Volume 18 Issue 6
Dec.  2022
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JIA Huining, SHI Miaomiao, BIAN Yongle, SHI Chongjing, LIU Hengwei, SONG Xuehong, QIN Fenju. Effects of nanometer selenium on immune protection and antioxidant ability of Eriocheir sinensis under hypoxia stress[J]. South China Fisheries Science, 2022, 18(6): 100-109. DOI: 10.12131/20220106
Citation: JIA Huining, SHI Miaomiao, BIAN Yongle, SHI Chongjing, LIU Hengwei, SONG Xuehong, QIN Fenju. Effects of nanometer selenium on immune protection and antioxidant ability of Eriocheir sinensis under hypoxia stress[J]. South China Fisheries Science, 2022, 18(6): 100-109. DOI: 10.12131/20220106
shu

Effects of nanometer selenium on immune protection and antioxidant ability of Eriocheir sinensis under hypoxia stress

  • 1.

    School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou 215009, China

  • 2.

    School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China

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  • Received Date: April 18, 2022
  • Revised Date: June 05, 2022
  • Accepted Date: June 13, 2022
  • Available Online: June 23, 2022
    Graphical Abstract
    • Abstract
  • Hypoxia stress will weaken the immune function and antioxidant capacity of Eriocheir sinensis. In order to reveal the immune protection and anti-oxidation regulation mechanism of nanometer selenium (nano-Se) on E. sinensis under hypoxia stress, we had fed E. sinensis with different doses of nano-Se (0, 0.1, 0.2, 0.4, 0.8 and 1.6 mg·kg−1) in basic diets for 90 d. After the feeding, we conducted a hypoxia stress test and injected Aeromonas hydrophila under hypoxia stress. The results show that: 1) The mortality of E. sinensis under hypoxia stress for 24 h and that infected with A. hydrophila under hypoxia stress reached 62.45% and 100%, respectively. The levels of hemocyanin and the hemocyte count in crab hemolymph, and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in crab tissues increased significantly under hypoxia stress for 12 h (P<0.05), as well as there was a decreasing trend under stress to 24 h. The contents of lactic acid (LD) and malondialdehyde (MDA) continued to rise from 12 to 24 h under hypoxia stress. 2) Appropriate addition amount (0.1−0.4 mg·kg−1) of nano-Se reduced the mortality of E. sinensis significantly and the lethality of A. hydrophila under hypoxia stress (P<0.05), increasing the levels of hemocyanin, the hemocyte count and the activities of antioxidant enzymes (SOD, CAT, GSH-Px) under hypoxia stress significantly, but decreasing the contents of LD and MDA (P<0.05). The addition of 0.8−1.6 mg·kg−1 nano-Se had aggravated hypoxia stress injury. These results indicate that appropriate addition of nano-Se to the diets can improve the decrease of immune response and antioxidant ability of E. sinensis under hypoxia stress, and the optimal dose of nano-Se in basal diets is 0.2 mg·kg−1.
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