WU Lan, XIE Jun, WANG Guangjun, YU Deguang, HU Chaoying, NIU Jifeng. Effect of the metalloprotease on growth performance, digestibility and non-specific immune of hybrid tilapia Oreochromis niloticus × O.aureus[J]. South China Fisheries Science, 2007, 3(3): 8-13.
Citation: WU Lan, XIE Jun, WANG Guangjun, YU Deguang, HU Chaoying, NIU Jifeng. Effect of the metalloprotease on growth performance, digestibility and non-specific immune of hybrid tilapia Oreochromis niloticus × O.aureus[J]. South China Fisheries Science, 2007, 3(3): 8-13.

Effect of the metalloprotease on growth performance, digestibility and non-specific immune of hybrid tilapia Oreochromis niloticus × O.aureus

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  • Received Date: December 21, 2006
  • Revised Date: January 14, 2007
  • The effects of adding different level of metalloprotease in the diet on growth performance, apparent digestibility and non-specific immune of tilapia (Oreochromis niloticus × O.aureus)were investigated. Four different diets were fed to fish for 56 days: a basic diet as control and three diets supplemented with 0.03%, 0.05% and 0.10% metalloprotease. Fish fed with the diet added 0.10% enzyme had significantly higher weight gain and lower feed conversion ratio (FCR) than that of the control group (P < 0.05).Furthermore, the addition of the 0.10% metalloprotease had positive effects on the protein apparent digestibility (P < 0.05) and dry matter apparent digestibility (P < 0.05), and the level of 0.05% also improved the dry matter apparent digestibility significantly (P < 0.05).Superoxide dismutase (SOD) activity in tilapia serum was significantly higher than that of the control group when the fish fed with the diet supplemented with 0.10% metalloprotease.In conclusion, the addition of dietary metalloprotease had significant positive effects on growth performance, protein digestibility and nutrient utilization of tilapia, and enhanced the activities of SOD in tilapia serum.

  • [1]
    李孝辉. 饲用微生物酶制剂及其研究应用概况[J]. 饲料工业, 2001, 22(1): 40-43.
    [2]
    王新梅, 朱文渊. 饲用酶制剂发展概况[J]. 新疆农业科学, 2004, 41(专刊): 112-115. doi: 10.3969/j.issn.1001-4330.2004.z1.041
    [3]
    PARK M J, SHIN D H, SON K H, et al. The effect of the addition of a protease Arazyme Ron the dissolution of proteins in animal and vegetable feed materials[R]. Daejeon: Insect Resources Laboratory, Korea Research Institute of Bioscience and Biotechnology, R & D Center, Insect Biotech Co., Ltd, 2004.
    [4]
    PARK M J, PARK D S, SHIN D H, et al. The effect of consecutive treatment by phytase and protease, Arazyme on the protein degrada-tion in soybean meal[R]. Daejeon: Insect Resources Laboratory, Korea Research Institute of Bioscience and Biotechnology, R & D Center, Insect Biotech Co., Ltd, 2004.
    [5]
    姚瑞清. 植酸酶对奥尼罗非鱼营养物质表观消化率及消化酶活性的影响[D]. 南京: 南京农业大学, 2005. 10.7666/d.Y749076
    [6]
    施培松. 复合酶制剂对草鱼鱼种生长性能及消化酶的影响[D]. 武汉: 华中农业大学, 2005. 10.7666/d.Y806445
    [7]
    吴天星, 王亚军. 日量中添加甲酸钙和酸性蛋白酶对断奶仔猪生产性能的影响[J]. 中国畜牧杂志, 2002, 38(2): 23-25. doi: 10.3969/j.issn.0258-7033.2002.02.010
    [8]
    GHAZI S, ROOKE T A, GALBRATH H, et al. The potential for improving soya-bean meal in diets for chickens: treated with differ-ent proteolytic enzyme[J]. British Poultry Sci, 1997b, 37(2): 554-555.
    [9]
    钟国防, 周洪琪. 木聚糖酶和复合酶制剂PS对尼罗罗非鱼生长性能、消化率及肌肉营养成分的影响[J]. 浙江海洋学院学报, 2005, 24(4): 324-329. doi: 10.3969/j.issn.1008-830X.2005.04.005
    [10]
    王爱民, 刘文斌. 外源酶对异育银鲫生长及表观消化率的影响研究[J]. 饲料工业, 2006, 27(2): 26-29. doi: 10.3969/j.issn.1001-991X.2006.02.008
    [11]
    韩庆, 夏维福, 罗玉双, 等. 酶制剂对黄颡鱼生长性能的影响[J]. 水产学杂志, 2002, 15(1): 84-87. doi: 10.3969/j.issn.1005-3832.2002.01.019
    [12]
    陈应华. 复合酶制剂对彭泽鲫生长性能的影响试验[J]. 饲料工业, 2001, 22(3): 27-28. doi: 10.3969/j.issn.1001-991X.2001.03.010
    [13]
    HEO G J. A study on efficacy of the mixed digestive in cultured fish, Cyprinus carpio and Oncohyncus mykiss[J]. Korean J Lab Anim Sci, 1996, 12(3): 31-36.
    [14]
    [15]
    MISRA C K, DAS B K, MUKHERJEE S C, et al. Effect of multi-ple injections of β-glucan on non-specific immune response and disease resistance in Labeo rohita fingerlings[J]. Fish & Shellf Immunol, 2006, 20(3): 305-319. doi: 10.1016/j.fsi.2005.05.007
    [16]
    何四旺, 许国焕, 吴月嫦, 等. 低聚异麦芽糖和低聚果糖对罗非鱼生长和非特异性免疫的影响[J]. 中国饲料, 2003(23): 14-15. doi: 10.3969/j.issn.1004-3314.2003.23.008
    [17]
    [18]
    李桂峰, 钱沛锋, 孙际佳, 等. 维生素C对胡子鲶血清免疫相关酶活性的影响[J]. 大连水产学院学报, 2004, 19(4): 301-305. doi: 10.3969/j.issn.1000-9957.2004.04.014
    [19]
    乔高华, 刘至治, 冷向军. 饲料中添加β-葡聚糖和低聚果糖对中华鳖幼鳖生长和血清SOD、溶菌酶活力的影响[J]. 上海水产大学学报, 2004, 13(1): 36-41. doi: 10.3969/j.issn.1004-7271.2004.01.007
    [20]
    熊沈学. 饲料中添加低聚木糖对异育银鲫增重率、消化酶活性及免疫功能的影响[D]. 南京: 南京农业大学, 2005. 10.7666/d.Y748884
    [21]
    王吉桥, 孙永新, 张剑诚. 金银花等复方中草药对牙鲆生长、消化和免疫功能的影响[J]. 水产学报, 2006, 30(1): 90-96. http://scxuebao.ijournals.cn/scxuebao/article/abstract/20060114
    [22]
    谢一荣, 吴锐全, 谢骏, 等. 维生素C对大口黑鲈生长和非特异性免疫功能的影响[J]. 南方水产, 2006, 2(3): 40-45. doi: 10.3969/j.issn.2095-0780.2006.03.006
    [23]
    LEE M H, SHIAU S Y. Vitamin E requirements of juvenile grass shrimp, Penaeus monodon, and effects on non-specific immune re-sponses[J]. Fish & Shellf Immunol, 2004, 16(4): 475-485. doi: 10.1016/j.fsi.2003.08.005
    [24]
    钟国防, 周洪琪. 木聚糖酶和复合酶制剂PS对尼罗罗非鱼生长性能、非特异性免疫能力的影响[J]. 海洋渔业, 2005, 27(4): 286-291. doi: 10.3969/j.issn.1008-830X.2005.04.005

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