Sequence analysis of mitochondrial cytochrome b gene of high-finned grouper (Cromileptes altivelis)

Complete sequence of mitochondrial DNA cytochrome b (Cyt b) gene in high-finned grouper (Cromileptes altivelis) was analyzed with PCR-sequencing.The 1 141 bp of Cyt b gene in C.altivelis was obtained. Base composition of Cyt b gene in C.altivelis were 29.1%, 32.3%, 23.2%, 14.0% for T, C, A and G, respectively, with 52.3% and 46.3% for A+T and C+G, respectively, which were similar to that in other fishes. The corresponding amino acid sequence was deduced and the percentage of Leu was 16.03%, much higher than any other amino acid.