| Citation: |
LI Ying, LIU Zhenxing, MA Yanping, CAO Junming, MA Jiangyao, HAO Le, LIANG Zhiling, KE Hao, LI Yugu. Protection of Jian carp with immunization of CyHV-3 ORF65 DNA vaccine[J]. South China Fisheries Science, 2019, 15(4): 122-126. DOI: 10.12131/20190033
|
In order to develop a DNA vaccine against Cyprinid herpes virus 3 (CyHV-3), also known as Koi herpesvirus (KHV), we inserted CyHV-3 ORF65 into pEGFP-N1 to construct recombinant plasmid pEGFP-ORF65. The expression of pORF65-EGFP fusion protein in Cyprinus carpio var. Jian was confirmed by transfection and in vivo imaging experiments. The recombinant plasmid was injected into the tail stalk of the carp seedlings at dosage of 3 μg·tail−1 for about 10 g and immunized for three times. ELISA detection shows that pEGFP-ORF65 could significantly increase the level of serum specific antibodies. The challenge test shows that 21 d after CyHV-3 challenge, the survival rates of PBS group, pEGFP-N1 group and pEGFP-ORF65 group were 25%, 30% and 90%, respectively, which significantly improved the survival rate of the carp seedlings (P<0.01). The study provides a basis for the application of ORF65 as a target gene of DNA vaccine in the prevention and control of CyHV-3.
| [1] |
DAVISON A J, EBERLE R, EHLERS B, et al. The order Herpesvirales[J]. Arch Virol, 2009, 154(1): 171-177. doi: 10.1007/s00705-008-0278-4
|
| [2] |
RAKUS K, OUYANG P, BOUTIER M, et al. Cyprinid herpesvirus 3: an interesting virus for applied and fundamental research[J]. Vet Res, 2013, 44(1): 85. doi: 10.1186/1297-9716-44-85
|
| [3] |
GARVER K A, AL-HUSSINEE L, HAWLEY L M, et al. Mass mortality associated with koi herpesvirus in wild common carp in Canada[J]. J Wild Dis, 2010, 46(4): 1242-1251. doi: 10.7589/0090-3558-46.4.1242
|
| [4] |
MINAMOTO T, HONJO M N, UCHII K, et al. Detection of cyprinid herpesvirus 3 DNA in river water during and after an outbreak[J]. Vet Microbiol, 2009, 135(3): 261-266.
|
| [5] |
TROSZOK A, KOLEK L, SZCZYGIEL J A, et al. Acyclovir inhibits Cyprinid herpesvirus 3 multiplication in vitro[J]. J Fish Dis, 2018, 41(11): 1709-1718. doi: 10.1111/jfd.2018.41.issue-11
|
| [6] |
GAO Y, SUAREZ N M, WILKIE G S, et al. Genomic and biologic comparisons of Cyprinid herpesvirus 3 strains[J]. Vet Res, 2018, 49(1): 40. doi: 10.1186/s13567-018-0532-z
|
| [7] |
YASUMOTO S, KUZUYA Y, YASUDA M A, et al. Oral immunization of common carp with a liposome vaccine fusing koi herpesvirus antigen[J]. Fish Pathol, 2006, 41(4): 141-145. doi: 10.3147/jsfp.41.141
|
| [8] |
PERELBERG A, RONEN A, HUTORAN M, et al. Protection of cultured Cyprinus carpio against a lethal viral disease by an attenuated virus vaccine[J]. Vaccine, 2005, 23(26): 3396-3403. doi: 10.1016/j.vaccine.2005.01.096
|
| [9] |
COSTES B, FOURNIER G, MICHEL B, et al. Cloning of the koi herpesvirus genome as an infectious bacterial artificial chromosome demonstrates that disruption of the thymidine kinase locus induces partial attenuation in Cyprinus carpio koi[J]. J Virol, 2008, 82(10): 4955-4964. doi: 10.1128/JVI.00211-08
|
| [10] |
SCHRODER L, KLAFACK S, BERGMANN S M, et al. Characterization of gene deletion mutants of Cyprinid herpesvirus 3 (koi herpesvirus) lacking the immunogenic envelope glycoproteins pORF25, pORF65, pORF148 and pORF149[J]. Virus Res, 2019, 261: 21-30. doi: 10.1016/j.virusres.2018.12.004
|
| [11] |
FRANCIS M J. Recent advances in vaccine technologies[J]. Vet Clin N Am, 2018, 48(2): 231-241. doi: 10.1016/j.cvsm.2017.10.002
|
| [12] |
杨海, 王芳宇. DNA疫苗的研究进展[J]. 中国畜牧兽医, 2013, 40(1): 72-76. doi: 10.3969/j.issn.1671-7236.2013.01.019
|
| [13] |
SHEDLOCK D J, WEINER D B. DNA vaccination: antigen presentation and the induction of immunity[J]. J Leukocyte Biol, 2000, 68(6): 793-806.
|
| [14] |
FAUREZ F, DORY D, le MOIGNE V, et al. Biosafety of DNA vaccines: new generation of DNA vectors and current knowledge on the fate of plasmids after injection[J]. Vaccine, 2010, 28(23): 3888-3895. doi: 10.1016/j.vaccine.2010.03.040
|
| [15] |
LIU M A. DNA vaccines: an historical perspective and view to the future[J]. Immunol Rev, 2011, 239(1): 62-84. doi: 10.1111/imr.2010.239.issue-1
|
| [16] |
COLLINS C, LORENZEN N, COLLET B. DNA vaccination for finfish aquaculture[J]. Fish Shellfish Immun, 2019, 85(SI): 106-125.
|
| [17] |
ZHOU J X, WANG H, LI X W, et al. Construction of KHV-CJ ORF25 DNA vaccine and immune challenge test[J]. J Fish Dis, 2014, 37(4): 319-325. doi: 10.1111/jfd.2014.37.issue-4
|
| [18] |
ZHOU J X, XUE J D, WANG Q J, et al. Vaccination of plasmid DNA encoding ORF81 gene of CJ strains of KHV provides protection to immunized carp[J]. In Vitro Cell Dev Biol Anim, 2014, 50(6): 489-495. doi: 10.1007/s11626-014-9737-2
|
| [19] |
EMBREGTS C W E, TADMOR-LEVI R, VESELY T, et al. Intra-muscular and oral vaccination using a koi herpesvirus ORF25 DNA vaccine does not confer protection in common carp (Cyprinus carpio L.)[J]. Fish Shellfish Immun, 2018, 85: 90-98.
|
| [20] |
谭爱萍, 邹为民, 赵飞, 等. 锦鲤疱疹病毒主要囊膜蛋白基因的PCR扩增与序列分析[J]. 中国水产科学, 2008, 15(5): 880-884. doi: 10.3321/j.issn:1005-8737.2008.05.023
|
| [21] |
FUCHS W, GRANZOW H, DAUBER M, et al. Identification of structural proteins of koi herpesvirus[J]. Arch Virol, 2014, 159(12): 3257-3268. doi: 10.1007/s00705-014-2190-4
|
| [22] |
YI Y, ZHANG H, LEE X, et al. Extracellular virion proteins of two Chinese CyHV-3/KHV isolates, and identification of two novel envelope proteins[J]. Virus Res, 2014, 191(10): 108-116.
|
| [23] |
ZHENG S, LI Y, WANG Q, et al. Identification of a novel envelope protein encoded by ORF136 from Cyprinid herpesvirus 3[J]. Arch Virol, 2017, 162(12): 3849-3853. doi: 10.1007/s00705-017-3528-5
|
| [24] |
刘振兴. 鲤疱疹病毒3型毒株的分离、分析及免疫学防治研究[D]. 广州: 华南农业大学, 2015: 72-89.
|
| [25] |
马艳平, 郝乐, 梁志凌, 等. 基于间接ELISA的鲤血清CyHV-3 pORF65抗体检测方法的建立[J]. 南方水产科学, 2018, 14(3): 113-119. doi: 10.3969/j.issn.2095-0780.2018.03.014
|
| [26] |
EL-DIN M M M. Histopathological studies in experimentally infected Koi carp (Cyprinus carpio koi) with koi herpesvirus in Japan[J]. World J Fish Mar Sci, 2011, 3(3): 252-259.
|
| [27] |
GRAY W L, MULLIS L, LAPATRA S E, et al. Detection of koi herpesvirus DNA in tissues of infected fish[J]. J Fish Dis, 2010, 25(3): 171-178.
|
| [28] |
PIKARSKY E, RONEN A, ABRAMOWITZ J, et al. Pathogenesis of acute viral disease induced in fish by carp interstitial nephritis and gill necrosis virus[J]. J Virol, 2004, 78(17): 9544-9551. doi: 10.1128/JVI.78.17.9544-9551.2004
|
| [29] |
SIWICKI A, KAZUŃ K, KAZUŃ B, et al. Impact of cyprinid herpesvirus-3, which causes interstitial nephritis and gill necrosis, on the activity of carp (Cyprinus carpio L.) macrophages and lymphocytes[J]. Arch Pol Fish, 2012, 20: 123-128.
|
| [30] |
KURATH G. Biotechnology and DNA vaccines for aquatic animals[J]. Rev Sci Tech, 2008, 27(1): 175-196. doi: 10.20506/rst.issue.27.1.37
|
| [31] |
DANIELA R, KLUPP B G, TEIFKE J P, et al. Identification of envelope protein pORF81 of koi herpesvirus[J]. J Gen Virol, 2008, 89(4): 896-900. doi: 10.1099/vir.0.83565-0
|
| [1] | WANG Shaomin, MA Zhenhua, HU Jing, WANG Wenfei, MAO Fukao, BAI Zemin, WANG Lixian. Hydrodynamic performance of a square-type submersible net cage under combined wind, wave and current conditions[J]. South China Fisheries Science, 2025, 21(2): 1-13. DOI: 10.12131/20240245 |
| [2] | FU Zhiguo, DING Guolin, DUAN Jinghui, YAO Yunpeng, WANG Lin. Calculation method of hydrodynamic load factors for aquaculture platform netting[J]. South China Fisheries Science. DOI: 10.12131/20240279 |
| [3] | JIANG Shuxia, KONG Xianghong, HUANG Xiaoshuang, YE Xuchang, CAO Daomei. Study on hydrodynamic characteristics and flow field visualization of multi-blade controllable otter board based on CFD[J]. South China Fisheries Science, 2024, 20(5): 136-148. DOI: 10.12131/20240095 |
| [4] | LIU Jingbin, TANG Hao, XU Liuxiong, SUN Qiuyang, LIU Wei, YIN Liqiang, ZHANG Feng. Evaluation of scale effect on hydrodynamic force of V-shaped otter board based on CFD[J]. South China Fisheries Science, 2022, 18(5): 128-137. DOI: 10.12131/20210355 |
| [5] | HAO Yuxin, WAN Rong, ZHOU Cheng, YE Xuchang, GUAN Qinglong, ZHANG Xiaoxian. Hydrodynamic performance of Argentine shortfin squid (Illex argentinus) bottom trawl[J]. South China Fisheries Science, 2022, 18(5): 118-127. DOI: 10.12131/20210343 |
| [6] | SUI Liuyang, HUANG Xiaohua, LIU Haiyang, HU Yu, YUAN Taiping, WANG Shaomin, TAO Qiyou. Effects of mooring pattern on dynamic characteristics of a deep-water aquaculture cage[J]. South China Fisheries Science, 2021, 17(4): 98-108. DOI: 10.12131/20210049 |
| [7] | WANG Shaomin, YUAN Taiping, YANG Xieqiu, TAO Qiyou, SHEN Wei, HU Yu, HUANG Xiaohua. Frequency domain analysis of hydrodynamic characteristics of mariculture ship with truss and plate frame hybrid structure[J]. South China Fisheries Science, 2021, 17(4): 82-90. DOI: 10.12131/20210021 |
| [8] | LI Jie, YAN Lei, YANG Bingzhong, ZHANG Peng. Numerical simulation on untrammeled settlement process of falling-net[J]. South China Fisheries Science, 2017, 13(4): 105-114. DOI: 10.3969/j.issn.2095-0780.2017.04.013 |
| [9] | LIU Jian, HUANG Hongliang, WU Yue, RAO Xin, LI Lingzhi, CHEN Shuai, YANG Jialiang, ZHOU Bin. Model test of hydrodynamic characteristics of two types of vertical cambered slotted otter boards[J]. South China Fisheries Science, 2015, 11(1): 68-74. DOI: 10.3969/j.issn.2095-0780.2015.01.010 |
| [10] | LIU Lili, WAN Rong, WANG Xijie, WANG Sijie, WANG Yunzhong. Analysis of effects of fishery stock enhancement based on system dynamics model[J]. South China Fisheries Science, 2012, 8(1): 16-23. DOI: 10.3969/j.issn.2095-0780.2012.01.003 |
| 1. |
李明. 七轴浇铸机器人的运动学建模和仿真技术. 现代制造技术与装备. 2024(02): 215-217 .
|
Supported by: Beijing Renhe Information Technology Co., Ltd.
粤公网安备 44010502001741号
DownLoad: