Volume 16 Issue 4
Aug.  2020
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ZHUANG Mingge, JIANG Shigui, ZHOU Falin, HUANG Jianhua, YANG Qibin, JIANG Song, YANG Lishi. Molecular cloning and multifunction exploration of CFSH gene in Penaeus monodon[J]. South China Fisheries Science, 2020, 16(4): 90-99. DOI: 10.12131/20200011
Citation: ZHUANG Mingge, JIANG Shigui, ZHOU Falin, HUANG Jianhua, YANG Qibin, JIANG Song, YANG Lishi. Molecular cloning and multifunction exploration of CFSH gene in Penaeus monodon[J]. South China Fisheries Science, 2020, 16(4): 90-99. DOI: 10.12131/20200011
shu

Molecular cloning and multifunction exploration of CFSH gene in Penaeus monodon

  • 1.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

  • 2. Tropical Fishery Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China

  • 3.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 4.

    Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

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  • Received Date: January 27, 2020
  • Revised Date: March 27, 2020
  • Accepted Date: April 09, 2020
  • Available Online: April 27, 2020
    Graphical Abstract
    • Abstract

  • We cloned the ORF and 3'UTR of PmCFSH [Crustacean female sex hormone (CFSH) gene of Penaeus monodon] by RACE (Rapid-amplification of cDNA ends) method. It encodes a protein of 214 aa which contains a signal peptide and an interleukin 17E (IL-17E) domain relating to immune response. The qRT-PCR results showed that PmCFSH was expressed in various tissues, among which the highest expression level was found in the abdominal nerve tissue. The expression level gradually increased from fertilized egg stage to larval stage, and reached the maximum at larval stage. During the ovarian development Stage II to IV, it gradually increased as the ovary matured. Both Gram-negative and positive bacteria could increase the PmCFSH expression which was maximum in gill at 3rd hour after Vibrio harveyi infection and 12th hour after Staphylococcus aureus stimulation, while that in hepatopancreas reached the maximum at 3rd hour after V. harveyi infection and 48th hour after S. aureus stimulation. The study reveals that PmCFSH gene not only participates in the regulation of larval development and gonad maturity, but also has an immune response to bacterium, reflecting the "multifunction" of hormones.

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  • [1]
    许成团, 方良智. 非洲斑节对虾健康养殖技术[J]. 海洋与渔业, 2016(6): 58-60.
    [2]
    OKUMURA T. Perspectives on hormonal manipulation of shrimp reproduction[J]. Jpn Agr Res Q, 2004, 38(1): 49-54. doi: 10.6090/jarq.38.49
    [3]
    江世贵. 斑节对虾种虾繁育技术[M]. 北京: 海洋出版社, 2013: 21-32.
    [4]
    ZMORA N, CHUNG J S. A novel hormone is required for the development of reproductive phenotypes in adult female crabs[J]. Endocrinology, 2014, 155(1): 230-239. doi: 10.1210/en.2013-1603
    [5]
    VENTURA T, CUMMINS S F, FITZGIBBON Q, et al. Analysis of the central nervous system transcriptome of the eastern rock lobster Sagmariasus verreauxi reveals its putative neuropeptidome[J]. PLoS One, 2014, 9(5): e97323. doi: 10.1371/journal.pone.0097323
    [6]
    LIU A, LIU J, LIU F, et al. Crustacean female sex hormone from the mud crab Scylla paramamosain is highly expressed in prepubertal males and inhibits the development of androgenic gland[J]. Front Physiol, 2018, 924(9): 1-11.
    [7]
    VEENSTRA J A. The power of next-generation sequencing as illustrated by the neuropeptidome of the crayfish Procambarus clarkii[J]. Gen Comp Endocr, 2015, 224(1): 84-95.
    [8]
    THONGBUAKAEW T, SUWANSA-ARD S, SRETARUGSA P, et al. Identification and characterization of a crustacean female sex hormone in the giant freshwater prawn, Macrobrachium rosenbergii[J]. Aquaculture, 2019, 507: 56-68. doi: 10.1016/j.aquaculture.2019.04.002
    [9]
    KOTAKA S, OHIRA T. cDNA cloning and in situ localization of a crustacean female sex hormone-like molecule in the kuruma prawn Marsupenaeus japonicus[J]. Fish Sci, 2018, 84(1): 53-60. doi: 10.1007/s12562-017-1152-7
    [10]
    SIGURDARDOTTIR S, ZAPADKA T E, LINDSTROM S I, et al. Diabetes-mediated IL-17A enhances retinal inflammation, oxidative stress, and vascular permeability[J]. Cell Immunol, 2019, 341: 103921.
    [11]
    LI S H, LI F H, WANG B, et al. Cloning and expression profiles of two isoforms of a CHH-like gene specifically expressed in male Chinese shrimp, Fenneropenaeus chinensis[J]. Gen Comp Endocr, 2010, 167(2): 308-316. doi: 10.1016/j.ygcen.2010.03.028
    [12]
    LACOMBE C, GREVE P, MARTIN G. Overview on the sub-grouping of the crustacean hyperglycemic hormone family[J]. Neuropeptides, 1999, 33(1): 71-80. doi: 10.1054/npep.1999.0016
    [13]
    黄建华, 周发林, 马之明, 等. 南海北部斑节对虾卵巢发育的形态及组织学观察[J]. 热带海洋学报, 2006, 25(3): 47-52. doi: 10.3969/j.issn.1009-5470.2006.03.009
    [14]
    QIN Y, JIANG S, HUANG J, et al. C-type lectin response to bacterial infection and ammonia nitrogen stress in tiger shrimp (Penaeus monodon)[J]. Fish Shellfish Immunol, 2019, 90: 188-198.
    [15]
    丁阳阳, 江世贵, 李运东, 等. 斑节对虾Pellino基因的克隆及其在不同胁迫条件下的表达分析[J]. 南方水产科学, 2019, 15(3): 87-96. doi: 10.12131/20180216
    [16]
    BULAJ G. Formation of disulfide bonds in proteins and peptides[J]. Biotechnol Adv, 2005, 23(1): 87-92. doi: 10.1016/j.biotechadv.2004.09.002
    [17]
    PIERCE J G, PARSONS T F A. Glycoprotein hormones: structure and function[J]. Annu Rev Biochem, 1981, 50(1): 465-495. doi: 10.1146/annurev.bi.50.070181.002341
    [18]
    SKINNER D C, ALBERTSON A J, NAVRATIL A, et al. Effects of gonadotrophin-releasing hormone outside the hypothalamic-pituitary-reproductive axis[J]. J Neuroendocrinol, 2009, 21(4): 282-292. doi: 10.1111/j.1365-2826.2009.01842.x
    [19]
    XU S L, WANG D L, JIA C Y, et al. Effects of Vibrio alginolyticus infection on immune-related enzyme activities and ultrastructure of Charybdis japonica gills[J]. Aquaculture, 2013, 396/397/398/399(1): 82-88.
    [20]
    DU J, ZHU H, LIU P, et al. Immune responses and gene expression in hepatopancreas from Macrobrachium rosenbergii challenged by a novel pathogen spiroplasma MR-1008[J]. Fish Shellfish Immun, 2013, 34(1): 315-323. doi: 10.1016/j.fsi.2012.11.009
    [21]
    SHABGAH A G, FATTAHI E, SHAHNEH F Z. Interleukin-17 in human inflammatory diseases[J]. Adv Dermatol Allergol, 2014, 31(4): 256-261.
    [22]
    周光炎. 免疫学原理[M]. 3版. 北京: 科学出版社, 2013: 217-218, 281-286.
    [23]
    KUMAR P, MONIN L, CASTILLO P, et al. Intestinal interleukin-17 receptor signaling mediates reciprocal control of the gut microbiota and autoimmune inflammation[J]. Immunity, 2016, 44(3): 659-671. doi: 10.1016/j.immuni.2016.02.007
    [24]
    WU S Z, HUANG X D, LI Q, et al. Interleukin-17 in pearl oyster (Pinctada fucata): molecular cloning and functional characterization[J]. Fish Shellfish Immun, 2013, 34(5): 1050-1056. doi: 10.1016/j.fsi.2013.01.005
    [25]
    JEONG Y H, PARK J S, KIM D H, et al. Anti-inflammatory mechanism of lonchocarpine in LPS- or poly (I:C)-induced neuroinflammation[J]. Pharmacol Res, 2017, 119: 431-442. doi: 10.1016/j.phrs.2017.02.027
    [26]
    LI C, CHEN Y, WENG S, et al. Presence of tube isoforms in Litopenaeus vannamei suggests various regulatory patterns of signal transduction in invertebrate NF-κB pathway[J]. DCI, 2014, 42(2): 174-185.
    [27]
    张健, 张志峰, 邵明瑜. 中国明对虾脑发生和分化的细胞学观察[J]. 中国水产科学, 2007, 14(1): 15-22. doi: 10.3321/j.issn:1005-8737.2007.01.003
    [28]
    谢松, 李理想, 陈宏健, 等. 中国明对虾卵黄蛋白原基因启动子克隆与分析[J]. 河北大学学报(自然科学版), 2013, 33(2): 175-180.
    [29]
    韩萍, 杨丽诗, 吴松, 等. 促性腺激素释放激素及多巴胺拮抗物地欧酮对斑节对虾卵巢组织发育的影响[J]. 南方水产科学, 2015, 11(2): 50-56.
    [30]
    TSUTSUI N, KOTAKA S, OHIRA T, et al. Characterization of distinct ovarian isoform of crustacean female sex hormone in the kuruma prawn, Marsupenaeus japonicus[J]. Comp Biochem Physiol A, 2018, 217: 7-16. doi: 10.1016/j.cbpa.2017.12.009
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