Volume 4 Issue 5
Oct.  2008
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GUO Rui, PAN Rongchang, ZHOU Yubing, HU Wenfeng. Lactose-induced expression of envelope protein VP28 gene of white spot syndrome virus (WSSV) in recombinant Escherichia coli BL21[J]. South China Fisheries Science, 2008, 4(5): 16-22.
Citation: GUO Rui, PAN Rongchang, ZHOU Yubing, HU Wenfeng. Lactose-induced expression of envelope protein VP28 gene of white spot syndrome virus (WSSV) in recombinant Escherichia coli BL21[J]. South China Fisheries Science, 2008, 4(5): 16-22.
shu

Lactose-induced expression of envelope protein VP28 gene of white spot syndrome virus (WSSV) in recombinant Escherichia coli BL21

  • 1.

    Agricultural & Animal Husbandry Sciences Academy, Hetao University, Inner Mongolia Bayannaoer 015000, China

  • 2.

    College of Food Science, South China Agriculture University, Guangzhou 510642, China

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  • Received Date: March 15, 2008
  • Revised Date: April 30, 2008
    Graphical Abstract
    • Abstract

  • This paper reported the expression of envelope protein VP28 of white spot syndrome virus (WSSV) in recombinant Escherichia coli BL21 by using lactose as inducer.The results indicated that lactose could induce foreign protein expression and enhance cell growth during the induction period.The study showed that expression of VP28 fusion protein was up to 97.36 mg · L-1 with the lactose concentration of 8 g · L-1 and the optimal fermentation condition of 12 hours.In order to reduce the cultivation cost, the whey was used as alternative to lactose as carbon source and inducer for VP28 expression.The results showed that expression of VP28 fusion protein was up to 86.24 mg · L-1 with the whey concentration of 10 g · L-1 and the optimal fermentation condition of 13 hours.

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    • References (17)
  • [1]
    van HULTEN M C W, HULTEN V, WITTEVELDT J, et al. White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp[J]. Virol, 2001, 285(10): 228-233. doi: 10.1006/viro.2001.0928
    [2]
    许雅香, 许梓荣, 杜华华, 等. 对虾白斑综合征病毒VP19基因的克隆和序列分析[J]. 科技通报, 2003, 19(6): 473-476. https://www.doc88.com/p-5764546139278.html
    [3]
    ROBALINO J, BROWDY C L, PRIOR S, et al. Induction of antiviral immunity by double-stranded RNA in a marine invertebtate[J]. Virol, 2004, 321(10): 442- 448. doi: 10.1128/JVI.79.21.13561-13571.2005
    [4]
    NAMIKOSHI A, WU J L, YAMASHITA T, et al. Vaccination trails with Penaeus japonicus to induce resistance to white spot syndrome virus[J]. Aquac, 2004, 229(2): 25-35. doi: 10.1016/S0044-8486(03)00363-6
    [5]
    DONOVAN R S, ROBINSON C W, GLICK B R. Review: optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter[J]. Microbiol, 1996, 16(4): 145-154. doi: 10.1007/BF01569997
    [6]
    马新锋, 胡勤学, 崔天盆, 等. 可溶性DC-SIGN的表达、纯化及其生物学活性分析[J]. 中华微生物学和免疫学杂志, 2004, 26(1): 67-71. doi: 10.3760/j:issn:0254-5101.2004.01.021
    [7]
    余永恒, 李清雄, 王革非, 等. 乳糖诱导重组人睫状神经营养因子在大肠杆菌中的可溶性表达[J]. 中国生物制品学杂志, 2007, 20(1): 43-46. doi: 10.3969/j.issn.1004-5503.2007.01.012
    [8]
    刘俊红, 刘顺谊, 殷志敏. 乳糖诱导大肠杆菌中重组谷氨酰胺合成酶的表达[J]. 南京师大学报: 自然科学版, 2006, 29(3): 66-70. doi: 10.3969/j.issn.1001-4616.2006.03.015
    [9]
    段明瑞. 重组类人胶原蛋白基因工程菌发酵及动力学研究[D]. 西安: 西北大学, 2002. 10.3969/j.issn.1001-7631.2002.03.008
    [10]
    NEUBAUER P, LIN H Y, MATHISZIK B. Metabolic load of recombinant protein production: inhibition of cellular capacities for glucose uptake and respiration after induction of a heterologous gene in Escherichia coli[J]. Biotechnol, 2003, 83(2): 53-64. doi: 10.1002/bit.10645
    [11]
    王延华, 李官成, ZHOU Xinfu. 抗体理论与技术[M]. 北京: 科学出版社, 2005: 171-172. https://xueshu.baidu.com/usercenter/paper/show?paperid=2a07cb3e908068b806e79da5aeb9dfb9&site=xueshu_se
    [12]
    李兆鹏, 张栩, 徐斌, 等. 重组大肠杆菌高密度发酵中乳糖诱导表达hBLyS[J]. 过程工程学报, 2005, 5(4): 446-449.
    [13]
    李环, 陈悦, 朱孝霖, 等. 乳糖对产木聚糖酶基因工程菌1020的诱导作用及碳氮源优化[J]. 氨基酸和生物资源, 2005, 27(3): 22-26. doi: 10.3969/j.issn.1006-8376.2005.03.007
    [14]
    张毅, 屈贤铭, 杨胜利. 乳糖作为诱导剂对重组目的蛋白表达的影响[J]. 生物工程学报, 2000, 16(4): 464-468. doi: 10.3321/j.issn:1000-3061.2000.04.011
    [15]
    党红艳. 重组大肠杆菌生产肿瘤血管生长抑制因子kringle5发酵条件的优化及纯化[D]. 西安: 西北大学, 2005. https://www.zhangqiaokeyan.com/academic-degree-domestic_mphd_thesis/020314013157.html
    [16]
    储炬, 李友荣. 现代工业发酵调控学[M]. 北京: 化学工业出版社, 2002: 286-287. https://xueshu.baidu.com/usercenter/paper/show?paperid=ac80cf7c4df4b0c08b299165e0f23b93&site=xueshu_se&hitarticle=1
    [17]
    韩光烈. 谈谈扩大乳清粉利用范围及其现实意义[J]. 中国乳品工业, 1995, 23(1): 43-47.
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