Volume 11 Issue 4
Aug.  2015
Turn off MathJax
Article Contents
Abstract
References
Related Articles
YANG Bing, LIN Lin, LI Chunhou, XU Shannan, LIU Yong, XIAO Yayuan, CHEN Zuozhi. Development and evaluation of microsatellite markers in Parargyrops edita[J]. South China Fisheries Science, 2015, 11(4): 116-120. DOI: 10.3969/j.issn.2095-0780.2015.04.017
Citation: YANG Bing, LIN Lin, LI Chunhou, XU Shannan, LIU Yong, XIAO Yayuan, CHEN Zuozhi. Development and evaluation of microsatellite markers in Parargyrops edita[J]. South China Fisheries Science, 2015, 11(4): 116-120. DOI: 10.3969/j.issn.2095-0780.2015.04.017
shu

Development and evaluation of microsatellite markers in Parargyrops edita

  • 1.

    Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

More Information
  • Received Date: March 24, 2015
  • Revised Date: April 19, 2015
    Graphical Abstract
    • Abstract

  • We isolated and characterized 15 polymorphic microsatellite markers in crimson seabream (Parargyrops edita) using high-throughput sequencing and polyacrylamide gel electrophoresis (PAGE), having obtained 0.12 Gbp spliced sequences of P.edita and searched 292 microsatellite sequences with suitable flanking regions from the spliced sequences. Then we designed 40 primer pairs in the microsatellite sequences for amplification by polymerase chain reaction (PCR), among which 15 primer pairs were clearly amplified and shown to be polymorphic. We found 2~16 alleles per locus (averagely 6.93). The observed and expected heterozygosities ranged from 0.268 to 0.979 and from 0.300 to 0.914, with an average of 0.568 and 0.640, respectively. No loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni correction, except for three loci. These markers will be useful for analyzing the population genetic diversity characteristics of P.edita.

    • FullText(HTML)
    • References (28)
  • [1]
    陈作志, 邱永松. 北部湾二长棘鲷的资源变动[J]. 南方水产, 2005, 1(3): 26-31. doi: 10.3969/j.issn.2095-0780.2005.03.004
    [2]
    赵传絪, 刘效顺, 曾炳光, 等. 中国海洋渔业资源[M]. 杭州: 浙江科学技术出版社, 1990: 55-56.
    [3]
    陈作志, 邱永松. 北部湾二长棘鲷生长和死亡参数估计[J]. 水产学报, 2003, 27(3): 251-257. doi: 10.3321/j.issn:1000-0615.2003.03.010
    [4]
    陈作志, 邱永松. 南海区海洋渔业资源现状和可持续利用对策[J]. 湖北农学院学报, 2002, 22(6): 507-510. https://xueshu.baidu.com/usercenter/paper/show?paperid=d158c197849ba1a8eb5f9f7adaba97cc&site=xueshu_se&hitarticle=1
    [5]
    陈作志, 邱永松. 北部湾二长棘鲷的生态分布[J]. 海洋水产研究, 2005, 26(3): 16-21. doi: 10.3969/j.issn.1000-7075.2005.03.003
    [6]
    王跃中, 袁蔚文. 南海北部底拖网渔业资源的数量变动[J]. 南方水产, 2008, 4(2): 26-33. doi: 10.3969/j.issn.2095-0780.2008.02.005
    [7]
    侯刚, 朱立新, 卢伙胜. 北部湾二长棘鲷生长、死亡及其群体组成[J]. 广东海洋大学学报, 2008, 28(3): 50-55. doi: 10.3969/j.issn.1673-9159.2008.03.011
    [8]
    冯波, 侯刚, 卢伙胜, 等. 北部湾二长棘鲷资源开发保护策略研究[J]. 农业系统科学与综合研究, 2009, 25(1): 114-119. doi: 10.3969/j.issn.1001-0068.2009.01.024
    [9]
    冯波, 徐翼, 卢伙胜. 北部湾二长棘鲷休渔效果模拟评价[J]. 资源科学, 2009, 31(12): 2201-2207. https://www.cqvip.com/QK/90831A/200912/32508677.html
    [10]
    朱立新, 李丽芳, 梁振林. 不确定性对北部湾二长棘鲷渔业资源评估影响的模拟研究[J]. 广东海洋大学学报, 2009, 29(1): 36-43. doi: 10.3969/j.issn.1673-9159.2009.01.008
    [11]
    王雪辉, 邱永松, 杜飞雁. 北部湾鱼类群落格局及其与环境因子的关系[J]. 水产学报, 2010, 34(10): 1580-1586. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg1zY3hiMjAxMDEwMDE1Ggh4aGhsOWg5bA%3D%3D
    [12]
    王雪辉, 邱永松, 杜飞雁, 等. 北部湾鱼类多样性及优势种的时空变化[J]. 中国水产科学, 2011, 18(2): 427-436. https://d.wanfangdata.com.cn/periodical/ChpNaW5lclBlcmlvZGljYWxDSEkyMDIyMTIwNhIPemdzY2t4MjAxMTAyMDIxGghjbm0zbTV4Zw%3D%3D
    [13]
    王学锋, 冯波, 侯刚. 基于EPR模型的北部湾二长棘鲷渔业管理研究[J]. 农业系统科学与综合研究, 2011, 27(4): 444-448. doi: 10.3969/j.issn.1001-0068.2011.04.012
    [14]
    王雪辉, 邱永松, 杜飞雁. 北部湾秋季底层鱼类多样性和优势种数量的变动趋势[J]. 生态学报, 2012, 32(2): 333-342. doi: 10.5846/stxb201011291700
    [15]
    孙典荣, 林昭进. 北部湾主要经济鱼类资源变动分析及保护对策探讨[J]. 热带海洋学报, 2004, 23(2): 62-68. doi: 10.3969/j.issn.1009-5470.2004.02.008
    [16]
    孙立元, 郭华阳, 朱彩艳, 等. 卵形鲳鲹育种群体遗传多样性分析[J]. 南方水产科学, 2014, 10(2): 67-71. doi: 10.3969/j.issn.2095-0780.2014.02.010
    [17]
    李莉好, 喻达辉. 基因组微卫星DNA位点的分离方法及其在水产动物中的应用[J]. 南方水产, 2006, 2(5): 74-80. doi: 10.3969/j.issn.2095-0780.2006.05.014
    [18]
    刘志毅, 相建海. 微卫星DNA分子标记在海洋动物遗传分析中的应用[J]. 海洋科学, 2001, 25(6): 11-13. doi: 10.3969/j.issn.1000-3096.2001.06.004
    [19]
    ROUSSET F. GENEPOP′007: a complete reimplementation of the GENEPOP software for Windows and Linux [J]. Mol Ecol Notes, 2007, 8(1): 103-106. doi: 10.1111/j.1471-8286.2007.01931.x
    [20]
    LIN Lin, ZHU Ling, LIU Shufang, et al. Polymorphic microsatellite loci for the Japanese anchovy Engraulis japonicas (Engraulidae) [J]. Genet Mol Res, 2011, 10(1): 764-768. doi: 10.4238/vol10-2gmr1085
    [21]
    LIN Lin, ZHU Ling, LIU Shufang, et al. Polymorphic microsatellite loci for Japanese Spanish mackerel (Scomberomorus niphonius) [J]. Genet Mol Res, 2012, 11(2): 1205-1208. doi: 10.4238/2012.May.8.2
    [22]
    LIU Shufang, LIU Hongbo, LIN Lin, et al. Development of 20 microsatellite markers for Solenocera crassicornis and their cross-species application in Solenocera melantho[J]. Int J Mol Sci, 2012, 13(7): 9218-9224. doi: 10.3390/ijms13079218
    [23]
    KONG Xiaolan, CHEN Zuozhi, LIN Lin, et al. Polymorphic microsatellite loci isolated from the yellowbelly threadfi bream, Nemipterus bathybius [J]. Genet Mol Res, 2014, 13(3): 5254-5257. doi: 10.4238/2014.July.24.3
    [24]
    LIN Lin, LI Chunhou, CHEN Zuozhi, et al. Development and characterization of twenty-three microsatellite markers for the purpleback flying squid (Symplectoteuthis oualaniensis) [J]. Conserv Genet Resour, 2015, 7(1): 161-163. doi: 10.1007/s12686-014-0318-1
    [25]
    秦海峰, 龙宁, 吴建国, 等. 甜叶菊微卫星富集文库的构建与多态性标记的筛选[J]. 作物学报, 2014, 40(3): 447-456. doi: 10.3724/SP.J.1006.2014.00447
    [26]
    TAKAGI M, TANIGUCHI N, COOK D, et al. Isolation and characterization of microsatellite loci from red sea bream Pagrus major and detection in closely related species [J]. Fish Sci, 1997, 63(2): 199-204. doi: 10.2331/fishsci.63.199
    [27]
    LAUNEY S, KRIEG F, HAFFRAY P, et al. Twelve new microsatellite markers for gilted seabream (Sparus aurata L. ): characterization, polymorphism and linkage [J]. Mol Ecol Notes, 2003, 3(3): 457-459. doi: 10.1046/j.1471-8286.2003.00482.x
    [28]
    LIU Lu, LIN Lin, LI Chunhou, et al. Polymorphic microsatellite loci for the crimson snapper (Lutjanus erythropterus) [J]. Genet Mol Res, 2014, 13(3): 5250-5253. doi: 10.4238/2014.July.24.2
    • Related Articles

  • Related Articles

    [1]ZHANG Wenwei, HUANG Zhong, DUAN Yafei, LIN Heizhao, JIANG Shigui, HUANG Jianhua. Effects of different disinfectants on water quality and microbial community structure of Litopenaeus vannamei[J]. South China Fisheries Science, 2021, 17(2): 1-10. DOI: 10.12131/20200236
    [2]ZHANG Jiachen, LIU Jianyong, YUAN Ruipeng, HU Zhiguo. Effect of stocking density on growth and survival of inbred and hybrid offspring of Litopenaeus vannamei[J]. South China Fisheries Science, 2015, 11(4): 53-58. DOI: 10.3969/j.issn.2095-0780.2015.04.008
    [3]LI Guolie, WANG Yuan, FANG Wenhong, SHEN Jinyu, ZHOU Junfang. Pharmacokinetics and bioavailability of sarafloxacin hydrochloride in Litopenaeus vannamei[J]. South China Fisheries Science, 2014, 10(1): 50-56. DOI: 10.3969/j.issn.2095-0780.2014.01.008
    [4]ZHANG Huajun, LI Zhuojia, ZHANG Jiasong, ZHANG Xiaoyang, CAO Yucheng, WEN Guoliang, CHENG Kaimin. Effects of stocking density on immune parameters and growth of juvenile Litopenaeus vannamei[J]. South China Fisheries Science, 2012, 8(4): 43-48. DOI: 10.3969/j.issn.2095-0780.2012.04.007
    [5]QIU Zefeng, ZHANG Liang, ZENG Weicai, JIAN Zhuoying, GAO Jialong, LIU Shucheng. Effect of frozen storage on muscle texture of Litopenaeus vannamei[J]. South China Fisheries Science, 2011, 7(5): 63-67. DOI: 10.3969/j.issn.2095-0780.2011.05.010
    [6]XIE Xiaoyong, SU Tianfeng, CHEN Wen, ZHANG Zhi, YAN Yuanyi, JIANG Shigui. Analysis on genetic diversity of six cultured stocks of Litopenaeus vannamei[J]. South China Fisheries Science, 2008, 4(6): 42-49.
    [7]LI Chunhou, QIN Honggui, JIA Xiaoping, TIAN Lixia. The effect of density on energy conversion efficiency of juvenile shrimp Litopenaeus vannamei[J]. South China Fisheries Science, 2006, 2(1): 30-33.
    [8]LIN Heizhe, YE Le, CHEN Yanjun, ZHOU Jinghui, LI Zhuojia, WU Kaichang. Effect of feed additive Stafac-500 on the growth and feed conversion for juvenile white shrimp, Litopenaeus vannamei[J]. South China Fisheries Science, 2005, 1(6): 63-65.
    [9]YE Le, LIN Hei-zhe, LI Zhuo-jia, WU Kai-chang, WEN Guo-liang, MA Zhi-ming, ZHU Chang-fu. The effect of feeding frequency on growth of Litopenaeus vannamei (Boone) and water quality[J]. South China Fisheries Science, 2005, 1(4): 55-59.
    [10]LI Zhuo-jia, LIN Liang, YANG Ying-ying, LIN Xiao-tao. Effect of Bacillus commercial probiotic on intestinal microflora of white shrimp, Litopenaeus vannamei[J]. South China Fisheries Science, 2005, 1(3): 54-59.

  • Cited by

    Periodical cited type(8)

    1. 朱轩仪，郑晓婷，邢逸夫，黄建华，董宏标，张家松. 三丁酸甘油酯提高凡纳滨对虾鳃组织抗周期性高温胁迫能力的研究. 南方水产科学. 2024(03): 66-75 . 本站查看
    2. 胡晓娟，杨铿，文国樑，苏浩昌，许云娜，徐创文，徐煜，徐武杰，曹煜成. 溶藻菌CZBC1在氯化物型盐碱水中对铜绿微囊藻的溶藻效果研究. 南方水产科学. 2024(05): 169-175 . 本站查看
    3. 江昀珈，胡晓娟，杨铿，曹煜成，许云娜，徐创文，张建设，文国樑. 蜡样芽孢杆菌TC-1对硫酸盐型盐碱水的生长适应性及其对微囊藻的溶藻效果. 中国水产科学. 2024(08): 954-965 .
    4. 曹煜成，孙志伟，徐煜，胡晓娟，苏浩昌，徐武杰，文国樑，鲁敏，余招龙. 三种微藻的生物量与其细胞氮磷碳的相互关系. 生态科学. 2022(04): 204-211 .
    5. 鲁敏，曹煜成，胡晓娟，许云娜，孙志伟，张建设，文国樑. 钝顶螺旋藻SP1(Spirulina platensis)对集约化养殖尾水氮磷的去除效果. 生态科学. 2021(02): 125-132 .
    6. 陈加雄，李伟滨，陈伟洲，马庆涛，陈楷亮. 封闭式虾池水体环境因子变化及浮游微藻优势种群演替规律. 湖北农业科学. 2018(11): 29-31+45 .
    7. 曹煜成，徐煜，黄小帅，胡晓娟，李卓佳，李凡，陈勇贵，文国樑. 铜绿微囊藻对WSSV潜伏感染对虾的致死效应. 南方农业学报. 2018(08): 1648-1653 .
    8. Jiaxiong CHEN，Weibin LI，Weizhou CHEN，Qingtao MA，Kailiang CHEN. Variation of Environmental Factors and Dominant Population Succession of Microalgae Planktonic in Closed Shrimp Pond. Agricultural Biotechnology. 2018(06): 188-191 .

    Other cited types(2)

Catalog

    Get Citation
    PDF
    XML
    Article views (3428) PDF downloads (1573) Cited by(10)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return