Citation: | WANG Zhilong, XIA Yun, XIE Jun, SHU Rui, WANG Guangjun, YU Ermeng, LEI Xiaoting, GONG Wangbao. Comparative analysis of muscle quality, intestinal morphology and microbial composition in two cultured frogs species[J]. South China Fisheries Science, 2024, 20(1): 173-183. DOI: 10.12131/20230159 |
In order to investigate the muscle quality, intestinal morphology and microbial composition of Hoplobatrachus tigerinus and Lithobates catesbeiana, we monitored the muscle nutritional components of two cultured frogs species by national food safety standard measurement method, and evaluated their muscle quality in addition with analysis of muscle texture and microstructure. Besides, we applied high-throughput sequencing and histological section technology to analyze the microbial community composition and microstructure of their gut. The results show that there was no significant difference in the total amino acids between H. tigerinus and L. catesbeiana (P>0.05). The unsaturated fatty acids content of L. catesbeiana were significantly higher than those of H. tigerinus (P<0.05). In addition, the muscle hardness, chewiness and gumminess of L. catesbeiana were significantly higher than those of H. tigerinus (P<0.05). The analyses of intestinal microbial communities show that the Shannon, Ace and Chao1 indexes of H. tigerinus were significantly higher than those of L. catesbeiana (P<0.05). At phylum level, the abundance of Firmicutes in L. catesbeiana was significantly higher than that in H. tigerinus (P<0.05), while the abundance of Actinobacteriota was significantly lower (P<0.05); At genus level, the abundance of Lactococcus and Escherichia-Shigella in H. tigerinus was significantly higher than that in L. catesbeiana (P<0.05), while Lactobacillus and Leuconostoc were significantly lower (P<0.05). The results show that there are differences in muscle quality between H. tigerinus and L. catesbeiana, but both have high nutritional value; high-throughput sequencing analyses show that there are differences in the composition of intestinal microbial communities between the two species.
[1] |
方卫东, 鲁康乐, 张春晓, 等. 豆粕替代鱼粉对牛蛙生长、体组成、消化酶活力及肝脏生化指标的影响[J]. 水产学报, 2016, 40(11): 1742-1752.
|
[2] |
刘世伟. 稻蛙共作对稻田土壤生态及水稻生长、产量与品质性状的影响[D]. 广州: 华南农业大学, 2019: 8.
|
[3] |
牛树辉, 李红燕, 潘厚军, 等. 鲜活饵料和人工配合饲料对鳜肌肉营养成分和质构特性的影响[J]. 水生生物学报, 2023, 47(1): 37-44.
|
[4] |
JIANG W D, WU P, TANG R J, et al. Nutritive values, flavor amino acids, healthcare fatty acids and flesh quality improved by manganese referring to up-regulating the antioxidant capacity and signaling molecules TOR and Nrf2 in the muscle of fish[J]. Food Res Int, 2016, 89: 670-678. doi: 10.1016/j.foodres.2016.09.020
|
[5] |
何志刚, 王冬武, 徐永福, 等. 黑斑蛙肌肉营养成分分析及评价[J]. 中国饲料, 2018(17): 74-77.
|
[6] |
刘芳彬, 王伟, 黄永春, 等. 棘胸蛙肌肉营养成分季节变化分析与评价[J]. 食品工业科技, 2022, 43(9): 365-371.
|
[7] |
刘丽, 刘楚吾, 林东年, 等. 泰国虎纹蛙与中国虎纹蛙肌肉的营养成分比较[J]. 水利渔业, 2008(3): 64-66.
|
[8] |
蓝蔚青, 杜金涛, 刘大勇, 等. 3种养殖蛙类肌肉基本营养成分分析[J]. 广东海洋大学学报, 2022, 42(2): 142-147.
|
[9] |
WANG A R, RAN C, RINGO E, et al. Progress in fish gastrointestinal microbiota research[J]. Rev Aquac, 2018, 10(3): 626-640. doi: 10.1111/raq.12191
|
[10] |
何志刚, 伍远安, 徐永福, 等. 野生与养殖黑斑蛙肌肉营养品质的比较分析[J]. 水产科学, 2019, 38(4): 506-513.
|
[11] |
刘文舒, 郭小泽, 陈彦良, 等. 养殖黑斑蛙肠道微生物结构与功能分析[J]. 经济动物学报, 2021, 25(2): 68-76.
|
[12] |
刘文舒, 陈彦良, 郭小泽, 等. 虎纹蛙肠道微生物结构与功能分析[J]. 野生动物学报, 2020, 41(1): 171-181.
|
[13] |
刘亚秋, 刘明典, 李新辉, 等. 3个广东鲂地理种群肌肉营养成分及能量密度比较研究[J]. 南方水产科学, 2022, 18(4): 163-169.
|
[14] |
XIA Y, YU E M, LU M X, et al. Effects of probiotic supplementation on gut microbiota as well as metabolite profiles within Nile tilapia, Oreochromis niloticus[J]. Aquaculture, 2020, 527: 735428. doi: 10.1016/j.aquaculture.2020.735428
|
[15] |
韩迎雪, 林婉玲, 杨少玲, 等. 15种淡水鱼肌肉脂肪含量及脂肪酸组成分析[J]. 食品工业科技, 2018, 39(20): 217-222.
|
[16] |
陈明勇, 彭剑林, 李灿鹏. 双团棘胸蛙(Paa yunnanensis)肌肉营养成分分析与评价[J]. 云南大学学报(自然科学版), 2010, 32(1): 114-117.
|
[17] |
李晋南, 王良, 王连生. 水产动物异亮氨酸营养研究进展[J]. 动物营养学报, 2022, 34(3): 1374-1387. doi: 10.3969/j.issn.1006-267x.2022.03.002
|
[18] |
ZHANG S H, YANG Q, REN M, et al. Effects of isoleucine on glucose uptake through the enhancement of muscular membrane concentrations of GLUT1 and GLUT4 and intestinal membrane concentrations of Na+/glucose co-transporter 1 (SGLT-1) and GLUT2[J]. Br J Nutr, 2016, 116(4): 593-602. doi: 10.1017/S0007114516002439
|
[19] |
魏佳, 王壮壮, 于海波, 等. 产L-苏氨酸重组大肠杆菌的构建和发酵性能[J]. 微生物学通报, 2019, 46(4): 695-706.
|
[20] |
王莉苹, 陈飞, 韩雨哲, 等. 支链氨基酸(BCAA)及水产动物对其需求量的研究进展[J]. 饲料工业, 2015, 36(14): 35-40.
|
[21] |
赵亭亭, 陈超, 邵彦翔. 雌雄条纹锯鮨肌肉营养成分的比较与评价[J]. 渔业科学进展, 2019, 40(3): 151-159.
|
[22] |
卢文麒, 许琪娅, 蔡国鹤, 等. 不同规格花鳗鲡肌肉营养成分比较分析[J]. 饲料工业, 2023, 44(14): 80-86. doi: 10.13302/j.cnki.fi.2023.14.013
|
[23] |
张茜, 王瑞, 于智超, 等. 棕榈酸诱导胰岛β细胞氧化应激和内质网应激的研究进展[J]. 激光生物学报, 2023, 32(2): 118-125.
|
[24] |
徐庆, 张新胜, 张永, 等. 辛酸、癸酸和硬脂酸对ApoE~(-/-)小鼠肠道中外源性胆固醇吸收的影响[J]. 卫生研究, 2018, 47(1): 119-122, 127.
|
[25] |
SIRI-TARINO P W, CHIU S, BERGERON N, et al. Saturated fats versus polyunsaturated fats versus carbohydrates for cardiovascular disease prevention and treatment[J]. Annu Rev Nutr, 2015, 35(1): 517-543. doi: 10.1146/annurev-nutr-071714-034449
|
[26] |
张瑞, 白云鹏, 贾莉, 等. 牛至精油对平凉红牛半腱肌肉品质、脂肪酸及挥发性风味物质的影响[J]. 动物营养学报, 2022, 34(7): 4452-4463.
|
[27] |
梁琍, 桂庆平, 冉辉, 等. 野生与养殖黄颡鱼鱼卵的营养成分比较[J]. 水产科学, 2016, 35(5): 522-527.
|
[28] |
胡磊, 谢庆超, 潘迎捷, 等. 蒸制和煮制对中华绒螯蟹中胆固醇和脂肪酸含量的影响[J]. 上海海洋大学学报, 2023, 32(3): 649-659.
|
[29] |
吴娜. 基于脂质热氧化降解解析中华绒螯蟹关键香气物质的形成机制[D]. 上海: 上海海洋大学, 2017: 44-73.
|
[30] |
周礼敬, 沈东霞, 詹会祥. 鱼类肌肉营养成分与人体健康研究[J]. 畜牧与饲料科学, 2013, 34(5): 69-71.
|
[31] |
郭俊涛, 谢遇春, 苏馨, 等. 大足黑山羊不同部位肌肉脂肪酸比较研究[J]. 肉类研究, 2020, 34(9): 6-11.
|
[32] |
杜宁宁, 黄晓丽, 郝其睿, 等. 八种鲑科鱼类肌肉营养成分分析与营养价值评价[J]. 水产学杂志, 2022, 35(4): 9-18, 37.
|
[33] |
董立学, 喻亚丽, 毛涛, 等. 池塘内循环流水养殖斑点叉尾鮰肌肉品质的分析[J]. 中国水产科学, 2021, 28(7): 914-924.
|
[34] |
CHENG J H, SUN D W, HAN Z, et al. Texture and structure measurements and analyses for evaluation of fish and fillet freshness quality: a review[J]. Comp Rev Food Sci Food Saf, 2014, 13(1): 52-61. doi: 10.1111/1541-4337.12043
|
[35] |
KUEBUTORNYE F K A, WANG Z, LU Y, et al. Effects of three host-associated Bacillus species on mucosal immunity and gut health of Nile tilapia, Oreochromis niloticus and its resistance against Aeromonas hydrophila infection[J]. Fish Shellfish Immunol, 2020, 97: 83-95. doi: 10.1016/j.fsi.2019.12.046
|
[36] |
KHOSRAVI S, RAHIMNEJAD S, HERAULT M, et al. Effects of protein hydrolysates supplementation in low fish meal diets on growth performance, innate immunity and disease resistance of red sea bream Pagrus major[J]. Fish Shellfish Immunol, 2015, 45(2): 858-868. doi: 10.1016/j.fsi.2015.05.039
|
[37] |
林宜锦, 杨欢, 林鹏志, 等. 饵料中添加肉桂醛对日本鳗鲡肠道结构的影响[J]. 饲料研究, 2022, 45(11): 52-55.
|
[38] |
邵闯, 秦守旺, 朱波, 等. 投喂策略对牛蛙蝌蚪生长发育及肝肠功能的影响[J]. 水产学报, 2023, 47(10): 72-83.
|
[39] |
王健, 王玲, 鲁康乐, 等. 摄食全豆粕蛋白饲料的牛蛙肠道微生物组成[J]. 集美大学学报(自然科学版), 2018, 23(4): 241-248.
|
[40] |
江昀, 胡亚洲, 向建国, 等. 不同健康状态的棘胸蛙肠道菌群结构分析[J]. 水生生物学报, 2022, 46(9): 1332-1340.
|
[41] |
LIU Z, LIU W S, RAN C, et al. Abrupt suspension of probiotics administration may increase host pathogen susceptibility by inducing gut dysbiosis[J]. Sci Rep, 2016, 6(1): 23214. doi: 10.1038/srep23214
|
[42] |
张美玲, 单承杰, 杜震宇. 益生菌与鱼类肠道健康研究进展[J]. 水产学报, 2021, 45(1): 147-157.
|
[43] |
包瑞璇, 周皓隆, 方再郗, 等. 大菱鲆肠道乳杆菌Lactobacillus sp. N5的筛选鉴定及其抑制水产病原菌的研究[J]. 水产学杂志, 2023, 36(3): 38-45.
|
[44] |
雷喜玲, 叶金云, 陈丽仙, 等. 屎肠球菌的作用机制及其在水产动物中的应用[J]. 饲料工业, 2022, 43(22): 9-15.
|
[45] |
王腾腾. 一株许氏平鲉肠道乳酸菌的分离鉴定、特性分析及其应用研究[D]. 上海: 上海海洋大学, 2017: 31-37.
|
[46] |
韩琳. 一例日本对虾暴发性死亡的病原分析及益生菌联用对对虾免疫增强作用[D]. 上海: 上海海洋大学, 2017: 21-36.
|
[47] |
WANG L, WANG J, LU K L, et al. Total replacement of fish meal with soybean meal in diets for bullfrog (Lithobates catesbeianus): effects on growth performance and gut microbial composition[J]. Aquaculture, 2020, 524: 735236. doi: 10.1016/j.aquaculture.2020.735236
|
[1] | ZHANG Gongjun, YANG Changping, LIU Yan, ZHOU Wenli, SHAN Binbin, WANG Liangming, WANG Sihan, SUN Dianrong, JIA Chunbin. Pattern of fish community and its relationship with environmental factors in Fangchenggang (Qinzhou coastal area of Beibu Gulf)[J]. South China Fisheries Science, 2022, 18(4): 20-33. DOI: 10.12131/20210255 |
[2] | GONG Yuyan, XIAO Yayuan, XU Shannan, LIU Yong, YANG Yutao, HUANG Zirong, LI Chunhou. Zooplankton community structure in Hailing Bay and its relationship with primary environmental factors[J]. South China Fisheries Science, 2019, 15(6): 49-55. DOI: 10.12131/20180220 |
[3] | WANG Yanfeng, HU Qiwei, YU Jing, CHEN Pimao, SHU Liming. Effect assessment of fishery resources proliferation in Zhelin Bay marine ranching in eastern Guangdong[J]. South China Fisheries Science, 2019, 15(2): 12-19. DOI: 10.12131/20180143 |
[4] | MA Huan, QIN Chuanxin, CHEN Pimao, LIN Huijie, DUAN Dingyu. Valuation of ecosystem service of marine ranching in Zhelin Bay[J]. South China Fisheries Science, 2019, 15(1): 10-19. DOI: 10.12131/20180041 |
[5] | MA Huan, QIN Chuanxin, CHEN Pimao, FENG Xue, YUAN Huarong, LI Xiaoguo, LIN Huijie. Study of biomass carbon storage in Zhelin Bay marine ranch of South China Sea[J]. South China Fisheries Science, 2017, 13(6): 56-64. DOI: 10.3969/j.issn.2095-0780.2017.06.007 |
[6] | YUAN Huarong, CHEN Pimao, QIN Chuanxin, LI Xiaoguo, ZHOU Yanbo, FENG Xue, YU Jing, SHU Liming, TANG Zhenzhao, TONG Fei. Seasonal variation of fish community structure in Zhelin Bay, the South China Sea[J]. South China Fisheries Science, 2017, 13(2): 26-35. DOI: 10.3969/j.issn.2095-0780.2017.02.004 |
[7] | TONG Fei, QIN Chuanxin, YU Jing, CHEN Pimao. Ecological basis assessment of artificial reef site selection in Liuniu coastal waters of Zhelin Bay, Eastern Guangdong[J]. South China Fisheries Science, 2016, 12(6): 25-32. DOI: 10.3969/j.issn.2095-0780.2016.06.004 |
[8] | LIAO Xiuli, CHEN Pimao, MA Shengwei, CHEN Haigang. Community structure of phytoplankton and its relationship with environmental factors before and after construction of artificial reefs in Yangmeikeng, Daya Bay[J]. South China Fisheries Science, 2013, 9(5): 109-119. DOI: 10.3969/j.issn.2095-0780.2013.05.017 |
[9] | GU Yangguang, LIN Qin, WANG Zenghuang, MA Shengwei, LU Tengteng, KE Changliang, DU Feiyan. Spatial distribution and potential ecological risk assessment of heavy metals in Zhelin Bay sediments and its vicinity, China[J]. South China Fisheries Science, 2013, 9(2): 32-37. DOI: 10.3969/j.issn.2095-0780.2013.02.006 |
[10] | JIANG Zengjie, FANG Jianguang, MEN Qiang, WANG Wei. Studies on the interaction between shellfish long-line culture and environment in Sungo Bay[J]. South China Fisheries Science, 2006, 2(1): 23-29. |
1. |
李珊,高延奇,赵立宁,王莎,陈善真. 丁香酚和2-苯氧乙醇对不同品系罗非鱼的麻醉效果比较. 水产学杂志. 2025(02): 64-73 .
![]() | |
2. |
宋霖,陈小江,高鹏. 常温下三卡因和丁香酚对条纹小鲃的麻醉效果研究. 水产科学. 2024(01): 144-151 .
![]() | |
3. |
吴冰,董秀金,徐一力,许建和. 丁香酚慢性麻醉对红鲫血液生理及肝脏hsp70和hsp90mRNA表达量的影响. 河北渔业. 2024(05): 17-21 .
![]() | |
4. |
董秀金,徐一力,吴冰,许建和. 鱼类麻醉保活运输研究进展. 河北渔业. 2024(06): 41-46 .
![]() | |
5. |
谢希尧,区又君,徐淑玉,林欣,李加儿,肖裕杰. MS-222和丁香酚胁迫对四指马鲅幼鱼鳃及肝脏组织微观结构的影响. 广东农业科学. 2024(05): 144-154 .
![]() | |
6. |
黄琼叶,张建明,朱友芳,许秋龙. 丁香酚对细点石斑鱼麻醉效果及肝脏生理生化指标的影响. 河北渔业. 2024(10): 24-29 .
![]() | |
7. |
周烨,骆小年,段友健,李姣,罗来幸,曾雪,刘佳欣. 2种麻醉剂对马口鱼幼鱼麻醉效果研究. 水产科学. 2024(06): 965-973 .
![]() | |
8. |
吕曼,董义超,李蒙,于会国,郭建东,石英. MS-222对墨瑞鳕麻醉效果及血清生化指标的影响. 浙江海洋大学学报(自然科学版). 2024(04): 352-358 .
![]() | |
9. |
谢希尧,区又君,徐淑玉,林欣,李加儿,肖裕杰. 丁香酚对四指马鲅幼鱼的麻醉效果及抗氧化影响. 中国渔业质量与标准. 2024(04): 1-13 .
![]() | |
10. |
马凯,朱晓磊,张澜澜,卫明亮,赵成,何宝全,尹家胜,张永泉. 黑龙江茴鱼对几种常用消毒药物和麻醉剂的耐受性. 水产学杂志. 2024(06): 38-44 .
![]() | |
11. |
唐忠林,张佳佳,周国勤,陈树桥,徐钢春,徐跑,强俊,王佩佩. 丁香酚对“优鲈3号”幼鱼运输水质及其血液、肌肉生理指标的影响. 水产科技情报. 2023(01): 44-52 .
![]() | |
12. |
韦政坤,董宏标,赵文,陈飞,张传镶,陈健,宫保华,朱长波,张家松. 两种麻醉剂对凡纳滨对虾麻醉效果及组织氧化损伤的影响. 南方水产科学. 2023(01): 136-146 .
![]() | |
13. |
胡望娇,王翠华,冯广朋,庄平,郑跃平,季强. 丁香酚和MS-222对松江鲈的麻醉效果. 海洋渔业. 2023(02): 181-190 .
![]() | |
14. |
林晨,吴敏,王凯,曾三妹,吴佳雯,钟莉萍,凌淞江. QuEChERS-超高压液相色谱-串联质谱法同时测定水产品中的6种丁香酚类麻醉剂. 食品科学. 2023(08): 324-329 .
![]() | |
15. |
章霞,徐志进,李伟业,殷小龙,王易帆,陈爽,马雪彬. MS-222对大黄鱼幼鱼麻醉效果、组织结构及抗氧化酶活性的影响. 大连海洋大学学报. 2023(02): 267-274 .
![]() | |
16. |
陈军平,武慧慧,沈方方,张佳鑫,于若梦,付永杰,赵道全,谢国强,孙文喜. MS-222与丁香酚对黄尾鲴麻醉效果的比较. 湖北农业科学. 2023(08): 133-139 .
![]() | |
17. |
陈欣怡,王好学,徐开达,李鹏飞,周永东. 丁香酚对褐菖鲉的麻醉效果. 广东海洋大学学报. 2023(06): 146-152 .
![]() | |
18. |
李娇妮,廖秀睿,李曦,吕布,杨守国,臧战,VASQUEZ Herbert Ely,王爱民,郑兴,顾志峰. 运输方式对马氏珠母贝(Pinctada fucata)稚贝存活率、生长、消化及抗氧化性能的影响探究. 海洋与湖沼. 2022(02): 414-420 .
![]() | |
19. |
戴晓娜,陆亦宽,蔡杨杨,孙虹,卢瑛,谢晶. 基于磁性纳米探针的渔用麻醉剂三卡因的快速检测方法. 食品与发酵工业. 2022(08): 245-253 .
![]() | |
20. |
朱晓玲,张菊,刘杰,韩智,吴婉琴,张莉,王会霞,江丰. 湖北省水产品兽药残留状况分析. 食品安全质量检测学报. 2021(01): 69-77 .
![]() | |
21. |
谢晶,曹杰. 渔用麻醉剂在鱼类麻醉保活运输中应用的研究进展. 上海海洋大学学报. 2021(01): 189-196 .
![]() | |
22. |
胡发文,王晓龙,高凤祥,李莉,菅玉霞,王雪,樊英,潘雷,郭文. 温度、盐度和两种麻醉剂对大泷六线鱼幼鱼耗氧率、排氨率的影响. 海洋科学. 2021(01): 54-61 .
![]() | |
23. |
刘阳,贾贵芳,杨飞,李忠利. 麻醉剂MS-222对运输胁迫黄颡鱼抗氧化系统的影响. 安徽农学通报. 2021(05): 67-69 .
![]() | |
24. |
陈旭,左涛,周胜杰,杨蕊,于刚,秦传新,马振华. 尖吻鲈幼鱼运输密度和时长对运输水质及其复苏率和抗氧化能力的影响. 南方水产科学. 2021(02): 122-128 .
![]() | |
25. |
曹杰,王琪,梅俊,谢晶. 有水与无水保活运输对大菱鲆生理应激及鱼肉品质的影响. 水产学报. 2021(07): 1034-1042 .
![]() | |
26. |
王维鑫,崔爱君,徐永江,李海宁,柳学周,王滨,姜燕,刘新富. MS-222和丁香油对黄条(鱼师)麻醉效果研究. 渔业科学进展. 2021(06): 205-214 .
![]() | |
27. |
曹杰,谢晶. 间氨基苯甲酸乙酯甲磺酸盐在大菱鲆模拟保活运输中的作用效果. 食品科学. 2021(23): 246-253 .
![]() | |
28. |
王金林,王万良,张忭忭,谭大明. MS-222和丁香酚对亚东鲑鱼麻醉效果的比较. 甘肃农业大学学报. 2021(06): 26-32+40 .
![]() | |
29. |
管敏,张德志,饶军,唐大明. 温度对中华鲟(Acipenser sinensis)幼鱼麻醉效果的影响. 生态学杂志. 2020(02): 576-585 .
![]() | |
30. |
朱之发,边力,刘心田,常青,阮晓红,陈四清,李凤辉,葛建龙,刘长琳. 12种麻醉剂对长蛸的麻醉效果. 中国水产科学. 2020(02): 195-203 .
![]() | |
31. |
黄小林,杨洁,戴超,李涛,杨育凯,黄忠,虞为,林黑着,孙莘溢,舒琥. 丁香酚对紫红笛鲷幼鱼的麻醉效果. 中国渔业质量与标准. 2020(02): 51-58 .
![]() | |
32. |
陈小江,熊俐灵,吴建顾,亓鲁,王权. MS-222和丁香酚对四川华吸鳅耗氧率和排氨率的影响. 南方水产科学. 2020(04): 69-74 .
![]() | |
33. |
王文豪,董宏标,孙彩云,段亚飞,李华,刘青松,张家松,曾祥兵. 石菖蒲挥发油和水溶性氮酮对鱼用麻醉剂的促皮渗透效果研究. 南方水产科学. 2020(04): 62-68 .
![]() | |
34. |
杨洁,朱晓玲. 丁香酚在水产品中的残留及风险评估研究进展. 食品安全质量检测学报. 2020(18): 6523-6529 .
![]() |