Changes of biogenic amines in liquid-smoked tilapia stored at 25 ℃

In order to study the changes of biogenic amines (BA) in liquid-smoked tilapia slices during storage, the samples were stored in normal and vacuum packages at 25 ℃ to observe the changes of putrescine, tryptamine, histamine, phenethylamine and spermidine by HPLC-UV detector. The results indicate that the five BAs can be effectively separated within 30 min, and have a good linear relation when concentration is in the range of 0.25~10 gmL-1 (R20. 999); their lowest limit of detection (LOD) is 0.02 gmL-1 and the recoveries range from 73.8 % to 113.6 %; the RSDs are less than 10%. In vacuum package and normal package, the final mass fraction of putrescine are (13.940.66) and (16.250.16) mgkg-1, respectively; histamine, (8.760.17) and (6.380.21 )mgkg-1, respectively; spermidine, (0.850.17) and (0.950.21 )mgkg-1, respectively. However, tryptamine and phenethylamine can not be detected during the storage. Vacuum package can effectively delay and inhibit the increase of putrescine, but does not affect histamine and spermidine. Additionally, the histamine content in both two packages does not exceed the national standard.