Comparative transcriptome analysis in livers of female and male marine medaka (<i>Oryzias melastigma</i>)

ZHANG Linbao; TIAN Fei; CHEN Haigang; ZHANG Zhe; YE Guoling; LI Yitong; TANG Haiwei

doi:10.12131/20220250

Volume 19 Issue 3

Jun. 2023

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South China Fisheries Science > 2023 > 19(3): 88-97. > DOI: 10.12131/20220250

ZHANG Linbao, TIAN Fei, CHEN Haigang, ZHANG Zhe, YE Guoling, LI Yitong, TANG Haiwei. Comparative transcriptome analysis in livers of female and male marine medaka (Oryzias melastigma)[J]. South China Fisheries Science, 2023, 19(3): 88-97. DOI: 10.12131/20220250

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Comparative transcriptome analysis in livers of female and male marine medaka (Oryzias melastigma)

South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of South China Sea Fishery Resource and Environment, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China

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Received Date: September 16, 2022
Revised Date: November 27, 2022
Accepted Date: December 07, 2022
Available Online: December 18, 2022

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Abstract

Abstract

As a gonochoristic model animal, marine medaka (Oryzias melastigma) is good for studying the sex-specific responses of organisms to xenobiotic pollutants. We used comparative transcriptomics technology to systematically investigate the differentially expressed genes (DEGs) between the liver tissues of female and male medaka. We identified 683 significantly up-regulated DEGs in the females, and 668 DEGs in the males. The high expressed DEGs in the females were involved in the reproductive and sex hormone synthesis pathways, such as vitellogenin and estrogen receptor. The top twenty DEGs in the males were involved in energy metabolism, cytoskeleton and muscle contraction, such as pyruvate kinase, creatine kinase, myosin and troponin. Except for the DNA mismatch repair protein, all the 17 DEGs had similar magnitude and expression trends by both qRT-PCR and RNA-seq analyses, which confirms the reliability of the RNA-seq data. The results demonstrate that the gene expression patterns are different in the livers of female and male medaka, and the DEGs provide a theoretical basis for promoting the molecular mechanism of sex-specific responses of medaka to xenobiotic pollutants.
- Oryzias melastigma,
- Liver,
- Sex,
- Transcriptome,
- Differential expression genes

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Comparative transcriptome analysis in livers of female and male marine medaka (Oryzias melastigma)

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