Citation: | NIU Gaigai, DENG Jianchao, LI Laihao, YANG Xianqing, DENG Yongchuan, WU Yanyan, HAO Shuxian, QI Bo. Extraction of flavonoids from Canavalia maritima by accelerated solvent extraction[J]. South China Fisheries Science, 2014, 10(1): 78-85. DOI: 10.3969/j.issn.2095-0780.2014.01.012 |
Based on a single-factor analysis, we used Response Surface Methodology (RSM) to optimize the method for extraction of flavonoids by accelerated solvent extraction (ASE) technology from Canavalia maritima. The extraction temperature, static extraction time and methanol concentration were chosen as independent variables and the flavonoids extraction rate was selected as response value; Box-Behnken method was adopted for the designment of the experiment to study the significance of each factor and its mutual influence on extraction rate. Then we utilized Design Expert software to establish the mathematical model of polynomial quadratic equation. The optimum conditions of flavonoids extraction were as follows: 130 ℃ of extraction temperature, 16 min of extraction time, 66% of methanol concentration and static circulate twice. The extraction rate was 83.43% at the optimum conditions and the relative error between the actual and predicted values was only 1.63%.
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