Volume 18 Issue 6
Dec.  2022
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YUAN Zhongjin, CEN Jianwei, LI Laihao, YANG Xianqing, HUANG Hui, WEI Ya, HAO Shuxian, ZHAO Yongqiang, WANG Yueqi, LIN Zhi. Effect of low-temperature acclimation on survival, non-specific immune and antioxidant indexes of Epinephelus fuscoguttatus ♀×E. lanceolatus ♂[J]. South China Fisheries Science, 2022, 18(6): 118-126. DOI: 10.12131/20220042
Citation: YUAN Zhongjin, CEN Jianwei, LI Laihao, YANG Xianqing, HUANG Hui, WEI Ya, HAO Shuxian, ZHAO Yongqiang, WANG Yueqi, LIN Zhi. Effect of low-temperature acclimation on survival, non-specific immune and antioxidant indexes of Epinephelus fuscoguttatus ♀×E. lanceolatus ♂[J]. South China Fisheries Science, 2022, 18(6): 118-126. DOI: 10.12131/20220042
shu

Effect of low-temperature acclimation on survival, non-specific immune and antioxidant indexes of Epinephelus fuscoguttatus ♀×E. lanceolatus ♂

  • 1.

    College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Processing, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 3.

    Dalian Polytechnic University/Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China

  • 4.

    Guangdong Shunxin Ocean Fishery Group Co. Ltd., Yangjiang 529800, China

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  • Received Date: February 23, 2022
  • Revised Date: March 23, 2022
  • Accepted Date: April 04, 2022
  • Available Online: April 15, 2022
    Graphical Abstract
    • Abstract
  • Investigating the best acclimation temperature conditions for Epinephelus fuscoguttatus ♀×E. lanceolatus ♂ can provide a theoretical basis for its survival and circulation. We decreased the water temperature to 24, 20, 18 and 16 ℃ at the rate of 0.5−1.0 ℃·h−1, then recorded the physiological responses and survival time, as well as the changes of water quality, non-specific immune index and antioxidant indicator. The results show that the survival time of the grouper increased with the decrease of temperature within the range of 24−16 ℃. When the water temperature was maintained at 16 ℃, the concentration of total ammonia nitrogen (TAN) increased slowly, and the survival time was the longest, up to 109 h. The non-specific immune and antioxidant parameters changed after the grouper being cultured at different temperatures for 48 h. Compared with the control group, the contents of complement protein 3 (C3), globulin (GLB) and total protein (TP) increased in 16 ℃ group significantly, while the other indexes had no significant changes. The activities of superoxide dismutase (SOD) and catalase (CAT) in the liver tissue in 16 ℃ group increased significantly. However, the activity of glutathione peroxidase (GPx) decreased significantly, but higher than those of the other temperature groups. Compared with the other temperature treated groups, the content of malondialdehyde (MDA) decreased significantly. Thus, 16 ℃ temporary culture can not only maintain good water quality, but also reduce the metabolic level of fish and maintain the homeostasis of internal environment, so as to reduce the damage caused by oxidative stress reaction. It is suitable for temporary culture and transportation of Epinephelus fuscoguttatus ♀×E. lanceolatus ♂.
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