Volume 3 Issue 5
Oct.  2007
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LI Lihao, YU Dahui, HUANG Guiju, DU Bo, FU Yun, TONG Xin, GUO Yihui, YE Wei. Genetic diversity of selected GIFT strains of Oreochromis niloticus[J]. South China Fisheries Science, 2007, 3(5): 40-48.
Citation: LI Lihao, YU Dahui, HUANG Guiju, DU Bo, FU Yun, TONG Xin, GUO Yihui, YE Wei. Genetic diversity of selected GIFT strains of Oreochromis niloticus[J]. South China Fisheries Science, 2007, 3(5): 40-48.
shu

Genetic diversity of selected GIFT strains of Oreochromis niloticus

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Shanghai Fisheries University, Shanghai 200090, China

  • 3.

    Fisheries College, Huazhong Agricultural University, Wuhan 430070, China

  • 4.

    Guangdong National Elite Tilapia Farm, Guangzhou 511453, China

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  • Received Date: May 24, 2007
  • Revised Date: June 19, 2007
    Graphical Abstract
    • Abstract

  • Genetic diversity and differentiation among three stocks of selected GIFT strains of Oreochromis niloticus was analyzed using microsatellite DNA and AFLP markers. Nine microsatellite loci and four primer pairs of AFLP revealed consistent results. Microsatellite data showed that Qingdao GIFT stock had the highest mean number of alleles (4.8), mean observed heterozygosity (0.528) and mean PIC (0.605), whereas Hainan GIFT the lowest. AFLP analysis demonstrated that Qingdao GIFT had the highest proportion of polymorphic loci (48.4%) and gene diversity within populations (0.245), whereas Hainan GIFT the lowest. These observations indicated that Qingdao GIFT has the highest genetic diversity, whereas Hainan GIFT the lowest. Genetic differentiation among the three GIFT strains was significant as revealed by microsatellite (FST=0.07~0.11, P < 0.01) and AFLP (FST =0.24~0.29, P < 0.01).AMOVA indicated that the majority of genetic variation (91.26% by microsatellite analysis and 67.6% by AFLP) was attributed to difference of individuals within stocks, suggesting that GIFT strains of O.niloticus has some potential for further selective breeding.

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    • References (26)
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