Volume 9 Issue 4
Aug.  2013
Turn off MathJax
Article Contents
Abstract
References
LUO Fangfang, QIAN Zhuozhen, LIN Rongxiao, WU Chengye. Determination of colistin sulfate, bacitracin and virginiamycin M1 residues in aquatic products by HPLC-MS/MS[J]. South China Fisheries Science, 2013, 9(4): 62-68. DOI: 10.3969/j.issn.2095-0780.2013.04.011
Citation: LUO Fangfang, QIAN Zhuozhen, LIN Rongxiao, WU Chengye. Determination of colistin sulfate, bacitracin and virginiamycin M1 residues in aquatic products by HPLC-MS/MS[J]. South China Fisheries Science, 2013, 9(4): 62-68. DOI: 10.3969/j.issn.2095-0780.2013.04.011
shu

Determination of colistin sulfate, bacitracin and virginiamycin M1 residues in aquatic products by HPLC-MS/MS

  • 1.

    College of Biology Engineering, Jimei University, Xiamen 361021, China

  • 2.

    Fisheries Research Institute of Fujian, Xiamen 361012, China

  • 3.

    Fujian Agriculture and Forestry University, Fuzhou 350002, China

More Information
  • Received Date: October 11, 2012
  • Revised Date: December 28, 2012
    Graphical Abstract
    • Abstract

  • A high performance liquid chromatography tandem mass spectrometric (HPLC-MS/MS) method was established to simultaneously detect colistin sulfuric (CS), bacitracin (BTC) and virginiamycin M1 (VGMM1) in aquatic products. We extracted the samples with methanol-0.1% formicacid(V : V=2 : 5), removed their protein with 4% TCA acetonitril, removed fat with acetonitrile saturated n-hexane, and then cleaned them up with OASiS HLB solid phase extraction (SPE) column. Next, we detected the analytes by HPLC-MS/MS under the selected reaction monitoring mode and quantified them by external standard method. Results show that CS and BTC have good linearity between the peak areas in concentrations ranged 0.01~10.00 mg · L-1, VGMM1 as well in concentrations ranged 0.002~2.000 mg · L-1, and the correlation coefficients (R2) are all more than 0.995. The detection limits of CS, BTC and VGMM1 are 10 μg · kg-1, 10 μg · kg-1and 2 μg · kg-1 respectively, and the quantification limits are 20 μg · kg-1, 20 μg · kg-1 and 4 μg · kg-1respectively. Three samples in three different concentration levels are chosen for recovery, getting a 72.3%~103.9% average recovery rate, and a relative standard deviations varying from 1.10%~10.92%. In conclusion, method proposed has advantages of simplicity, sensitivity and repeatability, which provide technical support for the detection of these 3 kinds of drug residues in aquatic products.

    • FullText(HTML)
    • References (17)
  • [1]
    MOATS W A, LESKINEN L. Determination of virginiamycin residues in swine tissue using high performance liquid chromatography[J]. J Agric Food Chem, 1988, 36(6) : 1297-1300. doi: 10.1021/jf00084a043
    [2]
    耿志明, 陈明, 许大光, 等. 高效液相色谱法测定猪组织中维吉尼亚霉素M1的残留[J]. 中国兽药杂志, 2005, 39(2): 10-13. doi: 10.3969/j.issn.1002-1280.2005.02.005
    [3]
    孙兴权, 李哲, 林维宣. 动物源性食品中多肽类抗生素残留检测技术研究进展[J]. 中国食品卫生杂志, 2008, 20(3): 263-266. doi: 10.3969/j.issn.1004-8456.2008.03.021
    [4]
    中华人民共和国农业部. 中华人民共和国农业部公告第235号动物性食品中兽药最高残留限量[EB/OL]. (2002-12-24)[2012-06-24]. http://www.moa.gov.cn/zwllm/tzgg/gg/200302/t20030226_59300.html"> http://www.moa.gov.cn/zwllm/tzgg/gg/200302/t20030226_59300.html.
    [5]
    MASCHER D G, UNGER C P, MASCHER H J. Determination of neomycin and bacitracin in human or rabbit serum by HPLC-MS/MS[J]. J Pharmaceutical Biomed Anal, 2007, 43(2): 691-700. doi: 10.1016/j.jpba.2006.08.008
    [6]
    MA Z, WANG J P, GERBER J P, et al. Determination of colistin in human plasma, urine and other biologica samples using LC-MS/MS[J]. J Chromatogr B, 2008, 862(1/2): 205-212. doi: 10.1016/j.jchromb.2007.12.009
    [7]
    陈小霞, 岳振峰, 叶卫翔, 等. 液相色谱串联质谱法快速测定牛奶与鸡蛋中的维吉霉素M1残留量[J]. 分析测试学报, 2009, 28(11): 1328-1331. doi: 10.3969/j.issn.1004-4957.2009.11.022
    [8]
    林维宣, 孙兴权, 田苗, 等. 动物组织中粘杆菌素、杆菌肽及维吉尼霉素残留量的液相色谱-串联质谱检测[J]. 分析测试学报, 2009, 28(2): 212-215. doi: 10.3969/j.issn.1004-4957.2009.02.018
    [9]
    JANSSON B, KARVANEN M, CARS O, et al. Quantitative analysis of colistin A and colistin B in plasma and culture medium using a simple precipitation step followed by LC/MS/MS[J]. J Pharmaceut Biomed Anal, 2009, 49(3): 760-767. doi: 10.1016/j.jpba.2008.12.016
    [10]
    苏萌, 艾连峰, 段文仲, 等. 高效液相色谱-串联质谱法测定动物源食品中粘杆菌素、多粘菌素B的残留量[J]. 分析试验室, 2012, 31(3): 73-77. doi: 10.3969/j.issn.1000-0720.2012.03.019
    [11]
    魏云计, 冯民, 刘艳, 等. 液相色谱-电喷雾电离三重四级杆质谱测定畜禽肝脏中维吉尼亚霉素M1的残留量[J]. 分析试验室, 2012, 31(4): 50-53.
    [12]
    任义广, 曲志勇, 王家春, 等. 液相色谱-质谱/质谱快速测定饲料中杆菌肽方法的研究[J]. 广州饲料, 2010, 19(10): 28-30. doi: 10.3969/j.issn.1005-8613.2010.10.009
    [13]
    黄永辉. 超高效液相色谱-串联质谱法测定饲料中的维吉尼霉素M1[J]. 色谱, 2011, 29(10): 962-966. doi: 10.3724/SP.J.1123.2011.00962
    [14]
    WAN C H, HO C, SIN D W, et al. Detection of residual bacitracin A, colistin A and colistin B in milk and animal tissues by liquid chromatography tandem mass spectrometry[J]. Anal Bioanal Chem, 2006, 385 (1): 181-188. doi: 10.1007/s00216-006-0325-5
    [15]
    林维宣, 孙兴权, 田苗. 液相色谱-串联质谱法检测牛乳中多肽类抗生素残留量[J]. 中国乳品工业, 2009, 37(3): 46-48. doi: 10.3969/j.issn.1001-2230.2009.03.012
    [16]
    刘佳佳, 金芬, 佘永新, 等. 液相色谱-串联质谱法测定牛奶中5种多肽类抗生素[J]. 分析化学研究报告, 2011, 39(5): 652-657. doi: 10.3724/SP.J.1096.2011.00652
    [17]
    刘琪, 孙雷, 张骊. 牛奶中粘菌素和杆菌肽残留的固相萃取超高效液相色谱-串联质谱法测定[J]. 中国兽药杂志, 2011, 45(7): 17-20. doi: 10.3969/j.issn.1002-1280.2011.07.006
    • Related Articles

  • Cited by

    Periodical cited type(2)

    1. 钱静, 沈和定, 管菊. 平疣桑椹石磺生殖系统结构及精子储存场所. 动物学杂志. 2015(04): 600-606 .
    2. 钱静, 沈和定, 管菊, 王成暖. 两种石磺胚胎发育比较. 上海海洋大学学报. 2014(04): 498-504 .

    Other cited types(1)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(3)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return