DOU Yong, QIAO Xiuting, CHEN Limei, ZHOU Wenli. Study on oxidative stress and damage of Cyclina sinensis exposed to naphthalene[J]. South China Fisheries Science, 2014, 10(4): 39-44. DOI: 10.3969/j.issn.2095-0780.2014.04.007
Citation: DOU Yong, QIAO Xiuting, CHEN Limei, ZHOU Wenli. Study on oxidative stress and damage of Cyclina sinensis exposed to naphthalene[J]. South China Fisheries Science, 2014, 10(4): 39-44. DOI: 10.3969/j.issn.2095-0780.2014.04.007

Study on oxidative stress and damage of Cyclina sinensis exposed to naphthalene

More Information
  • Received Date: March 12, 2014
  • Revised Date: April 15, 2014
  • The SOD, CAT, AKP activities and MDA content were determined after Cyclina sinensis were exposed to different concentrations (2 mg·L-1, 8 mg·L-1 and 32 mg·L-1) of naphthalene under experimental ecotoxicology conditions. The results indicate that C.sinensis had high mortality rate under 32 mg·L-1 of naphthalene. Under non-lethal conditions and with increase of naphthalene exposure time, SOD activity showed a continuous increasing trend; CAT activity was promoted in low-concentration group and inhibited in high-concentration group; AKP activity was initially increasing then decreasing; MDA content fluctuated following the trend of low→high→low. According to Pearson correlation analysis, SOD and CAT showed synergistic effect (R=0.439, P < 0.01), while SOD and AKP showed antagonism effect (R=-0.571, P < 0.01). CAT and AKP might protect cellular membrane structure better than SOD (R=-0.490, P < 0.01).

  • [1]
    孙闰霞, 林钦, 柯常亮, 等. 海洋生物体多环芳烃污染残留及其健康风险评价研究[J]. 南方水产科学, 2012, 8(3): 71-78. doi: 10.3969/j.issn.2095-0780.2012.03.011
    [2]
    孟范平, 程凤莲, 王建春, 等. 基于氧化逆境标志物的北部湾潮间带沉积物有机污染程度综合评价[J]. 海洋学报, 2013, 35(3): 155-165. doi: 10.3969/j.issn.0253-4193.2013.03.018
    [3]
    高萍, 夏斌, 崔毅, 等. 菲律宾蛤仔对石油烃的富集与释放特征[J]. 海洋环境科学, 2013, 32(1): 63-66. https://d.wanfangdata.com.cn/periodical/hyhjkx201301014
    [4]
    贾晓平, 林钦, 蔡文贵, 等. 海洋动物体石油烃污染评价标准参考值的探讨[J]. 湛江海洋大学学报, 1999, 19(3): 33-37. https://www.cqvip.com/qk/93246a/1999003/4784760.html
    [5]
    马继臻, 袁骐, 蒋玫, 等. 东海沿岸不同区域贝类体内石油烃含量的分布特征及其评价[J]. 海洋通报, 2008, 27(2): 73-78. doi: 10.3969/j.issn.1001-6392.2008.02.011
    [6]
    苗晶晶, 潘鲁青, 王静. 苯并[a]芘对栉孔扇贝(Chlamys farreri)组织显微和超微结构的影响[J]. 环境科学学报, 2007, 27(9): 1497-1503. doi: 10.3321/j.issn:0253-2468.2007.09.014
    [7]
    王清, 杨红生, 王晓宇. 镉和苯并芘胁迫对文蛤血细胞功能的影响[J]. 海洋科学, 2010, 34(9): 82-86. https://d.wanfangdata.com.cn/periodical/hykx201009015
    [8]
    丁鉴锋, 闫喜武, 赵力强, 等. 海洋污染物对菲律宾蛤仔的免疫毒性[J]. 生态学报, 2013, 33(17): 5419-5425. doi: 10.5846/stxb201206250890
    [9]
    UMBUZEIRO G, ALEXANDRE F, MARTINS M, et al. Mutagenicity and DNA adduct formation of PAH, nitro-PAH, and oxy-PAH fractions of atmospheric particulate matter from Sao Paulo, Brazil[J]. Mutat Res, 2008, 652 (1): 72-80. doi: 10.1016/j.mrgentox.2007.12.007
    [10]
    PISONI M, COGOTZI L, FRIGERI A, et al. DNA adducts, benzo(a)pyrene monooxygenase activity, and lysosomal membrane stability in Mytilus galloprovincialis from different areas in Taranto coastal waters (Italy)[J]. Environ Res, 2004, 96 (2): 163-175. doi: 10.1016/j.envres.2004.02.011
    [11]
    AAS E, BAUSSANT T, BALK L, et al. PAH metabolites in bile, cytochrome P4501A and DNA adducts as environmental risk parameters for chronic oil exposure: a laboratory experiment with Atlantic cod[J]. Aquat Toxicol, 2000, 51 (2): 241-258. doi: 10.1016/S0166-445X(00)00108-9
    [12]
    ΦSTBYA L, GABRIELSENB G, KRΦKJE A. Cytochrome P4501A induction and DNA adduct formation in glaucous gulls(Larus hyperboreus), fed with environmentally contaminated gull eggs[J]. Ecotox Environ Safe, 2005, 62 (3): 363-375. doi: 10.1016/j.ecoenv.2004.12.023
    [13]
    TAIOLI E, RADIM S, BLANKA B, et al. Biomarkers of exposure to carcinogenic PAHs and their relationship with environmental factors[J]. Mutat Res, 2007, 620 (1/2): 16-21. https://www.sciencedirect.com/science/article/pii/S0027510707000991
    [14]
    王丽平, 郑丙辉, 孟伟. 环境污染物对水生生物产生氧化压力的分子生物标志物[J]. 生态学报, 2007, 27(1): 380-388. doi: 10.3321/j.issn:1000-0933.2007.01.044
    [15]
    杨涛, 陈海刚, 蔡文贵, 等. 菲和苯并(b)荧蒽曝露对翡翠贻贝外套膜的氧化胁迫及损伤[J]. 南方水产科学, 2011, 7(4): 24-29. doi: 10.3969/j.issn.2095-0780.2011.04.004
    [16]
    李学峰, 周启星, 罗义. 甲苯、乙苯和二甲苯对中华新米虾的毒性效应[J]. 中国环境科学, 2013, 33(3): 530-537. doi: 10.3969/j.issn.1000-6923.2013.03.022
    [17]
    隋亚栋. 阴离子表面活性剂SDS和多环芳烃蒽对菲律宾蛤仔(Ruditapes pilippinarum)的毒性效应研究[D]. 青岛: 中国海洋大学, 2011. https://www.doc88.com/p-9542111619514.html
    [18]
    黄周英, 陈奕欣, 赵扬, 等. 三丁基锡对文蛤鳃酸性磷酸酶、碱性磷酸酶和Na+, K+ -ATP酶活性的影响[J]. 海洋环境科学, 2005, 24(3): 56-59. doi: 10.3969/j.issn.1007-6336.2005.03.015
    [19]
    余群, 王重刚, 陈荣, 等. 硒对华贵栉孔扇贝碱性磷酸酶的酶动力学及理化性质研究[J]. 海洋科学, 2008, 32(10): 56-61. https://d.wanfangdata.com.cn/periodical/hykx200810011
    [20]
    陈荣, 潘文扬. 厦门海域僧帽牡蛎生化指标与有机污染物的相关性[J]. 中国环境科学, 2006, 26(4): 418-421. doi: 10.3321/j.issn:1000-6923.2006.04.008
    [21]
    王丽平, 郑丙辉, 孟伟. 多环芳烃对卤虫无节幼体的光诱导毒性[J]. 环境科学学报, 2008, 28(4): 748-754. doi: 10.3321/j.issn:0253-2468.2008.04.022
    [22]
    王悠, 姜爽, 赵晓玮, 等. 2种有机污染物对鲈鱼生化标志物系统的影响及作用评价[J]. 环境科学, 2010, 31(3): 801-807. https://d.wanfangdata.com.cn/periodical/hjkx201003040
    [23]
    BINELLI A, PROVINI A. POPs in edible clams from different Italian and European markets and possible human health risk[J]. Mar Pollut Bull, 2003, 46(7) : 879-886. doi: 10.1016/S0025-326X(03)00043-2
    [24]
    AKCHA F, HUBERT F, LESZKOWICZ A. Potential value of the comet assay and DNA adduct measurement in dab (Limanda limanda) for assessment of in situ exposure to genotoxic compounds[J]. Mutat Res, 2003, 534 (1/2): 21-32. doi: 10.1016/S1383-5718(02)00244-9
    [25]
    BINKOVA B, IRENA C, ZDENA L, et al. PAH-DNA adducts in environmentally exposed population in relation to metabolic and DNA repair gene polymorphisms[J]. Mutat Res, 2007, 620 (1/2): 49-61. doi: 10.1016/j.mrfmmm.2007.02.022
    [26]
    MCCLAIN J S, ORIS J T, BURTON G A, et al. Laboratory and field validation of multiple molecular biomarkers of contaminant exposure in rainbow trout(Oncorhynchus mykiss)[J]. Environ Toxicol Chem, 2003, 22 (2): 361-370. doi: 10.1002/etc.5620220217
    [27]
    REGOLI F, WINSTON G W, GORBI S, et al. Integrating enzymatic response to organicchemical exposure with total oxyradical absorbing capacity and DNA damage in the European eel Anguilla Anguilla[J]. Environ Toxicol Chem, 2003, 22 (9): 2120-2129. doi: 10.1897/02-378
    [28]
    靳永轩, 刘晨临, 丛柏林, 等. 栉孔扇贝(Chlamys farreri)细胞色素CYP4基因表达对石油烃污染的响应特征[J]. 海洋环境科学, 2013, 32(2): 257-261. https://d.wanfangdata.com.cn/periodical/hyhjkx201302021
    [29]
    畅悦, 冯立芳, 缪炜. 有机污染物二氯二苯三氯乙烷、三丁基锡和2, 3, 7, 8-四氯二苯并二噁英暴露下的四膜虫毒理基因组学研究[J]. 中国科学: 生命科学, 2011, 41(6): 502-511. doi: 10.1360/zc2011-41-6-502
  • Related Articles

    [1]CHEN Li, XU Jiaxin, LI Liujia, ZHAO Chengfa, LONG Xiaowen. Effects of dietary fishmeal replacement by Periplaneta americana meal on biochemical indexes, disease resistance and metabolomics of juvenile Oncorhynchus mykiss[J]. South China Fisheries Science, 2023, 19(4): 86-97. DOI: 10.12131/20220208
    [2]WANG Wenwen, ZHOU Shengjie, HU Jing, FU Zhengyi, XIAO Bo, DU Hengyi, YANG Rui, YU Gang, MENG Xiangjun, MA Zhenhua. Effects of different light intensity on plasma biochemical indexes of Euthynnus affinis on sunny and cloudy days[J]. South China Fisheries Science, 2023, 19(2): 170-176. DOI: 10.12131/20220224
    [3]ZENG Xiangbing, DONG Hongbiao, WEI Zhengkun, DUAN Yafei, CHEN Jian, ZHANG Hui, SUN Caiyun, XU Xiaodong, ZHANG Jiasong. Effects of polysaccharide from Endothelium corneum gigeriae galli on growth, digestive, intestinal antioxidant capacity and serum biochemical indices of Lates calcarifer[J]. South China Fisheries Science, 2021, 17(4): 49-57. DOI: 10.12131/20210028
    [4]WANG Haifeng, CHENG Yongxu, LI Jinghao, XI Yewen, LI Jiayao. Effects of desiccation and resubmersion on oxidative stress response of crayfish (Procambarus clarkii)[J]. South China Fisheries Science, 2019, 15(5): 69-76. DOI: 10.12131/20190059
    [5]WU Fan, JIANG Ming, WEN Hua, LIU Wei, TIAN Juan, YU Lijuan, LU Xing. Effects of dietary carbohydrate to lipid ratio on growth performance, body composition and serum biochemical indices of adult GIFT Oreochromis niloticus[J]. South China Fisheries Science, 2019, 15(4): 53-60. DOI: 10.12131/20190047
    [6]TAN Lianjie, LIN Heizhao, HUANG Zhong, XUN Pengwei, HUANG Qianqian, ZHOU Chuanpeng, HUANG Xiaolin, YU Wei. Effect of dietary angelica polysaccharide (AP) on growth performance, antioxidant capacity, serum immune and serum biochemical indices of juvenile golden pompano (Trachinotus ovatus)[J]. South China Fisheries Science, 2018, 14(4): 72-79. DOI: 10.3969/j.issn.2095-0780.2018.04.009
    [7]OU Youjun, CHEN Shixi, WANG Pengfei, LI Jia'er, WEN Jiufu, WANG Wen, XIE Mujiao. Study on oxidative stress response and physiological metabolism related indices of Trachinotus ovatus under hyp-oxia stress[J]. South China Fisheries Science, 2017, 13(3): 120-124. DOI: 10.3969/j.issn.2095-0780.2017.03.016
    [8]LIU Xujia, HUANG Guoqiang, PENG Yinhui. Effect of different dissolved oxygen levels on growth, energy metabolism and oxidative stress of Mugil cephalus[J]. South China Fisheries Science, 2015, 11(4): 88-94. DOI: 10.3969/j.issn.2095-0780.2015.04.013
    [9]WANG Guoxia, LIU Qunfang, HUANG Wenqing, LIN Jianan, HUANG Yanhua, XU Liming. Effects of complete enzyme preparation on growth performance, serum biochemical and immune indices of juvenile yellow catfish (Peltobagrus fulvidraco)[J]. South China Fisheries Science, 2013, 9(6): 84-89. DOI: 10.3969/j.issn.2095-0780.2013.06.014
    [10]YUAN Fenghua, LIN Heizhe, LI Zhuojia, LU Xin, YANG Qibin. Effects of dietary Bacillus licheniformis on blood physiological-biochemical indices in cultured Lates calarifer[J]. South China Fisheries Science, 2009, 5(2): 45-50. DOI: 10.3969/j.issn.1673-2227.2009.02.008
  • Cited by

    Periodical cited type(9)

    1. 杜欣欣,刘福利,袁艳敏,梁洲瑞,张朋艳,刘升平. 极北海带幼苗对氮、磷和铁营养元素需求的研究. 水产科学. 2021(01): 81-88 .
    2. 袁艳敏,刘福利,梁洲瑞,王潇. 氮营养盐对极北海带幼苗生长和生理生化特性的影响. 水产科学. 2021(02): 203-209 .
    3. 吴翔宇,黄良夫,杨守国,曾俊,邢诒炫. 芋根江蓠对对虾养殖水体氮磷的去除效果. 现代农业科技. 2021(08): 178-179 .
    4. 曾俊,童立豪,吴翔宇,黄敏,唐贤明. 不同营养盐条件对琼枝生长的影响. 渔业科学进展. 2021(04): 168-176 .
    5. 叶鹏浩,韩婷婷,付贵权,谷阳光,黄洪辉. 半叶马尾藻对重金属镉胁迫的生理响应. 南方水产科学. 2019(05): 35-40 . 本站查看
    6. 韩婷婷,齐占会,黄洪辉,梁庆洋. 3种氮源加富对半叶马尾藻幼苗生长和生化组成的影响. 渔业科学进展. 2018(04): 159-166 .
    7. 张文文,郭永坚,李俊伟,朱长波,陈素文,颉晓勇. 营养盐对海萝生长和藻体生化成分的影响. 南方水产科学. 2016(02): 30-35 . 本站查看
    8. 王云祥,李正,秦传新,陈丕茂,袁华荣,周文礼. 不同季节江蓠脱落物对大型海藻场上覆水的影响. 南方水产科学. 2016(02): 13-20 . 本站查看
    9. 戴晓玲,李文红,章增林,庞洋洋,黄柳琪. 芋根江蓠和异枝江蓠早期腐解过程及其氮磷释放规律的研究. 上海海洋大学学报. 2016(04): 582-590 .

    Other cited types(8)

Catalog

    Article views (3207) PDF downloads (1542) Cited by(17)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return