Volume 13 Issue 5
Oct.  2017
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FU Xiaozhe, FANG Wei, LIN Qiang, LIU Lihui, LIANG Hongru, HUANG Zhibin, XU Dehai, LI Ningqiu. Immune response of mandarin fish (Siniperca chuatsi) to attenuated Flavobacterium johnsoniae vaccine[J]. South China Fisheries Science, 2017, 13(5): 33-38. DOI: 10.3969/j.issn.2095-0780.2017.05.005
Citation: FU Xiaozhe, FANG Wei, LIN Qiang, LIU Lihui, LIANG Hongru, HUANG Zhibin, XU Dehai, LI Ningqiu. Immune response of mandarin fish (Siniperca chuatsi) to attenuated Flavobacterium johnsoniae vaccine[J]. South China Fisheries Science, 2017, 13(5): 33-38. DOI: 10.3969/j.issn.2095-0780.2017.05.005
shu

Immune response of mandarin fish (Siniperca chuatsi) to attenuated Flavobacterium johnsoniae vaccine

  • 1.

    Key Lab. of Fishery Drug Development, Ministry of Agriculture; Key Lab. of Aquatic Animal Immune Technology, Guangdong Province; Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

  • 2.

    Guangdong Provincial Aquatic Animal Epidemic Disease Prevention and Control Center, Guangzhou 510220, China

  • 3.

    3Aquatic Animal Health Research Unit, USDA, Auburn AL36832, USA

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  • Received Date: September 26, 2016
  • Revised Date: January 16, 2017
  • Accepted Date: April 16, 2017
    Graphical Abstract
    • Abstract

  • To study the immune response of mandarin fish (Siniperca chuatsi) to attenuated Flavobacterium johnsoniae strain M170, we used fluorescence quantitative RT-PCR method for determination of liver lysozyme (Lys) gene in spleen, gene expression dynamics of immunoglobulin (IgM), and detected the changes of the Lys activity and the antibody titer in serum. The results show that after the F.johnsoniae attenuated accine immunization, the Lys gene expression in liver increased significantly (P < 0.05), reaching the peak at the 48th hour, 10.9 times higher than that of the control group (P < 0.05), and had been higher than the control group significantly until the 28th day (P < 0.05). Meanwhile, the Lys activity in serum increased significantly, reaching the peak on the 2nd day, 4.35 times higher than that of the control group (P < 0.05) and was equivalent to the level of the control group on the 6th day. The relative expression of IgM gene from mandarin fish spleen began to increase and had been significantly higher than that of the control group since the 7th day (P < 0.05), 98.7 times higher than that of the control group until the 28th day (P < 0.05). The antibody titer in the immune fish serum was higher than that of the control group significantly since the 7th day (P < 0.05), reaching the peak (26) on the 28th day. On the 28th day after immunization, the strain M168 was used to attack the virus, and the relative survival rate of the immunized group was higher than that of the control group significantly, and the relative percentage survival (RPS) was 85.9%. The results show that the specific and non-specific immune response can be induced by attenued F.johnsoniae M170 and provide good protection for mandarin fish.

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