Volume 19 Issue 5
Oct.  2023
Turn off MathJax
Article Contents
Abstract
References
Related Articles
XIA Feiyu, ZHANG Xiumei, XU Pian, XU Ying, WANG Yihang. Comparative analysis of shell frame characteristics and mitochondrial 16S rRNA gene between wild and cultured mussels (Mytilus coruscus)[J]. South China Fisheries Science, 2023, 19(5): 168-176. DOI: 10.12131/20230096
Citation: XIA Feiyu, ZHANG Xiumei, XU Pian, XU Ying, WANG Yihang. Comparative analysis of shell frame characteristics and mitochondrial 16S rRNA gene between wild and cultured mussels (Mytilus coruscus)[J]. South China Fisheries Science, 2023, 19(5): 168-176. DOI: 10.12131/20230096
shu

Comparative analysis of shell frame characteristics and mitochondrial 16S rRNA gene between wild and cultured mussels (Mytilus coruscus)

  • Fishery College, Zhejiang Ocean University, Zhoushan 316022, China

More Information
  • Received Date: May 03, 2023
  • Revised Date: June 07, 2023
  • Accepted Date: June 25, 2023
  • Available Online: June 29, 2023
    Graphical Abstract
    • Abstract

  • Understanding the impact of the environment of Mytilus unguiculatus on its growth is extremely important for promoting high-quality development of mussel breeding industry. Taking the wild (From Gouqi intertidal area) and cultured (From mussel culture rafts) M. coruscus as research objects, we constructed 13 shell frame characteristics indexes based on shell length, including shell width and shell height to compare their main morphological differences, and sequenced their 16S rRNA gene to analyze the genetic and morphological associations between them. The results show that there were significant differences in ten shell frame characteristics between wild and cultured mussels (P<0.05), and the shells of wild mussels were generally wider, thicker and heavier. Four variables were selected by stepwise discrimination method: L1 (Shell width), L2 (Shell height), L10 (Distance from the top of shell to the upper end of hinge), L12 (Distance from the top of shell to the byssus orifice). The comprehensive discrimination accuracy of wild and cultured mussels was 94.9%. The 16S rRNA gene sequencing shows that the wild mussels nucleotide diversity (π) was 0.089, and haplotype diversity (Hd) was 0.894; cultured mussels' π was 0.087 and Hd was 0.682. The genetic diversity level of wild and cultured mussles were relatively high, but the phylogenetic tree and haplotype network prove that there was no significant genetic differentiation between them. It is speculated that their morphological differences might be mainly influenced by their habitat environments (Wave exposure, growth density, etc.).

    • FullText(HTML)
    • References (42)
  • [1]
    常抗美, 吴剑锋. 厚壳贻贝人工繁殖技术的研究[J]. 南方水产, 2007, 3(3): 26-30.
    [2]
    张义浩, 赵盛龙. 嵊山列岛贻贝养殖种类生长发育调查[J]. 浙江海洋学院学报 (自然科学版), 2003, 22(1): 67-73.
    [3]
    吴剑锋, 顾庆庭, 常抗美, 等. 厚壳贻贝人工育苗中幼虫合理培养密度的探讨[J]. 动物学杂志, 1982, 17(1): 19-22.
    [4]
    SÉBASTIEN L G, HAMILTON D J, BARBEAU M A. Effect of mussel density and size on the morphology of blue mussels (Mytilus edulis) grown in suspended culture in Prince Edward Island, Canada[J]. Aquaculture, 2005, 249(1/2/3/4): 265-274.
    [5]
    HOBDAY A. Body-size variation exhibited by an intertidal limpet: influence of wave exposure, tidal height and migratory behavior[J]. J Exp Mar Biol Ecol, 1995, 189(1/2): 29-45.
    [6]
    BOULDING E G, HOLST M, PILON V. Changes in selection on gastropod shell size and thickness with wave-exposure on northeastern pacific shores[J]. J Exp Mar Biol Ecol, 1999, 232(2): 217-239. doi: 10.1016/S0022-0981(98)00117-8
    [7]
    刘硕博, 唐祖蓉, 申望, 等. 基于F型mtDNA D-Loop的厚壳贻贝 (Mytilus coruscus) 群体遗传多样性研究[J]. 海洋与湖沼, 2019, 50(2): 355-364.
    [8]
    管成林, 李继姬, 郭宝英. 基于线粒体CO I基因比较分析野生与养殖厚壳贻贝种群的遗传多样性[J]. 安徽农业科学, 2012, 40(13): 7660-7662.
    [9]
    刘二田, 陈一, 战欣, 等. 企鹅珍珠贝微卫星标记与生长性状的相关性研究[J]. 水生生物学报, 2023, 47(2): 332-338.
    [10]
    YUAN Y, KANNO M, KIJIMA A. Genetic diversity of wild populations of Chlamys farreri in Japan and their genetic relationship with cultured stocks in China[J]. Aquaculture, 2012, 370: 109-122.
    [11]
    HE X, WU F C, QI H G, et al. Whole-genome resequencing reveals the single nucleotide polymorphisms associated with shell shape in Crassostrea gigas[J]. Aquaculture, 2022, 547: 737502. doi: 10.1016/j.aquaculture.2021.737502
    [12]
    徐义平, 许会宾, 金凯, 等. 浙江乐清湾缢蛏的形态和遗传多样性[J]. 上海海洋大学学报, 2017, 26(1): 31-37. doi: 10.12024/jsou.20151001575
    [13]
    程亮, 徐善良, 刘飞, 等. 厚壳贻贝性腺不同发育时期肥满度与生化成分分析[J]. 海洋学研究, 2013, 31(4): 68-73.
    [14]
    白晓倩, 杨阳, 邹李昶, 等. 嵊泗列岛海域三种贻贝贝体框架特征的差异[J]. 海洋与湖沼, 2014, 45(5): 1078-1084.
    [15]
    李思发, 李晨虹, 李家乐. 尼罗罗非鱼品系间形态差异分析[J]. 动物学报, 1998(4): 75-82.
    [16]
    SUDHIR K, GLEN S, KOICHIRO T. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Mol Biol Evol, 2016, 33(7): 1870-1874. doi: 10.1093/molbev/msw054
    [17]
    GREFSRUD E S, OIVIND S. Comparison of shell strength in wild and cultured scallops (Pecten maximus)[J]. Aquaculture, 2006, 251(2/3/4): 306-313.
    [18]
    顾志峰, 王嫣, 石耀华, 等. 马氏珠母贝两个不同地理种群的形态性状和贝壳珍珠质颜色比较分析[J]. 渔业科学进展, 2009, 30(1): 79-86.
    [19]
    张义浩. 浙江沿海贻贝种类形态比较研究[J]. 渔业经济研究, 2009(2): 14-20.
    [20]
    FRANK E. ANDERSON. Phylogeny and historical biogeography of the loliginid squids (Mollusca: Cephalopoda) based on mitochondrial DNA sequence data[J]. Mol Phylogenet Evol, 2000, 15(2): 191-214. doi: 10.1006/mpev.1999.0753
    [21]
    孙明媛, 朱锐, 孙晓晴, 等. 基于CO I和16S rRNA的库页岛马珂蛤遗传多样性和分子系统进化研究[J]. 中国水产科学, 2018, 25(4): 891-901.
    [22]
    闫永斌, 葛玉双, 程起群, 等. 基于线粒体16S rRNA和CO I序列的江苏启东海域4种贝类遗传多样性分析[J]. 海洋渔业, 2022, 44(2): 201-208. doi: 10.3969/j.issn.1004-2490.2022.02.007
    [23]
    谭月, 邱明, 李金龙, 等. 基于线粒体16S rRNA基因序列的中日朝菲律宾蛤仔野生群体遗传多样性分析[J]. 大连海洋大学学报, 2021, 36(1): 127-134.
    [24]
    SHEN Y B, LI J L, FENG B B. Genetic analysis of cultured and wild populations of Mytilus coruscus based on mitochondrial DNA[J]. Zool Res, 2009, 30(3): 240-246. doi: 10.3724/SP.J.1141.2009.03240
    [25]
    叶莹莹, 徐梅英, 郭宝英, 等. 我国东南沿海厚壳贻贝 (Mytilus coruscus) 4个群体线粒体16S rRNA序列及遗传结构分析[J]. 海洋与湖沼, 2012, 43(2): 376-381. doi: 10.11693/hyhz201202028028
    [26]
    MONTAUDOUIN X D, LUCIA M, BINIAS C, et al. Why is Asari (=Manila) clam Ruditapes philippinarum fitness poor in Arcachon Bay: a meta-analysis to answer?[J]. Estuar Coast Shelf Sci, 2016, 179(20): 226-235.
    [27]
    HAN Z Q, LI Q, LIU S K, et al. Genetic variability of an orange-shell line of the Pacific oyster Crassostrea gigas during artificial selection inferred from microsatellites and mitochondrial CO I sequences[J]. Aquaculture, 2019, 508: 159-166. doi: 10.1016/j.aquaculture.2019.04.074
    [28]
    SMKA B, AMSB C. Molecules and morphology reveal 'new' widespread North American freshwater mussel species (Bivalvia: Unionidae)[J]. Mol Phylogenet Evol, 2019(138): 182-192.
    [29]
    祁剑飞, 宁岳, 巫旗生, 等. 菲律宾蛤仔5个地理群体形态差异分析[J]. 应用海洋学学报, 2020, 39(2): 189-194. doi: 10.3969/J.ISSN.2095-4972.2020.02.005
    [30]
    董建宇, 胡成业, 杨晓龙, 等. 基于GLM模型Logistic回归的菲律宾蛤仔地理群体判别[J]. 海洋与湖沼, 2018, 49(6): 1318-1324. doi: 10.11693/hyhz20180100002
    [31]
    仉佳文, 唐君玮, 张远帆, 等. 皱纹盘鲍 (Haliotis discus hannai) 不同群体贝壳形态和相对生长变异研究[J]. 海洋与湖沼, 2022, 53(6): 1539-1547. doi: 10.11693/hyhz20220300050
    [32]
    MOESER G M, CARRINGTON E. Seasonal variation in mussel byssal thread mechanics[J]. J Exp Biol, 2006, 209(10): 1996-2003. doi: 10.1242/jeb.02234
    [33]
    STEFFANI C N, BRANCH G M. Growth rate, condition, and shell shape of Mytilus galloprovincialis: responses to wave exposure[J]. Mar Ecol Prog Ser, 2003, 246(6): 197-209.
    [34]
    AKESTER R J, MARTEL A L. Shell shape, dysodont tooth morphology, and hinge-ligament thickness in the bay mussel Mytilus trossulus correlate with wave exposure[J]. Can J Zool, 2000, 78(2): 240-253. doi: 10.1139/z99-215
    [35]
    FRCHETTE M, DESPLAND E. Impaired shell gaping and food depletion as mechanisms of asymmetric competition in mussels[J]. Ecoscience, 1999, 6(1): 1-11. doi: 10.1080/11956860.1999.11952200
    [36]
    杨冠林. 典型贻贝浮筏式养殖海域物理-生态-贝类生长耦合数值模型的构建及应用[D]. 上海: 上海海洋大学, 2022: 2-8.
    [37]
    温英. 贻贝浮筏养殖设施水流阻力的量化及其生态效应研究[D]. 上海: 上海海洋大学, 2021: 16-20.
    [38]
    FRÉCHETTE M, BOURGET E. Energy flow between the pelagic and benthic zones: factors controlling particulate organic matter available to an intertidal mussel bed[J]. Can J Fish Aquat Sci, 1985, 42(6): 1158-1165. doi: 10.1139/f85-143
    [39]
    WATANABE S, KATAYAMA S. Relationships among shell shape, shell growth rate, and nutritional condition in the Manila clam (Ruditapes philippinarum) in Japan[J]. J Shellfish Res, 2014, 29(2): 353-359.
    [40]
    李杰. 日本镜蛤 (Dosinorbis japonica) 数量性状、营养成分及幼体生态学研究[D]. 大连: 大连海洋大学, 2018: 23-30.
    [41]
    张学开, 范超, 霍忠明, 等. 不同群体长牡蛎壳型相关性状的比较分析[J]. 中国海洋大学学报 (自然科学版), 2021, 51(1): 1-9.
    [42]
    江勇波. 企鹅珍珠贝摄食生理能量学研究[D]. 海口: 海南大学, 2014: 12-14.
    • Related Articles

  • Related Articles

    [1]LI Wenjing, LI Chunsheng, WANG Yueqi, CHEN Shengjun, ZHAO Yongqiang, WU Yanyan, LI Laihao. Improvement effect of Halanaerobium fermentans YL9-2 on quality and flavor of fish sauce during fermentation[J]. South China Fisheries Science, 2022, 18(2): 115-123. DOI: 10.12131/20210314
    [2]ZHOU Kaimin, JIANG Shigui, HUANG Jianhua, YANG Qibin, JIANG Song, QIU Lihua, YANG Lishi, ZHOU Falin. Cloning and expression analysis of Chitinase-2 from Penaeus monodon during molting cycle and different larval developmental stages[J]. South China Fisheries Science, 2017, 13(4): 59-68. DOI: 10.3969/j.issn.2095-0780.2017.04.008
    [3]WANG Rui, LI Liping, HUANG Ting, LIANG Wanwen, LIANG Cong, LEI Aiying, CHEN Ming. Real-time quantitative PCR for detection of Streptococcus agalactiae from tilapia tissue[J]. South China Fisheries Science, 2015, 11(3): 41-46. DOI: 10.3969/j.issn.2095-0780.2015.03.007
    [4]QIAN Weiguo, YE Chao, WANG Weijie, LU Kexiang. Underwater irradiance and optimal allocation of 2 kW straight type fish aggregation lamps[J]. South China Fisheries Science, 2015, 11(2): 90-95. DOI: 10.3969/j.issn.2095-0780.2015.02.013
    [5]ZHANG Ning, SU Shengyan, DONG Zaijie, MA Qingnan, YUAN Xinhua. Study on bone and microstructure of common carp using micro CT scanning[J]. South China Fisheries Science, 2012, 8(6): 44-49. DOI: 10.3969/j.issn.2095-0780.2012.06.007
    [6]YU Guohui, CHEN Yanhong, CHENG Ping, LI Yongjian, YANG Zihong, CHEN Yuanfeng. Influence of several metal ions on growth and nitrite removal of Rhodopseudomonas palustris strain 2-8[J]. South China Fisheries Science, 2011, 7(4): 30-35. DOI: 10.3969/j.issn.2095-0780.2011.04.005
    [7]HUANG Haili, DU Xiaodong, ZHOU Yinhuan. Effects of feeding larvae and juveniles of Haliotis diversicolor with 2 benthic diatoms[J]. South China Fisheries Science, 2011, 7(1): 32-38. DOI: 10.3969/j.issn.2095-0780.2011.01.005
    [8]CHEN Lei, CHENG Yuanzhi, WANG Rixin, XU Tianjun. Cloning and sequence analysis of Cyt b gene in 2 Gobiidae fishes[J]. South China Fisheries Science, 2010, 6(5): 43-49. DOI: 10.3969/j.issn.1673-2227.2010.05.007
    [9]ZHOU Chen. Karyotype analysis of chromosome of 2 Gobioid species[J]. South China Fisheries Science, 2010, 6(4): 72-76. DOI: 10.3969/j.issn.1673-2227.2010.04.012
    [10]YU Da-hui, CHU ka-hou. Study on ITS 2 molecular markers of six pearl oyster species in the genus Pinctada[J]. South China Fisheries Science, 2005, 1(4): 6-12.

  • Cited by

    Periodical cited type(6)

    1. 陈志，翁朝红，邹丽珍，周洪磊，蒋奕雄，方垂弘，李裕红，关杰耀. 中国鲎人工驯养繁殖技术初步研究. 渔业研究. 2023(03): 246-253 .
    2. 钟赟民，翁朝红，王志婷，李佳怡，谢仰杰. 中国鲎室内人工繁育和鲎卵、幼体常见病原鉴定及其防治. 中国水产科学. 2023(06): 799-811 .
    3. 陈晓海，李银康，应紫薇，郭清扬，颉晓勇. 人为干扰栖息地退化对潮间带中华鲎幼鲎种群数量的影响. 生态学报. 2023(16): 6824-6832 .
    4. YING Ziwei，BAO Yuyuan，LI Yinkang，YE Guoling，ZHANG Shuhuan，XU Peng，ZHU Junhua，XIE Xiaoyong. Impact of Different Diets on Adult Tri-Spine Horseshoe Crab, Tachypleus tridentatus. Journal of Ocean University of China. 2022(03): 541-548 .
    5. 郭清扬，左娅，林吴颖，肖晓波，赵心忆，李银康，应紫薇，周传江，颉晓勇. 北部湾中华鲎（Tachypleus tridentatus）幼鲎生态位及种间竞争分析. 海洋学报. 2022(10): 109-118 .
    6. 应紫薇，李银康，颉晓勇. 中华鲎(Tachypleus tridentatus)稚鲎对不同比例泥沙类型选择行为特征. 海洋与湖沼. 2022(05): 1242-1249 .

    Other cited types(1)

Catalog

    Get Citation
    PDF
    XML
    Article views (315) PDF downloads (71) Cited by(7)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return