Based on the data of four seasons survey in the continental shelf of northern South China Sea from 2006 to 2007, the species composition and resources density of Cephalopoda were analyzed. The results showed that there were 28 species belonging to 8 genus, 5 families, 3 orders in the area. The predominant species were Loligo edulis, L.duvaucelii and L.chinensis. The catch rate of Cephalopoda gradually increased from east to west in the area, with the highest in transect A (11.95 kg · h^-1) and lowest in transect E (7.20 kg · h^-1).It also showed obvious seasonal variation in catch rate, higher in summer and autumn and lower in winter and spring, with average of 9.53 kg · h^-1.Cephalopoda distributed mainly in the depth of 60 m to 180 m with the most living in the depth of about 100 m. The resources density of Cephalopoda was 127.10 kg · km^-2.

Survey of macrobenthic fauna at the 2 sections in the rocky intertidal zones of Qingdao (the east of the second bathing beach section and the small Qingdao island section) was carried out monthly from August, 2004 to May, 2005. Seventy-four species of nine phyla were collected. The biomass of the two sections were 3 498.80 and 3 512.00 g · m^-2 (wet weight), respectively, and the density of the two sections were 2 620.44 and 1 552 ind · m^-2, respectively. Mytilus edulis dominated the biomass and density of the macrobenthic fauna on the two sections. The biomass and density of the mid intertidal zone were greater than those of the low zone. The average Shannon-Wiener indices and Pielou′s evenness index of the two sections in this study were not high, but the indices of the small Qingdao island section were higher than those of the east of the second bathing beach section.

This paper reported the expression of envelope protein VP28 of white spot syndrome virus (WSSV) in recombinant Escherichia coli BL21 by using lactose as inducer.The results indicated that lactose could induce foreign protein expression and enhance cell growth during the induction period.The study showed that expression of VP28 fusion protein was up to 97.36 mg · L^-1 with the lactose concentration of 8 g · L^-1 and the optimal fermentation condition of 12 hours.In order to reduce the cultivation cost, the whey was used as alternative to lactose as carbon source and inducer for VP28 expression.The results showed that expression of VP28 fusion protein was up to 86.24 mg · L^-1 with the whey concentration of 10 g · L^-1 and the optimal fermentation condition of 13 hours.

The binding capacity of the drug enrofloxacin with the plasma protein of Chinese mitten-handed crab, Eriocheir sinensis during feeding was investigated. The method of fixed equilibrium dialysis test was applied to the study on the binding of enrofloxacin with the crab blood plasma protein. Concentration of the plasma proteins in Chinese mitten-handed crab was 63.95±0.29 mg · mL^-1. Molecular weight of these proteins were mainly between 66.2~97.1 kDa. Protein binding level of enrofloxacin and crab plasma proteins was 47.39%~82.58%, showing that more than 50% of the drug could be bound to the proteins. Thus, it could be concluded that enrofloxacin can bind with plasma proteins tightly. So, in case of using enrofloxacin alone or with other medicine, its effective concentration and competition between drugs should be considered in Chinese mitten-handed crab culture.

The histological structure and types of mucous cells in the digestive tracts (i.e., tongue, esophagus, stomach and intestines) of Rhabdosargus sarba were observed and analyzed by using HE and AB-PAS (alcian blue and periodic acid schiff′s reation) conbined staining. Results of study in which slices were stained with HE and showed that R.sarba has a broad oropharyngeal cavity and different type of teeth. The stomach is divided to three parts which are cardiac stomach, stomach caeca and pyloric stomach. The cardiac stomach has the most gastric pit and gastric gland while stomach caeca has the thickest wall. There are abundant mucous membrane pleats in the whole intestine. R.sarba has 8 to 10 pyloric caecas which structure is similar to anterior intestine. The dividing lines of liver lobules are dim. There are plenty of tubular tissue in hepatopancreas. Pancreatic acini and pancreatic islets distribute around main veins, acinar cells being stained into navy blue are closely arranged. According to AB-PAS, 4 types of the mucous cells in the digestive tracts of R.sarba were observed: typeⅠwas red, typeⅡ was blue, type Ⅲ was purple reddish, and typeⅣ was blue purple. Only a small quantity of type Ⅱ and type Ⅲ mucous cells were found occasionally in stomach. Most of mucous cells in the other digestive tracts were of type Ⅲ and type Ⅳ. The density of the mucous cells in the intestine was related to the functions of the different segments in the digestive system.

The correlation between diet lipid levels and liver morphology and histology of juvenile tilapia (Oreochromis niloticus×O.aureus) were studied. The juvenile tilapia were fed five diets with graded lipid levels of 0%, 2%, 4%, 6%, 8%, respectively, for 70 days. The results showed that weight gain rate (WGR) and condition factor (CF) increased first and decreased later with the growing lipid content level. The feed conversion ratio (FCR) decreased first and increased later contrarily. The color and volume of liver and gallbladder of tested fish were different for different lipid content diets. Liver cells diameter increased continuously with the growing lipid content level. Liver cells diameter of group 4 and 5 were significantly larger than that of groups 1, 2 and 3. The symptom of fatty liver disease was appeared obviously in group 4 and 5. Liver cells shifted normal location. Fatty roundels increased in liver cells. And liver cells were translucent and like vacuole. Juvenile tilapia formed fatty liver disease easily if diet lipid content exceeds 6%. It suggested that the supplement of over 4% lipid in diets of juvenile tilapia would be harmful for fish liver.

Thirty-three full-sib families of the Japanese scallops Patinopecten yessoensis were established using artificial insemination (1♂ : 3♀) in May 2006. The phenotype characters (shell length, shell height, shell width, wet weight) of scallops were measured at 4, 6, 8, 10, 12 months old. The data was analyzed using Excel and SPSS 13.0 software. The results showed that significant differences were found between different characters in different families at different growth stages. Among contemporary families, Nos. 06S-1, 06S-24 and 06S-25 grew fast in shell height, and Nos. 06S-1, 06S-25, 06S-18 and 06S-30 fast in body weight. Compared to these families, Nos. 06S-2, 06S-12 and 06S-21 had an obvious disadvantage in shell height, and Nos. 06S-2, 06S-5 and 06S-32 disadvantage in body weight. The order of monthly increase of shell height was similar to that in body weight in 33 families. According to the comparison above, Nos. 06S-24, 06S-25, 06S-30, 06S-1 and 06S-18 showed better in growth performance and Nos. 06S-2 and 06S-5 poor in growth traits. Obviously, significant differences in growth traits meant rich genetic resources. This program has provided some animals for further selective breeding in the future.

Complete sequence of mitochondrial DNA cytochrome b (Cyt b) gene in high-finned grouper (Cromileptes altivelis) was analyzed with PCR-sequencing.The 1 141 bp of Cyt b gene in C.altivelis was obtained. Base composition of Cyt b gene in C.altivelis were 29.1%, 32.3%, 23.2%, 14.0% for T, C, A and G, respectively, with 52.3% and 46.3% for A+T and C+G, respectively, which were similar to that in other fishes. The corresponding amino acid sequence was deduced and the percentage of Leu was 16.03%, much higher than any other amino acid.

The nutrient content was analyzed in th skin of tilapia and the conditions of gelatin extraction from the skin of tilapia were studied in this paper through the orthogonal experiment and the main physicochemical properties and microorganisms were also tested. The results showed that the contents of protein and collagen in the skin were 33.14% and 27.8%, respectively. Collagen accounted for 83.9% of all protein.The optimal conditions for gelatin extraction were established by soaking in 2.5% (W/V) NaOH solution for 3.5 h and in 0.02% (V/V) HCl for 3 h and incubated at temperature 55℃. Extraction yield of fish gelatin was 26.5%, gel strength was 468 g and the content of protein was 82.43%. The total number of bacteria was 1.3×10⁵ CFU · g^-1 and coliform bacteria indicated negative.

The nutritive composition and the contents of amino acids in muscle of Epinephelus lanceol were analyzed with routine methods, and its nutritive value was evaluated as well. The results showed that the contents of crude protein, crude fat, moisture and crude ash of the fresh muscles were 19.5%, 7.69%, 70.5%, 1.01%, respectively. The composition of amino acids of muscle was found to contain 18 common amino acids, and the content of 8 essential amino acids was 43.43%. It indicated that E. lanceol is a kind of fish with high protein, abundant amino acids, good edibility value and nutritive value.

The characteristic of the isothermal moisture sorption of grilled Ulva pertusa was studied. In the experiment, moisture absorption curve from 5℃ to 40℃ were drawn, and then corresponding regression equation of each curve was obtained by curve fitting.Mathematical model of corresponding relation between dry-basis moisture content and water activity of grilled U.pertusa under the condition of 5℃ to 40℃ was established according to the curve regression equation. The results showed that the moisture sorption isotherm of grilled U.pertusa was inverse-S shape from 5℃ to 40℃. The curve regression equation obtained by computer has big correlation coefficient and small calculation error.

Octopus species has a high degree of adaptability to farming environment. With widely distribution, rapid growth, short breeding cycle and higher nutritional value, Octopus vulgaris has become one of the most important species of Cephalopode. This paper gives a general introduction to its role in octopus culture in marine fishery, its ecology, biological characteristics, breeding biology, aquaculture research status, and exploitation prospect.

The progress and status of research on persistent organochlorines (POs) in cetaceans since 1970s were reviewed in this paper. The review focused on the following five aspects: the analysis methods for residue levels of POs in cetaceans, the ways of POs getting into and eliminating from cetacean body, the residue levels of POs in cetaceans, the harmful effect of POs on cetaceans, and assessment of the pollution levels of POs in cetaceans. The authors point out that the effects of POs on marine ecosystem will continue for a long time, and the long-term effect of POs on marine mammals in the coastal waters should be further studied.