D-loop gene sequence and diversity of Leuciscus waleckii Dybowski in Dali Lake and Ganggeng Lake in Inner Mongolia

We amplified the mtDNA D-loop sequence intwo stocks of Leuciscus waleckii Dybowski in Dali Lake and Ganggeng Lake in Inner Mongolia, havingobtained896 bp gene sequences; the average contents of T, C, A and G of homological fragments were 32.0%, 32.2%, 21.3%, 14.5% and 32.0%, 32.2%, 21.4%, 14.5% in Dali Lake stock and Ganggeng Lake stock, respectively, with no significant difference. The inter- and intra-population of thetwo stocks had high degree of sequence polymorphism. There were 33 variable sites in 896 D-loop gene loci in thetwo stocks, and the rest sites were more conservative.The D-loop genes of 30 fish in Dali Lake contained 21 polymorphisms/mutations and 11 haplotypes; while those in Ganggeng Lake contained 31 polymorphisms /mutations and 17 haplotypes. The diversity of D-loop gene in Ganggeng Lake stock was higher than that in Dali Lake stock. Genetic distance calculation shows that the average genetic distance between Ganggeng Lake and Dali Lake stocks was 0.007 0(Dali Lake: 0.003 6, Ganggeng Lake: 0.007 4). The genetic distance of the D-loop gene in Ganggeng Lake stock was greater than that in Dali Lake stock, and the evolution speed of the former was fasterthat of than the latter.