Sequence analysis of mitochondrial cytochrome b gene of Carassius auratus var.and phylogenetic relationships of C. auratus var.and C. auratus

Mitochondrial cytochrome b DNAs of representative varieties of Carassius auratus var., viz. grass goldfish, red dragoneye goldfish, red-head wen goldfish, blisters-eye goldfish and white-head oval goldfish were sequenced. 1 141 bp of mitochondril cytochrome b gene sequence has been obtained from C. auratus var. and the content of T, C, A, G of the gene were 29.1%, 27.8%, 28.4%, 14.5%, respectively.The sequences were same in those varieties of C. auratus var. So the results showed that Cyt b gene was conservative and indicated grass goldfish, red dragoneye goldfish, red-head wen goldfish, blisters-eye goldfish, white-head oval goldfish may be the same species. According to the analysis, C. auratus var. is relatively closer to C. auratus. Sequence analysis of mtDNA Cyt b gene of C. auratus var. and C. auratus which has 97.9% homology suggests it may be reasonable in a species.