Volume 17 Issue 6
Dec.  2021
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LUO Yuting, FANG Di'an, ZHOU Yanfeng, XU Dongpo, PENG Yunxin, PENG Fei, ZHANG Guining, LIU Kai, YOU Yang. Genetic diversity of silver carp (Hypophthalmichthys molitrix) in lower reaches of Yangtze River based on microsatellite markers[J]. South China Fisheries Science, 2021, 17(6): 48-57. DOI: 10.12131/20210067
Citation: LUO Yuting, FANG Di'an, ZHOU Yanfeng, XU Dongpo, PENG Yunxin, PENG Fei, ZHANG Guining, LIU Kai, YOU Yang. Genetic diversity of silver carp (Hypophthalmichthys molitrix) in lower reaches of Yangtze River based on microsatellite markers[J]. South China Fisheries Science, 2021, 17(6): 48-57. DOI: 10.12131/20210067
shu

Genetic diversity of silver carp (Hypophthalmichthys molitrix) in lower reaches of Yangtze River based on microsatellite markers

  • 1.

    Wuxi Fisheries College, Nanjing Agriculture University, Wuxi 214081, China

  • 2.

    Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

  • 3.

    Shanghai Ocean University/National Demonstration Center for Experimental Fisheries Science Education, Shanghai 201306, China

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  • Received Date: February 28, 2021
  • Revised Date: April 13, 2021
  • Accepted Date: May 05, 2021
  • Available Online: May 28, 2021
    Graphical Abstract
    • Abstract
  • In order to understand the genetic diversity and distribution of silver carp (Hypophthalmichthys molitrix) in the lower reaches of the Yangtze River, we used simple sequence repeats (SSR) combining with capillary electrophoresis to analyze 327 DNA samples of silver carp from eight wild populations in Yangtze River sections (Hukou, Anqing, Wuhu, Dangtu, Zhenjiang, Jingjiang, Zhangjiagang and Changshu sections) from 2017 to 2019, so as to investigate their genetic diversity, genetic differentiation and genetic structure. The results show that the genetic diversity of the eight populations was high. However, heterozygotes were not enough in the populations. The number of alleles ranged from 6.00 to 12.3, while the number of effective alleles ranged from 3.94 to 6.10. The observed heterozygosity and expected heterozygosity ranged from 0.617 to 0.719 and from 0.658 to 0.774, respectively. The unbiased expected heterozygosity ranged from 0.671 to 0.782. The Shannon's information index ranged from 1.38 to 1.86, and the inbreeding coefficient ranged from 0.001 to 0.174. The genetic differentiation analysis shows that the Fst value ranged from 0.006 to 0.068, while the gene flow ranged from 3.41 to 41.9. The genetic distance ranged from 0.001 to 0.106. Hukou population showed a moderate degree of genetic difference with the other populations, while the genetic differences among the other populations were small. Analysis of molecular variance shows that the variation mainly was within the population (97.6%). Genetic structure analysis shows that Hukou population had different gene pool from the other populations. The river-lake connectivity may be of positive significance for silver carp to show unique genetic differentiation characteristics in Hukou section of the Yangtze River. Therefore, whether the formation of this population differentiation is caused by the spatial isolation of geographical distance or the physiological barrier of habitat difference needs to be further studied.
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