Citation: | CHEN Chen, HAO Shuxian, CEN Jianwei, HUANG Hui, ZHAO Yongqiang, WEI Ya, YANG Shaoling. Impact of low-temperature acclimation on antioxidant capacity and gilltissue structure of Micropterus salmoides[J]. South China Fisheries Science. DOI: 10.12131/20240297 |
Low-temperature acclimation is critical for live fish transportation to reduce injuries and mortality by inducing the fish into semi-hibernate state. Thus, we investigated the suitable holding temperature for largemouth bass (Micropterussalmoides) transportation, focusing on the effect of water temperature on its muscle tissue, serum biochemical indicators, antioxidant enzyme activities and respiratory function. The results show that the respiratory rate of M. salmoides graduallydecreased as temperature descended. Significant differences were observed in the muscle tissue, pH, serum biochemical indicators, and antioxidant capacity between temperatures (15 ℃ and 20 ℃) and lower temperatures (9 ℃ and 12 ℃) (p<0.05)during holding of 48-hour. M. salmoides exposed semi-hibernate state at 12 ℃, which led to a significant lower quality of total ammonia nitrogen (TAN) accumulation in the water (p<0.05) and a slower TAN increase rate. In the 12 ℃ group, the serum glucose (GLU) concentration and lactate dehydrogenase (LDH) activity decreased significantly, while the total antioxidant capacityincreased, with no significant changes on other indicators (p>0.05), and the blood cells in the gill tissue arranged orderly, with gill filaments showing no obvious deformation. The findings indicate that 12 ℃ holding not only effectively maintains water quality,but also effectively preserves the physiological functions and antioxidant capacity of fish, thereby reducing damage caused byoxidative stress reactions. Therefore, it is recommended to control the temperature of the water body at around 12 ° C during the actual holding and circulation process.
[1] |
乔娣, 雷宁, 朱俊杰, 等. 大口黑鲈幼鱼肝脏抗大口黑鲈弹状病毒应答的转录组分析[J]. 南方水产科学, 2024, 20(4): 164-176.
|
[2] |
SUN H, DONG W J, HE G L, et al. Excessive level of dietaryinsect protein negatively changed growth metabolomic and transcriptomic profiles of largemouth bass (Micropterus salmoides) [J]. Anim. Nutr, 2024, 17(2): 397-407.
|
[3] |
袁仲瑾, 岑剑伟, 李来好, 等. 低温暂养对珍珠龙胆石斑鱼存活、非特异性免疫及抗氧化指标的影响[J]. 南方水产科学, 2022, 18(6): 118-126.
|
[4] |
范秀萍, 秦小明, 章超桦, 等. 温度对有水保活石斑鱼代谢与鱼肉品质的影响[J]. 农业工程学报, 2018, 34(14): 241-248.
|
[5] |
王洪臣, 于金国. 水温对鱼类的作用与影响[J]. 黑龙江水产, 2011(3): 30-31.
|
[6] |
ONUKWUFOR O J, KAMUNDE C. Interactive effects of temperature, cadmium, and hypoxia on rainbow trout (Oncorhynchus mykiss) liver mitochondrial bioenergetics[J]. Eco Environ Saf, 2024, 289: 117450.
|
[7] |
NAM S E, HAQUE M N, SHIN Y K, et al. Constant and intermittent hypoxia modulates immunity, oxidative status, and blood components of red seabream and increases its susceptibility to the acute toxicity of red tide dinoflagellate[J]. Fish Shellfish Immun, 2020, 105: 286-296. doi: 10.1016/j.fsi.2020.07.030
|
[8] |
SALES F C, SANTOS D E P K, RIZZO E, et al. Proliferation, survival and cell death in fish gills remodeling: from injury to recovery[J]. Fish Shellfish Immunol, 2017, 68: 10-18. doi: 10.1016/j.fsi.2017.07.001
|
[9] |
MOLTUMYR L, GISMERVIK S, GU J, et al. Does the thermal component of warm water treatment inflict acute lesions onAtlantic salmon (Salmo salar)?[J]. Aquaculture, 2021, 532: 736048. doi: 10.1016/j.aquaculture.2020.736048
|
[10] |
胡玲红, 王映, 王化敏, 等. 不同温度胁迫对青鳉鳃凋亡的影响[J]. 大连海洋大学学报, 2021, 36(6): 929-936.
|
[11] |
谢玉净, 李胜杰, 杜金星, 等. 不同大口黑鲈群体耐热性分析及高温对鳃组织和抗氧化酶活性的影响[J/OL]. [2025-01-10].http://kns.cnki.net/kcms/detail/31.1283.s.20241203.0942.004.html.
|
[12] |
何静怡, 郑伟, 黄卉, 等. 不同温度、盐度条件对草鱼暂养及应激保活的影响[J]. 大连海洋大学学报, 2024, 39(4): 597-605.
|
[13] |
刘伟, 郑天伦, 孟庆辉, 等. 温度对大口黑鲈的影响及养殖生产中的应用[J]. 水产养殖, 2021, 42(4): 56-57, 59.
|
[14] |
MORRO B, BALSEIRO P, ALBALAT A, et al. Effects of temperature and photoperiod on rainbow trout (Oncorhynchus mykiss) smoltification and haematopoiesis[J]. Aquaculture, 2020, 519: 734711. doi: 10.1016/j.aquaculture.2019.734711
|
[15] |
MARTA M, CAROLINE C, YANNICK R, et al. Effects of warming rate, acclimation temperature and ontogeny on the critical thermal maximum of temperate marine fish larvae[J]. PLoS One, 2017, 12(7): e0179928. doi: 10.1371/journal.pone.0179928
|
[16] |
严凌苓, 陈婷, 龙映均, 等. 国内外水产品保鲜技术研究进展[J]. 江西水产科技, 2013(2): 38-41.
|
[17] |
ANNA B, KATHRYN C, DANIEL J B, et al. Respiration of mesopelagic fish: a comparison of respiratory electron transport system (ETS) measurements and allometrically calculated rates in the southern ocean and benguela current[J]. ICES J Mar Sci, 2020, 77(5): 1672-1684. doi: 10.1093/icesjms/fsaa031
|
[18] |
WHITE P D, WAHL H D. Growth and physiological responses in largemouth bass populations to environmental warming: effects of inhabiting chronically heated environments[J]. J Therm Biol, 2020, 88: 102467. doi: 10.1016/j.jtherbio.2019.102467
|
[19] |
魏孟申, 郑涛, 路思琪, 等. 氨氮胁迫对大口黑鲈幼鱼组织结构、酶活及肠道微生物的影响[J]. 水生生物学报, 2024, 48(1): 10-22.
|
[20] |
JIANG X J, DONG S L, LIU R X, et al. Effects of temperature,dissolved oxygen, and their interaction on the growth performance and condition of rainbow trout (Oncorhynchus mykiss)[J]. J Therm Biol, 2021, 98: 102928. doi: 10.1016/j.jtherbio.2021.102928
|
[21] |
常向阳, 姜沛宏, 邓杰, 等. 冷胁迫诱导休眠方式对虾夷扇贝无水保活期生命特征及营养品质指标的影响[J]. 南方水产科学, 2023, 19(3): 129-139.
|
[22] |
BJØRNEVIK M, KARLSEN Ø, JOHNSTON A I, et al. Effect of sustained exercise on white muscle structure and flesh quality in farmed cod (Gadus morhuaL.)[J]. J Aquat Food Prod Technol, 2003, 34(1): 55-64.
|
[23] |
REFAEY M M, TIAN X, TANG R, et al. Changes in physiological responses, muscular composition and flesh quality of channel catfish ictalurus punctatus suffering from transport stress[J]. Aquaculture, 2017, 478: 9-15. doi: 10.1016/j.aquaculture.2017.01.026
|
[24] |
YANG S, WU H, HE K, et al. Response of AMP-activated protein kinase and lactate metabolism of largemouth bass (Micropterus salmoides) under acute hypoxic stress[J]. Sci Total Environ, 2019, 666: 1071-1079. doi: 10.1016/j.scitotenv.2019.02.236
|
[25] |
LEI C X, XIE Y J, SONG H M, et al. Different responses toglucose overload between two strains of largemouth bass (Micropterus salmoides) [J]. Front Physiol, 2022, 13: 1010633. doi: 10.3389/fphys.2022.1010633
|
[26] |
刘佳林. 水中添加柠檬酸对大口黑鲈耐低氧能力的影响[J]. 水产科学, 2024, 31(5): 576-587.
|
[27] |
LAVAJOO F, PERELLÓ A M, VÉLEZ E J, et al. Regulatory mechanisms involved in muscle and bone remodeling during refeeding in gilthead sea bream[J]. Sci Rep, 2020, 10: 184. doi: 10.1038/s41598-019-57013-6
|
[28] |
崔雁娜, 徐磊, 郭水荣, 等. 暂养净化对池塘养殖大口黑鲈营养成分及挥发性风味物质的影响[J]. 食品安全质量检测学报, 2023, 14(16): 94-103.
|
[29] |
赵曼曼, 熊光权, 石柳, 等. 低氧胁迫对鱼肉品质影响机制的研究进展[J]. 食品科学, 2021, 42(21): 292-298.
|
[30] |
BARTON A B. Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteroids[J]. Integr Comp Biol, 2002, 42(3): 517-525. doi: 10.1093/icb/42.3.517
|
[31] |
廖磊, 刘浩, 赵柳兰, 等. 腹腔注射葡萄糖对大口黑鲈血糖稳态和糖代谢关键酶活性的影响[J]. 淡水渔业, 2022, 52(3): 105-112.
|
[32] |
陈晓瑛, 陈绍坚, 黄文, 等. 菌酶协同发酵豆粕替代鱼粉对大口黑鲈生长性能、血清生化、免疫和抗氧化指标及肝脏组织形态的影响[J]. 动物营养学报, 2021, 33(5): 2848-2863.
|
[33] |
YU J J, ZHONG D B, LI S, et al. Acute temperature stressestrigger liver transcriptome and microbial community remodeling in largemouth bass (Micropterus salmoides)[J]. Aquaculture, 2023, 573: 739573. doi: 10.1016/j.aquaculture.2023.739573
|
[34] |
黄嘉, 冉旭东, 刘欣平, 等. 胆汁酸和牛磺酸对大口黑鲈生长、肝脏健康及肠道屏障的影响[J]. 水产学报, 2024, 48(9): 38-48.
|
[35] |
PHILIP M A, VIJA M M. Stress-immune-growth interactions: cortisol modulates suppressors of cytokine signaling and JAK/STAT pathway in rainbow trout liver[J]. PLoS One, 2015, 10(6): e0129299. doi: 10.1371/journal.pone.0129299
|
[36] |
李江涛, 张艳秋, 张虹, 等. 密度胁迫对大口黑鲈游泳行为及肌肉能量代谢的影响[J]. 南方水产科学, 2024, 20(2): 102-110.
|
[37] |
陆健, 张佳佳, 周国勤, 等. 急性高温胁迫对大口黑鲈“优鲈3号”组织损伤及HSPs基因表达的影响[J]. 水产科学, 2021, 40(4): 508-515.
|
[38] |
杨斯琪, 郑洪武, 孙颖, 等. 氨氮、温度和体重对大口黑鲈(Micropterus salmoides) 幼鱼耗氧率和窒息点的影响[J]. 海洋与湖沼, 2019, 50(6): 1328-1333.
|
[39] |
LIU Y H, ZHAO Y, ZHU D, et al. 1, 8-cineole and ginger extract (Zingiber officinale Rosc) as stress mitigator for transportation of largemouth bass (Micropterus salmoides L.)[J]. Aquaculture, 2022, 561: 738622. doi: 10.1016/j.aquaculture.2022.738622
|
[40] |
强俊, 徐跑, 何杰, 等. 氨氮与拥挤胁迫对吉富品系尼罗罗非鱼幼鱼生长和肝脏抗氧化指标的联合影响[J]. 水产学报, 2011, 35(12): 1837-1848.
|
[41] |
曹善茂, 王潇, 刘钢, 等. 温度胁迫对岩扇贝幼贝抗氧化酶活力的影响[J]. 大连海洋大学学报, 2018, 33(2): 223-227.
|
[42] |
张美东, 凌晨, 沙航, 等. 低氧-复氧胁迫对鲢抗氧化酶活性及Cu/Zn-SOD和Mn-SOD基因表达的影响[J]. 水生生物学报, 2022, 46(4): 498-506.
|
[43] |
李豫, 黄建盛, 陈有铭, 等. 低温胁迫对军曹鱼幼鱼鳃组织抗氧化能力、细胞凋亡和组织结构的影响[J]. 南方水产科学, 2023, 19(3): 68-77.
|
[44] |
SUN J L, ZHAO L L, LIAO L, et al. Interactive effect of thermal and hypoxia on largemouth bass (Micropterus salmoides) gill and liver: aggravation of oxidative stress, inhibition of immunity and promotion of cell apoptosis[J]. Fish Shellfish Immunol, 2020, 98: 923-936. doi: 10.1016/j.fsi.2019.11.056
|
[45] |
MOHSEN A, MONIER M N, HOSEIN S H, et al. Fish response to hypoxia stress: growth, physiological, and immunological biomarkers[J]. Fish Physiol Biochem, 2019, 45(3): 997-1013. doi: 10.1007/s10695-019-00614-9
|
[46] |
LI X H, LING C, WANG Q X, et al. Hypoxia stress induces tissue damage, immune defense, and oxygen transport change in gill of silver carp (Hypophthalmichthys molitrix): evaluation on hypoxia by using transcriptomics[J]. Front Mar Sci, 2022, 9: 900200. doi: 10.3389/fmars.2022.900200
|
[47] |
JIN J H, AMENOYOGBE E, YANG Y, et al. Effects of ammonia nitrogen stress on the physiological, biochemical, and metabolic levels of the gill tissue of juvenile four-finger threadfin (Eleutheronema tetradactylum)[J]. Aquat Toxicol, 2024, 274: 107049. doi: 10.1016/j.aquatox.2024.107049
|
[1] | HU Xiaona, WU Xingbing, ZHU Yongjiu, CHEN Siqi, SHI Zechao, ZHANG Yuansong, WANG Long, LI Xiaoli, LI Xuemei. Effects of stocking density on growth performance and physiological and biochemical parameters of Pelteobagrus vachelli juvenile[J]. South China Fisheries Science. DOI: 10.12131/20240266 |
[2] | QIAN Zhenjia, XU Jincheng, LIU Huang, CUI Mingchao, ZHANG Chenglin. Effects of flow velocity on growth performance and physiological and biochemical indexes of large yellow croaker (Larimichthys crocea) in welfare aquaculture[J]. South China Fisheries Science. DOI: 10.12131/20250036 |
[3] | QIU Yuyan, ZHANG Zhiyong, CHEN Shuyin, NI Kewen, JIA Chaofeng, MENG Qian, ZHU Fei, ZHANG Zhiwei, TANG Xiaojian. Comparative study on feeding frequency of hybrid F2 of Acanthopagrus schlegelii ♀ × Pagrus major♂ and A. schlegelii[J]. South China Fisheries Science, 2022, 18(1): 59-67. DOI: 10.12131/20210081 |
[4] | ZENG Xiangbing, DONG Hongbiao, WEI Zhengkun, DUAN Yafei, CHEN Jian, ZHANG Hui, SUN Caiyun, XU Xiaodong, ZHANG Jiasong. Effects of polysaccharide from Endothelium corneum gigeriae galli on growth, digestive, intestinal antioxidant capacity and serum biochemical indices of Lates calcarifer[J]. South China Fisheries Science, 2021, 17(4): 49-57. DOI: 10.12131/20210028 |
[5] | YU Wei, YANG Yukai, LIN Heizhao, HUANG Xiaolin, HUANG Zhong, LI Tao, ZHOU Chuanpeng, MA Zhenhua, XUN Pengwei, YANG Changping. Effects of taurine on growth performance, digestive enzymes, antioxidant capacity and immune indices of Lateolabrax maculatus[J]. South China Fisheries Science, 2021, 17(2): 78-86. DOI: 10.12131/20200223 |
[6] | YE Penghao, HAN Tingting, FU Guiquan, GU Yangguang, HUANG Honghui. Physiological response of Sargassum hemiphyllum to cadmium stress[J]. South China Fisheries Science, 2019, 15(5): 35-40. DOI: 10.12131/20190032 |
[7] | YU Wei, YANG Yukai, CHEN Zhibin, LIN Heizhao, HUANG Xiaolin, ZHOU Chuanpeng, YANG Keng, CAO Yucheng, HUANG Zhong, MA Zhenhua, LI Tao, WANG Jun, WANG Yun, XUN Pengwei, HUANG Qianqian, YU Wanfeng. Dietary effect of Spirulina platensis on growth performance, digestive enzymes, haematological indices and antioxidant capacity of Chinese sea bass (Lateolabrax maculatus)[J]. South China Fisheries Science, 2019, 15(3): 57-67. DOI: 10.12131/20190002 |
[8] | ZHAO Shuyan, LIN Heizhao, HUANG Zhong, ZHOU Chuanpeng, WANG Jun, WANG Yun, QI Changle, YANG Xiaoli, LIAO Jingqiu. Effect of small peptide supplementation at different protein levels on growth performance, digestive enzymes activities, serum biochemical indices and antioxidant abilities of grouper (Epinephelus akaara)[J]. South China Fisheries Science, 2016, 12(3): 15-23. DOI: 10.3969/j.issn.2095-0780.2016.03.003 |
[9] | SU Hui, OU Youjun, LI Jia′er, WANG Yongcui, LIU Rujian, CAO Shouhua. Effects of starvation on antioxidative capacity, Na+/K+-ATPase activity and biochemical composition in juvenile Trachinotus ovatus[J]. South China Fisheries Science, 2012, 8(6): 28-36. DOI: 10.3969/j.issn.2095-0780.2012.06.005 |
[10] | YUAN Fenghua, LIN Heizhe, LI Zhuojia, LU Xin, YANG Qibin. Effects of dietary Bacillus licheniformis on blood physiological-biochemical indices in cultured Lates calarifer[J]. South China Fisheries Science, 2009, 5(2): 45-50. DOI: 10.3969/j.issn.1673-2227.2009.02.008 |