Volume 16 Issue 6
Dec.  2020
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HU Xiaojuan, WEN Guoliang, TIAN Yajie, SU Haochang, XU Wujie, XU Yu, XU Yunna, CAO Yucheng. Removal effect of strain NB5 on ammonia nitrogen under different aquaculture conditions[J]. South China Fisheries Science, 2020, 16(6): 89-96. DOI: 10.12131/20200061
Citation: HU Xiaojuan, WEN Guoliang, TIAN Yajie, SU Haochang, XU Wujie, XU Yu, XU Yunna, CAO Yucheng. Removal effect of strain NB5 on ammonia nitrogen under different aquaculture conditions[J]. South China Fisheries Science, 2020, 16(6): 89-96. DOI: 10.12131/20200061
shu

Removal effect of strain NB5 on ammonia nitrogen under different aquaculture conditions

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs/Guangdong Provincal Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China

  • 2.

    Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

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  • Received Date: March 31, 2020
  • Revised Date: June 29, 2020
  • Accepted Date: July 08, 2020
  • Available Online: September 28, 2020
    Graphical Abstract
    • Abstract

  • We studied the growth of strain NB5 isolated from nitrifying bacteria and its removal effect on ammonia nitrogen (NH4 +-N) and nitrite nitrogen (NO2 −-N) under different aquaculture conditions, and identified this bacterium. The results show that strain NB5 grew well at salinity of 25−45, pH of 6.0−9.0, temperature of 15−35 ℃ and ventilations of 1−2 L·min−1 (P>0.05). At salinities of 45, 35 and 25, the maximum removal rates of NH4 +-N were 96.24%, 88.93% and 75.08%, respectively. At pHs of 7.5 and 9.0, they were 99.53% and 99.37%, respectively. At temperatures of 30, 25, 15 and 35 ℃, they were 99.53%, 97.22%, 97.29% and 71.26%, respectively. At ventilations of 2 and 1 L·min−1, they were 99.87% and 99.82%, respectively. Under the above conditions, the maximum removal rates of NH4 +-N were significantly higher than those of the control group and the other groups (P<0.05). Strain NB5 had no significant effect on NO2 −-N concentration under different aquaculture conditions (P>0.05). By 16S rDNA sequence analysis, strain NB5 was identified as Nitratireductor aquimarinus. The results indicate that strain NB5 has good environmental adaptability, especially suitable for aquaculture pond environment at medium and high salinities (25−45).

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