Volume 18 Issue 2
Apr.  2022
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SHI Xiaoyi, DING Xiaoting, WAN Zixuan, YING Yu, LI Fuli, GAO Xin, FAN Yong. Mixotrophic and carbon fixation culture of nervonic acid-producing microalgae Mychonastes afer[J]. South China Fisheries Science, 2022, 18(2): 134-141. DOI: 10.12131/20210307
Citation: SHI Xiaoyi, DING Xiaoting, WAN Zixuan, YING Yu, LI Fuli, GAO Xin, FAN Yong. Mixotrophic and carbon fixation culture of nervonic acid-producing microalgae Mychonastes afer[J]. South China Fisheries Science, 2022, 18(2): 134-141. DOI: 10.12131/20210307
shu

Mixotrophic and carbon fixation culture of nervonic acid-producing microalgae Mychonastes afer

  • 1.

    College of Food Science and Technology, Ocean University of China, Qingdao 266071, China

  • 2.

    Qingdao Institute of Bioenergy and Bioprocss Technology, Chinese Academy of Science, Qingdao 266101, China

  • 3.

    Qingdao Institute for Food and Drug Control, Qingdao 266071, China

More Information
  • Received Date: October 21, 2021
  • Revised Date: December 13, 2021
  • Accepted Date: January 07, 2022
  • Available Online: January 26, 2022
    Graphical Abstract
    • Abstract
  • Microalgae are a group of single cell microorganism, which play an important role in aquatic production such as aquatic feeding and water regulating. This research focuses on the growth and photosynthetic capacity of Mychonastes afer and Chlorella sorokiniana under different nutrition and aeration conditions. The microalgae were cultivated under autotrophic, mixotrophic or heterotrophic conditions, respectively. And the influence of CO2 concentration was also investigated. The growth curve, photosynthetic electron transfer rate, organic carbon source utilization, photosynthetic oxygen evolution and respiratory oxygen consumption rate were measured to inflect the differences between M. afer and C. sorokiniana, and the emphasis was given to lipid components of M. afer. The results show that C. sorokiniana could grow under heterotrophic condition without light, while M. afer could not. The photosynthetic system of the two species were both inhibited by organic carbon sources, displaying lower efficiency of photosynthetic electron transfer rates and slower photosynthetic oxygen evolution rates. Besides, it is found that under mixotrophy condition, high concentration of CO2 was beneficial to the growth of M. afer, which promoted cell utilization of glucose, and increased the production of lipid and nervonic acid. The study explores a mixotrophic condition for M. afer to produce natural nervonic acid, shows significant differences between the two algae species in photosynthetic system, and explores the cooperative utilization of external organic carbon sources.
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    • References (35)
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