Volume 17 Issue 4
Aug.  2021
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LI Junwei, HU Ruiping, CHEN Suwen, GUO Yongjian, ZHU Changbo, LI Ting, XIE Xiaoyong, SU Jiaqi. Effects of low salinity pressure on biological tissue and immunity enzymes activities of Sipunculus nudus[J]. South China Fisheries Science, 2021, 17(4): 41-48. DOI: 10.12131/20210022
Citation: LI Junwei, HU Ruiping, CHEN Suwen, GUO Yongjian, ZHU Changbo, LI Ting, XIE Xiaoyong, SU Jiaqi. Effects of low salinity pressure on biological tissue and immunity enzymes activities of Sipunculus nudus[J]. South China Fisheries Science, 2021, 17(4): 41-48. DOI: 10.12131/20210022
shu

Effects of low salinity pressure on biological tissue and immunity enzymes activities of Sipunculus nudus

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China

  • 2.

    Haiwei Aquaculture Technology, Co., Ltd., Guangzhou 510300, China

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  • Received Date: January 12, 2021
  • Revised Date: March 18, 2021
  • Accepted Date: April 06, 2021
  • Available Online: April 13, 2021
    Graphical Abstract
    • Abstract
  • In order to understand the response of the biological and physiological indicators of Sipunculus nudus to low salinity, we evaluated the effects of low salinity pressure on the survival rate, osmotic pressure, biological tissue and immunity enzymes activities of S. nudus which were cultured for 96 h in water with abrupt salinity drop of 0 (S30), 5 (S25), 10 (S20), 15 (S15), 20 (S10) and 25 (S5). The results show that: 1) S. nudus could tolerate the salinity drop of 10, but would die when the salinity dropped over 15. 2) The coelomic fluid of S. nudus increased with the decrease of water salinity significantly, and there was a significant positive correlation between the body mass and coelomic fluid volumn (R2>0.96). 3) The osmotic pressure and body protein contents decreased with the decrease of water salinity (P<0.05). 4) In the low salinity treatments, the connective tissue of longitudinal and circumferential muscle fibers of the body wall were thin, and the staining of columnar cells was lighter. For the tentacle, its adhesion ability was weaker as the epithelial cells became larger. 5) SOD and CAT activities in the groups of S30 and S25 were relatively stable, while the body SOD and coelomic fluid CAT in the groups of S10 and S15 first decreased and then increased; however, the CAT of coelomic fluid in the groups of S10 and S15 first increased and then decreased. SOD and CAT activities in the coelomic fluid had strong varation. In summary, low salinity pressure can affect the survival rate, osmotic pressure, biological tissue and immunity enzymes activities of S. nudus significantly.
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