Development and evaluation of microsatellite markers in Parargyrops edita

We isolated and characterized 15 polymorphic microsatellite markers in crimson seabream (Parargyrops edita) using high-throughput sequencing and polyacrylamide gel electrophoresis (PAGE), having obtained 0.12 Gbp spliced sequences of P.edita and searched 292 microsatellite sequences with suitable flanking regions from the spliced sequences. Then we designed 40 primer pairs in the microsatellite sequences for amplification by polymerase chain reaction (PCR), among which 15 primer pairs were clearly amplified and shown to be polymorphic. We found 2~16 alleles per locus (averagely 6.93). The observed and expected heterozygosities ranged from 0.268 to 0.979 and from 0.300 to 0.914,with an average of 0.568 and 0.640, respectively. No loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni correction, except for three loci. These markers will be useful for analyzing the population genetic diversity characteristics of P.edita.