Volume 11 Issue 4
Aug.  2015
Turn off MathJax
Article Contents
Abstract
References
Related Articles
YANG Bing, LIN Lin, LI Chunhou, XU Shannan, LIU Yong, XIAO Yayuan, CHEN Zuozhi. Development and evaluation of microsatellite markers in Parargyrops edita[J]. South China Fisheries Science, 2015, 11(4): 116-120. DOI: 10.3969/j.issn.2095-0780.2015.04.017
Citation: YANG Bing, LIN Lin, LI Chunhou, XU Shannan, LIU Yong, XIAO Yayuan, CHEN Zuozhi. Development and evaluation of microsatellite markers in Parargyrops edita[J]. South China Fisheries Science, 2015, 11(4): 116-120. DOI: 10.3969/j.issn.2095-0780.2015.04.017
shu

Development and evaluation of microsatellite markers in Parargyrops edita

  • 1.

    Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

More Information
  • Received Date: March 24, 2015
  • Revised Date: April 19, 2015
    Graphical Abstract
    • Abstract

  • We isolated and characterized 15 polymorphic microsatellite markers in crimson seabream (Parargyrops edita) using high-throughput sequencing and polyacrylamide gel electrophoresis (PAGE), having obtained 0.12 Gbp spliced sequences of P.edita and searched 292 microsatellite sequences with suitable flanking regions from the spliced sequences. Then we designed 40 primer pairs in the microsatellite sequences for amplification by polymerase chain reaction (PCR), among which 15 primer pairs were clearly amplified and shown to be polymorphic. We found 2~16 alleles per locus (averagely 6.93). The observed and expected heterozygosities ranged from 0.268 to 0.979 and from 0.300 to 0.914, with an average of 0.568 and 0.640, respectively. No loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni correction, except for three loci. These markers will be useful for analyzing the population genetic diversity characteristics of P.edita.

    • FullText(HTML)
    • References (28)
  • [1]
    陈作志, 邱永松. 北部湾二长棘鲷的资源变动[J]. 南方水产, 2005, 1(3): 26-31. doi: 10.3969/j.issn.2095-0780.2005.03.004
    [2]
    赵传絪, 刘效顺, 曾炳光, 等. 中国海洋渔业资源[M]. 杭州: 浙江科学技术出版社, 1990: 55-56.
    [3]
    陈作志, 邱永松. 北部湾二长棘鲷生长和死亡参数估计[J]. 水产学报, 2003, 27(3): 251-257. doi: 10.3321/j.issn:1000-0615.2003.03.010
    [4]
    陈作志, 邱永松. 南海区海洋渔业资源现状和可持续利用对策[J]. 湖北农学院学报, 2002, 22(6): 507-510. https://xueshu.baidu.com/usercenter/paper/show?paperid=d158c197849ba1a8eb5f9f7adaba97cc&site=xueshu_se&hitarticle=1
    [5]
    陈作志, 邱永松. 北部湾二长棘鲷的生态分布[J]. 海洋水产研究, 2005, 26(3): 16-21. doi: 10.3969/j.issn.1000-7075.2005.03.003
    [6]
    王跃中, 袁蔚文. 南海北部底拖网渔业资源的数量变动[J]. 南方水产, 2008, 4(2): 26-33. doi: 10.3969/j.issn.2095-0780.2008.02.005
    [7]
    侯刚, 朱立新, 卢伙胜. 北部湾二长棘鲷生长、死亡及其群体组成[J]. 广东海洋大学学报, 2008, 28(3): 50-55. doi: 10.3969/j.issn.1673-9159.2008.03.011
    [8]
    冯波, 侯刚, 卢伙胜, 等. 北部湾二长棘鲷资源开发保护策略研究[J]. 农业系统科学与综合研究, 2009, 25(1): 114-119. doi: 10.3969/j.issn.1001-0068.2009.01.024
    [9]
    冯波, 徐翼, 卢伙胜. 北部湾二长棘鲷休渔效果模拟评价[J]. 资源科学, 2009, 31(12): 2201-2207. https://www.cqvip.com/QK/90831A/200912/32508677.html
    [10]
    朱立新, 李丽芳, 梁振林. 不确定性对北部湾二长棘鲷渔业资源评估影响的模拟研究[J]. 广东海洋大学学报, 2009, 29(1): 36-43. doi: 10.3969/j.issn.1673-9159.2009.01.008
    [11]
    王雪辉, 邱永松, 杜飞雁. 北部湾鱼类群落格局及其与环境因子的关系[J]. 水产学报, 2010, 34(10): 1580-1586. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg1zY3hiMjAxMDEwMDE1Ggh4aGhsOWg5bA%3D%3D
    [12]
    王雪辉, 邱永松, 杜飞雁, 等. 北部湾鱼类多样性及优势种的时空变化[J]. 中国水产科学, 2011, 18(2): 427-436. https://d.wanfangdata.com.cn/periodical/ChpNaW5lclBlcmlvZGljYWxDSEkyMDIyMTIwNhIPemdzY2t4MjAxMTAyMDIxGghjbm0zbTV4Zw%3D%3D
    [13]
    王学锋, 冯波, 侯刚. 基于EPR模型的北部湾二长棘鲷渔业管理研究[J]. 农业系统科学与综合研究, 2011, 27(4): 444-448. doi: 10.3969/j.issn.1001-0068.2011.04.012
    [14]
    王雪辉, 邱永松, 杜飞雁. 北部湾秋季底层鱼类多样性和优势种数量的变动趋势[J]. 生态学报, 2012, 32(2): 333-342. doi: 10.5846/stxb201011291700
    [15]
    孙典荣, 林昭进. 北部湾主要经济鱼类资源变动分析及保护对策探讨[J]. 热带海洋学报, 2004, 23(2): 62-68. doi: 10.3969/j.issn.1009-5470.2004.02.008
    [16]
    孙立元, 郭华阳, 朱彩艳, 等. 卵形鲳鲹育种群体遗传多样性分析[J]. 南方水产科学, 2014, 10(2): 67-71. doi: 10.3969/j.issn.2095-0780.2014.02.010
    [17]
    李莉好, 喻达辉. 基因组微卫星DNA位点的分离方法及其在水产动物中的应用[J]. 南方水产, 2006, 2(5): 74-80. doi: 10.3969/j.issn.2095-0780.2006.05.014
    [18]
    刘志毅, 相建海. 微卫星DNA分子标记在海洋动物遗传分析中的应用[J]. 海洋科学, 2001, 25(6): 11-13. doi: 10.3969/j.issn.1000-3096.2001.06.004
    [19]
    ROUSSET F. GENEPOP′007: a complete reimplementation of the GENEPOP software for Windows and Linux [J]. Mol Ecol Notes, 2007, 8(1): 103-106. doi: 10.1111/j.1471-8286.2007.01931.x
    [20]
    LIN Lin, ZHU Ling, LIU Shufang, et al. Polymorphic microsatellite loci for the Japanese anchovy Engraulis japonicas (Engraulidae) [J]. Genet Mol Res, 2011, 10(1): 764-768. doi: 10.4238/vol10-2gmr1085
    [21]
    LIN Lin, ZHU Ling, LIU Shufang, et al. Polymorphic microsatellite loci for Japanese Spanish mackerel (Scomberomorus niphonius) [J]. Genet Mol Res, 2012, 11(2): 1205-1208. doi: 10.4238/2012.May.8.2
    [22]
    LIU Shufang, LIU Hongbo, LIN Lin, et al. Development of 20 microsatellite markers for Solenocera crassicornis and their cross-species application in Solenocera melantho[J]. Int J Mol Sci, 2012, 13(7): 9218-9224. doi: 10.3390/ijms13079218
    [23]
    KONG Xiaolan, CHEN Zuozhi, LIN Lin, et al. Polymorphic microsatellite loci isolated from the yellowbelly threadfi bream, Nemipterus bathybius [J]. Genet Mol Res, 2014, 13(3): 5254-5257. doi: 10.4238/2014.July.24.3
    [24]
    LIN Lin, LI Chunhou, CHEN Zuozhi, et al. Development and characterization of twenty-three microsatellite markers for the purpleback flying squid (Symplectoteuthis oualaniensis) [J]. Conserv Genet Resour, 2015, 7(1): 161-163. doi: 10.1007/s12686-014-0318-1
    [25]
    秦海峰, 龙宁, 吴建国, 等. 甜叶菊微卫星富集文库的构建与多态性标记的筛选[J]. 作物学报, 2014, 40(3): 447-456. doi: 10.3724/SP.J.1006.2014.00447
    [26]
    TAKAGI M, TANIGUCHI N, COOK D, et al. Isolation and characterization of microsatellite loci from red sea bream Pagrus major and detection in closely related species [J]. Fish Sci, 1997, 63(2): 199-204. doi: 10.2331/fishsci.63.199
    [27]
    LAUNEY S, KRIEG F, HAFFRAY P, et al. Twelve new microsatellite markers for gilted seabream (Sparus aurata L. ): characterization, polymorphism and linkage [J]. Mol Ecol Notes, 2003, 3(3): 457-459. doi: 10.1046/j.1471-8286.2003.00482.x
    [28]
    LIU Lu, LIN Lin, LI Chunhou, et al. Polymorphic microsatellite loci for the crimson snapper (Lutjanus erythropterus) [J]. Genet Mol Res, 2014, 13(3): 5250-5253. doi: 10.4238/2014.July.24.2
    • Related Articles

  • Related Articles

    [1]GONG Yuyan, XIAO Yayuan, XU Shannan, LIU Yong, YANG Yutao, HUANG Zirong, LI Chunhou. Zooplankton community structure in Hailing Bay and its relationship with primary environmental factors[J]. South China Fisheries Science, 2019, 15(6): 49-55. DOI: 10.12131/20180220
    [2]LI Min, LI Yufang, ZHANG Peng, CHEN Zuozhi. Analysis of population genetic structure of bullet tuna(Auxis rochei) in the South China Sea based on mitochondrial control region sequences[J]. South China Fisheries Science, 2016, 12(4): 88-95. DOI: 10.3969/j.issn.2095-0780.2016.04.011
    [3]HU Yuting, HU Wang, LING Jun, JIANG He, DUAN Guoqing, PAN Tingshuang. Comparative and phylogenetic analysis of mitochondrial Cytochrome b gene and control region from natural triploid (Carassius auratus gibelio) in Chuzhou[J]. South China Fisheries Science, 2015, 11(2): 101-108. DOI: 10.3969/j.issn.2095-0780.2015.02.015
    [4]XIONG Dan, LI Min, CHEN Zuozhi, LI Yongzhen, LI Yufang, HUANG Zirong. Genetic structure of Priacanthus macracanthus population from the South China Sea[J]. South China Fisheries Science, 2015, 11(2): 27-34. DOI: 10.3969/j.issn.2095-0780.2015.02.004
    [5]RONG Chaozhen, ZU Guozhang, HU Jianhua, SUN Shouqi, SUN Tangli. Structure of mitochondrial DNA control region and genetic diversity of Misgurnus anguillicaudatus[J]. South China Fisheries Science, 2011, 7(5): 55-62. DOI: 10.3969/j.issn.2095-0780.2011.05.009
    [6]SU Tianfeng, JIANG Shigui. Structure and phylogenetic analysis of mitochondrial DNA control region of genus Trachurus[J]. South China Fisheries Science, 2011, 7(1): 18-25. DOI: 10.3969/j.issn.2095-0780.2011.01.003
    [7]CHEN Lei, CHENG Yuanzhi, WANG Rixin, XU Tianjun. Cloning and sequence analysis of Cyt b gene in 2 Gobiidae fishes[J]. South China Fisheries Science, 2010, 6(5): 43-49. DOI: 10.3969/j.issn.1673-2227.2010.05.007
    [8]MAO Yangli, CAI Houcai, LI Chengjiu, GAO Tianxiang. Molecular phylogenetics of genus Mytilus based on COI and 16S rRNA sequences[J]. South China Fisheries Science, 2010, 6(5): 27-36. DOI: 10.3969/j.issn.1673-2227.2010.05.005
    [9]SUN Xifu, GAO Tianxiang, DOU Cunyin, TANG Zhebin, LIU Jun. Sequence analysis of mitochondral Cyt b gene, D-loop and molecular phylogeny of Neophocaena phocaenoides[J]. South China Fisheries Science, 2009, 5(5): 24-31. DOI: 10.3969/j.issn.1673-2227.2009.05.005
    [10]ZHANG Dianchang, SHAO Yanqing, SU Tianfeng, JIANG Shigui. The sequence analysis of mitochondrial cytochrome b gene and molecular phylogeny of Parargyrops edita[J]. South China Fisheries Science, 2007, 3(2): 1-7.

  • Cited by

    Periodical cited type(17)

    1. 陈杰，陈子廷，赵甜，王方海，李广宏. 蝇蛆蛋白混合物对美洲鳗鲡非特异性免疫功能的影响. 水产科学. 2023(01): 39-47 .
    2. 郑金仙，周东生，魏山山，熊云凤，孔有琴，张易祥，赵建华，刘艳，戚常乐，丁志丽. 动植物蛋白对日本沼虾生长、肌肉组成、抗氧化、TOR信号通路及食欲调节相关基因表达的影响. 水产学报. 2022(10): 1801-1812 .
    3. 孙丽慧，张海琪，黄爱霞，李倩，蒋荣响，林锋. 饲料中添加蛹肽粉或蝇蛆粉对拟穴青蟹幼蟹生长、体组成和消化酶活性的影响. 上海海洋大学学报. 2022(04): 906-914 .
    4. 张玉栋，王巧利，申红，吴娜，王俊刚. 蝇蛆粉对腹泻小鼠肠道大肠杆菌数量及血清抗氧化酶和消化相关酶活性的影响. 动物营养学报. 2020(02): 890-897 .
    5. 曾胡龙，陶正国，程宗佳，李辉，林黑着，黄忠，黄倩倩，丁宏标. 辣椒碱在凡纳滨对虾饲料中的应用研究. 水产科技情报. 2020(02): 86-90 .
    6. 易新文，陈瑞爱，徐家华. 鸡肉粉替代鱼粉对卵形鲳鲹生长、饲料利用和抗氧化力的影响. 中国海洋大学学报(自然科学版). 2019(12): 17-24 .
    7. 胡俊茹，王国霞，黄文庆，赵红霞，莫文艳，黄燕华. 黑水虻幼虫粉替代鱼粉对凡纳滨对虾幼虾生长性能、体组成、血清生化指标和抗氧化能力的影响. 动物营养学报. 2019(11): 5292-5300 .
    8. 张月，段亚飞，董宏标，张家松. 聚β-羟基丁酸酯对凡纳滨对虾肝胰腺免疫和消化指标的影响. 南方水产科学. 2017(05): 78-84 . 本站查看
    9. 杨育凯，黄小林，林黑着，陈利雄，李涛，黄忠，虞为. 对虾幼体营养需求研究进展. 广东农业科学. 2015(18): 103-109 .
    10. 张号杰，郭爱红，秦江帆，李孝伟，付云娜. 昆虫蛋白的营养价值及其在动物生产中的应用. 中国饲料. 2015(03): 28-30+34 .
    11. 姚清华，郭清雄，林香信，陆鹏，颜孙安，苏德森，林虬. 羽毛肽粉作为菲律宾鳗鲡饲料蛋白源初探. 水产科学. 2014(01): 52-55 .
    12. 李华，李立军，黄卫，程宁宁，杨梅玲. 蝇蛆粉在水产饲料中的应用研究. 饲料工业. 2014(21): 70-73 .
    13. 姜松，范嗣刚，温为庚，王军红，黄桂菊，刘宝锁，张博，喻达辉. 微生态制剂对糙海参生长、消化酶活性及水质的影响. 海洋渔业. 2014(04): 335-341 .
    14. 姜松，王军红，范嗣刚，温为庚，黄桂菊，刘宝锁，张博，喻达辉. 添加光合细菌对糙海参幼苗培育阶段的影响研究. 南方水产科学. 2014(06): 78-82 . 本站查看
    15. 何亚丁，华雪铭，孔纯，吴钊，陈晓明，朱伟星，焦建刚，周志刚. 小球藻藻渣替代豆粕对凡纳滨对虾生长性能和氮磷排放的影响. 水产学报. 2014(09): 1538-1547 .
    16. 张号杰，郭爱红. 昆虫蛋白营养价值及在畜牧生产中的应用. 饲料博览. 2014(10): 23-27 .
    17. 刘黎，叶金云，吴成龙，张易祥，邵仙萍，姜志强，明建华，刘沛. 饲料中蝇蛆粉用量对青鱼生长、体成分和消化酶活力的影响. 上海海洋大学学报. 2014(01): 43-50 .

    Other cited types(18)

Catalog

    Get Citation
    PDF
    XML
    Article views (3428) PDF downloads (1573) Cited by(35)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return