivipara[J]. Aquacult Rep, 2017, 6: 56-60.
    [4]
    PATTANASIRI T, TAPARHUDEE W, SUPPAKUL P. Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish, Betta splendens[J]. Aquacult Int, 2017, 25(1): 163-175.
    [5]
    严银龙, 施永海, 张海明, 等. MS-222、丁香酚对刀鲚幼鱼的麻醉效果[J]. 上海海洋大学学报, 2016, 25(2): 177-182.
    [6]
    MITJANA O, BONASTRE C, TEJEDOR M T, et al. Simultaneous effect of sex and dose on efficacy of clove oil, tricaine methanesulfonate, 2-phenoxyethanol and propofol as anaesthetics in guppies, Poecilia reticulata (Peters)[J]. Aquac Res, 2018, 49(6): 2140-2146.
    [7]
    刘莹, 于超勇, 官曙光, 等. MS-222麻醉黑鲷幼鱼的初步研究[J]. 齐鲁渔业, 2015(10): 1-4.
    [8]
    胡发文, 李莉, 刘元文, 等. 丁香油和MS-222对大泷六线鱼幼鱼的麻醉效果[J]. 大连海洋大学学报, 2017, 32(6): 671-675.
    [9]
    王利娟, 程守坤, 张饮江, 等. MS-222在加州鲈鱼模拟运输中的麻醉效果[J]. 上海海洋大学学报, 2015, 24(2): 235-241.
    [10]
    COOKE S J, SUSKI C D, OSTRAND K G, et al. Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass (Micropterus salmoides)[J]. Aquaculture, 2004, 239(1/2/3/4): 509-529.
    [11]
    BARBAS L, PEREIRA-CARDONA P M, MALTEZ L, et al. Anaesthesia and transport of juvenile tambaqui Colossoma macropomum (Cuvier, 1818) with tricaine methane-sulphonate: implications on secondary and oxidative stress responses[J]. J Appl Ichthyol, 2017, 33(4): 720-730.
    [12]
    BECKER A G, LUZ R K, MATTIOLI C C, et al. Can the essential oil of Aloysia triphylla have anesthetic effect and improve the physiological parameters of the carnivorous freshwater catfish Lophiosilurus alexandri after transport?[J]. Aquaculture, 2017, 481: 184-190.
    [13]
    HIKASA Y, TAKASE K, OGASAWARA T, et al. Anesthesia and recovery with tricaine methanesulfonate, eugenol and thiopental sodium in the carp, Cyprinus carpio[J]. Nihon Juigaku Zasshi, 1986, 48(2): 341-351.
    [14]
    MCFARLAND W N, KLONTZ G W. Anesthesia in fishes[J]. Fed Proc, 1969, 28(4): 1535-1540.
    [15]
    BALAMURUGAN J, KUMAR T T, MEENAKUMARI B, et al. Clove extract: a potential source for stress free transport of fish[J]. Aquaculture, 2016, 454: 171-175.
    [16]
    KIESSLING A, JOHANSSON D, ZAHL I H. Pharmacokinetics, plasma cortisol and effectiveness of benzocaine, MS-222 and isoeugenol measured in individual dorsal aorta-cannulated Atlantic salmon (Salmo salar) following bath administration[J]. Aquaculture, 2009, 286(3/4): 301-308.
    [17]
    ZAHL I H, KIESSLING A, SAMUELSEN O B. Anaesthesia of Atlantic halibut (Hippoglossus hippoglossus): effect of pre-anaesthetic sedation, and importance of body weight and water temperature[J]. Aquacult Res, 2011, 42(9): 1235-1245.
    [18]
    ZAHL I H, KIESSLING A, SAMUELSEN O B. Anaesthesia of Atlantic cod (Gadus morhua): effect of pre-anaesthetic sedation, and importance of body weight, temperature and stress[J]. Aquaculture, 2009, 295(1/2): 52-59.
    [19]
    MYLONAS C C, CARDINALETTI G, SIGELAKI I, et al. Comparative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures[J]. Aquaculture, 2005, 246(1/2/3/4): 467-481.
    [20]
    National Toxicology Program. NTP toxicology and carcinogenesis studies of methyleugenol (CAS NO. 93-15-2) in F344/N rats and B6C3F1 mice (Gavage Studies)[J]. Natl Toxicol Program Tech Rep Se, 2000, 491: 1-412.
    [21]
    HILL J V, FORSTER M E. Cardiovascular responses of Chinook salmon (Oncorhynchus tshawytscha) during rapid anaesthetic induction and recovery[J]. Comp Biochem Physiol C, 2004, 137(2): 167-177.
    [22]
    MYSZKOWSKI L, KAMIŃSKI R, WOLNICKI J. Response of juvenile tench Tinca tinca (L.) to the anaesthetic 2-phenoxyethanol[J]. J Appl Ichthyol, 2003, 19(3): 142-145.
    [23]
    KUMLU M, YANAR M. Effects of the anesthetic quinaldine sulphate and muscle relaxant diazepam on sea bream juveniles (Sparus aurata)[J]. Isr J Aquacult-Bamid, 1999, 51(4): 143-147.
    [24]
    CROSBY T C, HILL J E, WATSON C A, et al. Effects of tricaine methanesulfonate, hypno, metomidate, quinaldine, and salt on plasma cortisol levels following acute stress in threespot gourami Trichogaster trichopterus[J]. J Aquat Anim Health, 2006, 18(1): 58-63.
    [25]
    段亚飞, 董宏标, 王芸, 等. 干露胁迫对日本囊对虾抗氧化酶活性的影响[J]. 南方水产科学, 2015, 11(4): 102-108.
    [26]
    孙金辉, 范泽, 张美静, 等. 饲料蛋白水平对鲤幼鱼肝功能和抗氧化能力的影响[J]. 南方水产科学, 2017, 13(3): 113-119.
    [27]
    陈劲松, 江世贵, 黄建华, 等. 斑节对虾天门冬氨酸转氨酶基因的克隆及氨氮胁迫条件下的表达分析[J]. 南方水产科学, 2017, 13(3): 73-82.
    [28]
    FENG G P, ZHUANG P, ZHANG L Z, et al. Effects of water temperature on metabolic enzyme and antioxidase activities in juvenile Chinese sturgeon (Acipenser sinensis)[J]. Acta Pharmacol Sin, 2012, 93(36): 137-142.
    [29]
    ANDERSON F H, ZENG L, ROCK N R, et al. An assessment of the clinical utility of serum ALT and AST in chronic hepatitis C[J]. Hepatol Res, 2000, 18(1): 63-71.
    [30]
    王吉桥, 徐锟. 对虾的主要疾病及其诊断方法[J]. 水产科学, 2002, 21(5): 23-28.
    [31]
    廖金花, 陈巧, 林丽蓉, 等. 鲍鱼碱性磷酸酶的分离纯化和性质研究[J]. 厦门大学学报(自然科学版), 2005, 44(2): 272-275.
    [32]
    张丽, 汪之和. MS-222对大黄鱼成鱼麻醉效果的研究[J]. 科技与产业, 2010(18): 38-40.
    [33]
    赵艳丽, 杨先乐, 黄艳平, 等. 丁香酚对大黄鱼麻醉效果的研究[J]. 水产科技情报, 2002, 29(4): 163-165.
    [34]
    朱金段, 袁德俊, 林新颖. 丁香的药理研究现状及临床应用[J]. 中国药物经济学, 2013(1): 32-35.
    [35]
    刘双凤, 蔡勋. 鱼用麻醉剂的研究进展[J]. 黑龙江水产, 2008(6): 40-43.
    [36]
    PAWAR H B, SANAYE S V, SREEPADA R A, et al. Comparative efficacy of four anaesthetic agents in the yellow seahorse, Hippocampus kuda (Bleeker, 1852)[J]. Aquaculture, 2011, 311(1/2/3/4): 155-161.
    [37]
    HOSKONEN P, PIRHONEN J. The effect of clove oil sedation on oxygen consumption of six temperate-zone fish species[J]. Aquacult Res, 2004, 35(10): 1002-1005.
    [38]
    WELKER T L, LIM C, YILDIRIMAKSOY M, et al. Effect of buffered and unbuffered tricaine methanesulfonate (MS-222) at different concentrations on the stress responses of channel catfish, Ictalurus punctatus Rafinesque[J]. J Appl Aquacult, 2007, 19(3): 1-18.
  • Related Articles

    [1]LIN Liqun, ZHANG Yaoming, LIU Ping, XU Zhiqiang, CUI Mingchao. Influencing factors of gas-liquid two-phase flow in oxygenation speece cone of aquaculture tanks[J]. South China Fisheries Science, 2025, 21(3): 36-43. DOI: 10.12131/20240224
    [2]JIANG Manju, GUO Yu, QIN Chuanxin, XIN Yi, ZHAO Xinran, YU Gang, MA Zhenhua, YANG Yukai. Investigation on behavioral preferences of Lutjanus erythropterus juvenile towards artificial reef models with different pore shapes and sizes[J]. South China Fisheries Science, 2024, 20(5): 42-52. DOI: 10.12131/20240135
    [3]HUANG Li, GAO Lei, WU Song, HAO Qirui, LI Chenhui, TANG Shizhan, BAI Shuyan, CHEN Zhongxiang, DU Ningning, QIN Dongli, WANG Peng. Variation and accumulation characteristics of diazepam in simulated culture environment[J]. South China Fisheries Science, 2024, 20(2): 38-47. DOI: 10.12131/20230128
    [4]HONG Yongqiang,  XIE Yonghe,  LIU Luqiang,  DONG Shaoguang,  LI Detang,  WANG Yunjie,  JIANG Xuyang,  ZHANG Jiaqi,  WANG Jun,  GAO Weipeng,  CHEN Qing. Research on water quality dissolved oxygen prediction method based on ESSA-LSTM for aquaculture ships[J]. South China Fisheries Science, 2024, 20(1): 62-73. DOI: 10.12131/20230185
    [5]ZHANG Jiaqi, XIE Yonghe, LI Detang, GAO Weipeng, CHEN Qing, WANG Jun, WANG Yunjie, HONG Yongqiang. Structural design and research of underwater robot for aquaculture ship operation[J]. South China Fisheries Science, 2024, 20(1): 11-24. DOI: 10.12131/20230192
    [6]XING Wang, HUANG Xiaohua, LI Gen, PANG Guoliang, YUAN Taiping. Construction of a dynamics simulation system for net cleaning robot based on Gazebo[J]. South China Fisheries Science, 2024, 20(1): 1-10. DOI: 10.12131/20230189
    [7]SU Huifeng, DING Lesheng, WANG Xuwang, CHEN Musheng, CHEN Xiao. Prediction of dissolved oxygen in water of aquaculture ship based on CNN-GRU hybrid model[J]. South China Fisheries Science, 2023, 19(4): 174-180. DOI: 10.12131/20220298
    [8]HU Yu, HUANG Xiaohua, TAO Qiyou, YUAN Taiping, WANG Shaomin, LIU Haiyang. Simulation of automatic pneumatic conveying process of automatic feeding system based on computational fluid dynamics-discrete element method[J]. South China Fisheries Science, 2019, 15(3): 113-119. DOI: 10.12131/20180219
    [9]HU Yu, GUO Genxi, HUANG Xiaohua, TAO Qiyou, ZHANG Xiaoming. Simulation of flow field inside the net cleaning machine based on ANSYS[J]. South China Fisheries Science, 2010, 6(1): 7-11. DOI: 10.3969/j.issn.1673-2227.2010.01.002
    [10]LI Min, ZHU Changbo, ZHANG Hanhua, CHEN Lixiong, WU Jinfeng. Effects of diets acclimation on the feeding preference of juvenile black abalone[J]. South China Fisheries Science, 2007, 3(4): 14-19.
  • Cited by

    Periodical cited type(4)

    1. 刘鸿,米乃瓦尔·木衣提,胡江伟,贺疆滔,张涛,杨博文,牛建功. 不同荧光标记物对扁吻鱼标记效果的对比观察. 黑龙江水产. 2025(03): 243-253 .
    2. 王纪鹏,虢有婕,韩毅,张龙涛,缪松,郑宝东. pH敏感性材料在智能食品包装中的应用进展. 食品工业科技. 2023(01): 48-55 .
    3. 祝超文,张虎,贲成恺,袁健美,胡海生,肖悦悦. 江苏近岸海域大黄鱼标记放流及效果评估. 水产养殖. 2023(06): 1-9 .
    4. 冯瑞玉,郭禹,李金明,孙金辉,于刚,吴一桂,秦传新. 基于EnhanceFish模型的鱼类增殖放流策略研究:以中山市南朗水域黄鳍棘鲷增殖放流为例. 渔业科学进展. 2023(05): 1-10 .

    Other cited types(3)

Catalog

    Recommendations
    Water quality evaluation foreriocheir sinensisaquaculture ponds with different aquatic plants
    HUA Qinghong et al., SOUTH CHINA FISHERIES SCIENCE, 2025
    Progress in study and application of fish bioenergetics models
    ZHANG Xianbing et al., SOUTH CHINA FISHERIES SCIENCE, 2024
    Research on fish feeding intensity classification model based on axial feature calibration and temporal segment network
    XU Bo et al., SOUTH CHINA FISHERIES SCIENCE, 2024
    Study on diurnal and nocturnal variation of fish resources in marine ranching by fixed-point monitoring based on acoustic technology
    CHENG Gao et al., SOUTH CHINA FISHERIES SCIENCE, 2024
    The system establishment of improved seed, healthy aquaculture and high-quality sales of fish
    WANG Shi et al., JOURNAL OF FISHERIES OF CHINA, 2023
    Flow velocity on fish and its application in recirculating aquaculture system
    ZHU Ting-Yao et al., ACTA HYDROBIOLOGICA SINICA, 2025
    The contribution of aquaculture systems to global aquaculture production
    Verdegem, Marc et al., JOURNAL OF THE WORLD AQUACULTURE SOCIETY, 2023
    Semantic-preserved communication system for highly efficient speech transmission
    Han, Tianxiao et al., IEEE JOURNAL ON SELECTED AREAS IN COMMUNICATIONS, 2023
    Ship formation and route optimization design based on improved pso and d-p algorithm
    IEEE ACCESS, 2025
    A novel optimization framework for efficiently identifying high-quality pareto-optimal solutions: maximizing resilience of water distribution systems under cost constraints
    RELIABILITY ENGINEERING & SYSTEM SAFETY
    Powered by
    Article views (4019) PDF downloads (51) Cited by(7)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return