Response to UV-B radiation and physiological mechanism of marine microalga <i>Asterarcys</i> sp.

LYU Jinting; PEI Haiwei; WEI Huaning; WU Hualian; LI Tao; WU Houbo; XIANG Wenzhou

doi:10.12131/20220140

In order to investigate the response of microalgae to UV-B radiation and their physiological mechanism, and to improve their potential value, we selected Asterarcys sp. SCSIO-44020 as material, and exposed them to UV-B radiation under different radiation conditions (0, 10, 30, 50 and 70 min each time, corresponding to radiation doses of 0, 150, 460, 770 and 1 050 mJ·cm⁻²) every 48 h until the end of the culture. The results show that, after a 20-day cultivation, UV-B radiation of 10–50 min treatment group had no significant effect on the growth of algae compared with the control group (P>0.05), but the 70 min treatment group inhibited the growth significantly (P<0.05). With the increase of UV-B radiation time, the protein content increased gradually, but the total lipid content decreased, with no siginificant differences between different groups and the control group (P>0.05). The total carotenoid and mycosporine-like amino acids (MAAs) contents increased with the increase of UV-B treatment time, and reached the maximum values in 70 min treatment group, 1.75 times that of the control group. However, compared with the control group, the total carbohydrates and MAAs yields in 50 min treatment group increased 12.40% and 57.61%, respectively, reaching the maximum values. In conclusion, Asterarcys sp. has a strong ability to resist UV-B radiation, and the increase of MAAs is the key to its adapting to UV radiation. Moreover, intermittent treatment of 50 min UV-B radiation is an effective approach to regulate the production of MAAs and other products in microalga.

[1]	魏静, 林莉, 潘雄, 等. 不同环境胁迫因子对藻类分子生物学特性的影响研究进展[J]. 长江科学院院报, 2020, 37(4): 14-24. doi: 10.11988/ckyyb.20190062
[2]	RASTOGI R P, MADAMWAR D, NAKAMOTO H, et al. Resilience and self-regulation processes of microalgae under UV radiation stress[J]. J Photochem Photobiol C, 2020, 43: 100322. doi: 10.1016/j.jphotochemrev.2019.100322
[3]	HÄDER D P, WILLIAMSON C E, WÄNGBERG S Å, et al. Effects of UV radiation on aquatic ecosystems and interactions with other environmental factors[J]. Photochem Photobiol Sci, 2015, 14(1): 108-126.
[4]	EL-SHEEKH M M, ALWALEED E A, IBRAHIM A, et al. Detrimental effect of UV-B radiation on growth, photosynthetic pigments, metabolites and ultrastructure of some cyanobacteria and freshwater chlorophyta[J]. Int J Radiat Biol, 2021, 97(2): 265-275. doi: 10.1080/09553002.2021.1851060
[5]	缪锦来, 阚光锋, 李光友, 等. UV-B辐照培养下南极冰藻的形态和超微结构及主要生化组成的变化[J]. 中国海洋药物, 2003(6): 1-5. doi: 10.3969/j.issn.1002-3461.2003.06.001
[6]	KURINJIMALAR C, KAVITHA G, THEVANATHAN R, et al. Impact of ultraviolet-B radiation on growth and biochemical composition of Botryococcus braunii Kutz.[J]. Curr Sci, 2019, 116(1): 89. doi: 10.18520/cs/v116/i1/89-95
[7]	FUENTES-TRISTAN S, PARRA-SALDIVAR R, IQBAL H M N, et al. Bioinspired biomolecules: mycosporine-like amino acids and scytonemin from Lyngbya sp. with UV-protection potentialities[J]. J Photochem Photobiol B, 2019, 201: 111684. doi: 10.1016/j.jphotobiol.2019.111684
[8]	SABER H, EL-SHEEKH M M, IBRAHIM A, et al. Effect of UV-B radiation on amino acids profile, antioxidant enzymes and lipid peroxidation of some cyanobacteria and green algae[J]. Int J Radiat Biol, 2020, 96(9): 1192-1206. doi: 10.1080/09553002.2020.1793025
[9]	VARSHNEY P, BEARDALL J, BHATTACHARYA S, et al. Isolation and biochemical characterisation of two thermophilic green algal species-Asterarcys quadricellulare and Chlorella sorokiniana, which are tolerant to high levels of carbon dioxide and nitric oxide[J]. Algal Res, 2018, 30: 28-37. doi: 10.1016/j.algal.2017.12.006
[10]	SINGH D P, KHATTAR J S, RAJPUT A, et al. High production of carotenoids by the green microalga Asterarcys quadricellulare PUMCC 5.1.1 under optimized culture conditions[J]. PLoS One, 2019, 14(9): e0221930. doi: 10.1371/journal.pone.0221930
[11]	LI T, YANG F F, XU J, et al. Evaluating differences in growth, photosynthetic efficiency, and transcriptome of Asterarcys sp. SCS-1881 under autotrophic, mixotrophic, and heterotrophic culturing conditions[J]. Algal Res, 2020, 45: 101753. doi: 10.1016/j.algal.2019.101753
[12]	HONG J W, KIM S, CHANG J W, et al. Isolation and description of a Korean microalga, Asterarcys quadricellulare KNUA020, and analysis of its biotechnological potential[J]. Algae, 2012, 27(3): 197-203. doi: 10.4490/algae.2012.27.3.197
[13]	卫华宁, 王灵, 李涛, 等. 不同氮源及氮浓度对海水驯化藻株Asterarcys sp. 生长及生化组成的影响[J]. 生物技术通报, 2021, 37(10): 34-44.
[14]	KHOZIN-GOLDBERG I, SHRESTHA P, COHEN Z. Mobilization of arachidonyl moieties from triacylglycerols into chloroplastic lipids following recovery from nitrogen starvation of the microalga Parietochloris incisa[J]. Biochim Biophys Acta Mol Cell Biol Lipids, 2005, 1738(1/2/3): 63-71.
[15]	LI T, XU J, GAO B Y, et al. Morphology, growth, biochemical composition and photosynthetic performance of Chlorella vulgaris (Trebouxiophyceae) under low and high nitrogen supplies[J]. Algal Res, 2016, 16: 481-491. doi: 10.1016/j.algal.2016.04.008
[16]	LI T, WAN L L, LI A F, et al. Responses in growth, lipid accumulation, and fatty acid composition of four oleaginous microalgae to different nitrogen sources and concentrations[J]. Chin J Oceanol Limnol, 2013, 31(6): 1306-1314. doi: 10.1007/s00343-013-2316-7
[17]	李嘉颖, 李涛, 谭丽, 等. 盐度对一株淡水栅藻Scenedesmus sp. 生长及生化组成的影响[J]. 生物技术通报, 2017, 33(7): 155-161.
[18]	吴燕燕, 张婉, 李来好, 等. 海萝藻中类菌胞素氨基酸的种类分析及抗氧化性能[J]. 中国食品学报, 2018, 18(5): 264-272. doi: 10.16429/j.1009-7848.2018.05.032
[19]	CHANDRA R, PONS-FAUDOA F P, SALDÍVAR R P, et al. Effect of ultra-violet exposure on production of mycosporine-like amino acids and lipids by Lyngbya purpurem[J]. Biomass Bioenerg, 2020, 134: 105475. doi: 10.1016/j.biombioe.2020.105475
[20]	SINGH G, BABELE P K, SINHA R P, et al. Enzymatic and non-enzymatic defense mechanisms against ultraviolet-B radiation in two Anabaena species[J]. Process Biochem, 2013, 48(5/6): 796-802. doi: 10.1016/j.procbio.2013.04.022
[21]	SINGH A, TYAGI M B, KUMAR A. Cyanobacteria growing on tree barks possess high amount of sunscreen compound mycosporine-like amino acids (MAAs)[J]. Plant Physiol Biochem, 2017, 119: 110-120. doi: 10.1016/j.plaphy.2017.08.020
[22]	SHEN S G, GUO R J, YAN R R, et al. Comparative proteomic analysis of Nostoc flagelliforme reveals the difference in adaptive mechanism in response to different ultraviolet-B radiation treatments[J]. Mol Biol Rep, 2018, 45(6): 1995-2006. doi: 10.1007/s11033-018-4355-9
[23]	XUE L G, ZHANG Y, ZHANG T G, et al. Effects of enhanced ultraviolet-B radiation on algae and cyanobacteria[J]. Crit Rev Microbiol, 2005, 31(2): 79-89. doi: 10.1080/10408410590921727
[24]	LAURION I, ROY S. Growth and photoprotection in three dinoflagellates (including two strains of Alexandrium tamarense) and one diatom exposed to four weeks of natural and enhanced UVB radiation[J]. J Phycol, 2009, 45(1): 16-33. doi: 10.1111/j.1529-8817.2008.00618.x
[25]	孙小琴, 孙昕, 李鹏飞, 等. 紫外辐射对小球藻光合性能及油脂积累的影响[J]. 中国油脂, 2019, 44(12): 114-119.
[26]	CHEN H, HUANG K X, LIU S S, et al. Effects of ultraviolet (UV) radiation on outdoor-and indoor-cultured Prorocentrum lima, a toxic benthic dinoflagellate[J]. J Ocean Univ, 2021, 20(3): 619-628. doi: 10.1007/s11802-021-4560-3
[27]	屠燕萍, 俞泓伶, 谢志浩. 三角褐指藻和小角毛藻对UV-B辐射增强的生理生化响应[J]. 生态科学, 2013, 32(4): 474-479.
[28]	FU S M, XUE S, CHEN J, et al. Effects of different short-term UV-B radiation intensities on metabolic characteristics of Porphyra haitanensis[J]. Int J Mol Sci, 2021, 22(4): 2180. doi: 10.3390/ijms22042180
[29]	NOAMAN N H, AKL F, ABDEL-KAREEM M S M, et al. Effect of Ultraviolet-B irradiation on fatty acids, amino acids, protein contents, enzyme activities and ultrastructure of some algae[J]. Eur J Phycol, 2013, 14(1): 67-101.
[30]	王静, 郭照冰, 王瑾瑾, 等. UV-B对紫球藻生长抑制及生理特性的影响[J]. 水生态学杂志, 2018, 39(6): 114-120. doi: 10.15928/j.1674-3075.2018.06.017
[31]	GONZALEZ-SILVERA D, PÉREZ S, KORBEE N, et al. Effects of global change factors on fatty acids and mycosporine-like amino acid production in Chroothece richteriana (Rhodophyta)[J]. J Phycol, 2017, 53(5): 999-1009. doi: 10.1111/jpy.12560
[32]	NAVARRO N P, MANSILLA A, FIGUEROA F L, et al. Short-term effects of solar UV radiation and NO₃ ⁻ supply on the accumulation of mycosporine-like amino acids in Pyropia columbina (Bangiales, Rhodophyta) under spring ozone depletion in the sub-Antarctic region, Chile[J]. Bot Marina, 2014, 57(1): 9-20. doi: 10.1515/bot-2013-0090
[33]	RASTOGI R P, INCHAROENSAKDI A. UV radiation-induced accumulation of photoprotective compounds in the green alga Tetraspora sp. CU2551[J]. Plant Physiol Biochem, 2013, 70: 7-13. doi: 10.1016/j.plaphy.2013.04.021
[34]	DIEHL N, MICHALIK D, ZUCCARELLO G C, et al. Stress metabolite pattern in the eulittoral red alga Pyropia plicata (Bangiales) in New Zealand-mycosporine-like amino acids and heterosides[J]. J Exp Mar Biol Ecol, 2019, 510: 23-30. doi: 10.1016/j.jembe.2018.10.002
[35]	张英莲. UV-B辐射对华南沿海常见赤潮藻类生长和类菌孢素氨基酸 (MAAs) 含量的影响[D]. 广州: 华南师范大学, 2007: 26-33.
[36]	王婉如, 张昺林, 张楠, 等. 蓝藻对UV-B增强的响应及其紫外屏蔽物质的研究[J]. 天然产物研究与开发, 2012, 24(9): 1303-1311, 1329. doi: 10.3969/j.issn.1001-6880.2012.09.034
[37]	NASSOUR R, AYASH A. Effects of ultraviolet-B radiation in plant physiology[J]. Agric (Pol'nohospodárstvo), 2021, 67(1): 1-15.
[38]	van den ENDE W, VALLURU R. Sucrose, sucrosyl oligosaccharides, and oxidative stress: scavenging and salvaging?[J]. J Exp Bot, 2009, 60(1): 9-18.
[39]	DEMIR E, KOCAOĞLU S, KAYA B. Protective effects of chlorophyll against the genotoxicity of UVB in Drosophila smart assay[J]. Fresenius Environ Bull, 2008, 17(12): 2180-2186.
[40]	SHEN S G, JIA S R, YAN R R, et al. The physiological responses of terrestrial cyanobacterium Nostoc flagelliforme to different intensities of ultraviolet-B radiation[J]. RSC Adv, 2018, 8(38): 21065-21074. doi: 10.1039/C8RA04024A
[41]	涂勃. 阳光UV辐射对铜绿微囊藻生理生化特性影响的研究[D]. 武汉: 湖北工业大学, 2018: 5-6.

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Response to UV-B radiation and physiological mechanism of marine microalga Asterarcys sp.

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Response to UV-B radiation and physiological mechanism of marine microalga Asterarcys sp.

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