Volume 15 Issue 6
Dec.  2019
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PEI Ruonan, ZHAI Honglei, QI Bo, YANG Xianqing. Optimization of multi-enzymatic extraction of polysaccharide from Gelidium amansii by response surface methodology[J]. South China Fisheries Science, 2019, 15(6): 88-95. DOI: 10.12131/20190081
Citation: PEI Ruonan, ZHAI Honglei, QI Bo, YANG Xianqing. Optimization of multi-enzymatic extraction of polysaccharide from Gelidium amansii by response surface methodology[J]. South China Fisheries Science, 2019, 15(6): 88-95. DOI: 10.12131/20190081
shu

Optimization of multi-enzymatic extraction of polysaccharide from Gelidium amansii by response surface methodology

  • 1.

    National R & D Center for Aquatic Product Processing; Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

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  • Received Date: April 07, 2019
  • Revised Date: June 18, 2019
  • Accepted Date: June 27, 2019
  • Available Online: July 02, 2019
    Graphical Abstract
    • Abstract

  • To optimize the multi-enzymatic extraction process of polysaccharides from Gelidium amansii, we designed a three-factor and three-level Box-Benhnken center-united experiment based on single factor test, and conducted a regression analysis. The optimum technological parameters of enzymatic extraction of G. amansii polysaccharide were: enzymes addition 2.30%, enzymatic hydrolysis temperature 60.43 ℃, ratio of material to water 1∶32 (g·mL−1). The extraction yield of polysaccharide was up to 15.98% after 120 min of enzymatic hydrolysis under these conditions.

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    • References (34)
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