Volume 16 Issue 3
Jun.  2020
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MA Li, WU Jinying, GAO Songze, SUN Caiyun, LI Wensheng. Study on immunoprotection of eukaryotic expression plasmids of Streptococcus iniae simA and pgmA in Oreochromis niloticus[J]. South China Fisheries Science, 2020, 16(3): 38-46. DOI: 10.12131/20190163
Citation: MA Li, WU Jinying, GAO Songze, SUN Caiyun, LI Wensheng. Study on immunoprotection of eukaryotic expression plasmids of Streptococcus iniae simA and pgmA in Oreochromis niloticus[J]. South China Fisheries Science, 2020, 16(3): 38-46. DOI: 10.12131/20190163
shu

Study on immunoprotection of eukaryotic expression plasmids of Streptococcus iniae simA and pgmA in Oreochromis niloticus

  • School of Life Sciences, Sun Yat-sen University/Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals/Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Guangzhou 510275, China

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  • Received Date: August 19, 2019
  • Revised Date: January 05, 2020
  • Accepted Date: February 12, 2020
  • Available Online: February 20, 2020
    Graphical Abstract
    • Abstract

  • Nile tilapia (Oreochromis niloticus) is an important commercial fish in aquaculture in the world. However, it is easy to be infected by Streptococcus iniae in the breeding production. The use of vaccine is a relatively ideal anti infection measure. In this study, eukaryotic expression vectors (DNA vaccine) were constructed by taking the simA and pgmA genes of S. iniae as the target genes. Then protective efficacy was evaluated by intramuscular injection of tilapia. Two target genes were detected in the injected fish at DNA and RNA levels after immunization. Besides, 7−28 d  after the first immunization, the expression levels of interleukin-1 (IL-1β) and Tumor Necrosis Factor (TNF-α) in the gill, liver, kidney and head kidney of the vaccinal group were higher to some extent than those in the PBS control group. The antibody titer and antibacterial activity of immunized groups were significantly higher than those of control groups (P<0.05). Two weeks after being challenged with S. iniae, the relative percent survival of mixed vaccines of pcDNA3.1-pgmA, pcDNA3.1-simA and pcDNA3.1-pgmA, with pcDNA3.1-simA were 60.7%, 49.9% and 75.0%, respectively. It is revealed that the vaccine prepared in this paper has immune protection effect and can be used as a candidate vaccine.

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  • [1]
    MISHRA A, NAM G H, GIM J A, et al. Current challenges of Streptococcus infection and effective molecular, cellular, and environmental control methods in aquaculture[J]. Mol Cells, 2018, 41(6): 495-505.
    [2]
    GAUTHIER D T. Bacterial zoonoses of fishes: a review and appraisal of evidence for linkages between fish and human infections[J]. Vet J, 2015, 203(1): 27-35. doi: 10.1016/j.tvjl.2014.10.028
    [3]
    WEINBERGER D M, TRZCIŃSKI K, LU Y J, et al. Pneumococcal capsular polysaccharide structure predicts serotype prevalence[J]. PLoS Pathog, 2009, 5(6): e1000476. doi: 10.1371/journal.ppat.1000476
    [4]
    LOUIS L, WEINER D B. Rapid synthetic DNA vaccine development for emerging infectious disease outbreaks[M]//ROBERTSON E S. Microbiome and cancer. Current Cancer Research. Cham: Humana Press, 2019: 347-362.
    [5]
    COELHO-CASTELO A A, TROMBONE A P, ROSADA R S, et al. Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular delivery[J]. Genet Vaccines Ther, 2006, 4(1): 1-10. doi: 10.1186/1479-0556-4-1
    [6]
    NILSSON M, GIVSKOV M, TWETMAN S, et al. Inactivation of the pgmA gene in Streptococcus mutans significantly decreases biofilm-associated antimicrobial tolerance[J]. Microorganisms, 2019, 7(9): 310. doi: 10.3390/microorganisms7090310
    [7]
    SHENG X, GAO J, LIU H, et al. Recombinant phosphoglucomutase and CAMP factor as potential subunit vaccine antigens induced high protection against Streptococcus iniae infection in flounder (Paralichthys olivaceus)[J]. J Appl Microbiol, 2018, 125(4): 997-1007. doi: 10.1111/jam.13948
    [8]
    FISCHETTI V A. Surface proteins on Gram-positive bacteria [M]//FISCHETTI1V A, NOVICK R P, FERRETTI J J, et al. Gram-positive pathogens. Washington D. C.: ASM Press, 2019:19-31.
    [9]
    BAIANO J C F, TUMBOL R A, UMAPATHY A, et al. Identification and molecular characterisation of a fibrinogen binding protein from Streptococcus iniae[J]. BMC Biol, 2008, 8(1): 67-83. doi: 10.1186/1471-213X-8-67
    [10]
    KIM D, BECK B R, LEE S M, et al. Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing simA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus)[J]. Fish Shellfish Immunol, 2016, 55: 374-383. doi: 10.1016/j.fsi.2016.06.010
    [11]
    杨成良, 李从荣. 海豚链球菌类M样蛋白基因突变株BY-1ΔsimA的免疫保护作用[J]. 检验医学, 2017, 32(7): 647-651. doi: 10.3969/j.issn.1673-8640.2017.07.021
    [12]
    ANDERSON D P. Adjuvants and immunostimulants for enhancing vaccine potency in fish[J]. Dev Biol Stand, 1997, 90: 257-265.
    [13]
    王雷, 李光友, 毛远兴. 中国对虾血淋巴中的抗菌、溶菌活力与酚氧化酶活力的测定及其特性研究[J]. 海洋与湖沼, 1995, 26(2): 179-185. doi: 10.3321/j.issn:0029-814X.1995.02.010
    [14]
    BOMAN H G, NILSSON-FAYE I, PAUL K, et al. Insect immunity. I. Characteristics of an inducible cell-free antibacterial reaction in hemolymph of Samia cynthia pupae[J]. Infect Immun, 1974, 10(1): 136-145. doi: 10.1128/IAI.10.1.136-145.1974
    [15]
    徐增辉. 罗非鱼海豚链球菌疫苗的研制及免疫效果的初步研究[D]. 南宁: 广西大学, 2008: 47.
    [16]
    CHEN X R, LI L, HU Q B, et al. Expression of dsRNA in recombinant Isaria fumosorosea strain targets the TLR7 gene in Bemisia tabaci[J]. BMC Biotechnol, 2015, 15(1): 64-72. doi: 10.1186/s12896-015-0170-8
    [17]
    TONHEIM T C, LEIRVIK J, LØVOLL M, et al. Detection of supercoiled plasmid DNA and luciferase expression in Atlantic salmon (Salmo salar L.) 535 days after injection[J]. Fish Shellfish Immunol, 2007, 23(4): 867-876. doi: 10.1016/j.fsi.2007.03.015
    [18]
    SETERNES T, TONHEIM T C, LØVOLL M, et al. Specific endocytosis and degradation of naked DNA in the endocardial cells of cod (Gadus morhua L.)[J]. J Exp Biol, 2007, 210(Pt 12): 2091-2103.
    [19]
    张择扬. 四种鸡球虫DNA疫苗在机体组织中的分布和在环境中的释放[D]. 南京: 南京农业大学, 2011: 1.
    [20]
    王蓓, 简纪常, 蔡双虎, 等. 罗非鱼无乳链球菌DNA疫苗的构建及免疫效果研究[J]. 中国海洋大学学报(自然科学版), 2013, 43(12): 30-35.
    [21]
    EMBREGTS C W E, RIGAUDEAU D, TACCHI L, et al. Vaccination of carp against SVCV with an oral DNA vaccine or an insect cells-based subunit vaccine[J]. Fish Shellfish Immunol, 2019, 85: 66-77. doi: 10.1016/j.fsi.2018.03.028
    [22]
    GAM A A, NEVA F A, KROTOSKI W A. Comparative sensitivity and specificity of ELISA and IHA for serodiagnosis of strongyloidiasis with larval antigens[J]. Am J Trop Med Hyg, 1987, 37(1): 157-161. doi: 10.4269/ajtmh.1987.37.157
    [23]
    ALISHAHI M, MOJTABA R A, ZAREI M, et al. Effect of dietary chitosan on immune response and disease resistance in Cyprinus carpio[J]. Iran J Vet Med, 2014, 8(2): 125-133.
    [24]
    DINARELLO C A. Interleukin-1[J]. Rev Infect Dis, 1984, 6(1): 51-95. doi: 10.1093/clinids/6.1.51
    [25]
    TONHEIM T C, BØGWALD J, DALMO R A. What happens to the DNA vaccine in fish? A review of current knowledge[J]. Fish Shellfish Immunol, 2008, 25(1/2): 1-18. doi: 10.1016/j.fsi.2008.03.007
    [26]
    MA Y P, KE H, LIANG Z L, et al. Protective efficacy of cationic-PLGA microspheres loaded with DNA vaccine encoding the sip gene of Streptococcus agalactiae in tilapia[J]. Fish Shellfish Immunol, 2017, 66: 345-353. doi: 10.1016/j.fsi.2017.05.003
    [27]
    HÉLÈNE M, JEAN-CHRISTOPHE G, EMILIE R, et al. How to design the surface of peptide-loaded nanoparticles for efficient oral bioavailability?[J]. Adv Drug Deliv Rev, 2016, 106(Pt B): 320-336.
    [28]
    HADY E L, WALAA E A. In vitro-in vivo evaluation of chitosan-PLGA nanoparticles for potentiated gastric retention and anti-ulcer activity of diosmin[J]. Int J Nanomed, 2019, 14: 7191-7213. doi: 10.2147/IJN.S213836
    [29]
    杨爱琼, 谢勇恩. 结核杆菌多价核酸疫苗在小鼠体内的免疫保护效应研究[J]. 川北医学院学报, 2015, 30(5): 579-582. doi: 10.3969/j.issn.1005-3697.2015.05.01
    [30]
    郑颖. 犬联合多价核酸疫苗的实验免疫研究[J]. 中国人兽共患病学报, 2016, 32(4): 406-411. doi: 10.3969/j.issn.1002-2694.2016.04.017
    [31]
    KAYANSAMRUAJ P, DONG H T, PIRARAT N, et al. Efficacy of α-enolase-based DNA vaccine against pathogenic Streptococcus iniae in Nile tilapia (Oreochromis niloticus)[J]. Aquaculture, 2017, 468: 102-106. doi: 10.1016/j.aquaculture.2016.10.001
    [32]
    LIU C, HU X, CAO Z, et al. Construction and characterization of a DNA vaccine encoding the SagH against Streptococcus iniae[J]. Fish Shellfish Immunol, 2019, 89: 71-75. doi: 10.1016/j.fsi.2019.03.045
    [33]
    INTHASAENG P, UNAJAK S, AREECHON N, et al. Efficacy of pcDNA-Alp1 DNA vaccine against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus)[J]. Thai J Vet Med, 2018, 48(2): 279-288.
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