Volume 11 Issue 3
Jun.  2015
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WANG Xiaomei, CHEN Chengxun, XING Kezhi, WANG Yan, DAI Wei, XUE Suqing. Analysis of daily weight gain and GH mRNA expression in juvenile Clarias gariepinu reared at different stocking densities[J]. South China Fisheries Science, 2015, 11(3): 35-40. DOI: 10.3969/j.issn.2095-0780.2015.03.006
Citation: WANG Xiaomei, CHEN Chengxun, XING Kezhi, WANG Yan, DAI Wei, XUE Suqing. Analysis of daily weight gain and GH mRNA expression in juvenile Clarias gariepinu reared at different stocking densities[J]. South China Fisheries Science, 2015, 11(3): 35-40. DOI: 10.3969/j.issn.2095-0780.2015.03.006
shu

Analysis of daily weight gain and GH mRNA expression in juvenile Clarias gariepinu reared at different stocking densities

  • Tianjin Key Lab. of Aqua-Ecology & Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

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  • Received Date: September 17, 2014
  • Revised Date: November 10, 2014
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  • Juvenile Clarias gariepinus were reared at densities of 35 kg · m-3, 65 kg · m-3, 95 kg · m-3 and 125 kg · m-3 for 60 d. On 30th and 60th day, we measured the daily weight gain (DWG) and assayed the mRNA level of GH in pituitary by semi-quantitative RT-PCR. During 0~30 d breeding, the DWGs showed no significant difference between 35 kg · m-3 and 65 kg m-3 treatments as well as between 95 kg · m-3 and 125 kg m-3 treatments, but the DWGs were significantly higher at 35 kg · m-3 and 65 kg · m-3 treatments than those at 95 kg · m-3 and 125 kg · m-3 treatments. While on 30th day, significant difference was not found in the GH mRNA level of pituitary between any two stocking densities. During 30~60 d breeding, the DWGs of 35 kg · m-3 and 65 kg · m-3 treatments were significantly higher than that of 125 kg · m-3 treatment, there was no significant difference in DWG between the other two treatments. On 60th day, the expressions of GH gene were significantly higher in 35 kg · m-3 and 65 kg · m-3 treatments than those in 95 kg · m-3 and 125 kg · m-3 treatments. The results indicate that there was no close correlation between DWG and mRNA level of GH in pituitary of juvenile C.gariepinus.

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  • [1]
    李文笙, 林浩然. 鱼类生长激素合成与分泌的内分泌调控网络: 垂体生长激素分泌细胞中的信号整合[J]. 中国科学, 2010, 40(2): 149-158. doi: 10.1360/zc2010-40-2-149
    [2]
    马细兰, 张勇, 黄卫人, 等. 尼罗罗非鱼生长激素及其受体的cDNA克隆与mRNA表达雌雄差异[J]. 动物学报, 2006, 52(5): 924-933. https://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_dwxb200605014
    [3]
    DENG S P, WU B, ZHU C H, et al. Molecular cloning and dimorphic expression of growth hormone (gh) in female and male spotted scat Scatophagus argus[J]. Fish Sci, 2014, 80(4): 715-723. doi: 10.1007/s12562-014-0763-5
    [4]
    LYNN S G, POWELL K A, WESTNEAT D F, et al. Seasonal and sex-specific mRNA levels of key endocrine genes in adult yellow perch (Perca flavescens) from Lake Erie[J]. Mar Biotechnol, 2009, 11(2): 210-222. doi: 10.1007/s10126-008-9136-3
    [5]
    温海深, 林浩然, 肖东. 野生鲇鱼生长激素分泌的季节变化及其神经内分泌调控[J]. 动物学报, 2002, 48(2): 213-220. https://www.doc88.com/p-0764364038983.html
    [6]
    FIGUEROA J, MARTÍN R S, FLORES C, et al. Seasonal modulation of growth hormone mRNA and protein levels in carp pituitary: evidence for two expressed genes[J]. J Comp Physiol B, 2005, 175(3): 185-192. doi: 10.1007/s00360-005-0474-4
    [7]
    王德寿, 林浩然, 张为民. 大鳍鳠脑垂体和血清生长激素水平的季节变化[J]. 水产学报, 1999, 23(1): 1-5. http://scxuebao.ijournals.cn/scxuebao/article/abstract/19990101
    [8]
    SHIN H S, LEE J, CHOI C Y. Effects of LED light spectra on the growth of the yellowtail clownfish Amphiprion clarkia[J]. Fish Sci, 2012, 78(3): 549-556. doi: 10.1007/s12562-012-0482-8
    [9]
    李云, 蔡生力. 半胱胺对斜带石斑鱼(Epinephelus coioides)生长激素及其受体mRNA水平的影响[J]. 海洋与湖沼, 2011, 42(2): 244-250. https://www.doc88.com/p-378487383742.html
    [10]
    马细兰, 张勇, 周立斌, 等. LHRH-RA对尼罗罗非鱼生长及生长轴相关基因表达的影响[J]. 水生生物学报, 2013, 37(1): 42-47. doi: 10.7541/2013.42
    [11]
    Van de NIEUWEGIESSEN P G, BOERLAGE A S, VERRETH J A J, et al. Assessing the effects of a chronic stressor, stocking density, on welfare indicators of juvenile African catfish, Clarias gariepinus Burchell[J]. Appl Anim Behav Sci, 2008, 115(3/4): 233-243. https://www.researchgate.net/publication/276973582_Assessing_the_effects_of_a_chronic_stressor_stocking_density_on_welfare_indicators_of_juvenile_African_catfish_Clarias_gariepinus_Burchell
    [12]
    Van de NIEUWEGIESSEN P G, OLWO J, KHONG S, et al. Effects of age and stocking density on the welfare of African catfish, Clarias gariepinus Burchell[J]. Aquaculture, 2009, 288(1/2): 69-75. https://research.wur.nl/en/publications/effects-of-age-and-stocking-density-on-the-welfare-of-african-cat
    [13]
    HOSFELD C D, HAMMER J, HANDELAND S O, et al. Effects of fish density on growth and smoltification in intensive production of Atlantic salmon (Salmo salar L. )[J]. Aquaculture, 2009, 294(3/4): 236-241. https://www.semanticscholar.org/paper/Effects-of-fish-density-on-growth-and-in-intensive-Hosfeld-Hammer/c6bf3bf7e16af4688990cc9a7b8250bf6eb9c26c
    [14]
    LUPATSCH I, SANTOS G A, SCHRAMA J W, et al. Effect of stocking density and feeding level on energy expenditure and stress responsiveness in European sea bass Dicentrarchus labrax[J]. Aquaculture, 2010, 298(3/4): 245-250. https://www.semanticscholar.org/paper/Effect-of-stocking-density-and-feeding-level-on-and-Lupatsch-Santos/8f1a5aaf83806f568a5a0974bdd5d4b3fe6178e2
    [15]
    SANTOS G A, SCHRAMA J W, MAMAUAG R E P, et al. Chronic stress impairs performance, energy metabolism and welfare indicators in European seabass (Dicentrarchus labrax): the combined effects of fish crowding and water quality deterioration[J]. Aquaculture, 2010, 299(1/2/3/4): 73-80. https://research.wur.nl/en/publications/chronic-stress-impairs-performance-energy-metabolism-and-welfare-
    [16]
    TOLUSSI C E, HILSDORF A W S, CANEPPELE D, et al. The effects of stocking density in physiological parameters and growth of the endangered teleost species piabanha, Brycon insignis (Steindachner, 1877)[J]. Aquaculture, 2010, 310(1/2): 221-228. https://www.mdpi.com/2076-3921/11/7/1215
    [17]
    DAI W, WANG X M, GUO Y J. Growth performance, hematological and biochemical responses of African catfish (Clarias gariepinus) reared at different stocking densities[J]. Afr J Agric Res, 2011, 28(6): 6177-6182. https://www.researchgate.net/publication/281008830_Growth_performance_hematological_and_biochemical_responses_of_African_catfish_Clarias_gariepinus_reared_at_different_stocking_densities
    [18]
    王晓梅, 戴伟, 郭永军, 等. 高密度养殖下革胡子鲶生长激素mRNA表达的半定量分析[J]. 华北农学报, 2011, 26(1): 94-98. https://www.doc88.com/p-2999598529779.html
    [19]
    FUKADA H, OZAKI Y, PIERCE A L, et al. Salmon growth hormone receptor: molecular cloning, ligand specificity, and response to fasting[J]. Gen Comp Endocrinol, 2004, 139(1): 61-71. doi: 10.1016/j.ygcen.2004.07.001
    [20]
    SAERA-VILA A, CALDUCH-GINER J A, PÉREZ-SÁNCHEZ J. Duplication of growth hormone receptor (GHR) in fish genome: gene organization and transcriptional regulation of GHR typeⅠand Ⅱ in gilthead sea bream (Sparus aurata)[J]. Gen Comp Endocrinol, 2005, 142(1/2): 193-203. https://pubmed.ncbi.nlm.nih.gov/15862563/
    [21]
    BRIAN C S, CHRISTOPHER A M, GEOFFREY C W, et al. Reduction in channel catfish hepatic growth hormone receptor expression in response to food deprivation and exogenous cortisol[J]. Domest Anim Endocrinol, 2006, 31(4): 340-356. doi: 10.1016/j.domaniend.2005.12.003
    [22]
    华益民, 林浩然. 营养状况对幼年鲤鱼肝脏IGF-ⅠmRNA表达的影响[J]. 动物学报, 2001, 47(1): 94-100. https://www.doc88.com/p-7899756958026.html
    [23]
    田娟, 涂玮, 曾令兵, 等. 饥饿和再投喂期间尼罗罗非鱼生长、血清生化指标和肝胰脏生长激素、类胰岛素生长因子-Ⅰ和胰岛素mRNA表达丰度的变化[J]. 水产学报, 2012, 36(6): 900-907. https://www.doc88.com/p-1817571150317.html
    [24]
    姜巨峰, 张殿昌, 邱丽华. 等. 用IGF-ⅠmRNA表达量评价鲮饲料配方效果的研究[J]. 南方水产, 2010, 6(2): 66-72. doi: 10.3969/j.issn.1673-2227.2010.02.011
    [25]
    强俊, 杨弘, 王辉, 等. 温度与饲料蛋白质水平对吉富品系尼罗罗非鱼(Oreochromis nilotica) 幼鱼生长和血清生长激素水平的影响[J]. 动物营养学报, 2012, 24(8): 1589-1601. doi: 10.3969/j.issn.1006-267x.2012.08.026
    [26]
    黄国强, 李洁, 唐夏, 等. 温度胁迫及恢复过程中褐牙鲆幼鱼GH、IGF-Ⅰ、RNA/DNA比值和糖原的变化[J]. 南方水产科学, 2012, 8(6): 16-21. doi: 10.3969/j.issn.2095-0780.2012.06.003
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