YANG Yingying, LI Zhuojia, CHEN Yongqing, YANG Keng, WEN Guoliang, DING Xian. Fermentation technology of probiotic strain D-1[J]. South China Fisheries Science, 2005, 1(6): 44-49.
Citation: YANG Yingying, LI Zhuojia, CHEN Yongqing, YANG Keng, WEN Guoliang, DING Xian. Fermentation technology of probiotic strain D-1[J]. South China Fisheries Science, 2005, 1(6): 44-49.

Fermentation technology of probiotic strain D-1

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  • Received Date: August 23, 2005
  • Revised Date: September 15, 2005
  • In order to increase bacteria production and ratio of spore formation, experiments were conducted to obtain the best fermentation condition and media, and to determine the growth curve under the most favourable culture. The most favourable fermantation for strain D-1 was 30℃ in temperature, 6.5~6.8 in initial of media pH, 5% inoculum size, and 100 mL media per 500 mL flask in volume. Suitable culture medium was obtained through testing the single effect of medium elements on growth of D-1, including carbon sources, nitrogen sources, inorganic salt and growth factor.The optimal prescription for D-1 fermentation, as determined by orthogonal testing, was composed of 2% maize starch, 2.5% peptone, 0.8% NaH2PO4, 1.5% maize syrup, 0.05% MgSO4 · 7H2O and 0.1%MnSO4(3.08%). To obtain the best inoculate and culture time, the growth curve of D-1 was determined under the most favourable medium and culture conditions. As determined from the growth curve, the best inoculate time should be 18~20 h, and culture time should be 26 h.

  • [1]
    王丽娟. 益生菌的研究及其进展[J]. 饲料博览, 1999, 11(1): 15-18.
    [2]
    杨莺莺, 张庆, 李卓佳, 等. 有益微生物菌株环境耐受性试验[A]. 海洋水产科学研究文集[C]. 广州: 广东科技出版社, 2000.177-180. https://xueshu.baidu.com/usercenter/paper/show?paperid=5122ce9f001e01f942dab53431e0a48c&site=xueshu_se
    [3]
    胡东兴, 潘康成. 微生物制剂及其作用机理[J]. 中国饲料, 2001, 191(3): 14-16. doi: 10.3969/j.issn.1004-3314.2001.03.008
    [4]
    仇丽, 凌爱珍, 祝井爱. 枯草芽孢杆菌制剂改良中华绒螯蟹人工育苗水质的试验[J]. 中国水产, 2001, (4): 82. doi: 10.3969/j.issn.1002-6681.2001.04.051
    [5]
    丁雷, 岳永生, 李贵杰, 等. 芽孢菌对养鱼水质影响的研究[J]. 淡水渔业, 1999, 29(10): 7-10.
    [6]
    Gatesoupe F J. The use probiotics in aquaculture[J]. Aquac, 1999, 180(1-2): 147-165. doi: 10.1016/S0044-8486(99)00187-8
    [7]
    李琴, 董在杰. 芽孢杆菌及其在水产养殖中的应用[J]. 科学养鱼, 2004(8): 44-45.
    [8]
    周德庆. 微生物学实验手册[M]. 上海: 上海科学技术出版社, 1986.78-85. https://xueshu.baidu.com/usercenter/paper/show?paperid=ff7d405e6273222ccdee32a5ed014229&site=xueshu_se&hitarticle=1
    [9]
    上海市科学技术交流站组. 正交实验设计法[M]. 上海: 上海人民出版社, 1975.45-54. https://www.las.ac.cn/front/book/detail?id=aa9df246d276bd624750aafa25761e25

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