Loading [MathJax]/jax/output/SVG/jax.js
XIN Yanjie, HU Xiaojuan, CAO Yucheng, XU Yu, XU Yunna, SU Haochang, XU Chuangwen, WEN Guoliang, LI Zhuojia. Effects of inoculant of photosynthetic bacteria and Rhodopseudomonas palustris on nitrogen and phosphorus nutrients and microbial community in experimental water[J]. South China Fisheries Science, 2019, 15(1): 31-41. DOI: 10.12131/20180144
Citation: XIN Yanjie, HU Xiaojuan, CAO Yucheng, XU Yu, XU Yunna, SU Haochang, XU Chuangwen, WEN Guoliang, LI Zhuojia. Effects of inoculant of photosynthetic bacteria and Rhodopseudomonas palustris on nitrogen and phosphorus nutrients and microbial community in experimental water[J]. South China Fisheries Science, 2019, 15(1): 31-41. DOI: 10.12131/20180144

Effects of inoculant of photosynthetic bacteria and Rhodopseudomonas palustris on nitrogen and phosphorus nutrients and microbial community in experimental water

More Information
  • Received Date: June 26, 2018
  • Revised Date: September 17, 2018
  • Accepted Date: October 11, 2018
  • Available Online: December 09, 2018
  • To compare the physiological and ecological characteristics of inoculant of photosynthetic bacteria and Rhodopseudomonas palustri, we analyzed the degradation effects of the inoculant PG and the strain PSB-1 with different initial amounts of bacteria on ammonia nitrogen (

    \begin{document}${\rm NH}^+_4$\end{document}

    -N), nitrite nitrogen (

    NO2

    -N), nitrate nitrogen (

    NO3

    -N) and phosphate (

    PO34

    -P) in experimental water. Then we studied the dominant bacteria composition of inoculant of photosynthetic bacteria PG, as well as the number of bacteria and microbial community composition in experimental water at the end of the experiment by high-throughput sequencing. The results show that the inoculant PG group had certain degradation effects on

    PO34

    -P,

    NO3

    -N and

    NO2

    -N in experimental water. The maximum degradation rates of PG for

    PO34

    -P,

    NO3

    -N and

    NO2

    -N were 40.98%, 28.28% and 20.12%, respectively. Moreover, the strain PSB-1 group only had certain degradation effect on

    NO2

    -N and

    PO34

    -P. The maximum degradation rates of PSB-1 were 14.19% and 9.88%, respectively. The dominant bacteria of the inoculant PG belonged to Rhodopseudomonas sp.. After 7 d of experiment, the number of bacteria and microbial community structure in the experimental group changed, and the number of bacteria in the water increased, forming a microbial structure with heterotrophic bacteria as the dominant bacteria. It is indicated that the inoculant of photosynthetic bacteria PG has better effect on the degradation of water quality factors than R. palustris PSB-1. However, there is a certain gap between both things and the reported degradation efficiency of photosynthetic bacterial strains.

  • [1]
    李军, 张辉, 李建文, 等. 光合细菌菌剂在提高板蓝根品质中的应用研究[J]. 中国医药导报, 2011, 8(13): 58-59. doi: 10.3969/j.issn.1673-7210.2011.13.024
    [2]
    彭智平, 李文英, 徐培智, 等. 光合菌剂对菜心产量和品质的效应评价[J]. 广东农业科学, 2012, 39(1): 11-13. doi: 10.3969/j.issn.1004-874X.2012.01.004
    [3]
    BANEEJEE G, RAY A K. The advancement of probiotics research and its application in fish farming industries[J]. Rese Vet Sci, 2017, 115: 66-77. doi: 10.1016/j.rvsc.2017.01.016
    [4]
    FARZANFAR A. The use of probiotics in shrimp aquaculture[J]. Fems Immuno Med Microbiol, 2006, 48(2): 149-158. doi: 10.1111/j.1574-695X.2006.00116.x
    [5]
    JEONG S K, CHO J S, KONG I S, et al. Purification of aquarium water by PVA gel-immobilized photosynthetic bacteria during goldfish rearing[J]. Biotechnol Bioproc Engin, 2009, 14(2): 238-247. doi: 10.1007/s12257-008-0195-0
    [6]
    SHIUNG L L, CHANG M J, CHANG Y T, et al. Photosynthetic purple sulfur bacterium Marichromatium RuA2 induces changes in water quality parameters, the occurrence of sulfonamide resistance gene and microbial community structure of marine aquaculture[J]. Aquaculture, 2018, 493: 68-78. doi: 10.1016/j.aquaculture.2018.04.055
    [7]
    丁雷, 赵德炳. 光合细菌在水产养殖上的应用研究与进展[J]. 水利渔业, 2001, 21(1): 23-25. doi: 10.3969/j.issn.1003-1278.2001.01.011
    [8]
    KIM J K, LEE B K. Mass production of Rhodopseudomonas palustris as diet for aquaculture[J]. Aquacult Engin, 2000, 23(4): 281-293. doi: 10.1016/S0144-8609(00)00057-1
    [9]
    SHAPLEIGH J P. Dissimilatory and assimilatory nitrate reduction in the purple photosynthetic bacteria[M]. Dordrecht, Netherlands: Springer, 2009: 623-642.
    [10]
    王树香, 吴玉华, 杨炳武, 等. 淡水养殖净化菌株的筛选及其对水质的改善效果[J]. 贵州农业科学, 2014(3): 98-101. doi: 10.3969/j.issn.1001-3601.2014.03.024
    [11]
    杨官娥, 张肇铭, 赵利华, 等. 神奇的光合细菌菌剂[J]. 农产品加工, 2010(3): 8-9. doi: 10.3969/j.issn.1671-9646-C.2010.03.004
    [12]
    MUKHOPADHYAY M, CHAKRAVARTY D. Application of phototrophic purple non sulfur bacteria as fish feed for aquarium fishes[J]. J Environ Sociobiol, 2015(1): 21-21.
    [13]
    黄雪娇, 杨冲, 倪九派, 等. 1株高效去除氨氮的红假单胞菌的分离鉴定及特性[J]. 环境科学, 2016, 37(6): 2276-2283.
    [14]
    JIN H, ZHAO Y, LEI X C, et al. Treat shrimp wastewater with compound photosynthetic bacteria[C]//International Conference on Computer Distributed Control and Intelligent Environmental Monitoring (CDCIEM). Changsha: IEEE, 2011: 2352-2355.
    [15]
    冯亚丽. 海洋光合细菌在水产养殖中的应用基础研究[D]. 哈尔滨: 哈尔滨工业大学, 2017: 40-46.
    [16]
    布坎南 R E, 吉本斯N E. 伯杰氏细菌鉴定手册[M]. 8版. 北京: 科学出版社, 1984: 1-22.
    [17]
    郑卉. 一株光合细菌对养殖水水质调控及其在水体中的动态变化研究[D]. 宁波: 宁波大学, 2012: 15.
    [18]
    张晓波, 朱笔通, 熊慧, 等. 沼泽红假单胞菌(Rhodopseudomonas palustris) CQV97对无机三态氮共存水体中氮素的去除效率及其影响因素[J]. 氨基酸和生物资源, 2015, 37(4): 38-45.
    [19]
    文刚, 汪彬, 刘标, 等. 沼泽红假单胞菌R-3去除水体中氨氮的特性研究[J]. 湖南农业科学, 2017(6): 49-51.
    [20]
    陈燕红, 杨紫红, 喻国辉, 等. 光照、氧气、pH和盐度对沼泽红假单胞菌2-8菌株生长和亚硝酸盐消除的影响[J]. 南方水产, 2010, 6(4): 1-5. doi: 10.3969/j.issn.1673-2227.2010.04.001
    [21]
    雷爱莹, 彭敏, 曾地刚, 等. 高效净水沼泽红假单胞菌的分离和鉴定[J]. 广西农业科学, 2005, 36(1): 57-58. doi: 10.3969/j.issn.2095-1191.2005.01.025
    [22]
    袁翠霖. 罗非鱼养殖系统微生物群落生态研究及潜在有益菌的筛选[D]. 广州: 暨南大学, 2011: 50.
    [23]
    胡晓娟, 曹煜成, 文国樑, 等. 罗非鱼养殖系统潜在有机物降解菌的筛选及其降解能力测定[J]. 农业环境科学学报, 2014, 33(6): 1233-1239.
    [24]
    徐之文. 水质指标测定与分析及在水产养殖中的应用[J]. 吉林畜牧兽医, 2014, 35(5): 18-20. doi: 10.3969/j.issn.1672-2078.2014.05.004
    [25]
    李卓佳, 李奕雯, 曹煜成, 等. 凡纳滨对虾海水高位池养殖水体理化因子变化与营养状况分析[J]. 农业环境科学学报, 2010, 29(10): 2025-2032.
    [26]
    BRENNER D J, KRIEG N R, STALEY J T. Bergey's manual of systematic bacteriology[M]. 2th ed. New York: Springer, 2005: 1-474.
    [27]
    黄雪娇, 杨冲, 罗雅雪, 等. 光合细菌在水污染治理中的研究进展[J]. 中国生物工程杂志, 2014, 34(11): 119-124.
    [28]
    刘洋, 舒巧玉, 楼春燕, 等. 两株光合细菌的分离鉴定及其水质净化效果研究[J]. 淡水渔业, 2013, 43(5): 57-61. doi: 10.3969/j.issn.1000-6907.2013.05.010
    [29]
    秦宇胜, 张君枝, 马文林, 等. 北京地表水体光合细菌生长及脱氮除磷特性研究[J]. 环境工程, 2015(s1): 291-294.
    [30]
    董艳珍, 亓东明. 一株光合细菌的净水能力分析[J]. 西昌学院学报(自然科学版), 2013(3): 13-15. doi: 10.3969/j.issn.1673-1891.2013.03.006
    [31]
    ZHANG J, ZHANG W X, LI S Z, et al. A two-step fermentation of distillers' grains using Trichoderma viride and Rhodopseudomonas palustris for fish feed[J]. Bioprocess Biosyst Eng, 2013, 36(10): 1435-1443. doi: 10.1007/s00449-013-0887-5
    [32]
    ZHOU X X, PAN Y J, WANG Y B, et al. In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaeroides[J]. J Zhejiang Univ Sci B, 2007, 8(9): 686-692. doi: 10.1631/jzus.2007.B0686
    [33]
    QI Z Z, ZHANG X H, BOON N, et al. Probiotics in aquaculture of China: current state, problems and prospect[J]. Aquaculture, 2009, 290(1/2): 15-21.
    [34]
    林启存, 朱丽敏, 沈小红, 等. 一株光合细菌的分离与鉴定[J]. 浙江农业科学, 2017, 58(8): 1444-1446.
    [35]
    ZHANG X, SHU M, WANG Y, et al. Effect of photosynthetic bacteria on water quality and microbiota in grass carp culture[J]. World J Microbiol Biotechnol, 2014, 30(9): 2523-2531. doi: 10.1007/s11274-014-1677-1
    [36]
    杨莺莺, 曹煜成, 李卓佳, 等. PS1沼泽红假单胞菌对集约化对虾养殖废水的净化作用[J]. 中国微生态学杂志, 2009, 21(1): 4-6.
    [37]
    李莎莎, 曹煜成, 胡晓娟, 等. 响应面法优化芽孢杆菌(Bacillus sp.) A4的培养参数[J]. 南方水产科学, 2017, 13(5): 85-93. doi: 10.3969/j.issn.2095-0780.2017.05.012
    [38]
    张修建. 光合细菌制剂在水产养殖应用中需注意的问题[J]. 河北渔业, 2008(11): 8-9. doi: 10.3969/j.issn.1004-6755.2008.11.003
  • Related Articles

    [1]SONG Yuqiong, HU Xiao, XIANG Huan, CHEN Shengjun, LI Laihao, YANG Xianqing, WU Yanyan, MA Haixia. Study on tyrosinase inhibitory activity and Cu2+ binding activity of tilapia skin peptides[J]. South China Fisheries Science, 2023, 19(1): 155-164. DOI: 10.12131/20220097
    [2]HU Xiao, LIU Jing, GAO Ying, LI Ruijie, LI Laihao, YANG Xianqing, CHEN Shengjun, WU Yanyan, QI Bo, RONG Hui. Preparation and antioxidant properties of Maillard reaction products from Schizochytrium limacinum protein peptides[J]. South China Fisheries Science, 2021, 17(4): 58-65. DOI: 10.12131/20210056
    [3]YU Wei, OUYANG Tong, LUO Biqi, WU Bin, ZHENG Huina, CAO Wenhong, LIN Haisheng, ZHANG Chaohua, QIN Xiaoming. Study on extraction and purification of glycoprotein from Pinctada martensii' mucus and its antioxidant activity[J]. South China Fisheries Science, 2020, 16(4): 100-107. DOI: 10.12131/20200027
    [4]CAI Miaomiao, CHEN Shengjun, YANG Xianqing, MA Haixia, DENG Jianchao, LI Chunsheng, HU Xiao, QI Bo. Extraction and antioxidant activity of protein from Grateloupia livida[J]. South China Fisheries Science, 2020, 16(2): 99-106. DOI: 10.12131/20190232
    [5]HUANG Haichao, WANG Jinxu, PAN Chuang, YANG Xianqing, QI Bo, LI Laihao, ZHAO Yongqiang. Degradation of Porphyra haitanensis polysaccharide by ultrasonic assisted hydrogen peroxide method and its antioxidant activity analysis[J]. South China Fisheries Science, 2020, 16(1): 110-119. DOI: 10.12131/20190220
    [6]QIU Chaoying, RONG Jing, HU Xiao, YANG Xianqing, LI Laihao, HUANG Hui. Antioxidant properties of simulated in vitro digests of glycosylated Symplectoteuthis oualaniensis myofibrillar protein[J]. South China Fisheries Science, 2018, 14(6): 105-112. DOI: 10.12131/20180074
    [7]WANG Yueqi, LI Laihao, CAI Qiuxing, WU Yanyan, WANG Xichang, YANG Xianqing, ZHAO Yongqiang. Screening and identification of antioxidant starter culture strains from salted dried fish[J]. South China Fisheries Science, 2016, 12(3): 74-83. DOI: 10.3969/j.issn.2095-0780.2016.03.010
    [8]ZHANG Feng, XIA Xu, ZHOU Aimei, LIU Xin, CAO Yong. Research of antioxidant peptides produced from protein extracted from white shrimp head and shell[J]. South China Fisheries Science, 2015, 11(6): 79-87. DOI: 10.3969/j.issn.2095-0780.2015.06.011
    [9]CAO Rong, LI Dongyan, LIU Qi, ZHAO Ling, YIN Bangzhong. Antioxidation effects of polypeptides from intestines and gonads of sea cucumber (Apostichopus japonicus) by enzymatic hydrolyzation[J]. South China Fisheries Science, 2013, 9(6): 47-51. DOI: 10.3969/j.issn.2095-0780.2013.06.008
    [10]LIU Qiufeng, WU Chengye, SU Yongchang. Study on antioxidation, animal experiments of hyperglycemia and hyperlipemia of agaropectin from Gracilaria lemaneiformis[J]. South China Fisheries Science, 2013, 9(3): 57-66. DOI: 10.3969/j.issn.2095-0780.2013.03.010

Catalog

    Recommendations
    不同脱腥方法对薛氏海龙肽粉的脱腥效果比较
    陈茂森 et al., 南方水产科学, 2025
    尖翅燕鱼早期色素细胞发育与体色变化
    庾楚霞 et al., 南方水产科学, 2025
    牡蛎养殖对大鹏澳环境因子与浮游菌落扰动研究
    佟飞 et al., 南方水产科学, 2024
    溶藻菌czbc1在氯化物型盐碱水中对铜绿微囊藻的溶藻效果研究
    胡晓娟 et al., 南方水产科学, 2024
    螺旋藻粉对面团性质及面条品质的影响
    ZHANG Qingqing et al., JOURNAL OF HENAN UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE EDITION), 2024
    牛磺酸对大口黑鲈生长、糖脂代谢和抗氧化能力的影响
    边延欣 et al., 水产学报, 2025
    Impact of antibiotic pollution on the bacterial population within surface water with special focus on mountain rivers
    Kulik, Klaudia et al., WATER, 2023
    Early estimates of bivalent mrna booster dose vaccine effectiveness in preventing symptomatic sars-cov-2 infection attributable to omicron ba.5-and xbb/xbb.1.5-related sublineages among immunocompetent adults-increasing community access to testing program
    Link-Gelles, Ruth et al., MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT, 2023
    Effects of litsea cubeba essential oil on growth performance, blood antioxidation, immune function, apparent digestibility of nutrients, and fecal microflora of pigs
    FRONTIERS IN PHARMACOLOGY
    Pharmacodynamic study of redujing oral liquid on epstein-barr virus (ebv) associated infectious mononucleosis in children
    INTERNATIONAL JOURNAL OF PHARMACOLOGY, 2023
    Powered by
    Article views (5092) PDF downloads (99) Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return