Volume 11 Issue 3
Jun.  2015
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WANG Xiaomei, CHEN Chengxun, XING Kezhi, WANG Yan, DAI Wei, XUE Suqing. Analysis of daily weight gain and GH mRNA expression in juvenile Clarias gariepinu reared at different stocking densities[J]. South China Fisheries Science, 2015, 11(3): 35-40. DOI: 10.3969/j.issn.2095-0780.2015.03.006
Citation: WANG Xiaomei, CHEN Chengxun, XING Kezhi, WANG Yan, DAI Wei, XUE Suqing. Analysis of daily weight gain and GH mRNA expression in juvenile Clarias gariepinu reared at different stocking densities[J]. South China Fisheries Science, 2015, 11(3): 35-40. DOI: 10.3969/j.issn.2095-0780.2015.03.006
shu

Analysis of daily weight gain and GH mRNA expression in juvenile Clarias gariepinu reared at different stocking densities

  • Tianjin Key Lab. of Aqua-Ecology & Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

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  • Received Date: September 17, 2014
  • Revised Date: November 10, 2014
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  • Juvenile Clarias gariepinus were reared at densities of 35 kg · m-3, 65 kg · m-3, 95 kg · m-3 and 125 kg · m-3 for 60 d. On 30th and 60th day, we measured the daily weight gain (DWG) and assayed the mRNA level of GH in pituitary by semi-quantitative RT-PCR. During 0~30 d breeding, the DWGs showed no significant difference between 35 kg · m-3 and 65 kg m-3 treatments as well as between 95 kg · m-3 and 125 kg m-3 treatments, but the DWGs were significantly higher at 35 kg · m-3 and 65 kg · m-3 treatments than those at 95 kg · m-3 and 125 kg · m-3 treatments. While on 30th day, significant difference was not found in the GH mRNA level of pituitary between any two stocking densities. During 30~60 d breeding, the DWGs of 35 kg · m-3 and 65 kg · m-3 treatments were significantly higher than that of 125 kg · m-3 treatment, there was no significant difference in DWG between the other two treatments. On 60th day, the expressions of GH gene were significantly higher in 35 kg · m-3 and 65 kg · m-3 treatments than those in 95 kg · m-3 and 125 kg · m-3 treatments. The results indicate that there was no close correlation between DWG and mRNA level of GH in pituitary of juvenile C.gariepinus.

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