AFLP analysis on original population of mud carp Cirrhinus molitorella
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Abstract
Amplified fragment length polymorphism (AFLP) technique was applied to assess the genetic variations of the original population collected from Original Species Field of Cirrhinus molitorella in Guangdong Province. The studied samples contained sub-stock h and sub-stock q, 29 individuals respectively. Amplifications with 6 pairs of primers gave 173 reproducible and stable fragments, of which 72 were polymorphic. There was one band whose frenquency was much higher in sub-stock h than that was in sub-stock q (72.4%20.6%). We named it as E2M4-1. Based on AFLP data, we found that the genetic diversity of sub-stock h was higher than sub-stock q (0.13670.0998). This study is a preliminary attempt for special molecular marker selecting of the two wild sub-stocks.
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