Volume 4 Issue 2
Apr.  2008
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ZHANG Dianchang, HUANG Yanqin, SU Tianfeng, LI Jianzhu, ZHU Caiyan, JIANG Shigui. The effect of recombinant growth hormone (GH)on insulin-like growth factor-Ⅰ (IGF-Ⅰ) expression in mud carp, Cirrhinus molitorella[J]. South China Fisheries Science, 2008, 4(2): 50-55.
Citation: ZHANG Dianchang, HUANG Yanqin, SU Tianfeng, LI Jianzhu, ZHU Caiyan, JIANG Shigui. The effect of recombinant growth hormone (GH)on insulin-like growth factor-Ⅰ (IGF-Ⅰ) expression in mud carp, Cirrhinus molitorella[J]. South China Fisheries Science, 2008, 4(2): 50-55.
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The effect of recombinant growth hormone (GH)on insulin-like growth factor-Ⅰ (IGF-Ⅰ) expression in mud carp, Cirrhinus molitorella

  • South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

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  • Received Date: November 06, 2007
  • Revised Date: December 28, 2007
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    • Abstract

  • Expression patterns of IGF-ⅠmRNA in different tissues of mud carp (Cirrhinus molitorella)were studied using semi-quantitative RT-PCR method and the effects of recombinant mud carp growth hormone(rmcGH)on expression of IGF-Ⅰin mud carp were investigated. The IGF-ⅠmRNA was detected successfully in liver, kidney, brain, intestine, gill, spleen, gonad, and heart, and no IGF-ⅠmRNA was detected in skin and muscle. The IGF-Ⅰ mRNA detected was higher in liver than in other tissues. Mud carp were received either intra-peritoneal injections of rmcGH (120 μg·g-1 body weight) or vehicle, tissue samples and blood were collected 12 hour later. Total RNA was isolated and assayed for IGF-ⅠmRNA using semi-quantitative RT-PCR. Blood was extracted to determine the levels of IGF-Ⅰ in serum. IGF-Ⅰlevels increased from 145.59±21.84 to 247.71±2.83 ng·mL-1 (P=0.043) in the serum after rmcGH injection.

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    • References (26)
  • [1]
    LI Wensheng, LIN Haoran, WONG A O L. Effects of gonadotropin-releasing hormone on growth hormone secretion and gene expression in common carp pituitary[J]. Comp Biochem Physiol: Part B, 2002, 132(2): 335-341. doi: 10.1016/S1096-4959(02)00039-8
    [2]
    LI Yinghua, BAI Junjie, JIAN Qing, et al. Expression of common carp growth hormone in the yeast Pichia pastoris and growth stimulation of juvenile tilapia (Oreochromis niloticus)[J]. Aquac, 2003, 216(13): 329-341. doi: 10.1016/S0044-8486(02)00406-4
    [3]
    江世贵, 张殿昌, 苏天凤, 等. 鲮生长激素cDNA的分子克隆和序列分析[J]. 中国水产科学, 2003, 10(2): 97-101. doi: 10.3321/j.issn:1005-8737.2003.02.003
    [4]
    KOJI Inoue, HOZI Iwatani, YOSHIO Takei. Growth hormone and insulin-like growth factorⅠof a Euryhaline fish Cottus kazika: cDNA cloning and expression after seawater acclimation[J]. Gen Comp Endocrinol, 2003, 131(1): 77-84. doi: 10.1016/S0016-6480(02)00650-0
    [5]
    BIGA P R, PETERSon B C, SCHELLING G T, et al. Bovine growth hormone treatment increased IGF-Ⅰin circulation and induced the production of a specific immune response in rainbow trout (Oncorhynchus mykiss)[J]. Aquac, 2005, 246(1/4): 437-445. doi: 10.1016/j.aquaculture.2005.01.019
    [6]
    张殿昌, 江世贵, 苏天凤, 等. 鲮胰岛素生长因子Ⅰ(IGF-Ⅰ)cDNA的分子克隆和序列分析[J]. 上海水产大学学报, 2002, 11(2): 97-101. doi: 10.3969/j.issn.1004-7271.2002.02.001
    [7]
    黄燕琴, 张殿昌, 苏天凤, 等. 重组鲮IGF-Ⅰ对鲮GH表达的影响[J]. 南方水产, 2006, 2(5): 19-24. doi: 10.3969/j.issn.2095-0780.2006.05.004
    [8]
    ZHANG Dianchang, HUANG Yanqin, SHAO Yanqing, et al. Molecular cloning, recombinant expression and growth-promoting effect of mud carp (Cirrhinus molitorella) insulin-like growth factor-Ⅰ[J]. Gen Comp Endocrinol, 2006, 148(2): 203-212. doi: 10.1016/j.ygcen.2006.03.014
    [9]
    SHINGO Kajimura, KATSUHISA Uchida, TAKASHI Yada, et al. Effects of insulin-like growth factors (IGF-Ⅰand-Ⅱ)on growth hormone and prolactin release and gene expression in euryhaline tilapia, Oreochromis mossambicus[J]. Gen Comp Endocrinol, 2002, 127(3): 223-231. doi: 10.1016/s0016-6480(02)00055-2
    [10]
    BIGA P R, GERALD T, SCHELLINGA R, et al. The effects of recombinant bovine somatotropin (rbST)on tissue IGF-Ⅰ, IGF-Ⅰreceptor, and GH mRNA levels in rainbow trout, Oncorhynchus mykiss[J]. Gen Comp Endocrinol, 2004, 135(3): 324-333. doi: 10.1016/j.ygcen.2003.10.014
    [11]
    VONG Q P, CHAN K M, CHENG C H K. Quantification of recombinant of mud carp IGF-Ⅰand IGF-ⅡmRNA by realtime PCR: differential regulation of expression by GH[J]. J Endocrinol, 2003, 17(8): 513-521. doi: 10.1677/joe.0.1780513
    [12]
    KELLEY K M, DESAI P, ROTH J T, et al. Evolution of endocrine growth regulation: the insulin like growth factors (IGFs), their regulatory binding proteins (IGFBPs), and IGF receptors in fishes and other ectothermic vertebrates[M]//FINGERMAN M, THOMPSON M F, NAGABHUSHANAM R. Recent advances in marine biotechnology. New Delhi: Oxford and IBH Publishing, 2000: 189-228. https://www.semanticscholar.org/paper/Evolution-of-endocrine-growth-regulation%3A-the-like-Kelley-Desai/66de760e9df4bc320b7df3b39e8bc4c24dc1dfed
    [13]
    MORIYAMA S, AYSon F G, KAWAUCHI H. Growth regulation by insulin-like growth factor-Ⅰin fish[J]. Biosci Biotechnol Biochem Rev, 2000, 64(11): 1 553-1 562. doi: 10.1271/bbb.64.1553
    [14]
    PEREZ-SANCHEZ J. The involvement of growth hormone in growth regulation, energy homeostasis and immune function in the gilthead sea bream (Sparus aurata): a short review[J] Fish Physiol Biochem, 2000, 22(10): 135-144. doi: 10.1023/A:1007816015345
    [15]
    THISSEN J P, UNDERWOOD L E, KETELSLEGERS J M. Regulation of insulin-like growth factor-Ⅰin starvation and injury[J]. Nutr Rev, 1999, 57(6): 167-176. doi: 10.1111/j.1753-4887.1999.tb06939.x
    [16]
    HASHIMOTO H, MIKAWA S, TAKAYAMA E, et al. Molecular cloning and growth hormone-regulated gene expression of carp insulin-like growth factor-Ⅰ[J]. Biochem Mol Biol Int, 1997, 41(5): 877-886. doi: 10.1080/15216549700201921
    [17]
    FUNKENSTEIN B, SILBERGELD A, CAVARI B, et al. Growth hormone increases plasma levels of insulin-like growth factor-Ⅰ(IGF-Ⅰ) in a teleost, the gilthead seabream (Sparus aurata)[J]. J Endocrinol, 1989, 120(7): 19-21. doi: 10.1677/joe.0.120r019
    [18]
    MORIYAMA S. Increased plasma insulin-like growth hormone factor-Ⅰ (IGF-Ⅰ) following oral and intraperitoneal administration of growth hormone to raimbow trout, Oncorhynchus mykiss[J]. Growth Regul, 1995, 53(3): 164-167. https://pubmed.ncbi.nlm.nih.gov/7580868/
    [19]
    CAO Q P. Nucleotide sequence and growth hormone regulated expression of salmon insulin-like growth factor Ⅰ mRNA[J]. Mol Endocrinol, 1989, 3(3): 2 006-2 010. doi: 10.1210/mend-3-12-2005
    [20]
    SCHMID A C, REINECKE M, KLOAS W. Primary cultured hepatocytes of the bony fish, Oreochromis mossambicus, the tilapia: a valid tool for physiological studieson IGF-Ⅰexpression in liver[J]. J Endocrinol, 2000, 166(2): 265-273. doi: 10.1677/joe.0.1660265
    [21]
    DUAN C, DUGUAY S J, PLISETSKAYA E M. Hormonal regulation of insulin-like growth factorⅠ(IGF-Ⅰ) mRNA expression in coho salmon[J]. Am Zool, 1992, 32: 13-20.
    [22]
    TSE M C L, VONG Q P, CHENG C H K, et al. PCR cloning and gene expression studies in common carp (Cyprinus carpio) insulin-like growthfactor-Ⅱ[J]. Biochimica Biophysical Acta, 2002, 1 575(1/3): 63-74. doi: 10.1016/s0167-4781(02)00244-0
    [23]
    PETEZ-SANCHEZ J, WEIL C, LE-BAI P Y. Effects of human insulin-like growth hormone factor-Ⅰon release of growth hormone by rainbow trout (Oncorhynchus mykiss) pituitary cells[J]. J Exp Zool, 1992, 262(3): 287-290. doi: 10.1002/jez.1402620308
    [24]
    LEEDOM T A, UCHIDA K, YADA T, et al. Recombinant bovine growth hormone treatment of tilapia: growth response, metabolic clearance, receptor binding and immunoglobulin production[J]. Aquac, 2002, 207(3/4): 359-380. doi: 10.1016/S0044-8486(01)00767-0
    [25]
    KAJIMURA S, UCHIDA K, YADA T, et al. Stimulation of insulin-like growth factor-Ⅰproduction by recombinant bovine growth hormone in Mozambique tilapia, Oreochromis mossambicus[J]. Fish Physiol Biochem, 2001, 25(3): 221-230. doi: 10.1023/A:1022268811599
    [26]
    SILVERSTEIN J T, WOLTERS W R, SHIMIZU M, et al. Bovine growth hormone treatment of channel catfish: strain and temperature effectson growth, plasma IGF-Ⅰlevels, feed intake and efficiency, and body composition[J]. Aquac, 2000, 190(1): 77-88. doi: 10.1016/S0044-8486(00)00387-2
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