Citation: | HUANG Guangzhong, HU Hui, XIAO Keyu, LUO Shimin, XIE Peirong, OUYANG Juying. Effects of plant extract from grape seed and sweet wormwood on intestinal digestive enzyme activities and blood biochemical parameters of eel (Monopterus albus)[J]. South China Fisheries Science, 2013, 9(2): 70-75. DOI: 10.3969/j.issn.2095-0780.2013.02.012 |
To explore the growth promotion mechanism of plant extract from grape (Vitis amurensis) seed and sweet wormwood on Monopterus albus, 2 400 healthy eel fingerlings were randomly divided into five groups.We fed control group with basal feed, and four experimental groups with diets containing respectively 10 g·kg-1, 20 g·kg-1, 30 g·kg-1, 40 g·kg-1 mixture, which were extracted from grape seed and from sweet wormwood(Artemisia annua) with a four-to-one proportion.We determined the digestive enzyme activities and blood biochemical parameters of five eel groups after feeding for 100 d in 21~28℃.Results show that basal diets with the mixture could improve digestive enzyme, blood glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT)′s activities, and total protein (TP)′s content; and could reduce blood glucose (GLU) and triglyceride (TG)′s contents of eel.Compared with the control group, the above indexes had significant difference (P < 0.05) when the dosage of the plant extract was 20 g·kg-1 or 30 g·kg-1, while the above indexes had no significant difference (P > 0.05) when it was 10 g·kg-1or 40 g·kg-1.There was no significant difference between 20 g·kg-1 and 30 g·kg-1 experimental groups.In conclusion, extract from grape seed and sweet wormwood with a four-to-one ratio, when mixed in diets with dosage 20~30 g·kg-1, could increase digestive enzyme activities, regulate blood biochemical parameters, enhance the digestion and metabolic activity of 3 main nutritive materials and improve the growth of fish.
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