Volume 15 Issue 3
Jun.  2019
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KONG Xiaolan, LI Min, CHEN Zuozhi, GONG Yuyan, ZHANG Jun, ZHANG Peng. Development and evaluation of di-/tri-nucleotide-repeated microsatellites by RAD-seq in Decapterus macrosoma[J]. South China Fisheries Science, 2019, 15(3): 97-103. DOI: 10.12131/20180256
Citation: KONG Xiaolan, LI Min, CHEN Zuozhi, GONG Yuyan, ZHANG Jun, ZHANG Peng. Development and evaluation of di-/tri-nucleotide-repeated microsatellites by RAD-seq in Decapterus macrosoma[J]. South China Fisheries Science, 2019, 15(3): 97-103. DOI: 10.12131/20180256
shu

Development and evaluation of di-/tri-nucleotide-repeated microsatellites by RAD-seq in Decapterus macrosoma

  • Key Laboratory of Open-Sea Fishery Development , Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

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  • Received Date: November 21, 2018
  • Revised Date: January 23, 2019
  • Accepted Date: February 18, 2019
  • Available Online: February 19, 2019
    Graphical Abstract
    • Abstract

  • A total of 58 180 microsatellites were developed by RAD-Seq high-throughput sequencing technology in Decapterus macrosoma. One hundred and twelve microsatellite loci were randomly selected from di-/tri-nucleotide repeat microsatellite loci. Finally, twenty-seven highly polymorphic markers were developed. The population genetic analysis finds 18 dinucleotide-repeated microsatellite loci (9−14 repeated) and 9 trinucleotide-repeated microsatellite loci (6−10 repeated). The number of alleles (Na) ranged from 5 to 17 (mean 10.6). The observed and expected heterozygosities ranged from 0.342 9 to 0.857 1 (mean 0.631 7) and 0.538 3 to 0.911 8 (mean 0.796 8), respectively. The polymorphism information content (PIC) ranged from 0.497 to 0.886 (mean 0.780 9), and the PIC at all loci except for one was geater than 0.500, indicating that the microsatellites were highly polymorphic. The Hardy-Weinberg equilibrium (HWE) test shows that 19 microsatellite markers conformed to HWE. There is no linkage imbalance among the 27 loci. The 27 microsatellites developed by this study will be useful for further population genetic analysis.

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