Volume 16 Issue 2
Apr.  2020
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ZHONG Jiajia, ZHANG Chaohua, GAO Jialong, QIN Xiaoming, CAO Wenhong, ZHENG Huina, LIN Haisheng. Anti-hepatic injury effect of enzymatic hydrolysate from soft tissue of Pinctada martensii[J]. South China Fisheries Science, 2020, 16(2): 107-114. DOI: 10.12131/20190239
Citation: ZHONG Jiajia, ZHANG Chaohua, GAO Jialong, QIN Xiaoming, CAO Wenhong, ZHENG Huina, LIN Haisheng. Anti-hepatic injury effect of enzymatic hydrolysate from soft tissue of Pinctada martensii[J]. South China Fisheries Science, 2020, 16(2): 107-114. DOI: 10.12131/20190239
shu

Anti-hepatic injury effect of enzymatic hydrolysate from soft tissue of Pinctada martensii

  • 1.

    College of Food Science and Technology, Guangdong Ocean University/National Research and Development Branch Center for Shellfish Processing (Zhanjiang)/Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Guangdong Province Engineering Laboratory for Marine Biological Products/Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China

  • 2.

    Dalian Polytechnic University/Collaborative Innovation Center of Seafood Deep Processing, Dalian 116034, China

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  • Received Date: November 22, 2019
  • Revised Date: January 16, 2020
  • Accepted Date: February 14, 2020
  • Available Online: March 12, 2020
    Graphical Abstract
    • Abstract

  • In order to analyze the anti-hepatic injury effect of enzymatic hydrolysate from Pinctada martensii (EP), we seperated EP by membrane ultrafiltration into three molecular size fractions [MW> 10 kD (EP-Ⅰ), MW＝3–10 kD (EP-Ⅱ) and MW < 3 kD (EP-Ⅲ)], and then measured the effects of three ultrafiltration fractions on the anti-ALD in vitro and liver-protection on mice. The results of experiments in vitro show that EP-III could activate alcohol dehydrogenase (ADH) significantly (P<0.01), and="" three="" fractions="" demonstrated="" different="" antioxidant="" capacities="">EP-Ⅱ>EP-Ⅰ). The results of mice experiment show that compared with the alcohol model group, the activities of ALT and AST in serum, liver index, the levels of MDA and TG in liver decreased in each ultrafiltration fraction group significantly, while the activities of SOD, ADH and ALDH and the levels of GSH in liver increased significantly. Therefore, the ultrafiltration fractions of enzymatic hydrolysate from soft tissue of P. martensii showed good protective effect on alcoholic liver damage, and the effect of EP-Ⅲ with low molecular mass was the best. Its mechanism may be related to accelerating ethanol metabolism and slowing down the oxidative damage caused by ethanol.

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