Sequence analysis of mitochondrial cytochrome b+ gene of Carassius auratus var. and phylogenetic relationships of C.auratus var. and C.auratus

Mitochondrial cytochrome b DNAs of representative varieties of Carassius auratus var.，viz grass goldfish，red dragoneye goldfish, red-head wen goldfish，blisters-eye goldfish and white-head oval goldfish were sequenced. 1 141 bp of mitochondril cytochrome b gene sequence has been obtained from C.auratus var. and the content of T, C, A, G of the gene were 29.1%, 27.8%，28.4%, 14.5%, respectively. The sequences were same in those varieties of C.auratus var. So the results showed that Cyt b gene was conservative and indicated grass goldfish，red dragoneye goldfish，red-head wen goldfish，blisters-eye goldfish，white-head oval goldfish may be the same species. According to the analysis，C. auratus var. is relatively closer to C.auratus. Sequence analysis of mtDNA Cyt b gene of C. auratus var. and C.auratus which has 97.9% homology suggests it may be reasonable in a species.