Volume 11 Issue 4
Aug.  2015
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YANG Bing, LIN Lin, LI Chunhou, XU Shannan, LIU Yong, XIAO Yayuan, CHEN Zuozhi. Development and evaluation of microsatellite markers in Parargyrops edita[J]. South China Fisheries Science, 2015, 11(4): 116-120. DOI: 10.3969/j.issn.2095-0780.2015.04.017
Citation: YANG Bing, LIN Lin, LI Chunhou, XU Shannan, LIU Yong, XIAO Yayuan, CHEN Zuozhi. Development and evaluation of microsatellite markers in Parargyrops edita[J]. South China Fisheries Science, 2015, 11(4): 116-120. DOI: 10.3969/j.issn.2095-0780.2015.04.017
shu

Development and evaluation of microsatellite markers in Parargyrops edita

  • 1.

    Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

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  • Received Date: March 24, 2015
  • Revised Date: April 19, 2015
    Graphical Abstract
    • Abstract

  • We isolated and characterized 15 polymorphic microsatellite markers in crimson seabream (Parargyrops edita) using high-throughput sequencing and polyacrylamide gel electrophoresis (PAGE), having obtained 0.12 Gbp spliced sequences of P.edita and searched 292 microsatellite sequences with suitable flanking regions from the spliced sequences. Then we designed 40 primer pairs in the microsatellite sequences for amplification by polymerase chain reaction (PCR), among which 15 primer pairs were clearly amplified and shown to be polymorphic. We found 2~16 alleles per locus (averagely 6.93). The observed and expected heterozygosities ranged from 0.268 to 0.979 and from 0.300 to 0.914, with an average of 0.568 and 0.640, respectively. No loci showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni correction, except for three loci. These markers will be useful for analyzing the population genetic diversity characteristics of P.edita.

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    • References (28)
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