ZHANG Dianchang, HUANG Yanqin, SU Tianfeng, LI Jianzhu, ZHU Caiyan, JIANG Shigui. The effect of recombinant growth hormone(GH) on insulin-like growth factor-Ⅰ(IGF-Ⅰ) expression in mud carp, Cirrhinus molitorella[J]. South China Fisheries Science, 2008, 4(2): 50-55.
Citation: ZHANG Dianchang, HUANG Yanqin, SU Tianfeng, LI Jianzhu, ZHU Caiyan, JIANG Shigui. The effect of recombinant growth hormone(GH) on insulin-like growth factor-Ⅰ(IGF-Ⅰ) expression in mud carp, Cirrhinus molitorella[J]. South China Fisheries Science, 2008, 4(2): 50-55.
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The effect of recombinant growth hormone(GH) on insulin-like growth factor-Ⅰ(IGF-Ⅰ) expression in mud carp, Cirrhinus molitorella

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  • Expression patterns of IGF-ⅠmRNA in different tissues of mud carp （Cirrhinus molitorella）were studied using semi-quantitative RT-PCR method and the effects of recombinant mud carp growth hormone（rmcGH）on expression of IGF-Ⅰin mud carp were investigated. The IGF-ⅠmRNA was detected successfully in liver, kidney, brain, intestine, gill, spleen, gonad, and heart, and no IGF-ⅠmRNA was detected in skin and muscle. The IGF-Ⅰ mRNA detected was higher in liver than in other tissues. Mud carp were received either intra-peritoneal injections of rmcGH (120 gg-1 body weight) or vehicle, tissue samples and blood were collected 12 hour later. Total RNA was isolated and assayed for IGF-ⅠmRNA using semi-quantitative RT-PCR. Blood was extracted to determine the levels of IGF-Ⅰ in serum. IGF-Ⅰlevels increased from 145.5921.84 to 247.712.83 ngmL-1 (P=0.043) in the serum after rmcGH injection.
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