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REN Jie, LIN Weitie, LUO Xiaochun, XIE Mingquan. Statistical optimization of fermentation media for nitrite oxidizing bacteria[J]. South China Fisheries Science, 2008, 4(3): 7-14.
Citation: REN Jie, LIN Weitie, LUO Xiaochun, XIE Mingquan. Statistical optimization of fermentation media for nitrite oxidizing bacteria[J]. South China Fisheries Science, 2008, 4(3): 7-14.
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Statistical optimization of fermentation media for nitrite oxidizing bacteria

  • College of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, China

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  • Author Bio:

    REN Jie (1977-), male, Ph.D.candidate, mainly researches on industrial microbe and enviromental microbe. E-mail: renjiescut2001@163.com

  • Corresponding author:

    LIN Weitie, E-mail: 13902202933@139.com

  • Received Date: February 27, 2008
  • Revised Date: March 13, 2008
    Graphical Abstract
    • Abstract

  • The statistical experimental design (Plackett-Burman and Box-Behnken design) was applied to optimize the culture medium of nitrite oxidizing bacteria for improving the nitrite oxidizing rate. Estimated optimum medium composition of the nitrite oxidizing rate was as follows: NaHCO3 2.0 g · L-1; NaNO2 2.36 g · L-1; Na2CO3 0.37 g · L-1; NaCl 0.34 g ·L-1; KH2PO4 0.05 g · L-1; MgSO4 · 7H2O 0.05 g · L-1; and FeSO4 · 7H2O 0.03 g · L-1. The nitrite oxidizing rate reached a maximum at 905.0 mg NO2-N · (g MLSS · d)-1(mixed liquor suspended solids, MLSS).In the field trial, 50 L of nitrite oxidizing bacteria concentrate (1.99 g VSS · L-1)(volatile solid, VSS) with 850 mg NO2-N · (g MLSS · d)-1 were added to 0.6 hectares of the aquaculture water. Nitrite level in all treated ponds remained very low compared to the steady increase observed in all of the control ponds during 7 days.

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    • References (13)
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