Volume 19 Issue 1
Feb.  2023
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WEI Zhengkun, DONG Hongbiao, ZHAO Wen, CHEN Fei, ZHANG Chuanxiang, CHEN Jian, GONG Baohua, ZHU Changbo, ZHANG Jiasong. Anesthetic effect and tissue oxidative injury for Litopenaeus vannamei by two anesthetics[J]. South China Fisheries Science, 2023, 19(1): 136-146. DOI: 10.12131/20220070
Citation: WEI Zhengkun, DONG Hongbiao, ZHAO Wen, CHEN Fei, ZHANG Chuanxiang, CHEN Jian, GONG Baohua, ZHU Changbo, ZHANG Jiasong. Anesthetic effect and tissue oxidative injury for Litopenaeus vannamei by two anesthetics[J]. South China Fisheries Science, 2023, 19(1): 136-146. DOI: 10.12131/20220070
shu

Anesthetic effect and tissue oxidative injury for Litopenaeus vannamei by two anesthetics

  • 1.

    College of Fisheries and Life Science, Dalian Ocean University/Key Laboratory of Hydrobiology in Liaoning Province, Dalian 116023, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 3.

    Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

  • 4.

    Guangdong Nanwan Aquaculture Co., Ltd., Yangjiang 529500, China

  • 5.

    Yangjiang Fisheries Technical Extension Station, Yangjiang 529500, China

  • 6.

    Guangdong Shunxin Sea Fishery Group Co., Ltd., Yangjiang 529800, China

More Information
  • Received Date: March 18, 2022
  • Revised Date: July 17, 2022
  • Accepted Date: August 10, 2022
  • Available Online: August 14, 2022
    Graphical Abstract
    • Abstract
  • To provide references for the application of two anesthetics in Litopenaeus vannamei, we studied the anesthetic effects of eugenol (20, 30, 40, 60, 80, 100, 120, 140, 160 mg·L−1) and MS-222 (800, 900, 1 000, 1 100, 1 200, 1 300, 1 400, 1 500, 1 600 mg·L−1) on L. vannamei with body mass of (14±3) g. Besides, we tested the secondary stress injury on the physiological functions and pathomorphologic changes by the two anesthetics. The results show that: 1) Eugenol of 80 mg·L−1and MS-222 of 1 400 mg·L−1 induced L. vannamei to enter the stage of deep anesthetic within 3 min, and return to the normal stage within 5 min. 2) The gills and hepatopancreas of L. vannamei were injured by the two anesthetics, and the antioxidant indexes of gills in the eugenol group reached the maximum value earlier than the MS-222 group. The Caspase-3 activity in the eugenol group decreased significantly after anesthesia recovery of 6 h, while that in the MS-222 group increased significantly (P<0.05). The Na+/K+-ATPase activity in both the groups decreased significantly (P<0.05). Pathomorphologic analysis shows that the two anesthetics could cause deformation or lysis of gills and hepatopancreas, and the eugenol group could recover after anesthesia recovery of 6 h. In conclusion, eugenol and MS-222 have anesthetic effects on L. vannamei, but the concentration of MS-222 is higher and the secondary stress damage to the body is more serious.
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  • [1]
    农业农村部渔业渔政管理局, 全国水产技术推广总站, 中国水产学会. 2021中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2021: 21-36.
    [2]
    徐子涵, 茅林春. 虾保活运输的关键技术及装备研究进展[J]. 食品工业科技, 2018, 39(9): 306-310.
    [3]
    米红波, 侯晓荣, 茅林春. 鱼虾类保活运输的研究与应用进展[J]. 食品科学, 2013, 34(13): 365-369. doi: 10.7506/spkx1002-6630-201313076
    [4]
    MARKING L L, MEYER F P. Are better anesthetics needed in fisheries?[J]. Fisheries, 1985, 10(6): 2-5. doi: 10.1577/1548-8446(1985)010<0002:ABANIF>2.0.CO;2
    [5]
    Food and Drug Administration. Guidance for industry 150: status of clove oil and eugenol for anesthesia of fish [EB/OL]. (2007-4-24). https://www.fda.gov/media/69954/download.
    [6]
    杨洁, 朱晓玲. 丁香酚在水产品中的残留及风险评估研究进展[J]. 食品安全质量检测学报, 2020, 11(18): 6523-6529.
    [7]
    HIKASA Y, TAKASE K, OGASAWARA T, et al. Anesthesia and recovery with tricaine methanesulfonate, eugenol and thiopental sodium in the carp, Cyprinus carpio[J]. Jpn J Vet Sci, 1986, 48(2): 341-351. doi: 10.1292/jvms1939.48.341
    [8]
    王文豪, 董宏标, 孙永旭, 等. MS-222 和丁香酚在大口黑鲈幼鱼模拟运输中的麻醉效果[J]. 南方水产科学, 2018, 14(6): 52-58. doi: 10.12131/20180100
    [9]
    杜浩, 危起伟, 杨德国, 等. MS-222、丁香油、苯唑卡因对养殖美洲鲥幼鱼的麻醉效果[J]. 大连海洋大学学报, 2016, 22(1): 20-26.
    [10]
    郝长杰, 庄平, 赵峰, 等. MS 222 和丁香酚对暗纹东方鲀幼鱼麻醉效果的比较研究[J]. 海洋渔业, 2019, 41(5): 530-538.
    [11]
    张丽, 汪之和. MS-222 对大黄鱼成鱼麻醉效果的研究[J]. 湖南农业科学, 2010, 18(14): 38-40. doi: 10.3969/j.issn.1006-060X.2010.14.014
    [12]
    COYLE S D, DASGUPTA S, TIDWELL J H, et al. Comparative efficacy of anesthetics for the freshwater prawn Macrobrachiurn rosenbergii[J]. J World Aquacult Soc, 2005, 36(3): 282-290.
    [13]
    徐德峰, 吴嘉鑫, 孙力军, 等. 丁香酚和MS-222对凡纳滨对虾麻醉效果比较[J]. 广东海洋大学学报, 2021, 41(5): 44-52. doi: 10.3969/j.issn.1673-9159.2021.05.006
    [14]
    蔡诤, 董宏标, 王军, 等. 浓度和温度对丁香酚麻醉日本囊对虾效果的影响[J]. 海洋科学, 2012, 36(3): 29-36.
    [15]
    徐滨, 魏开金, 朱祥云, 等. MS-222 和丁香酚对克氏原螯虾幼虾耗氧率, 排氨率及窒息点的影响[J]. 大连海洋大学学报, 2018, 33(4): 455-459.
    [16]
    彭勤, 黄和, 赵子科. 丁香酚麻醉剂在凡纳滨对虾中残留消除的研究[J]. 广东海洋大学学报, 2018, 38(5): 64-70. doi: 10.3969/j.issn.1673-9159.2018.05.010
    [17]
    BARBAS L A L, PEREIRA-CARDONA P M, MALTEZ L C, et al. Anaesthesia and transport of juvenile tambaqui Colossoma macropomum (Cuvier, 1818) with tricaine methane-sulphonate: implications on secondary and oxidative stress responses[J]. J Appl Ichthyol, 2017, 33(4): 720-730. doi: 10.1111/jai.13382
    [18]
    平洪领, 史会来, 余方平, 等. MS-222 对日本对虾麻醉效果的研究[J]. 水产养殖, 2018, 39(4): 1-4. doi: 10.3969/j.issn.1004-2091.2018.04.001
    [19]
    TSUCHIYA H. Anesthetic agents of plant origin: a review of phytochemicals with anesthetic activity[J]. Molecules, 2017, 22(8): 1369-1403. doi: 10.3390/molecules22081369
    [20]
    SINGH A K, KUMAR S, VINAYAK M. Recent development in antihyperalgesic effect of phytochemicals: anti-inflammatory and neuro-modulatory actions[J]. Inflamm Res, 2018, 67(8): 633-654. doi: 10.1007/s00011-018-1156-5
    [21]
    BUTTERWORTH J F, STRICHARTZ G R. Molecular mechanisms of local anesthesia: a review[J]. Anesthesiology, 1990, 72(4): 711-734. doi: 10.1097/00000542-199004000-00022
    [22]
    JAVAHERY S, NEKOUBIN H, MORADLU A H. Effect of anaesthesia with clove oil in fish[J]. Fish Physiol Biochem, 2012, 38(6): 1545-1552. doi: 10.1007/s10695-012-9682-5
    [23]
    孙盛明, 祝孟茹, 潘方艳, 等. 低氧对甲壳动物的影响及其分子调控研究进展[J]. 水产学报, 2020, 44(4): 690-704.
    [24]
    何新龙, 傅洪拓, 龚永生, 等. 丁香酚对日本沼虾麻醉效果的研究[J]. 中国农学通报, 2007, 23(9): 620-623. doi: 10.3969/j.issn.1000-6850.2007.09.132
    [25]
    KOHEN R, NYSKA A. Invited review: Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification[J]. Toxicol Pathol, 2002, 30(6): 620-650. doi: 10.1080/01926230290166724
    [26]
    LEI Y, SUN Y, WANG X, et al. Effect of dietary phosphorus on growth performance, body composition, antioxidant activities and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis)[J]. Aquaculture, 2021, 531: 735856. doi: 10.1016/j.aquaculture.2020.735856
    [27]
    WANG W, DONG H, SUN Y, et al. Immune and physiological responses of juvenile Chinese sea bass (Lateolabrax maculatus) to eugenol and tricaine methanesulfonate (MS-222) in gills[J]. Aquac Rep, 2020, 18: 100554. doi: 10.1016/j.aqrep.2020.100554
    [28]
    刘晓华. 谷胱甘肽对凡纳滨对虾抗氧化防御的调控机理[D]. 武汉: 华中农业大学, 2010: 41-48.
    [29]
    熊大林, 段亚飞, 徐敬明, 等. 凡纳滨对虾鳃组织对高温和氨氮胁迫的生理响应[J]. 南方农业学报, 2020, 51(9): 2296-2303. doi: 10.3969/j.issn.2095-1191.2020.09.031
    [30]
    相启森, 孟旭, 乔燕, 等. 鼠尾草酸对自由基诱导蛋白质氧化损伤的保护作用[J]. 食品科学, 2013, 34(15): 281-284. doi: 10.7506/spkx1002-6630-201315058
    [31]
    RAJAKUMAR D V, RAO M N A. Dehydrozingerone and isoeugenol as inhibitors of lipid peroxidation and as free radical scavengers[J]. Biochem Pharmacol, 1993, 46(11): 2067-2072. doi: 10.1016/0006-2952(93)90649-H
    [32]
    SCATENA R, BOTTONI P, BOTTA G, et al. The role of mitochon dria in pharmacotoxicology: a reevaluation of an old, newly emerging topic[J]. Am J Physiol Cell Physiol, 2007, 293(1): 12-21. doi: 10.1152/ajpcell.00314.2006
    [33]
    吴任, 谢数涛, 孙勇, 等. 凡纳滨对虾热休克蛋白70的原核高效表达[J]. 中国水产科学, 2006, 13(2): 305-309. doi: 10.3321/j.issn:1005-8737.2006.02.022
    [34]
    贾旭颖, 张丹, 路允良, 等. 温度突变和非离子氨胁迫对淡水养殖凡纳滨对虾cyt-C和caspase-3的影响[J]. 中国水产科学, 2014, 21(4): 700-710.
    [35]
    DUAN Y, WANG Y, DONG H, et al. Physiological and immune response in the gills of Litopenaeus vannamei exposed to acute sulfide stress[J]. Fish Shellfish Immunol, 2018, 81: 161-167. doi: 10.1016/j.fsi.2018.07.018
    [36]
    王文豪. 2种麻醉剂对中国花鲈麻醉效应、损伤机理及其防护研究[D]. 上海: 上海海洋大学, 2020: 40-44.
    [37]
    POUNDER K C, MITCHELL J L, THOMSON J S, et al. Physiological and behavioural evaluation of common anaesthesia practices in the rainbow trout[J]. Appl Anim Behav Sci, 2018, 199: 94-102. doi: 10.1016/j.applanim.2017.10.014
    [38]
    SOIVIO A, NYHOLM K, HUHTI M. Effects of anaesthesia with MS-222, neutralized MS-222 and benzocaine on the blood constituents of rainbow trout, Salmo gairdneri[J]. J Fish Biol, 1977, 10(1): 91-101. doi: 10.1111/j.1095-8649.1977.tb04045.x
    [39]
    苏家齐, 祝华萍, 朱长波, 等. 盐度和钠离子/钾离子对凡纳滨对虾幼虾存活与组织结构的影响[J]. 南方水产科学, 2021, 17(5): 45-53. doi: 10.12131/20210011
    [40]
    陶易凡, 强俊, 王辉, 等. 低pH胁迫对克氏原螯虾鳃和肝胰腺酶活力及组织结构的影响[J]. 中国水产科学, 2016, 23(6): 1279-1289.
    [41]
    洪美玲, 陈立侨, 顾顺樟, 等. 氨氮胁迫对中华绒螯蟹免疫指标及肝胰腺组织结构的影响[J]. 中国水产科学, 2007, 14(3): 412-418. doi: 10.3321/j.issn:1005-8737.2007.03.010
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