Volume 16 Issue 6
Dec.  2020
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ZHANG Linbao, CHEN Haigang, TIAN Fei, SUN Wei, ZHANG Zhe, CAI Wengui. Gender differences in neurotoxicity and oxidative damage of triazophos on Perna viridis[J]. South China Fisheries Science, 2020, 16(6): 75-80. DOI: 10.12131/20200097
Citation: ZHANG Linbao, CHEN Haigang, TIAN Fei, SUN Wei, ZHANG Zhe, CAI Wengui. Gender differences in neurotoxicity and oxidative damage of triazophos on Perna viridis[J]. South China Fisheries Science, 2020, 16(6): 75-80. DOI: 10.12131/20200097
shu

Gender differences in neurotoxicity and oxidative damage of triazophos on Perna viridis

  • South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of South China Sea Fishery Resources and Environment, Ministry of Agriculture and Rural Affairs/Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China

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  • Received Date: May 07, 2020
  • Revised Date: July 23, 2020
  • Accepted Date: August 13, 2020
  • Available Online: September 27, 2020
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    • Abstract

  • We studied the gender differences of the neurotoxicity and oxidative damage effects of triazophos on Perna viridis that has function of environmental indicator for the first time. The changes of acetylcholinesterase (AchE) and several antioxidant indices in gill tissues of male and female were determined after 15 d of chronic exposure to 35 μg·L−1 (1/100 96 h LC50). The results show that there was no significant change in the AchE activity in female's gill tissue after being treated with triazophos for 15 d, while the AchE activity in male was significantly inhibited. The activities of superoxide dismutase (SOD) and catalase (CAT) in gill tissues of both male and female were significantly inhibited, and the malondialdehyde (MDA) content was significantly increased. The activity of glutathione S-transferase (GST) and the content of reduced glutathione (GSH) showed significant gender differences. Only the GST activity and GSH content in male had significant changes. In conclusion, triazophos shows obvious neurotoxicity and oxidative damage effects on gill tissue of P. viridis, and the male was more sensitive to triazophos exposure.

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  • [1]
    钟志, 刘琴, 刘士忠, 等. 三唑磷对浙江沿岸海域水生生物的影响[J]. 广州化工, 2010, 38(5): 215-216. doi: 10.3969/j.issn.1001-9677.2010.05.080
    [2]
    ZHENG S, CHEN B, QIU X, et al. Distribution and risk assessment of 82 pesticides in Jiulong River and estuary in South China[J]. Chemosphere, 2016, 144: 1177-1192. doi: 10.1016/j.chemosphere.2015.09.050
    [3]
    张志勇, 何丹军, 徐敦名, 等. 三唑磷在水中消解和残留动态研究[J]. 现代农药, 2005, 4(1): 25-27. doi: 10.3969/j.issn.1671-5284.2005.01.009
    [4]
    WANG Y, CHEN C, ZHAO X, et al. Assessing joint toxicity of four organophosphate and carbamate insecticides in common carp (Cyprinus carpio) using acetylcholinesterase activity as an endpoint[J]. Pestic Biochem Phys, 2015, 122: 81-85. doi: 10.1016/j.pestbp.2014.12.017
    [5]
    LIU L, ZHU B, GONG Y X, et al. Neurotoxic effect of triazophos on goldfish (Carassius auratus) and tissue specific antioxidant responses[J]. Ecotox Environ Saf, 2015, 116: 68-75. doi: 10.1016/j.ecoenv.2015.03.001
    [6]
    ZHU B, GONGY X, LIU L, et al. Toxic effects of triazophos on rare minnow (Gobiocypris rarus) embryos and larvae[J]. Chemosphere, 2014, 108: 46-54. doi: 10.1016/j.chemosphere.2014.03.036
    [7]
    YANG F W, FANG B, PANG G F, et al. Organophosphorus pesticide triazophos: a new endocrine disruptor chemical of hypothalamus-pituitary-adrenal axis[J]. Pestic Biochem Phys, 2019, 159: 91-97. doi: 10.1016/j.pestbp.2019.05.021
    [8]
    WU S, LI X, LIU X, et al. Joint toxic effects of triazophos and imidacloprid on zebrafish (Danio rerio)[J]. Environ Pollut, 2018, 235: 470-481. doi: 10.1016/j.envpol.2017.12.120
    [9]
    WANG G, SHAO J, WU M, et al. Effect of acute exposure of triazophos on histological structure and apoptosis of the brain and liver of zebrafish (Danio rerio)[J]. Ecotoxicol Environ Saf, 2019, 180: 646-655. doi: 10.1016/j.ecoenv.2019.05.053
    [10]
    WANG G, XIONG D, WU M, et al. Induction of time-and dose-dependent oxidative stress of triazophos to brain and liver in zebrafish (Danio rerio)[J]. Comp Biochem Physiol C, 2019, 228: 108640.
    [11]
    徐广春, 顾中言, 徐德进, 等. 五种常用杀虫剂对灰飞虱繁殖力的影响[J]. 植物保护学报, 2008, 35(4): 361-366. doi: 10.3321/j.issn:0577-7518.2008.04.014
    [12]
    孙伟, 张林宝, 胡莹, 等. 三唑磷作用下翡翠贻贝抑制性消减杂交文库构建与分析[J]. 中国环境科学, 2016, 36(12): 3807-3815. doi: 10.3969/j.issn.1000-6923.2016.12.036
    [13]
    JI C, WU H, WEI L, et al. Proteomic and metabolomic analysis reveal gender-specific responses of mussel Mytilus galloprovincialis to 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE 47)[J]. Aquat Toxicol, 2013, 140-141: 449-457. doi: 10.1016/j.aquatox.2013.07.009
    [14]
    JI C, LI F, WANG Q, et al. An integrated proteomic and metabolomic study on the gender-specific responses of mussels Mytilus galloprovincialis to tetrabromobisphenol A (TBBPA)[J]. Chemosphere, 2016, 144: 527-539. doi: 10.1016/j.chemosphere.2015.08.052
    [15]
    LUO L, ZHANG Q, KONG X, et al. Differential effects of Zn exposure on male and female oysters (Crassostrea angulata) as revealed by label-free quantitative proteomics: differential effects of Zn exposure on male and female oysters[J]. Environ Toxicol Chem, 2017, 36(10): 2602-2613. doi: 10.1002/etc.3797
    [16]
    LUO L, ZHANG Q, KONG X, et al. Differential effects of bisphenol A toxicity on oyster (Crassostrea angulata) gonads as revealed by label-free quantitative proteomics[J]. Chemosphere, 2017, 176: 305-314. doi: 10.1016/j.chemosphere.2017.02.146
    [17]
    ZHANG L, SUN W, ZHANG Z, et al. Gender-specific metabolic responses in gonad of mussel Perna viridis to triazophos[J]. Mar Pollut Bull, 2017, 123(1/2): 39-46.
    [18]
    CORTES-DIAZ M J A, RODRIGUEZ-FLORES J, CASTANEDA-PENALVO G, et al. Sublethal effects induced by captopril on Cyprinus carpio as determined by oxidative stress biomarkers[J]. Sci Total Environ, 2017, 605-606: 811-823. doi: 10.1016/j.scitotenv.2017.06.208
    [19]
    ELLMAN G L, COURTNEY K D, ANDRES V, et al. A new and rapid colorimetric determination of acetylcholinesterase activity[J]. Biochem Pharmacol, 1961, 7(2): 88-95. doi: 10.1016/0006-2952(61)90145-9
    [20]
    MORON M S, DEPIERRE J W, MANNERVIK B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver[J]. Biochim Biophys Acta, 1979, 582(1): 67-78. doi: 10.1016/0304-4165(79)90289-7
    [21]
    任加云, 潘鲁青, 苗晶晶. 苯并(a)芘和苯并(k)荧蒽混合物对栉孔扇贝毒理学指标的影响[J]. 环境科学学报, 2006, 26(7): 1150-1156.
    [22]
    AEBI H. Catalase in vitro[J]. Meth Enzymol, 1984, 105: 121-126. doi: 10.1016/S0076-6879(84)05016-3
    [23]
    OHKAWA H, OHISHI N, YAGI K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction[J]. Anal Biochem, 1979, 95(2): 351-358. doi: 10.1016/0003-2697(79)90738-3
    [24]
    FULTON M H, KEY P B. Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects[J]. Environ Toxicol Chem, 2001, 20(1): 37-45. doi: 10.1002/etc.5620200104
    [25]
    DETRA R L, COLLINS W J. The relationship of parathion concentration, exposure time, cholinesterase inhibition and symptoms of toxicity in midge larvae (Chironomidae: Diptera)[J]. Environ Toxicol Chem, 1991, 10: 1089-1095. doi: 10.1002/etc.5620100814
    [26]
    BINELLI A, RICCIARDI F, RIVA C, et al. New evidences for old biomarkers: effects of several xenobiotics on EROD and AChE activities in zebra mussel (Dreissena polymorpha)[J]. Chemosphere, 2006, 62(4): 510-519. doi: 10.1016/j.chemosphere.2005.06.033
    [27]
    ALVES S, SEVERINO P, IBBOTSON D, et al. Effects of furadan in the brown mussel Perna perna and in the mangrove oyster Crassostrea rhizophorae[J]. Mar Environ Res, 2002, 54(3/4/5): 241-245. doi: 10.1016/S0141-1136(02)00138-1
    [28]
    LIONETTO M G, CARICATO R, GIORDANO M E, et al. Integrated use of biomarkers (acetylcholinesterase and antioxidant enzymes activities) in Mytillus galloprovincialis and Mullus barbatus in an Italian coastal marine area[J]. Mar Pollut Bull, 2003, 46(3): 324-330. doi: 10.1016/S0025-326X(02)00403-4
    [29]
    CHOI J Y, YU J, YANG D B, et al. Acetylthiocholine (ATC) cleaving cholinesterase (ChE) activity as a potential biomarker of pesticide exposure in the Manila clam, Ruditapes philippinarum, of Korea[J]. Mar Environ Res, 2011, 71(3): 162-168. doi: 10.1016/j.marenvres.2010.12.007
    [30]
    VALDEZ DOMINGOS F X, AZEVEDO M, SILVA M D, et al. Multibiomarker assessment of three Brazilian estuaries using oysters as bioindicators[J]. Environ Res, 2007, 105(3): 350-363. doi: 10.1016/j.envres.2007.06.003
    [31]
    DORAN W J, COPE W G, RADA R G, et al. Acetylcholinesterase inhibition in the threeridge mussel (Amblema plicata) by chlorpyrifos: implications for biomonitoring[J]. Ecotoxicol Environ Saf, 2001, 49(1): 91-98. doi: 10.1006/eesa.2000.2036
    [32]
    余向阳, 赵于丁, 王冬兰, 等. 毒死蜱和三唑磷对斑马鱼头部AChE活性影响及在鱼体内的富集[J]. 农业环境科学学报, 2008, 27(6): 2452-2455. doi: 10.3321/j.issn:1672-2043.2008.06.058
    [33]
    SINGH S, TIWARI R K, PANDEY R S. Acute toxicity evaluation of triazophos, deltamethrin and their combination on earthworm, Eudrilus eugeniae and its impact on AChE activity[J]. J Chem Ecol, 2019, 35(6): 563-575. doi: 10.1080/02757540.2019.1600679
    [34]
    刘海涛, 李兵, 王国宝, 等. 辛硫磷对家蚕雌雄个体的毒力比较[J]. 江苏农业科学, 2010, 6: 390-392. doi: 10.3969/j.issn.1002-1302.2010.02.160
    [35]
    刘海涛. 家蚕对有机磷农药抗性的性别差异研究[D]. 苏州: 苏州大学, 2010: 47-46.
    [36]
    郑波, 石年, 李龙, 等. 螨胺磷在不同性别大鼠中代谢差异初探[J]. 同济医科大学学报, 2000, 29(2): 127-129.
    [37]
    MAHARAJAN K, MUTHULAKSHMI S, NATARAJ B, et al. Toxicity assessment of pyriproxyfen in vertebrate model zebrafish embryos (Danio rerio): a multi biomarker study[J]. Aquat Toxicol, 2018, 196: 132-145. doi: 10.1016/j.aquatox.2018.01.010
    [38]
    ZHANG J F, WANG X R, GUO H Y, et al. Effects of water soluble fractions of diesel oil on the antioxidant defenses of the goldfish, Carassius auratus[J]. Ecotoxicol Environ Saf, 2004, 58(1): 110-116. doi: 10.1016/j.ecoenv.2003.08.025
    [39]
    钟碧瑾, 姚丹, 刘娟娟, 等. 两种有机磷农药对沼水蛙蝌蚪抗氧化系统及MDA浓度的影响[J]. 福建师范大学学报(自然科学版), 2009, 25(2): 91-96.
    [40]
    XIU M, PAN L, JIN Q. Toxic effects upon exposure to polycyclic aromatic hydrocarbon (chrysene) in scallop Chlamys farreri during the reproduction period[J]. Environ Toxicol Phar, 2016, 44: 75-83. doi: 10.1016/j.etap.2016.04.001
    [41]
    吉成龙. 典型溴系阻燃剂对紫贻贝毒理效应的组学研究[D]. 烟台: 中国科学院烟台海岸带研究所, 2014: 107-109.
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