Volume 18 Issue 5
Oct.  2022
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ZOU Cuiyun, CHEN Xiaojing, WU Yingxin, HUANG Jinxiong, TAN Xiaohong, HU Xinyue, GAN Songyong, WU Jinhui. Protective effect of Xiaochaihu Decoction on D-GalN/ LPS-induced hepatocyte injury in hybrid grouper (Epinephelus lanceolatusy♂× E. fuscoguttatus♀)[J]. South China Fisheries Science, 2022, 18(5): 66-73. DOI: 10.12131/20210362
Citation: ZOU Cuiyun, CHEN Xiaojing, WU Yingxin, HUANG Jinxiong, TAN Xiaohong, HU Xinyue, GAN Songyong, WU Jinhui. Protective effect of Xiaochaihu Decoction on D-GalN/ LPS-induced hepatocyte injury in hybrid grouper (Epinephelus lanceolatusy♂× E. fuscoguttatus♀)[J]. South China Fisheries Science, 2022, 18(5): 66-73. DOI: 10.12131/20210362
shu

Protective effect of Xiaochaihu Decoction on D-GalN/ LPS-induced hepatocyte injury in hybrid grouper (Epinephelus lanceolatusy♂× E. fuscoguttatus♀)

  • 1.

    College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering/Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center/Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, China

  • 2.

    Guangdong Provincial Agricultural Technology Extension Center, Huizhou 516081, China

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  • Received Date: November 30, 2021
  • Revised Date: January 30, 2022
  • Accepted Date: March 14, 2022
  • Available Online: March 29, 2022
    Graphical Abstract
    • Abstract
  • The study aims to evaluate the protective effect of Xiaochaihu Decoction (XCHD) on chemical hepatocyte injury. We treated the hepatocytes with D-GalN (20 mmol·L–1) and LPS (1 μg·mL–1) for 24 h to build a hybrid grouper (Epinephelus lanceolatus♂×E. fuscoguttatus♀) hepatocyte injury model, then pretreated the liver cells with different concentrations of XCHD (100, 200 and 400 μg·mL–1) (D-GalN/LPS damage), and measured the hepatocyte viability by the CCK-8 assay. Hepatocyte morphology was visualized by HE staining, and cell supernatants were collected to detect the content of the inflammatory factors INF-, COX-2, and PGE2, as well as the expression of apoptotic and immune-related genes in the cell cryoprecipitate. The results show that XCHD can improve the survival rate of hepatocytes, inhibit INF-γ activity and coX-2 and PGE2 contents in the supernatant of hepatocytes induced by D-GalN/LPS injury to a different extent, reduce LDH, AST and ALT contents and apoptosis rate of hepatocytes. Besides, it had a significant protective effect on the structure of hepatocytes, significantly inhibiting the up-regulation of apoptosis-related genes caspase-3, caspase-9 and P53, and enhancing the up-regulation of immune-related genes TLR3. According to the data from the statistical analysis, the inhibition effect was most obvious in the 200 μg·mL–1 XCHD group. In conclusion, XCHD has a protective effect on the chemical hepatocyte injury of Epinephelus lanceolatus♂×E. fuscoguttatus♀, and can prevent the occurrence and development of liver disease, which provides a new insight for the study of liver protection drugs in fish.
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    • References (30)
  • [1]
    张林宝, 陈海刚, 田斐, 等. 三唑磷对翡翠贻贝神经毒性与氧化损伤效应的性别差异研究[J]. 南方水产科学, 2020, 16(6): 75-80.
    [2]
    JUNIOR G B, de SOUZA C F, da SILVA H N P, et al. Combined effect of florfenicol with linalool via bath in combating Aeromonas hydrophila infection in silver catfish (Rhamdia quelen)[J]. Aquaculture, 2021, 545: 737247. doi: 10.1016/j.aquaculture.2021.737247
    [3]
    ARNOLD H, PLUTA H J, BRAUNBECK T. Simultaneous exposure of fish to endosulfan and disulfoton in vivo: ultrastructural, stereological and biochemical reactions in hepatocytes of male rainbow trout (Oncorhynchus mykiss)[J]. Aquat Toxicol, 1995, 33(1): 17-43. doi: 10.1016/0166-445X(95)00006-P
    [4]
    ZHENG T, JIA R, CAO L, et al. Alleviative effects of Ginkgo biloba extract on oxidative stress, inflammatory response and immune suppression induced by long-term glyphosate exposure in tilapia (Oreochromis niloticus)[J]. Aquaculture, 2022, 546: 737325. doi: 10.1016/j.aquaculture.2021.737325
    [5]
    ZHENG T, JIA R, CAO L, et al. Effects of chronic glyphosate exposure on antioxdative status, metabolism and immune response in tilapia (GIFT, Oreochromis niloticus)[J]. Comp Biochem Physiol C, 2020, 239: 108878.
    [6]
    SINGLEMAN C, HOLTZMAN N G. PCB and TCDD derived embryonic cardiac defects result from a novel AhR pathway[J]. Aquat Toxicol, 2021, 233: 105794. doi: 10.1016/j.aquatox.2021.105794
    [7]
    FU D, HU Y, CHU P, et al. Histopathological and calreticulin changes in the liver and gill of Takifugu fasciatus demonstrate the effects of copper nanoparticle and copper sulphate exposure[J]. Aquac Rep, 2021, 20(126): 100662.
    [8]
    AUTHMAN M M N, ABBAS W T, ABBAS H H, et al. Ameliorative effect of the dietary Egyptian leek (Allium ampeloprasum L. var. Kurrat) on zinc toxicity of the African catfish Clarias gariepinus (Burchell, 1822)[J]. Aquac Res, 2021, 52(11): 5656-5672. doi: 10.1111/are.15440
    [9]
    AZEVEDO A C B, BOZZA D A, DORIA H B, et al. Low levels of inorganic copper impair reproduction parameters in Oreochromis niloticus after chronic exposure[J]. Aquaculture, 2021, 545: 737186. doi: 10.1016/j.aquaculture.2021.737186
    [10]
    翟亚南, 王晶晶, 李梦, 等. D-氨基半乳糖联合脂多糖诱导小鼠慢性肝损伤模型的建立[J]. 中国比较医学杂志, 2014, 24(5): 62-65. doi: 10.3969/j.issn.1671-7856.2014.05.013
    [11]
    LIU B, LU Y, CHEN X, et al. Protective role of Shiitake mushroom-derived exosome-like nanoparticles in d-galactosamine and lipopolysaccharide-induced acute liver injury in mice[J]. Nutrients, 2020, 12(2): 477. doi: 10.3390/nu12020477
    [12]
    徐安乐, 黎中宝, 上官静波, 等. 复方中草药对珍珠龙胆石斑鱼生长、非特异性免疫及消化酶活性的影响[J]. 海洋学报, 2018, 40(12): 49-57.
    [13]
    黄景军, 何琪, 赵会宏, 等. 饲料中添加复方中草药对鞍带石斑鱼生长与消化酶活性的影响[J]. 饲料工业, 2021, 42(4): 53-58.
    [14]
    GAO S, WANG T, HUANG X K, et al. Exploring the protective effect of modified Xiaochaihu Decoction against hepatic steatosis and inflammation by network pharmacology and validation in ageing rats[J]. Chin Med, 2020, 15(1): 1-19. doi: 10.1186/s13020-019-0281-6
    [15]
    ZHU W, LUO H, XIONG M, et al. Xiaochaihu Decoction for nonalcoholic fatty liver disease: a protocol for a systematic review and meta-analysis[J]. Medicine, 2021, 100(12): e25267. doi: 10.1097/MD.0000000000025267
    [16]
    詹理辉, 黄古叶, 韦翠婷, 等. 经方治疗肝纤维化的研究进展[J]. 中医药临床杂志, 2021, 33(7): 1406-1409.
    [17]
    张志雄, 刘春芳, 刘明洋, 等. 小柴胡汤的药理作用及临床应用研究进展[J]. 中医药临床杂志, 2021, 33(3): 580-584.
    [18]
    ZOU C, XU M, CHEN L, et al. Xiaochaihu Decoction reduces hepatic steatosis and improves D-GalN/LPS-induced liver injury in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)[J]. Fish Shellfish Immunol, 2019, 91: 293-305. doi: 10.1016/j.fsi.2019.05.025
    [19]
    TAN X, SUN Z, LIU Q, et al. Effects of dietary ginkgo biloba leaf extract on growth performance, plasma biochemical parameters, fish composition, immune responses, liver histology, and immune and apoptosis-related genes expression of hybrid grouper (Epinephelus lanceolatus♂× Epinephelus fuscoguttatus♀) fed high lipid diets[J]. Fish Shellfish Immunol, 2018, 72: 399-409. doi: 10.1016/j.fsi.2017.10.022
    [20]
    郭楠, 郑怀东, 刘学光. 孔雀石绿在不同品种鱼类中残留量的研究与分析[J]. 河南水产, 2014(2): 29-30.
    [21]
    OGUNWOLE G A, ABIYA S E, AMAEZE N H, et al. Antioxidant markers in gills, liver and muscle tissue of the African sharptooth catfish (Clarias gariepinus) exposed to subchronic levels of Ibuprofen and Dibutyl phthalate[J]. Sci Afr, 2021, 12(12): e00816.
    [22]
    FU Q, SCHEIDEGGER A, LACZKO E, et al. Metabolomic profiling and toxicokinetics modeling to assess the effects of the pharmaceutical diclofenac in the aquatic invertebrate Hyalella azteca[J]. Environ Sci Technol, 2021, 55: 7920-7929. doi: 10.1021/acs.est.0c07887
    [23]
    翟雪珍. 小柴胡汤加减联合恩替卡韦治疗慢性乙型病毒性肝炎肝郁脾虚型疗效观察[J]. 实用中医药杂志, 2020, 36(1): 39-40.
    [24]
    王丽敏, 张丽艳, 谷松. 解析小柴胡汤“和法”调节自身免疫性疾病机制[J]. 中华中医药学刊, 2018, 36(1): 218-221.
    [25]
    AMADI C N, ORISAKWE O E. Herb-induced liver injuries in developing nations: an update[J]. Toxics, 2018, 6(2): 24. doi: 10.3390/toxics6020024
    [26]
    蔡乐. 基于肝器官芯片的中草药成分肝毒性评价[D]. 大连: 大连理工大学, 2019: 13-14.
    [27]
    商海涛, 庞欣欣, 张晴晴, 等. 中草药致肾损伤及其相关成分毒性机制研究进展[J]. 医药导报, 2021, 40(9): 1210-1215.
    [28]
    焦豪妍, 许梦怡, 师大智, 等. 蓝莓叶总黄酮对LPS/D-GalN诱导小鼠急性肝损伤的保护作用[J]. 食品研究与开发, 2020, 41(17): 1-6.
    [29]
    ZHANG T, CHEN Y, ZHAN Z, et al. Oridonin alleviates d-Galn/lps-induced acute liver injury by inhibiting NLRP3 inflammasome[J]. Drug Dev Res, 2021, 82(4): 575-580. doi: 10.1002/ddr.21776
    [30]
    TSUKAYAMA I, MEGA T, HOJO N, et al. Diosgenin suppresses COX-2 and mPGES-1 via GR and improves LPS-induced liver injury in mouse[J]. Prostag Oth Lipid M, 2021, 156: 106580.
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