Volume 15 Issue 4
Aug.  2019
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ZHAO Yanhua, OU Youjun, WEN Jiufu, LI Jia'er, ZHOU Hui. Analysis of genetic diversity of Bahaba flavolabiata based on microsatellite markers[J]. South China Fisheries Science, 2019, 15(4): 127-132. DOI: 10.12131/20180261
Citation: ZHAO Yanhua, OU Youjun, WEN Jiufu, LI Jia'er, ZHOU Hui. Analysis of genetic diversity of Bahaba flavolabiata based on microsatellite markers[J]. South China Fisheries Science, 2019, 15(4): 127-132. DOI: 10.12131/20180261
shu

Analysis of genetic diversity of Bahaba flavolabiata based on microsatellite markers

  • 1.

    Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    National Demonstration Center for Experimental Fisheries Science Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

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  • Received Date: November 25, 2018
  • Revised Date: March 26, 2019
  • Accepted Date: April 25, 2019
  • Available Online: May 06, 2019
    Graphical Abstract
    • Abstract

  • We examined the genetic diversity of 19 Bahaba flavolabiata samples collected from the Pearl River estuary based on microsatellite markers, so as to provide references for the identification of its germplasm resources and future breeding. The results show that the number of alleles (Na) in each of the 29 microsatellite loci was 2−7, with a total number of 121 alleles. The average number of alleles in each locus was 4.17. The effective number of alleles (Ne) was 1.060 6−6.056 1, with a sum of 85.205 0. The average values of the observed heterozygosity (Ho) and the expected heterozygosity (He) were 0.659 3 and 0.597 3, respectively. The value of polymorphism information content (PIC) was 0.055 5−0.813 7, with an average of 0.542 3, and 19 loci showed high polymorphism. Five loci deviated significantly from the Hardy-Weinberg equilibrium (corrected P≤0.036). The allelic richness (Rs) was 2−6.976, with a sum of 119.638. The genetic diversity (GD) was 0.059−0.856. It is suggested that most of the SSR loci developed in this study are highly polymorphic, and the genetic diversity of B. flavolabiata population is at a higher level.

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  • [1]
    朱元鼎, 罗云林, 伍汉霖. 中国鱼类专著集[M]. 上海: 上海科学技术出版社, 1991: 40-41.
    [2]
    区又君, 廖锐, 李加儿, 等. 野生黄唇鱼Bahaba flavolabiata肌肉和鳔营养成分的分析与评价[J]. 海洋学报(中文版), 2010, 32(3): 113-120.
    [3]
    BABAEI H, ZEINALIAN M, EMAMI M H, et al. Simplified microsatellite instability detection protocol provides equivalent sensitivity to robust detection strategies in Lynch syndrome patients[J]. Cancer Biol Med, 2017, 14(2): 142-150. doi: 10.20892/j.issn.2095-3941.2016.0091
    [4]
    YAMAMOTO H, ADACHI Y, TANIGUCHI H, et al. Interrelationship between microsatellite instability and microRNA in gastrointestinal cancer[J]. World J Gastroenterol, 2012(22): 2745-2755.
    [5]
    孙成飞, 叶星, 董浚键, 等. 罗氏沼虾6个养殖群体遗传多样性的微卫星分析[J]. 南方水产科学, 2015, 11(2): 20-26. doi: 10.3969/j.issn.2095-0780.2015.02.003
    [6]
    杨兵, 林琳, 李纯厚, 等. 基于高通量测序的二长棘鲷微卫星标记开发与评价[J]. 南方水产科学, 2015, 11(4): 116-120. doi: 10.3969/j.issn.2095-0780.2015.04.017
    [7]
    孔杰, 石婧, 向燕, 等. 基于微卫星标记的养殖施氏鲟亲鱼群体遗传多样性[J]. 贵州农业科学, 2015, 43(12): 119-122. doi: 10.3969/j.issn.1001-3601.2015.12.031
    [8]
    袁文成. 基于转录组测序的翘嘴鳜微卫星标记的开发及MHC classI基因的克隆表达[D]. 苏州: 苏州大学, 2015: 12-28.
    [9]
    龚诗琦, 王志勇, 肖世俊, 等. 黄姑鱼转录组SSR的开发与验证[J]. 集美大学学报(自然科学版), 2016, 21(4): 241-246. doi: 10.3969/j.issn.1007-7405.2016.04.001
    [10]
    EXCOFFIER L, LISCHER H E. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows[J]. Mol Ecol Resour, 2010, 10(3): 564-567. doi: 10.1111/men.2010.10.issue-3
    [11]
    ROUSSET F. GENEPOP'007: a complete re-implementation of the GENEPOP software for Windows and Linux[J]. Mol Ecol Resour, 2008, 8(1): 103-106. doi: 10.1111/j.1471-8286.2007.01931.x
    [12]
    RICE W E. Analyzing tables of statistical tests[J]. Evolution, 1989, 43(1): 223-225. doi: 10.1111/evo.1989.43.issue-1
    [13]
    李小芳, 程晓凤, 王生, 等. 鲢微卫星标记的荧光多重PCR体系建立及其应用[J]. 农业生物技术学报, 2012, 20(7): 822-830. doi: 10.3969/j.issn.1674-7968.2012.07.013
    [14]
    苗贵东. 半滑舌鳎微卫星标记开发、应用及遗传连锁图谱的构建[D]. 青岛: 中国海洋大学, 2010: 51-62.
    [15]
    李佳凯, 王志勇, 韦信键, 等. 大黄鱼微卫星多重PCR体系的建立及其应用[J]. 水产学报, 2014, 38(4): 470-475.
    [16]
    FU J J, SHEN Y B, XU X Y, et al. Multiplex microsatellite PCR sets for parentage assignment of grass carp (Ctenopharyngodon idella)[J]. Aquacult Int, 2013, 21(6): 1195-1207. doi: 10.1007/s10499-013-9623-z
    [17]
    LIU T, LI Q, SONG J L, et al. Development of genomic microsatellite multiplex PCR using dye-labeled universal primer and its validation in pedigree analysis of Pacific oyster (Crassostrea gigas)[J]. J Ocean Univ China, 2017, 16(1): 151-160. doi: 10.1007/s11802-017-3121-2
    [18]
    WANG Y K, ZHAO J, LI W, et al. Development of a multiplex microsatellite PCR assay based on microsatellite markers for the mud carp, Cirrhinus molitorella[J]. J World Aquacult Soc, 2016, 47(2): 277-286. doi: 10.1111/jwas.2016.47.issue-2
    [19]
    YOON M Y, MOE K T, KIM D Y, et al. Genetic diversity and population structure analysis of strawberry (Fragaria × ananassa Duch.) using SSR markers[J]. Electron J Biotechn, 2012, 15(2): 1-16.
    [20]
    BANERJEE S, DAS M, MIR R R, et al. Assessment of genetic diversity and population structure in a selected germplasm collection of 292 jute genotypes by microsatellite (SSR) markers[J]. Mol Plant Breed, 2012, 3(2): 11-25.
    [21]
    SOUZA I, SOUZA V, LIMA P. Molecular characterization of Platonia insignis Mart. ("Bacurizeiro") using inter simple sequence repeat (ISSR) markers[J]. Mol Biol Rep, 2013, 40(5): 3835-3845. doi: 10.1007/s11033-012-2462-6
    [22]
    徐艳. 橄榄蛏蚌微卫星标记开发及群体遗传多样性分析[D]. 武汉: 华中农业大学, 2014: 19-37.
    [23]
    NICAL K M, YOUNG W P, DANZMANN R G. A consolidated linkage map for rainbow trout[J]. Anim Genet, 2003, 34(2): 102-115. doi: 10.1046/j.1365-2052.2003.00957.x
    [24]
    梁利群, 常玉梅, 董崇智, 等. 微卫星DNA标记对乌苏里江哲罗鱼遗传多样性的分析[J]. 水产学报, 2004, 28(3): 241-244.
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