Volume 4 Issue 5
Oct.  2008
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HUANG Hongbing, WU Guanghong, ZHANG Jingbo, CHEN Yi. Study on binding capacity of Enrofloxacin (ENR) with blood plasma protein of Chinese mitten-handed crab, Eriocheir sinensis[J]. South China Fisheries Science, 2008, 4(5): 23-27.
Citation: HUANG Hongbing, WU Guanghong, ZHANG Jingbo, CHEN Yi. Study on binding capacity of Enrofloxacin (ENR) with blood plasma protein of Chinese mitten-handed crab, Eriocheir sinensis[J]. South China Fisheries Science, 2008, 4(5): 23-27.
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Study on binding capacity of Enrofloxacin (ENR) with blood plasma protein of Chinese mitten-handed crab, Eriocheir sinensis

  • 1.

    Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, China

  • 2.

    Key Lab. of Agriculture and Animal Products Processing and Quality Control, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China

  • 3.

    Yancheng Institute of Supervision & Testing on Product Quality, Yancheng 224005, China

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  • Received Date: February 21, 2008
  • Revised Date: July 28, 2008
    Graphical Abstract
    • Abstract

  • The binding capacity of the drug enrofloxacin with the plasma protein of Chinese mitten-handed crab, Eriocheir sinensis during feeding was investigated. The method of fixed equilibrium dialysis test was applied to the study on the binding of enrofloxacin with the crab blood plasma protein. Concentration of the plasma proteins in Chinese mitten-handed crab was 63.95±0.29 mg · mL-1. Molecular weight of these proteins were mainly between 66.2~97.1 kDa. Protein binding level of enrofloxacin and crab plasma proteins was 47.39%~82.58%, showing that more than 50% of the drug could be bound to the proteins. Thus, it could be concluded that enrofloxacin can bind with plasma proteins tightly. So, in case of using enrofloxacin alone or with other medicine, its effective concentration and competition between drugs should be considered in Chinese mitten-handed crab culture.

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    • References (22)
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