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CHEN Xiaohong, SHEN Xiaona, XU Yibin, XU Cuiya, ZHENG Huidong. Analysis of tetrodotoxin and microbial community structure in Nassarius semiplicatus[J]. South China Fisheries Science. DOI: 10.12131/20240235
Citation: CHEN Xiaohong, SHEN Xiaona, XU Yibin, XU Cuiya, ZHENG Huidong. Analysis of tetrodotoxin and microbial community structure in Nassarius semiplicatus[J]. South China Fisheries Science. DOI: 10.12131/20240235
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Analysis of tetrodotoxin and microbial community structure in Nassarius semiplicatus

  • 1.

    Fujian Fishery Research Institute/Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Xiamen 361013, China

  • 2.

    College of Life Sciences, Fujian Normal University, Fuzhou 350117, China

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  • Received Date: October 07, 2024
  • Revised Date: December 23, 2024
  • Accepted Date: January 17, 2025
  • Available Online: March 02, 2025
    Graphical Abstract
    • Abstract

  • Tetrodotoxin (TTX) is a potent marine toxin, found in a great variety of marine and some terrestrial species. In order to explore the potential bacterial sources of tetrodotoxin (TTX) in Nassarius semiplicatus, we collected its TTX and analogues from Fujian and analyzed them by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Besides, we compared the microbial communities of TTX-bearing and non-TTX-bearing by using high-throughput sequencing technology. The results show that the content of TTX in TTX-bearing N. semiplicatus (LTN) were 402~644 μg·kg−1. TrideoxyTTX, dideoxyTTX and deoxyTTX were detected. TTX and other analogues were not detected in non-TTX-bearing N. semiplicatus (NTN) except dideoxyTTX. The results of high-throughput sequencing show that the community richness of LTN was significantly higher than that of NTN (p<0.05). The microbial communities of LTN and NTN were dominated by Proteobacteria. TTX-producing bacteria, including Vibrio, Pseudoalteromonas and Psychrobacter, were the dominant bacteria in LTN, while Pseudoalteromonas and Shewanella in NTN. The significant difference analysis shows that the relative abundance of 17 differential genera in LTN, including Vibrio and Psychrobacter, etc., were significantly higher than that of NTN (p<0.05). The KEGG functional composition of the intestinal flora of LTN and NTN were similar. The main metabolic pathways were amino acid metabolism, carbohydrate metabolism and so on. The results show that TTX-producing bacteria can exist in both LTN and NTN; Vibrio and Psychrobacteraremore enriched in LTN. These TTX-producing bacteria may be related to the biosynthesis of TTX analogues and precursors in N. semiplicatus.

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