AFLP analysis on original population of mud carp Cirrhinus molitorella
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Graphical Abstract
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Abstract
Amplified fragment length polymorphism (AFLP) technique was applied to assess the genetic variations of the original pop-ulation collected from Original Species Field of Cirrhinus molitorella in Guangdong Province. The studied samples contained sub-stock h and sub-stock q, 29 individuals respectively. Amplifications with 6 pairs of primers gave 173 reproducible and stable fragments, of which 72 were polymorphic. There was one band whose frenquency was much higher in sub-stock h than that was in sub-stock q (72.4%>20.6%). We named it as E2M4-1. Based on AFLP data, we found that the genetic diversity of sub-stock h was higher than sub-stock q (0.1367>0.0998). This study is a preliminary attempt for special molecular marker selecting of the two wild sub-stocks.
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